scholarly journals ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF NIEBUHRIA APETALA (ROTH) DUNN – IN VITRO MODELS

2019 ◽  
pp. 278-281
Author(s):  
RAJESH A ◽  
DOSS A ◽  
TRESINA PS ◽  
MOHAN VR
Keyword(s):  
Methanol Extract ◽  
Protein Denaturation ◽  
In Vitro Models ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Inflammatory Agent ◽  
Potential Source ◽  
Anti Inflammatory

Objective: The objective of this study was to determine the anti-inflammatory activity of methanol extract of Niebuhria apetala and its possible mechanism of action. Methods: Methanol extract of Niebuhria apetala leaf (NAL) was assessed for its anti-inflammatory activity by in vitro methods. Using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, and antilipoxygenase activity at different concentrations, in vitro anti-inflammatory activity was estimated. The standard drug used for this purpose was aspirin. Results: Methanol extract NAL at a concentration range of 100–500 μg/ml significant (p<0.01) protects the heat-induced protein denaturation. At the concentration of 500 mg/ml, NAL showed significant (p<0.01) inhibition of protease inhibitory action. Heat-induced hemolysis of erythrocyte, hypotonicity-induced hemolysis, and lipooxygenase activity were significant (p<0.01) inhibited at the concentration of 500 μg/ml. Conclusion: Finally, the present study indicates that methanol extract of Niebuhria apetala can be a potential source of anti-inflammatory agent.

scholarly journals IN VITRO ANTI-INFLAMMATORY ACTIVITY OF 4-BENZYLPIPERIDINE

2016 ◽  
pp. 108 ◽  
Author(s):  
Jayashree V ◽  
Bagyalakshmi S ◽  
Manjula Devi K ◽  
Richard Daniel D
Keyword(s):  
Inhibitory Activity ◽  
Protein Denaturation ◽  
In Vitro Models ◽  
Inflammatory Activity ◽  
Significant Activity ◽  
Standard Drug ◽  
Study Results ◽  
Anti Inflammatory ◽  
Dose Dependent

<p>ABSTRACT<br />Objective: To study the in vitro anti-inflammatory activity of 4-benzylpiperidine.<br />Methods: This study was conducted to evaluate the in vitro anti-inflammatory activity of 4-benzylpiperidine using in vitro models such as inhibition<br />of albumin denaturation and proteinase inhibitory activity.<br />Results: This study revealed the dose-dependent inhibition of protein denaturation and proteinase inhibitory activity by 4-benzylpiperidine.<br />Conclusion: In the present study, results indicate that the 4-benzylpiperidine possess anti-inflammatory properties. The drug inhibited the heat<br />induced albumin denaturation and proteinase inhibitory activity. It shows dose-dependent significant activity when compared with a standard drug.<br />Hence, this study gives an idea that the 4-benzylpiperidine can be used as a lead compound for designing a potent anti-inflammatory drug which can<br />be used to cure inflammation.<br />Keywords: Anti-inflammatory activity, 4-Benzylpiperidine, Protein denaturation, Proteinase inhibitory activity.</p>

scholarly journals A scientific validation of In vitro anti-inflammatory activity of Punica granatum L. by human red blood cell membrane stabilization

2018 ◽  
Vol 6 (7) ◽  
pp. 2430
Author(s):  
Karunakar Kota ◽  
Sandhya Sharma ◽  
Jameela Tahashildar
Keyword(s):  
Methanolic Extract ◽  
Punica Granatum ◽  
Inflammatory Activity ◽  
Membrane Stabilization ◽  
Fruit Peel ◽  
Standard Drug ◽  
Membrane Lysis ◽  
Anti Inflammatory ◽  
Punica Granatum L

Background: In recent years there has been growing interest in therapeutic use of natural products, especially those derived from plants. P. granatum is very common dietary ingredient in many parts of India and has remarkable biological and medicinal properties.Methods: In the present study, the methanolic extract of fruit peels of Punica granatum Linn. (MEPG) were investigated for anti-inflammatory activity by simple, reliable, less toxic and less time consuming HRBC membrane stabilization method. The presentation of hypo tonicity induced HRBC membrane lysis was taken a measure of anti-inflammatory activity. Their activities were compared with standard drug diclofenac.Results: The results of the study demonstrated that P. granatum contains active constituents, which possess anti-inflammatory activity which is probably related to the inhibition of prostaglandin synthesis.Conclusions: It is concluded that methanolic extract of P. granatum fruit peel possesses significant anti-inflammatory activity and this is a possible rationale for its folkloric use as an anti-inflammatory agent.

scholarly journals Anti-inflammatory and Anti-arthritic Potentials of Maesa Montana Roots

