Estabelecimento e Desenvolvimento Inicial In Vitro de Baruzeiro (Dipteryx Alata Vogel)

2021 ◽  
Author(s):  
MUZA DO CARMO VIEIRA ◽  
KERLY CRISTINA PEREIRA; GILSON DOURADO DA SILVA ◽  
LíDIA MARIA MORAIS MONTEIRO LAMIM; THIMóTEO PAES BARBOSA ◽  
MARIANA SILVA PEREIRA DE PAULA ◽  
ANDERSON DIAS VAZ DE SOUZA ◽  
...  
Keyword(s):  
Dipteryx Alata

Objetivou-se com esse trabalho estabelecer e desenvolver plântulas de Baruzeiro in vitro. As sementes de Baru foram coletadas em árvores adultas no município de Pires do Rio - GO, desinfestadas em água e detergente, álcool 70% e hipoclorito de sódio 2,5% e lavadas em água destilada autoclavada. Estas foram inoculadas em frascos de vidro contendo meio de cultura MS (Murashige e Skoog, 1962) 0,0 e 50% da concentração dos sais. Experimento 1, foi utilizado diferentes princípios ativos (hipoclorito de sódio e ácido acetilsalicílico - AAS) e diferentes concentrações (Hipoclorito de sódio (0; 0,25; 0,50; 0,75; e 1,00%) e AAS. E o Experimento 2 testando diferentes concentrações do meio MS (MS 0 e MS 50%). Avaliou-se o índice de oxidação; contaminação; à porcentagem de sobrevivência, o comprimento da raiz principal (cm); o comprimento da parte aérea (cm); o número de folhas e o diâmetro do caule (cm). Os experimentos foram instalados em delineamento inteiramente casualizado (DIC). Nas condições em que se realizou este estudo conclui-se que: o estabelecimento in vitro levando em consideração o índice de germinação e sobrevivência para os meios com AAS e NaClO foram de 80,0% e 64% (AAS) e 90,0% (NaClO). Quanto ao uso de MS 0% e MS 50% com carvão ativado, houve percentual médio de 71% de germinação. Para o meio MS 50% e carvão ativado houve percentual médio de 91% de germinação com o T2 com 100% de germinação. ,

scholarly journals Callus Induction in Baru (Dipteryx alata Vog.) Explants

2018 ◽  
Vol 47 (2) ◽  
pp. 538-543
Author(s):  
Rodrigo Kelson S. REZENDE ◽  
Ana Maria N. SCOTON ◽  
Maílson V. JESUS ◽  
Zeva V. PEREIRA ◽  
Fernanda PINTO
Keyword(s):  
Ascorbic Acid ◽  
Callus Induction ◽  
Callus Formation ◽  
Leaf Explants ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Alternative Technique ◽  
Propagation Methods ◽  
Dipteryx Alata

Baru (Dipteryx alata Vog.) is a species with great economic and environmental potential; it has popular acceptance, besides being a very productive species. Alternative propagation methods are important for species maintenance and exploration. Thus, micropropagation emerged as an alternative technique, providing genetic stability and the production of a large number of seedlings. The aim of the present investigation was to develop a callus induction protocol for in vitro baru explants. The tested explants were nodal, internodal and foliar segments. The explants were disinfected for 30 seconds in 70% alcohol (v/v) and 2 minutes in sodium hypochlorite (1.25% active chlorine). This was followed by triple washing. The inoculation was carried out in test tubes containing 15 mL MS medium (30 g L-1 sucrose, 6 g L-1 agar and 100 mg L-1 ascorbic acid) supplemented with 2.0 mg L-1 naphthalene acetic acid (NAA). The solution also contained 0.0, 2.5 or 5.0 mg L-1 of 6-benzylaminopurine (BAP) with the pH adjusted to 5.8. In the incubation phase, the explants were cultured for seven days in the dark and then subjected to a photoperiod of 16 hours (43 µmol m-2 s-1) at 25 ± 2 °C. The treatments were studied with 2.5, 5.0, 7.5 or 10.0 mg L-1 BAP additions to the MS. Callus formation, contamination and oxidation evaluations were undertaken. The results obtained when using 2.0 mg L-1 NAA concluded that such a treatment should be used to induce callogenesis from nodal explants, while for the tested baru leaf explants, the best results for callus formation were given by the combination of 2.0 mg L-1 NAA with 2.5 mg L-1 of BAP to.

scholarly journals In vitro establishment and early development of barueiro (Dipteryx alata Vogel)

2016 ◽  
Vol 37 (4) ◽  
pp. 1779 ◽  
Author(s):  
Herick Fernando de Jesus Silva ◽  
Simone Abreu Asmar ◽  
Rayssa Camargo de Oliveira ◽  
Berildo De Melo ◽  
José Magno Queiroz Luz ◽  
...  
Keyword(s):  
Activated Charcoal ◽  
Large Scale ◽  
Culture Media ◽  
Germination Rate ◽  
Woody Species ◽  
Chlorophyll Contents ◽  
Fruit Species ◽  
In Vitro Establishment ◽  
Dipteryx Alata

