scholarly journals Chitosan from Lucilia cuprina (Diptera: Calliphoridae) enhances sensitization to insulin treatment in a diabetic burn mice of wound healing

2020 ◽  
Vol 1 (1) ◽  
pp. 1-15
Author(s):  
Lamia M. El-Samad ◽  
◽  
Azza A. Attia ◽  
Basant A. Bakr ◽  
◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Lucilia Cuprina ◽  
Diabetic Mice ◽  
Burn Wound ◽  
Time Intervals ◽  
Burn Wound Healing ◽  
High Degree

Chitosan is recognized as a multipurpose biomaterial because of its low allergenicity, non-toxicity, biodegradability and biocompatibility. The present study was designed to estimate the role of chitosan derived from Lucilia cuprina on burn healing in diabetic mice; using histopathological and microbiological studies at different time intervals. Chitosan was prepared from L. cuprina with high molecular weight (MW) and high degree of deacetylation (DD) to evaluate its burn wound healing potential; skin burn closure assessment, histological and microbiological studies in vivo in male diabetic mice. Chitosan topical treatment was superior in wound closure acceleration; mainly in insulin injected group at all the time intervals. Additionally, earlier epidermal remodelling with mature and intense collagen deposition was encountered in all chitosan treated animals as well as non-diabetic burned animals. There was a significant delay in hair growth and poor epidermal remodelling with impairment of wound closure in diabetic groups. Moreover, chitosan treated groups assert the chitosan antibacterial effects with protecting the burn against contamination that hinders healing especially in this diabetic condition. Further researches needed to interpret effects of possible synergistic combination therapy.

Development and characterization of silk films for burn wound healing

2020 ◽  
Vol 24 (2) ◽  
pp. 131-146
Author(s):  
Sukumar Nachiappan ◽  
Lami Amanuel ◽  
Tewodros Agazie ◽  
Seyoum Bihonegn
Keyword(s):  
Wound Healing ◽  
Research Work ◽  
Polymeric Materials ◽  
Study Data ◽  
Burn Wound ◽  
Content Type ◽  
Burn Wound Healing ◽  
Biomedical Device ◽  
Silk Films

Purpose Wound healing is a dynamic process that relies on coordinated signaling molecules to succeed. Silk has proven to be a promising biomaterial for the development of a novel product. The purpose of the study is development of silk films, augmented functionality can be provided to silk by means of loading honey and recombinant human epidermal growth factor (rhEGF). Design/methodology/approach In this research work, the authors set out to explore possibilities of silk-based biomedical device development with particular attention to different fabrication strategies that can be leveraged for this purpose. They have produced a novel silk-based drug delivery material, in the form of silk films. Scanning electronic microscope was used to observe the morphology and the highly specific surface area. The structure was studied by Fourier-transform infrared spectroscopy. This methodology is accomplished using in vivo study data using Wister albonia rats. Findings The developed films also provided a significant higher healing rate in vivo, with well-formed epidermis with faster granulation tissue formation when compared to the controls. Biodegradable polymeric materials based on blending aqueous dispersions of natural polymer sodium alginate, Chitosan and rhEGF complex, which allow controlled antiseptic release, are presented. Originality/value These results suggest that silk-based controlled release of Chitosan-rhEGF may serve as a new therapy to accelerate healing of burn wounds.

Role of Thymus Oil in Burn Wound Healing

2003 ◽  
Vol 24 (6) ◽  
pp. 395-399 ◽  
Author(s):  
Nurcan Dursun ◽  
Narin Liman ◽  
??rfan ??zyazgan ◽  
I??in G??ne?? ◽  
Recep Saraymen
Keyword(s):  
Wound Healing ◽  
Burn Wound ◽  
Burn Wound Healing

scholarly journals Injectable Nanocurcumin-Formulated Chitosan-g-Pluronic Hydrogel Exhibiting a Great Potential for Burn Treatment

