scholarly journals USO DE 5-FLUOROURACIL IMPACTA A PROPORÇÃO DE FIBRAS COLÁGENAS NA REGIÃO DA CABEÇA DO EPIDÍDIMO DE RATOS ADULTOS

2021 ◽  
Author(s):  
Letícia Ganem Rillo Paz Barateiro ◽  
Pedro Luiz Zonta De Freitas ◽  
Lilian Catarim Fabiano ◽  
Nilza Cristina Buttow ◽  
Jaqueline De Carvalho Rinaldi
Keyword(s):  
Picrosirius Red

Introdução: O uso do quimioterápico 5-fluorouracil (5-FU) pode causar toxicidade e danos colaterais a muitos órgãos. Todavia, há poucos estudos que investigam esses danos nos órgãos do sistema reprodutor masculino, por exemplo o epidídimo, o qual se destaca por armazenar e maturar os espermatozoides. Sabendo que a manutenção da histoarquitetura epididimária é importante para garantir seu desempenho funcional, faz-se relevante avaliar o colágeno presente na matriz extracelular devido ao seu papel estrutural. Objetivo: Avaliar o impacto do 5-FU nas fibras colágenas tipos I e III do epidídimo de ratos adultos. Material e métodos: Este trabalho recebeu aprovação pelo Comitê de Ética no Uso de Animais da Universidade Estadual de Maringá (protocolo: 4422140918). Quatorze ratos Wistar machos foram divididos em dois grupos (n=7): grupo controle (GC) e grupo quimioterapia (G5-FU). Os animais do G5-FU receberam, via intraperitoneal, o 5-FU por quatro dias consecutivos na dose de 15 mg/kg, com redução para 6 mg/kg por mais quatro dias alternados e dose final de 15 mg/kg. Já os animais do GC foram administrados com soro fisiológico pela mesma via e período referidos. No 15° dia, realizou-se a eutanásia dos ratos de ambos os grupos. O epidídimo foi dissecado, fixado em Methacarn e incluído em blocos de parafina para posterior realização de cortes semi-seriados de 5 μm. Estes foram corados com Picrosírius Red para realização da densitometria do colágeno na região da cabeça do epidídimo. Por fim, a análise estatística foi executada pelo teste Mann Whitney e os resultados expressos em porcentagem. Resultados: Houve diferença significativa (p<0,05) entre os grupos em relação à deposição das fibras colágenos tipo I, mas não às do tipo III. O colágeno tipo I ocupou aproximadamente 4,43% da área total no GC e 3,46% no G5-FU. Esses dados indicam que a quimioterapia impactou a organização da matriz extracelular, modificando a histoarquitetura epididimária. Mais estudos são necessários para compreender os mecanismos envolvidos bem como o reflexo disto na função do órgão. Conclusão: O 5-FU alterou a densitometria do colágeno na região da cabeça do epidídimo de ratos Wistar adultos.

The polarization colors of picrosirius red-stained collagen in systemic sclerosis.

1991 ◽  
Vol 53 (1) ◽  
pp. 82-85
Author(s):  
Nobuhiro YAMAMOTO ◽  
Mamoru KASADA ◽  
Yoichiro SASAI
Keyword(s):  
Systemic Sclerosis ◽  
Picrosirius Red

scholarly journals Combination of histochemical analyses and micro-MRI reveals regional changes of the murine cervix in preparation for labor

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Antara Chatterjee ◽  
Rojan Saghian ◽  
Anna Dorogin ◽  
Lindsay S. Cahill ◽  
John G. Sled ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Diffusion Tensor ◽  
Ex Vivo ◽  
Muscle Cells ◽  
Pregnant Mouse ◽  
Collagen Fibers ◽  
Muscular Layer ◽  
Tissue Organization ◽  
Picrosirius Red

