scholarly journals Injectable Nanocurcumin-Formulated Chitosan-g-Pluronic Hydrogel Exhibiting a Great Potential for Burn Treatment

2018 ◽  
Vol 2018 ◽  
pp. 1-14 ◽  
Author(s):  
Le Hang Dang ◽  
Thi Hiep Nguyen ◽  
Ha Le Bao Tran ◽  
Vu Nguyen Doan ◽  
Ngoc Quyen Tran
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Healing Process ◽  
Nanocomposite Hydrogel ◽  
Burn Wound ◽  
Burn Treatment ◽  
Nanocomposite Hydrogels ◽  
Burn Wound Healing

Burn wound healing is a complex multifactorial process that relies on coordinated signaling molecules to succeed. Curcumin is believed to be a potent antioxidant and anti-inflammatory agent; therefore, it can prevent the prolonged presence of oxygen free radicals which is a significant factor causing inhabitation of optimum healing process. This study describes an extension of study about the biofunctional nanocomposite hydrogel platform that was prepared by using curcumin and an amphiphilic chitosan-g-pluronic copolymer specialized in burn wound healing application. This formular (nCur-CP, nanocomposite hydrogel) was a free-flowing sol at ambient temperature and instantly converted into a nonflowing gel at body temperature. In addition, the storage study determined the great stability level of nCur-CP in long time using UV-Vis and DLS. Morphology and distribution of nCur in its nanocomposite hydrogels were observed by SEM and TEM, respectively. In vitro studies suggested that nCur-CP exhibited well fibroblast proliferation and ability in antimicrobacteria. Furthermore, second- and third-degree burn wound models were employed to evaluate the in vivo wound healing activity of the nCur-CP. In the second-degree wound model, the nanocomposite hydrogel group showed a higher regenerated collagen density and thicker epidermis layer formation. In third degree, the nCur-CP group also exhibited enhancement of wound closure. Besides, in both models, the nanocomposite material-treated groups showed higher collagen content, better granulation, and higher wound maturity. Histopathologic examination also implied that the nanocomposite hydrogel based on nanocurcumin and chitosan could enhance burn wound repair. In conclusion, the biocompatible and injectable nanocomposite scaffold might have great potential to apply for wound healing.

scholarly journals RELEVANCE AND PERSPECTIVES OF EXPERIMENTAL WOUND MODELS IN WOUND HEALING RESEARCH

2017 ◽  
Vol 10 (7) ◽  
pp. 57 ◽  
Author(s):  
Santram Lodhi ◽  
Gautam P Vadnere
Keyword(s):  
Wound Healing ◽  
Search Engines ◽  
Wound Repair ◽  
Healing Process ◽  
Chorioallantoic Membrane ◽  
Tube Formation ◽  
Wound Healing Process ◽  
Burn Wound

The wound healing process consists of four highly integrated and overlapping phases: Hemostasis, inflammation, proliferation, and tissue remodeling. These phases and their biophysiological functions must occur in the proper sequence, at a specific time and continue for a specific duration at an optimal intensity. There are many factors that can affect wound healing which interferes with one or more phases in this process, thus causing improper or impaired tissue repair. This review was aimed to collect data and made a critical analysis. This will provide concise information regarding different models and parameters used for wound healing study. The data related to different wound models are collected using popular search engines as well as relevant science search engines and database including Google Scholar, Science Direct, and PubMed. A new drug substance can be evaluated for wound healing activity using different in vitro models such as cell culture, chick chorioallantoic membrane model, tube formation on metrigel and capillary growth model. The in vivo wound models such as incision, excision, dead space, burn wound, ischemic wound, and diabetic wound models are frequently used. Each model has specific importance. The limitations and advantages of each are described in this review. Although animal wound repair is an imperfect reflection of human wound healing and its clinical challenges, these models can be fundamental tools for the development of new approaches to rational wound therapy. 

scholarly journals Systemic and topical administration of spermidine accelerates skin wound healing

2020 ◽  
Author(s):  
Daisuke Ito ◽  
Hiroyasu Ito ◽  
Takayasu Ideta ◽  
Ayumu Kanbe ◽  
Soranobu Ninomiya ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Wound Repair ◽  
Healing Process ◽  
Topical Administration ◽  
Skin Wound ◽  
Wound Healing Process ◽  
Skin Wound Healing