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Le Hang Dang ◽  
Thi Hiep Nguyen ◽  
Ha Le Bao Tran ◽  
Vu Nguyen Doan ◽  
Ngoc Quyen Tran
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Healing Process ◽  
Nanocomposite Hydrogel ◽  
Burn Wound ◽  
Burn Treatment ◽  
Nanocomposite Hydrogels ◽  
Burn Wound Healing

Burn wound healing is a complex multifactorial process that relies on coordinated signaling molecules to succeed. Curcumin is believed to be a potent antioxidant and anti-inflammatory agent; therefore, it can prevent the prolonged presence of oxygen free radicals which is a significant factor causing inhabitation of optimum healing process. This study describes an extension of study about the biofunctional nanocomposite hydrogel platform that was prepared by using curcumin and an amphiphilic chitosan-g-pluronic copolymer specialized in burn wound healing application. This formular (nCur-CP, nanocomposite hydrogel) was a free-flowing sol at ambient temperature and instantly converted into a nonflowing gel at body temperature. In addition, the storage study determined the great stability level of nCur-CP in long time using UV-Vis and DLS. Morphology and distribution of nCur in its nanocomposite hydrogels were observed by SEM and TEM, respectively. In vitro studies suggested that nCur-CP exhibited well fibroblast proliferation and ability in antimicrobacteria. Furthermore, second- and third-degree burn wound models were employed to evaluate the in vivo wound healing activity of the nCur-CP. In the second-degree wound model, the nanocomposite hydrogel group showed a higher regenerated collagen density and thicker epidermis layer formation. In third degree, the nCur-CP group also exhibited enhancement of wound closure. Besides, in both models, the nanocomposite material-treated groups showed higher collagen content, better granulation, and higher wound maturity. Histopathologic examination also implied that the nanocomposite hydrogel based on nanocurcumin and chitosan could enhance burn wound repair. In conclusion, the biocompatible and injectable nanocomposite scaffold might have great potential to apply for wound healing.

scholarly journals Corrigendum to: The Role of Autologous PRP on Deep Partial-Thickness Burn Wound Healing in Bama Pigs

2021 ◽  
Author(s):  
Yanwei Sun ◽  
Yongqian Cao ◽  
Ran Zhao ◽  
Famei Xu ◽  
Dan Wu ◽  
...  
Keyword(s):  
Wound Healing ◽  
Burn Wound ◽  
Burn Wound Healing ◽  
Partial Thickness ◽  
Partial Thickness Burn

scholarly journals 3D bioprinting of integral ADSCs-NO hydrogel scaffolds to promote severe burn wound healing

2021 ◽  
Vol 8 (3) ◽  
Author(s):  
Yu Wu ◽  
Tangzhao Liang ◽  
Ying Hu ◽  
Shihai Jiang ◽  
Yuansen Luo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Umbilical Vein ◽  
3D Bioprinting ◽  
Severe Burn ◽  
Burn Wound ◽  
Hydrogel Scaffold ◽  
Hydrogel Scaffolds ◽  
Burn Wound Healing ◽  
Severe Burns

Abstract Severe burns are challenging to heal and result in significant death throughout the world. Adipose-derived mesenchymal stem cells (ADSCs) have emerged as a promising treatment for full-thickness burn healing but are impeded by their low viability and efficiency after grafting in vivo. Nitric oxide (NO) is beneficial in promoting stem cell bioactivity, but whether it can function effectively in vivo is still largely unknown. In this study, we bioprinted an efficient biological scaffold loaded with ADSCs and NO (3D-ADSCs/NO) to evaluate its biological efficacy in promoting severe burn wound healing. The integral 3D-ADSCs/NO hydrogel scaffolds were constructed via 3D bioprinting. Our results shown that 3D-ADSCs/NO can enhance the migration and angiogenesis of Human Umbilical Vein Endothelial Cells (HUVECs). Burn wound healing experiments in mice revealed that 3D-ADSCs/NO accelerated the wound healing by promoting faster epithelialization and collagen deposition. Notably, immunohistochemistry of CD31 suggested an increase in neovascularization, supported by the upregulation of vascular endothelial growth factor (VEGF) mRNA in ADSCs in the 3D biosystem. These findings indicated that 3D-ADSC/NO hydrogel scaffold can promote severe burn wound healing through increased neovascularization via the VEGF signalling pathway. This scaffold may be considered a promising strategy for healing severe burns.