AbstractThe cervix is responsible for maintaining pregnancy, and its timely remodeling is essential for the proper delivery of a baby. Cervical insufficiency, or “weakness”, may lead to preterm birth, which causes infant morbidities and mortalities worldwide. We used a mouse model of pregnancy and term labor, to examine the cervical structure by histology (Masson Trichome and Picrosirius Red staining), immunohistochemistry (Hyaluronic Acid Binding Protein/HABP), and ex-vivo MRI (T2-weighted and diffusion tensor imaging), focusing on two regions of the cervix (i.e., endocervix and ectocervix). Our results show that mouse endocervix has a higher proportion of smooth muscle cells and collagen fibers per area, with more compact tissue structure, than the ectocervix. With advanced gestation, endocervical changes, indicative of impending delivery, are manifested in fewer smooth muscle cells, expansion of the extracellular space, and lower presence of collagen fibers. MRI detected three distinctive zones in pregnant mouse endocervix: (1) inner collagenous layer, (2) middle circular muscular layer, and (3) outer longitudinal muscular layer. Diffusion MRI images detected changes in tissue organization as gestation progressed suggesting the potential application of this technique to non-invasively monitor cervical changes that precede the onset of labor in women at risk for preterm delivery.

scholarly journals Evaluation of Fibrotic Changes in OSMF:A Retrospective Study Using Special Stains and Polarizing Microscopy

2017 ◽  
Vol 6 (2) ◽  
pp. 1518
Author(s):  
Parvez Ikram ◽  
Nadeem Jeddy
Keyword(s):  
Connective Tissue ◽  
Large Scale ◽  
Oral Submucous Fibrosis ◽  
Muscle Layer ◽  
Inclusion Criteria ◽  
Polarizing Microscopy ◽  
Potentially Malignant ◽  
Tissue Changes ◽  
Submucous Fibrosis ◽  
Picrosirius Red

<p><strong>Background:</strong> Oral submucous fibrosis (OSMF), a potentially malignant oral disorder has the highest rate of malignant transformation of about 7-13%. The connective tissue changes that occur in this disease are characteristic and are stained with special stains.</p><p><strong>Objective:</strong> The study was done to compare common and special stains under light microscopy and polarizing microscopy to evaluate the levels of fibrosis in oral submucous fibrosis and assess the type of collagen present in the stromal area.</p><p><strong>Materials and Methods:</strong> Fifty tissue blocks were selected from the archives and were prepared and stained with H&amp;E, Masson's trichrome, Van Gieson and Picrosirius red and studied under light microscope and polarizing microscope respectively.</p><p><strong>Results:</strong> H and E stained slides were useful in diagnosing the lesion but was not able to highlight the level of fibrosis. Masson's trichrome and Van Gieson stained slides showed the depth of the lesion which extended even to the deeper muscle layer. The type of collagen present was definitively seen by the birefringence in polarizing microscopic study. Interobserver variation was less and all the values regarding the effectiveness of the special stains in detecting the level of fibrosis were statistically significant.</p><p><strong>Conclusion:</strong> Special stains can be used routinely in laboratories to demonstrate connective tissue lesions especially in cases of OSMF. Depth of the lesion and the area of involvement help in treatment planning to be delivered. Large scale studies with more categories and inclusion criteria are required along with the special stains to assess the other alterations in OSMF.</p>

scholarly journals Influência do óleo de cártamo (Carthamus tinctorius L.) na resistência insulina e hipertrofia ventricular de camundongos dislipidêmicos

2020 ◽  
Vol 9 (9) ◽  
pp. e243997141
Author(s):  
Karine de Paula Caproni ◽  
Ângela Maria Martins ◽  
Brigida Monteiro Villas Boas ◽  
Danielle Aparecida Quintino Silva Sarto ◽  
Aline Manke Nachtigall ◽  
...  
Keyword(s):  
Carthamus Tinctorius ◽  
Picrosirius Red

A regulação do metabolismo lipídico através do consumo de fitoterápicos é uma estratégia para reduzir o risco de doenças cardiovasculares. Diante disso, o objetivo do estudo foi avaliar o efeito do óleo de cártamo sobre a resistência à insulina e hipertrofia ventricular esquerda (HVE) em camundongos com ausência de receptor para LDL (LDLr-/-) com diferentes dietas. Os Camundongos LDLr-/- foram divididos em quatro grupos: C: dieta padrão; CCA: dieta padrão e 2mg/kg/dia de óleo de cártamo; HL: dieta hiperlipídica; HLCA: dieta hiperlipídica e 2mg/kg/dia de óleo de cártamo. Após 15 dias de experimento foi quantificado as concentrações séricas de colesterol total e suas frações, triglicerídeos, proteína C reativa, insulina, glicose e calculado o índice de Homa. Lâminas histológicas com tecido cardíaco foram preparadas e coradas com hematoxilina e eosina, para avaliação histológica geral; e coradas com picrosírius red para avaliar da área de depósito de colágeno. Os resultados obtidos demonstraram que o óleo de cártamo preveniu a redução dos níveis séricos do HDL e do aumento dos níveis da PCR, preveniu parcialmente a resistência insulínica e a hiperinsulinemia nos camundongos do grupo HLCA. O uso de óleo de cártamo equilibrou os fatores anti-hipertróficos em relação aos fatores hipertróficos (hiperinsulinemia e processo inflamatório), prevenindo a HVE nos camundongos do grupo HLCA. Em conclusão o óleo de cártamo preveniu o desenvolvimento da HVE e parcialmente a resistência à insulina.