Abstract Background The skin wound healing process is regulated by various cytokines, chemokines, and growth factors. Recent reports have demonstrated that spermine/spermidine (SPD) promote wound healing through urokinase-type plasminogen activator (uPA)/uPA receptor (uPAR) signaling in vitro. Here, we investigated whether the systemic and topical administration of SPD would accelerate the skin wound-repair process in vivo.Methods A skin wound repair model was established using C57BL/6 J mice. SPD was mixed with white petrolatum for topical administration. For systemic administration, SPD mixed with drinking water was orally administered. Changes in wound size over time were calculated using digital photography.Results Systemic and topical SPD treatment significantly accelerated skin wound healing. The administration of SPD promoted the uPA/uPAR pathway in wound sites. Moreover, topical treatment with SPD enhanced the expression of IL-6 and TNF-α in wound sites. Scratch and cell proliferation assays revealed that SPD administration accelerated scratch wound closure and cell proliferation in vitro.Conclusion These results indicate that treatment with SPD promotes skin wound healing through activation of the uPA/uPAR pathway and induction of the inflammatory response in wound sites. The administration of SPD might contribute to new effective treatments to accelerate skin wound healing.

scholarly journals A 3D In Vitro Model for Burn Wounds: Monitoring of Regeneration on the Epidermal Level

Biomedicines ◽  
2021 ◽  
Vol 9 (9) ◽  
pp. 1153
Author(s):  
Verena Schneider ◽  
Daniel Kruse ◽  
Ives Bernardelli de Mattos ◽  
Saskia Zöphel ◽  
Kendra-Kathrin Tiltmann ◽  
...  
Keyword(s):  
Wound Healing ◽  
Inflammatory Response ◽  
Healing Process ◽  
Three Dimensional ◽  
Source Model ◽  
Burn Wound ◽  
Thermal Burn ◽  
Burn Wounds

Burns affect millions every year and a model to mimic the pathophysiology of such injuries in detail is required to better understand regeneration. The current gold standard for studying burn wounds are animal models, which are under criticism due to ethical considerations and a limited predictiveness. Here, we present a three-dimensional burn model, based on an open-source model, to monitor wound healing on the epidermal level. Skin equivalents were burned, using a preheated metal cylinder. The healing process was monitored regarding histomorphology, metabolic changes, inflammatory response and reepithelialization for 14 days. During this time, the wound size decreased from 25% to 5% of the model area and the inflammatory response (IL-1β, IL-6 and IL-8) showed a comparable course to wounding and healing in vivo. Additionally, the topical application of 5% dexpanthenol enhanced tissue morphology and the number of proliferative keratinocytes in the newly formed epidermis, but did not influence the overall reepithelialization rate. In summary, the model showed a comparable healing process to in vivo, and thus, offers the opportunity to better understand the physiology of thermal burn wound healing on the keratinocyte level.

scholarly journals Platelet-Rich Plasma-Derived Exosomal USP15 Promotes Cutaneous Wound Healing via Deubiquitinating EIF4A1

2021 ◽  
Vol 2021 ◽  
pp. 1-14
Author(s):  
Yan Xu ◽  
Ze Lin ◽  
Lei He ◽  
Yanzhen Qu ◽  
Liu Ouyang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Platelet Rich Plasma ◽  
Healing Process ◽  
Cell Counting ◽  
Wound Healing Process ◽  
Hacat Cells ◽  
Epithelial Regeneration

Epithelial regeneration is an essential wound healing process, and recent work suggests that different types of exosomes (Exos) can improve wound repair outcomes by promoting such epithelial regeneration. Platelet-rich plasma (PRP) is known to facilitate enhanced wound healing, yet the mechanisms underlying its activity are poorly understood. To explore these mechanisms, we first isolated PRP-derived Exos (PRP-Exos). Using immortalized keratinocytes (HaCaT cells) treated with PBS, PRP, or PRP-Exos, we conducted a series of in vitro Cell Counting Kit-8 (CCK-8), EdU, scratch wound, and transwell assays. We then established a wound defect model in vivo in mice and assessed differences in the mRNA expression within these wounds to better understand the basis for PRP-mediated wound healing. The functions of PRP-Exos and USP15 in the context of wound healing were then confirmed through additional in vitro and in vivo experiments. We found that PRP-Exos effectively promoted the in vitro proliferation, migration, and wound healing activity of HaCaT cells. USP15 was further identified as a key mediator through which these PRP-Exos were able to promote tissue repair both in vitro and in vivo. At a mechanistic level, USP15 enhanced the functional properties of HaCaT cells by promoting EIF4A1 deubiquitination. Thus, PRP-Exos and USP15 represent promising tools that can promote wound healing via enhancing epithelial regeneration.