The barueiro (Dipteryx alata Vog.) is a native fruit species of the Cerrado ecoregion that has multiple uses. It is a wild species, and its cultivation is difficult. Furthermore, it is threatened with extinction. Plant tissue culture is a major tool for the conservation of germplasm, as well as a means of propagating high-quality seedlings on a large scale. However, this technique has not been used with barueiro, although it might provide valuable contributions to the process of barueiro domestication. The most popular method of cultivation is the use of the Murashige and Skoog medium (MS), which is considered one of the most nutritionally complete media. Woody plant medium (WPM) is indicated for the propagation of woody species, but there are no reports of its use for barueiro cultivation. Woody plants tend to have problems with rust in vitro during the establishment phase. Activated charcoal acts as an adjuvant for the adsorption of phenolic compounds, mitigating its effects in the medium. Thus, the objective of this study was to test four activated charcoal doses (0, 2, 4 and 6 g L-1) and three culture media: MS, WPM, and AA (over water agar) in the in vitro establishment of barueiro. The experimental design was a completely randomised (DIC), 4 × 3 factorial design with three replications. At 60 days after inoculation, the explants were evaluated for dry matter, fresh weight, stem diameter, shoot length, number of leaves, longest root length, germination rate, and chlorophyll contents. The MS medium supplemented with 3,0 g L-1 activated charcoal appeared to be the best for in vitro establishment of barueiro.

The Effect of Lupane Triterpenoids (Dipteryx alata Vogel) in the in vitro Neuromuscular Blockade and Myotoxicity of two Snake Venoms

2012 ◽  
Vol 16 (22) ◽  
pp. 2717-2723 ◽  
Author(s):  
Miriele Cristina Ferraz ◽  
Luciane Aparecida Celestino Parrilha ◽  
Maria Silvia Duarte Moraes ◽  
Jorge Amaral Filho ◽  
Jose Carlos Cogo ◽  
...  
Keyword(s):  
Neuromuscular Blockade ◽  
Snake Venoms ◽  
Lupane Triterpenoids ◽  
Dipteryx Alata

scholarly journals Baru Pulp (Dipteryx alata Vogel): Fruit from the Brazilian Savanna Protects against Oxidative Stress and Increases the Life Expectancy of Caenorhabditis elegans via SOD-3 and DAF-16

Biomolecules ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 1106
Author(s):  
Natasha Rios Leite ◽  
Laura Costa Alves de Araújo ◽  
Paola dos Santos da Rocha ◽  
Danielle Araujo Agarrayua ◽  
Daiana Silva Ávila ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Caenorhabditis Elegans ◽  
Life Expectancy ◽  
Biological Activities ◽  
Radical Scavenging ◽  
Brazilian Savanna ◽  
Beneficial Effects ◽  
Dipteryx Alata

Fruits are sources of bioactive compounds that are responsible for several biological activities. Therefore, this study aimed to identify the chemical composition of the pulp of the Brazilian Savanna fruit Dipteryx alata; evaluate its toxic effects, influence on the life expectancy of the nematode Caenorhabditis elegans, and its antioxidant activities in vitro and in vivo; and describe the mechanisms involved. The chemical compounds identified include phenols, terpenes, fatty acid derivatives, vitamins, and a carboxylic acid. The in vitro antioxidant activity was demonstrated by radical scavenging methods. in vivo, the D. alata fruit pulp was not toxic and promoted resistance to oxidative stress in nematodes exposed to a chemical oxidizing agent. Furthermore, it promoted an increased life expectancy in wild-type nematodes and increased the expression of superoxide dismutase and the nuclear translocation of DAF-16. These results suggest that the beneficial effects identified are related to these two genes, which are involved in the regulation of metabolic activities, the control of oxidative stress, and the lifespan of C. elegans. These beneficial effects, which may be related to its chemical constituents, demonstrate its potential use as a functional and/or nutraceutical food.

scholarly journals Study of the proteins in the defatted flour and protein concentrate of baru nuts (Dipteryx alata Vog)

2012 ◽  
Vol 32 (3) ◽  
pp. 464-470 ◽  
Author(s):  
Rita de Cássia Avellaneda Guimarães ◽  
Simone Palma Favaro ◽  
Antonio Camilo Arguelho Viana ◽  
José Antônio Braga Neto ◽  
Valdir Augusto Neves ◽  
...  
Keyword(s):  
Functional Properties ◽  
Protein Solubility ◽  
Absorption Capacity ◽  
In Vitro Digestibility ◽  
Protein Concentrate ◽  
Emulsifying Properties ◽  
Lipid Fractions ◽  
Sds Page ◽  
Dipteryx Alata

Baru (Dipteryx alata Vog.) is an abundant legume in the Brazilian Savanna. Its nuts can be exploited sustainably using its protein and lipid fractions. This study aimed to analyze the proteins of the nuts present in the defatted flour and protein concentrate in terms of their functional properties, the profile of their fractions, and the in vitro digestibility. The flour was defatted with hexane and extracted at the pH of higher protein solubility to obtain the protein concentrate. The electrophoretic profile of the protein fractions was evaluated in SDS-PAGE gel. The functional properties of the proteins indicate the possibility of their use in various foods, like soybeans providing water absorption capacity, oil absorption capacity, emulsifying properties, and foamability. Globulins, followed by the albumins, are the major fractions of the flour and protein concentrate, respectively. Digestibility was greater for the concentrate than for the defatted flour.

scholarly journals Concentration of ms medium and cutting of seeds on in vitro establishment of baruzeiro (Dipteryx alata Vog.)

2017 ◽  
pp. 306-313
Author(s):  
Hernane Fernandes Pinhal ◽  
Elequisandra da Costa Araruna ◽  
Pedro Augusto Porto Carneiro ◽  
Simone Abreu Asmar ◽  
Berildo de Melo ◽  
...  
Keyword(s):  
Ms Medium ◽  
In Vitro Establishment ◽  
Dipteryx Alata
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