Resumption of Meiotic Maturation of Oocytes, Pre- and Post-Implantational Embryonic Mortality under Conditions of Experimental Glomerulonephritis and Treatment of Silver Nanoparticles

Background: In recent years the use of silver nanoparticles (AgNP) has increased significantly being focused on assessing human health and environmental risks of nanotechnology.Methods: Research (two series) has been done on white laboratory mice. One dose (20 mg/kg) has been investigated. Frequency of treatment: one time per day for 10 times. Material for the study (ovaries, tubes and uterus) was taken under anesthetic anesthesia on the 10/11th, 21/22nd, 33/34th, 42/43rd days (after the last treatment). Oocyte meiotic maturation, pre- and post-implantation mortality of embryos under ten-time intravenous treatment of AgNP were investigation.Results: Under the ten-time treatment of AgNPs, the inhibition of reproductive function in female mice occurs: a decrease in the number and quality of ovarian oocytes; after male mice were planted, the females do not get pregnant to 21/22 day. The reproductive function in experimental animals is restored on the 40th day after the last treatment with AgNPs; there is no differences between the values of pre- and post-implantation mortality of embryos on the 33/34th day after male mice were planted; in one of the three experimental animals, 7 live pups were born on the 42/43rd day after the male was planted (in animals of the control group during this period: twice (6 ± 1 (n = 6) live pups).Conclusions: In mice females, under a ten-time treatment of AgNPs, the inhibition of reproductive function takes place; with the termination of the AgNPs treatment, the reproductive function is restored.

Download Full-text

Functional status of reproductive system under treatment of silver nanoparticles in female mice

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20171930 ◽

2017 ◽

Vol 6 (5) ◽

pp. 1713 ◽

Cited By ~ 1

Author(s):

Alina P. Lytvynenko ◽

Ludmila S. Rieznichenko ◽

Valentine A. Sribna ◽

Maria I. Stupchuk ◽

Nataliya G. Grushka ◽

...

Keyword(s):

Silver Nanoparticles ◽

Functional Status ◽

Reproductive System ◽

Contractile Activity ◽

Study Groups ◽

Environmental Risks ◽

Meiotic Maturation ◽

Follicular Cells ◽

Intravenous Treatment ◽

Post Implantation

Background: The use of silver nanoparticles (AgNP) has increased very significantly in recent years the work in science is currently on focused on assessing human health and environmental risks of nanotechnology. The aim of the present study was to estimate the functional status of the female reproductive system in mice under condition of the intravenous treatment of silver nanoparticles (AgNPs), namely to assess meiotic maturation of oocytes, viability of follicular cells surrounding the oocyte, spontaneous contractile activity of the myometrium and pre-and post-implantation mortality of embryos.Methods: Research (two series) has been done on white laboratory mice (8 weeks, 16-18 g). AgNPs are spherical nanoparticles of 30 nm (8 mg/ml for metal) diluted in water for injection. Method of treatment: intravenous. Two doses (2 mg/kg and 4 mg/kg) have been investigated. Frequency of treatment: one time per day with each dose of 1, 5 and 10 times (n=10 animals in each group). Material for the study (ovaries, uterus) were taken the day after the last AgNPs injection.Results: Ten-time AgNPs treatment (2 mg/kg and 4 mg/kg) results in inhibition of oocytes meiotic maturation in mice; a single- and five-time AgNPs treatment (2 mg/kg and 4 mg/kg) increases the number of apoptotic cells, while the ten-time AgNPs treatment results in an increase of the apoptotic and necrotic follicular cells surrounding oocytes; for the five-and ten-time AgNPs treatment (4 mg/kg) the index of contractility (IC) of the uterus increased; for the ten-time AgNPs treatment (2 mg/kg and 4 mg/kg) no differences in value of the embryonic mortality between control and study groups have been observed.Conclusions: This study suggests that the development of nanomaterials should be safer and non-toxic and the potential reprotoxicity of AgNPs should be investigated more carefully.

Download Full-text

Synthesis, performance and growth mechanism of silver nanoparticle coated SERS fiber probe

Matériaux & Techniques ◽

10.1051/mattech/2018061 ◽

2019 ◽

Vol 107 (3) ◽

pp. 305

Author(s):

Mengmei Geng ◽

Yuting Long ◽

Tongqing Liu ◽

Zijuan Du ◽

Hong Li ◽

...