Peptide amphiphilic (PA) gels enhance burn wound healing in vitro and in vivo

2015 ◽  
Vol 221 (4) ◽  
pp. e121 ◽  
Author(s):  
Kameron Rezzadeh ◽  
Situo Zhou ◽  
Akishige Hokugo ◽  
Zheyu Zhang ◽  
Luis A. Segovia ◽  
...  
Keyword(s):  
Wound Healing ◽  
Burn Wound ◽  
Burn Wound Healing

scholarly journals Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Pawel Olczyk ◽  
Katarzyna Komosinska-Vassev ◽  
Grzegorz Wisowski ◽  
Lukasz Mencner ◽  
Jerzy Stojko ◽  
...  
Keyword(s):  
Wound Healing ◽  
Healing Process ◽  
Silver Sulfadiazine ◽  
Wound Healing Process ◽  
Burn Wound ◽  
Burn Treatment ◽  
Burn Wound Healing ◽  
Fibronectin Expression ◽  
The Matrix ◽  
Healing Wounds

The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronectin components was also assessed by an electrophoresis in polyacrylamide gel, with a subsequent immunodetection by Western Blotting. Propolis burn treatment decreased the release of fibronectin components from healing wounds in relation to damages treated with silver sulfadiazine. The main reason of decreased extraction of fibronectin components from wounds treated with propolis was a substantial decrease of degradation product release of the mentioned glycoprotein, which was observed particularly from the 3rd to 5th day of the repair. Wounds treatment with propolis demonstrated, especially in relation to damages treated with silver sulfadiazine, the decreased release of synthesized fibronectin molecules. The obtained results suggest that propolis modifies fibronectin metabolism in the course of wound healing process. The influence of propolis is reflected in prevention of fibronectin biosynthesis as well as its degradation in the wound area. The above-mentioned metabolic changes may decrease the risk of complications in the repair wounds process.

scholarly journals INJECTABLE NANOCURCUMIN–FORMULATED CHITOSAN-G-PLURONIC HYDROGEL EXHIBITING A GREAT POTENTIAL FOR BURN TREATMENT

2018 ◽  
Vol 56 (5) ◽  
Author(s):  
Dinh Van Tuan
Keyword(s):  
Wound Healing ◽  
Chronic Wounds ◽  
Aqueous Dispersion ◽  
Pluronic F127 ◽  
Nanocomposite Hydrogel ◽  
Burn Wound ◽  
Burn Treatment ◽  
Burn Wound Healing ◽  
Degree Burn ◽  
Long Time

Burns is one of the popular accidents today and usually leaves serious physical and mental damage. For long time represent acute wounds, burns are evolution into chronic wounds, if inadequately managed. Up to now, there have been abundancy of natural and synthetic products for burn healing. In the study, we fabricated a thermosensitive nanocomposite hydrogel in which incorporated dual active curcumin and chitosan. Beside of a well-known characteristic of chitosan for wound healing, curcumin has been a lot of interest in burn wound healing application due to ability in depleting the action of oxidative radicals and stimulation of fibroblast cells. In order to enhance the therapeutic efficacy of curcumin, we introduced the new method to synthesize nanocurcumin in the a thermosensitive chitosan-g-Pluronic F127 copolymer solution under ultrasonication. The rheology of aqueous solutions of this material is studied as a function of temperature. The solutions of this material undergo a transition to a gel at higher temperature, above which a complex rheological behavior is observed. In addition, a minimum inhibitory concentration of this material was determined for a variety of bacterial and was compared to that of curcumin. It was found that the aqueous dispersion of this material was much more effective than curcumin against both positive and negative gram bacterial. In the third degree burn models, the nCur-CP hydrogel performed a higher burn healing rate as compared to Silvirin-treated burn. These data suggest that the nanocomposite hydrogel may be great potential for burn treatment.