scholarly journals Study of polarization colors in the connective tissue wall of odontogenic cysts using picrosirius red stain

2015 ◽  
Vol 7 (2) ◽  
pp. 119 ◽  
Author(s):  
Sandhya Tamgadge ◽  
Anusha Shetty ◽  
Avinash Tamgadge ◽  
Sudhir Bhalerao ◽  
Treville Periera ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Odontogenic Cysts ◽  
Picrosirius Red

Abstract 301: Nebivolol Attenuates Prooxidant and Profibrotic Mechanisms and Decreases Vascular Remodeling in Renovascular Hypertension

Hypertension ◽  
2013 ◽  
Vol 62 (suppl_1) ◽  
Author(s):  
Carla S Ceron ◽  
Elen Rizzi ◽  
Danielle A Guimaraes ◽  
Alisson Martins-Oliveira ◽  
Raquel F Gerlach ◽  
...  
Keyword(s):  
Vascular Remodeling ◽  
Adrenergic Receptor ◽  
Wistar Rats ◽  
Aortic Wall ◽  
Left Renal Artery ◽  
Male Wistar Rats ◽  
Receptor Blockers ◽  
First Time ◽  
Picrosirius Red ◽  
Metalloproteinase 9

Nebivolol and metoprolol are β1-adrenergic receptor blockers with different properties. We hypothesized that nebivolol, but not metoprolol, could attenuate prooxidant and profibrotic mechanisms of hypertension vascular remodeling. Hypertension was induced in male Wistar rats by clipping the left renal artery. Six weeks after surgery, hypertensive and sham rats were treated with nebivolol (Nebi 10 mg/kg/day), metoprolol (Meto 20 mg/kg/day) or vehicle for four weeks. Systolic blood pressure was monitored weekly. Morphologic changes in the aortic wall were studied in picrosirius red sections. Aortic NAD(P)H activity was evaluated by luminescence. Nitrotyrosine staining was evaluated to assess peroxynitrite formation by immunohistochemistry. TGF-β and matrix metalloproteinase-9 (MMP-9) levels were determined by immunofluorescence, and p-ERK 1/2 expression by western blotting. Both β1-receptor antagonists exerted very similar antihypertensive effects (156 ± 8 mmHg and 151 ± 9 mmHg, respectively, versus 206 ± 7 mmHg in hypertensive controls; both P<0.05). However, while metoprolol had no significant effects, nebivolol significantly (all P<0.05) attenuated vascular collagen surface (237944 ± 59567, 69784 ± 17686, 183215 ± 30338 μm2, respectively, in the 2K1C, 2K1C+Nebi, and 2K1C+Meto groups), attenuated hypertension-induced increases in aortic NAD(P)H oxidase activity (253887 ± 13712, 143765 ± 15642, and 232465 ± 14352 AU, respectively in the 2K1C, 2K1C+Nebi, and 2K1C+Meto groups), in nitrotyrosine levels (166.3 ± 2.9, 145.3 ± 1.5, 172.1 ± 7.3 AU, respectively, in the 2K1C, 2K1C+Nebi, and 2K1C+Meto groups), in TGF-β upregulation (7.2 ± 0.12, 6.5 ± 0.03, 7.0 ± 0.3 AU, respectively, in the 2K1C, 2K1C+Nebi, and 2K1C+Meto groups) and in MMP-9 levels (18.27 ± 0.8, 12.73 ± 0.4, 15.76 ± 1.4 AU, respectively in the 2K1C, 2K1C+Nebi, and 2K1C+Meto groups). No effects on p-ERK 1/2 expression were found with both drugs (P>0.05) (1.0 ± 0.16, 0.92 ± 0.15, 0.87 ± 0.37 AU, respectively, in the 2K1C, 2K1C+Nebi, and 2K1C+Meto groups). These results show for the first time that nebivolol, but not metoprolol, attenuates prooxidant and profibrotic mechanisms involving TGF-β and MMP-9, which promote vascular remodeling in hypertension.