2021 ◽  
Vol 18 (16) ◽  
Author(s):  
Sanjida SHARMIN ◽  
Rabeya Gazi JHUMA ◽  
Sanjida ISLAM ◽  
Riniara KHATUN
Keyword(s):  
Bovine Serum ◽  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Flowering Plant ◽  
Activity Measurement ◽  
Root Extract ◽  
Standard Drug ◽  
Activity Test ◽  
Anti Inflammatory

Maesa Montana is a flowering plant of the Myrsinaceae family, which is locally known as ramjani. The current study aimed to evaluate the in-vitro anti-arthritic and anti-inflammatory activities of the root extract of this plant. The anti-inflammatory performances were measured by the hindrance of egg white denaturation, and anti-arthritic activity was investigated by Bovine serum protein. In the anti-inflammatory activity test, the methanolic root extract of this plant showed 69.29 ± 1.19 % of inhibition at a concentration of 1000 µg/mL and standard drug exhibited 90.11 ± 1.45 % of inhibition at the same concentration. Furthermore, in the anti-arthritic activity test, the extract demonstrated 68.18 ± 1.34 % of inhibition at a concentration of 1000 µg/mL whereas the standard diclofenac drug showed 90.65 ± 1.19 % of inhibition at the same concentration. These results revealed that the root extract of this plant possesses significant anti-arthritic and anti-inflammatory activities based on the inhibition of BSA and protein denaturation. HIGHLIGHTS Methanol soluble compounds extraction from the roots of Maesa Montana Anti-inflammatory activity determination using egg albumin Anti-arthritic activity measurement using bovine serum albumin GRAPHICAL ABSTRACT

scholarly journals POTENTIAL ANTIOXIDANT, ANTI-INFLAMMATORY AND ANTIBACTERIAL EVALUATION OF EXTRACTS OF LEUCAS ASPERA USING IN VITRO MODELS

2016 ◽  
Vol 8 (12) ◽  
pp. 292 ◽  
Author(s):  
Tahareen S. ◽  
Shwetha R. ◽  
Myrene R. D.
Keyword(s):  
Inflammatory Activity ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Leucas Aspera ◽  
Ic50 Value ◽  
Anti Inflammatory ◽  
Stabilization Assay

<p><strong>Objective: </strong>To evaluate the potential antioxidant, anti-inflammatory and antibacterial activities of aqueous and methanolic extracts of leaves of <em>Leucas aspera</em> (Thumbae).</p><p><strong>Methods: </strong>Phytochemical screening of the leaves of <em>L. aspera</em> was followed by analysis of antioxidant activity by means of DPPH (2, 2-diphenyl-1-picrylhydrazyl) radical scavenging activity. <em>In vitro</em> anti‐inflammatory activity was evaluated using lipoxygenase inhibition, albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity at different concentrations. Aspirin was used as a standard drug for the study of anti‐inflammatory activity. Linear regression analysis was used to calculate half maximal inhibitory concentration, IC50 value. The zone of inhibition was performed against common pathogens to determine the antimicrobial activity at different concentrations of plant extracts (60%, 70%, 80%).</p><p><strong>Results: </strong>The phytochemical analysis revealed the presence of carbohydrates, amino acid, alkaloids, tannins, flavonoids, glycosides, xanthoproteins, and phenols. The total phenolic and flavonoid content was found to be 2.25±0.04 mg GAE/g (gallic acid equivalents) and 1.2±0.05 mg QE/g (Quercetin equivalents) of fresh weight tissue respectively. The IC50 values for hydrogen peroxide scavenging activity were found to be 244.6 µg/ml. The extract inhibited the lipoxygenase enzyme activity with an IC50 value of 356.3 µg/ml. Maximum inhibition of heat-induced protein denaturation of 69% was observed at 400 μg/ml, IC50 249.6 μg/ml. Proteinase activity was also significantly inhibited (IC50 = 421.6 μg/ml). Membrane stabilization assay attributed minor protection by the leaf extract with an IC50 of 206.7. It was observed that <em>E. coli</em> were inhibited at all concentrations, followed by <em>Klebsiella</em> and <em>Pseudomonas</em>.</p><p><strong>Conclusion: </strong>Results indicate that L. aspera possess anti-inflammatory properties due to the strong occurrence of polyphenolic compounds such as alkaloids, flavonoids, tannins and steroids that serve as free radical inhibitors or scavenger. Compounds of the plant L. aspera may hence be used as lead compounds for designing potent anti-inflammatory drug which can be used for treatment of various diseases.</p><p> </p>

scholarly journals In vitro anti-inflammatory activity of mangrove plant Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae)

2018 ◽  
Vol 5 (10) ◽  
pp. 417-426 ◽  
Author(s):  
Samanjit Kaur ◽  
M. Syed Ali ◽  
V. Anuradha ◽  
V. Suganya ◽  
A. Ashashalini ◽  
...  
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Bark Extract ◽  
Root Extract ◽  
Rhizophora Mucronata ◽  
Membrane Stabilization ◽  
In Vivo Models ◽  
Maximum Inhibition ◽  
Anti Inflammatory