Keyword(s):

Silver Nanoparticles ◽

Absorption Spectrum ◽

Reaction Time ◽

Growth Mechanism ◽

Fiber Optic ◽

Fiber Optic Probe ◽

Visible Absorption ◽

Fiber Probe ◽

Surface Enhanced Raman ◽

Optic Probe

Surface-enhanced Raman Scattering (SERS) fiber probe provides abundant interaction area between light and materials, permits detection within limited space and is especially useful for remote or in situ detection. A silver decorated SERS fiber optic probe was prepared by hydrothermal method. This method manages to accomplish the growth of silver nanoparticles and its adherence on fiber optic tip within one step, simplifying the synthetic procedure. The effects of reaction time on phase composition, surface plasmon resonance property and morphology were investigated by X-ray diffraction analysis (XRD), ultraviolet-visible absorption spectrum (UV-VIS absorption spectrum) and scanning electron microscope (SEM). The results showed that when reaction time is prolonged from 4–8 hours at 180 °C, crystals size and size distribution of silver nanoparticles increase. Furthermore, the morphology, crystal size and distribution density of silver nanoparticles evolve along with reaction time. A growth mechanism based on two factors, equilibrium between nucleation and growth, and the existence of PVP, is hypothesized. The SERS fiber probe can detect rhodamin 6G (R6G) at the concentration of 10−6 M. This SERS fiber probe exhibits promising potential in organic dye and pesticide residue detection.

Download Full-text

Assessment of the bactericidal effect of green synthesized silver nanoparticles against a panel of infectious microorganisms

Planta Medica ◽

10.1055/s-0036-1596752 ◽

2016 ◽

Vol 81 (S 01) ◽

pp. S1-S381

Author(s):

E Antunes ◽

M Mmola ◽

M Meyer ◽

DR Beukes

Keyword(s):

Silver Nanoparticles ◽

Bactericidal Effect ◽

Green Synthesized Silver Nanoparticles

Download Full-text

Regulation of spontaneous and induced resumption of meiosis in mouse oocytes by different intracellular pathways

Reproduction ◽

10.1530/reprod/120.2.377 ◽

2000 ◽

pp. 377-383 ◽

Cited By ~ 13

Author(s):

L Leonardsen ◽

A Wiersma ◽

M Baltsen ◽

AG Byskov ◽

CY Andersen

Keyword(s):

Signal Transduction ◽

Protein Kinase ◽

Protein Kinase A ◽

Mitogen Activated Protein Kinase ◽

Meiotic Maturation ◽

Mouse Oocytes ◽

Mitogen Activated Protein ◽

Dependent Signal ◽

Kinase Pathway ◽

Kinase A

The mitogen-activated protein kinase-dependent and the cAMP-protein kinase A-dependent signal transduction pathways were studied in cultured mouse oocytes during induced and spontaneous meiotic maturation. The role of the mitogen-activated protein kinase pathway was assessed using PD98059, which specifically inhibits mitogen-activated protein kinase 1 and 2 (that is, MEK1 and MEK2), which activates mitogen-activated protein kinase. The cAMP-dependent protein kinase was studied by treating oocytes with the protein kinase A inhibitor rp-cAMP. Inhibition of the mitogen-activated protein kinase pathway by PD98059 (25 micromol l(-1)) selectively inhibited the stimulatory effect on meiotic maturation by FSH and meiosis-activating sterol (that is, 4,4-dimethyl-5alpha-cholest-8,14, 24-triene-3beta-ol) in the presence of 4 mmol hypoxanthine l(-1), whereas spontaneous maturation in the absence of hypoxanthine was unaffected. This finding indicates that different signal transduction mechanisms are involved in induced and spontaneous maturation. The protein kinase A inhibitor rp-cAMP induced meiotic maturation in the presence of 4 mmol hypoxanthine l(-1), an effect that was additive to the maturation-promoting effect of FSH and meiosis-activating sterol, indicating that induced maturation also uses the cAMP-protein kinase A-dependent signal transduction pathway. In conclusion, induced and spontaneous maturation of mouse oocytes appear to use different signal transduction pathways.

Download Full-text