scholarly journals Understanding the Lingual Frenulum: Histological Structure, Tissue Composition, and Implications for Tongue Tie Surgery

2020 ◽  
Vol 2020 ◽  
pp. 1-12
Author(s):  
Nikki Mills ◽  
Donna T. Geddes ◽  
Satya Amirapu ◽  
S. Ali Mirjalili
Keyword(s):  
Individual Variability ◽  
Histological Structure ◽  
Tissue Composition ◽  
En Bloc ◽  
Structural Explanation ◽  
Fresh Tissue ◽  
Floor Of Mouth ◽  
Variable Extent ◽  
The Individual ◽  
Picrosirius Red

Lingual frenotomy has become an increasingly common surgical procedure, performed for a broad range of indications from birth through adulthood. This study utilizes histology to define the structure and tissue composition of the lingual frenulum and floor of mouth (FOM) fascia. En bloc specimens of anterior tongue, lingual frenulum, and FOM tissues were harvested from ten embalmed adult cadavers. An additional three fresh tissue cadaveric specimens were frozen with the tongue supported in an elevated position, to enable harvesting and paraffin embedding of the elevated lingual frenulum as a discrete specimen. All 13 specimens were prepared as ten-micron coronal sections using stains to determine the general morphology of the lingual frenulum, its relationship to neighbouring structures (Mason’s Trichrome), presence of elastin fibers (Verhoeff-van Gieson), and collagen typing (Picrosirius Red). Our results have shown a submucosal layer of fascia spanning horizontally across the FOM was present in all specimens, with variability in fascial thickness and histologic composition. This FOM fascia suspends the sublingual glands, vessels, and genioglossus from its deep surface. The elevated lingual frenulum is formed by a central fold of this FOM fascia together with the overlying oral mucosa with variability in fascial thickness and composition. With tongue elevation, the fascia mobilizes to a variable extent into the fold forming the frenulum, providing a structural explanation for the individual variability in lingual frenulum morphology seen in clinical practice.

scholarly journals Digital Image Analysis of Picrosirius Red Staining: A Robust Method for Multi-Organ Fibrosis Quantification and Characterization

Biomolecules ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1585
Author(s):  
Guillaume E. Courtoy ◽  
Isabelle Leclercq ◽  
Antoine Froidure ◽  
Guglielmo Schiano ◽  
Johann Morelle ◽  
...  
Keyword(s):  
Digital Method ◽  
Computer Assisted ◽  
Kidney Fibrosis ◽  
Collagen Accumulation ◽  
Organ Specific ◽  
Picrosirius Red ◽  
Lung And Kidney ◽  
Organ Fibrosis ◽  
Accurate Quantification

Current understanding of fibrosis remains incomplete despite the increasing burden of related diseases. Preclinical models are used to dissect the pathogenesis and dynamics of fibrosis, and to evaluate anti-fibrotic therapies. These studies require objective and accurate measurements of fibrosis. Existing histological quantification methods are operator-dependent, organ-specific, and/or need advanced equipment. Therefore, we developed a robust, minimally operator-dependent, and tissue-transposable digital method for fibrosis quantification. The proposed method involves a novel algorithm for more specific and more sensitive detection of collagen fibers stained by picrosirius red (PSR), a computer-assisted segmentation of histological structures, and a new automated morphological classification of fibers according to their compactness. The new algorithm proved more accurate than classical filtering using principal color component (red-green-blue; RGB) for PSR detection. We applied this new method on established mouse models of liver, lung, and kidney fibrosis and demonstrated its validity by evidencing topological collagen accumulation in relevant histological compartments. Our data also showed an overall accumulation of compact fibers concomitant with worsening fibrosis and evidenced topological changes in fiber compactness proper to each model. In conclusion, we describe here a robust digital method for fibrosis analysis allowing accurate quantification, pattern recognition, and multi-organ comparisons useful to understand fibrosis dynamics.

Picrosirius red staining revisited

2011 ◽  
Vol 113 (3) ◽  
pp. 231-233 ◽  
Author(s):  
Raymond Coleman
Keyword(s):  
Picrosirius Red
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