scholarly journals Role of caveolin-1 in epidermal stem cells during burn wound healing in rats

2019 ◽  
Vol 445 (2) ◽  
pp. 271-279 ◽  
Author(s):  
Ronghua Yang ◽  
Jingru Wang ◽  
Ziheng Zhou ◽  
Shaohai Qi ◽  
Shubin Ruan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Wound Healing ◽  
Epidermal Stem Cells ◽  
Burn Wound ◽  
Caveolin 1 ◽  
Burn Wound Healing

Fabrication, Physical Characterizations, and In Vitro, In Vivo Evaluation of Ginger Extract-Loaded Gelatin/Poly(Vinyl Alcohol) Hydrogel Films Against Burn Wound Healing in Animal Model

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
Barkat Ali Khan ◽  
Shahid Ullah ◽  
Muhammad Khalid Khan ◽  
Bushra Uzair ◽  
Farid Menaa ◽  
...  
Keyword(s):  
Wound Healing ◽  
Animal Model ◽  
Poly Vinyl Alcohol ◽  
Burn Wound ◽  
Ginger Extract ◽  
In Vivo Evaluation ◽  
Burn Wound Healing ◽  
Hydrogel Films

scholarly journals The plasminogen receptor, Plg-RKT, plays a role in inflammation and fibrinolysis during cutaneous wound healing in mice

2020 ◽  
Vol 11 (12) ◽  
Author(s):  
Lina Ny ◽  
Robert J. Parmer ◽  
Yue Shen ◽  
Sandra Holmberg ◽  
Nagyung Baik ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Closure ◽  
Cytokine Expression ◽  
Epidermal Differentiation ◽  
Epidermal Thickness ◽  
Burn Wound ◽  
Burn Wounds ◽  
Burn Wound Healing ◽  
Key Steps ◽  
Wound Tissue

AbstractWound healing is a complex physiologic process that proceeds in overlapping, sequential steps. Plasminogen promotes fibrinolysis and potentiates the inflammatory response during wound healing. We have tested the hypothesis that the novel plasminogen receptor, Plg-RKT, regulates key steps in wound healing. Standardized burn wounds were induced in mice and time dependence of wound closure was quantified. Healing in Plg-RKT−/− mice was significantly delayed during the proliferation phase. Expression of inflammatory cytokines was dysregulated in Plg-RKT−/− wound tissue. Consistent with dysregulated cytokine expression, a significant delay in wound healing during the proliferation phase was observed in mice in which Plg-RKT was specifically deleted in myeloid cells. Following wound closure, the epidermal thickness was less in Plg-RKT−/− wound tissue. Paradoxically, deletion of Plg-RKT, specifically in keratinocytes, significantly accelerated the rate of healing during the proliferation phase. Mechanistically, only two genes were upregulated in Plg-RKT−/− compared with Plg-RKT+/+ wound tissue, filaggrin, and caspase 14. Both filaggrin and caspase 14 promote epidermal differentiation and decrease proliferation, consistent with more rapid wound closure and decreased epidermal thickness during the remodeling phase. Fibrin clearance was significantly impaired in Plg-RKT−/− wound tissue. Genetic reduction of fibrinogen levels to 50% completely abrogated the effect of Plg-RKT deletion on the healing of burn wounds. Remarkably, the effects of Plg-RKT deletion on cytokine expression were modulated by reducing fibrinogen levels. In summary, Plg-RKT is a new regulator participating in different phases of cutaneous burn wound healing, which coordinately plays a role in the interrelated responses of inflammation, keratinocyte migration, and fibrinolysis.

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