scholarly journals Omega-7 oil increases telomerase activity and accelerates healing of grafted burn and donor site wounds

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yosuke Niimi ◽  
Dannelys Pérez-Bello ◽  
Koji Ihara ◽  
Satoshi Fukuda ◽  
Sam Jacob ◽  
...  
Keyword(s):  
Wound Healing ◽  
Blood Flow ◽  
Keratinocyte Growth Factor ◽  
Donor Site ◽  
Telomerase Activity ◽  
Burn Wound ◽  
In Vitro Proliferation ◽  
Burn Wound Healing

AbstractThis study investigated the efficacy of Omega-7 isolated from the sea buckthorn oil (Polyvit Co., Ltd, Gangar Holding, Ulaanbaatar, Mongolia) in ovine burn wound healing models. In vitro, proliferation (colony-forming rate) and migration (scratch) assays using cultured primary ovine keratinocytes were performed with or without 0.025% and 0.08% Omega-7, respectively. The colony-forming rate of keratinocytes in the Omega-7 group at 72 and 96 h were significantly higher than in the control (P < 0.05). The percentage of closure in scratch assay in the Omega-7 group was significantly higher than in the control at 17 h (P < 0.05). In vivo, efficacy of 4% Omega-7 isolated from buckthorn oil was assessed at 7 and 14 days in grafted ovine burn and donor site wounds. Telomerase activity, keratinocyte growth factor, and wound nitrotyrosine levels were measured at day 14. Grafted sites: Un-epithelialized raw surface area was significantly lower and blood flow was significantly higher in the Omega-7-treated sites than in control sites at 7 and 14 days (P < 0.05). Telomerase activity and levels of keratinocyte growth factors were significantly higher in the Omega-7-treated sites after 14 days compared to those of control (P < 0.05). The wound 3-nitrotyrosine levels were significantly reduced by Omega-7. Donor sites: the complete epithelialization time was significantly shorter and blood flow at day 7 was significantly higher in the Omega-7-treated sites compared to control sites (P < 0.05). In summary, topical application of Omega-7 accelerates healing of both grafted burn and donor site wounds. Omega-7 should be considered as a cost-efficient and effective supplement therapy for burn wound healing.

scholarly journals Mechanism of Wound-Healing Activity ofHippophae rhamnoidesL. Leaf Extract in Experimental Burns

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Nitin K. Upadhyay ◽  
Ratan Kumar ◽  
M. S. Siddiqui ◽  
Asheesh Gupta
Keyword(s):  
Wound Healing ◽  
Wound Repair ◽  
Leaf Extract ◽  
Lipid Peroxide ◽  
Enzymatic Antioxidants ◽  
Burn Wound ◽  
Burn Wounds ◽  
Aqueous Leaf Extract

The present investigation was undertaken to evaluate the healing efficacy of lyophilized aqueous leaf extract of Sea buckthorn (Hippophae rhamnoidesL., family Elaeagnaceae) (SBT) and to explore its possible mechanism of action on experimental burn wounds in rats. The SBT extract, at various concentrations, was applied topically, twice daily for 7 days. Treatment with silver sulfadiazine (SSD) ointment was used as reference control. The most effective concentration of the extract was found to be 5.0% (w/w) for burn wound healing and this was further used for detailed study. The SBT-treated group showed faster reduction in wound area in comparison with control and SSD-treated groups. The topical application of SBT increased collagen synthesis and stabilization at the wound site, as evidenced by increase in hydroxyproline, hexosamine levels and up-regulated expression of collagen type-III. The histological examinations and matrix metalloproteinases (MMP-2 and -9) expression also confirmed the healing efficacy of SBT leaf extract. Furthermore, there was significant increase in levels of endogenous enzymatic and non-enzymatic antioxidants and decrease in lipid peroxide levels in SBT-treated burn wound granulation tissue. The SBT also promoted angiogenesis as evidenced by anin vitrochick chorioallantoic membrane model andin vivoup-regulated vascular endothelial growth factor (VEGF) expression. The SBT leaf extract had no cytotoxic effect on BHK-21 cell line. In conclusion, SBT aqueous leaf extract possesses significant healing potential in burn wounds and has a positive influence on the different phases of wound repair.

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