In the present study, analysis of in vitro inflammatory showed whole plant of Rhizophora mucronata Lam. (Malpighiales: Rhizophoraceae) can be the potent source. The data from this study showed that the R. mucronata leaf, bark and root extract could serve as an important anti-inflammatory agent. Moreover, among the three extracts, the stilt root and leaves extract showed highest anti inflammatory. In vitro anti-inflammatory activity of the selected plant extracts was evaluated using albumin denaturation, membrane stabilization and proteinase inhibitory assays. As part of the investigation on the mechanism of the anti-inflammation activity, ability of extract protein denaturation was studied. Maximum inhibition (296.26%) was observed from root extract followed by bark (259.48%) and leaf (237.62%). The extracts inhibited the heat induced hemolysis of RBCs to varying degree as show in table below. The maximum inhibition 284.17% was observed from bark extract followed by root (265.05%) and leaf (232.61%). It reveals that these phytochemical constituents are responsible to maximum protection of protein denaturation, albumin denaturation and membrane stabilization assay. The future work will be determination of anti-inflammatory and anti-arthritic activities by in vivo models.

HYDRO-ALCOHOLIC EXTRACT OF THEVETIA PERUVIANA ATTENUATES ACUTE AND SUB-ACUTE INFLAMMATION: AN IN VITRO STUDY

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (06) ◽  
pp. 33-37
Author(s):  
N. K Choudhary ◽  
◽  
V Sharma ◽  
S Goyal ◽  
M. S Panwar ◽  
...  
Keyword(s):  
Acute Inflammation ◽  
Protein Denaturation ◽  
In Vitro Study ◽  
In Vitro Models ◽  
Vitro Study ◽  
Inflammatory Activity ◽  
Alcoholic Extract ◽  
Thevetia Peruviana ◽  
Anti Inflammatory

The main aim of the study was to carry out anti-inflammatory activity of Thevetia peruviana in acute and sub acute in vitro models. The hydro-alcoholic extract showed the presence of carbohydrate, tannins, flavonoids, terpenes, steroids, glycosides and alkaloids. Against protein denaturation, the model extract showed 85% inhibition at a concentration of 100μg/mL. Similarly the extract showed 70.55% protection of RBC haemolysis as compared with 74.78% produced by indomethacin.

scholarly journals PHYTOCHEMICAL ANALYSIS AND SUPPRESSION OF INFLAMMATORY TARGETS BY ADATHODA VASICA

2018 ◽  
Vol 11 (5) ◽  
pp. 162
Author(s):  
Amala Reddy ◽  
Sujatha Sundaresan
Keyword(s):  
Methanolic Extract ◽  
Maximum Yield ◽  
Foot Ulcer ◽  
Significant Inhibition ◽  
Inflammatory Activity ◽  
Phytochemical Analysis ◽  
Membrane Stabilization ◽  
Standard Drug ◽  
Anti Inflammatory

 Objectives: A persistent and chronic inflammation may act as an underlying definition for many of the long-term illness such as diabetic foot ulcer, Alzheimer’s disease and rheumatoid arthritis. Folklore medicinal plants provide immense health benefits with new remedies. Active compounds from plants containing alkaloids and phenolic compounds (flavonoids and tannins) possess antioxidant and anti-inflammatory activity. The leaves of Adhatoda vasica are most known for its effectiveness in treating respiratory diseases such as asthma, chronic bronchitis, and other respiratory conditions. In this report, we investigated the possible action of A. vasica against inflammation.Methods: Solvents with varying polarity (Hexane, dichloromethane, ethyl acetate, and methanol) were used for the sequential extraction of leaves of A. vasica Linn (Family: Acanthaceae). The anti‐inflammatory activity was assessed using simple in vitro methods. Phenols, flavonoids, phytosterols, saponins, alkaloids, and tannins were analyzed qualitatively using standard protocols. Anti‐inflammatory activity was evaluated using albumin denaturation assay, membrane stabilization assay and proteinase inhibitory activity. Aspirin (diclofenac) was used as a standard drug for the study of anti-inflammatory activity.Results: Our results substantiated that methanolic extract of A. vasica gave a maximum yield of 12.5% and apparently all bioactive phytochemical constituents were at a higher concentration in the methanolic extract. Methanolic extract of A. vasica showed a significant inhibition of the heat-induced albumin denaturation, red blood cells membrane stabilization, and proteinase inhibitory effects with 86±1.46, 88±1.24, and 96±0.93% for 1000 μg/ml, respectively.Conclusion: The above results delineate that the bioactive components were extracted using high polar solvent are mostly flavonoids and related polyphenols. These compounds may be the active constituents to render the anti-inflammatory activity to A. vasica.

scholarly journals STUDY OF PARMELIA PERLATA FOR ITS POTENTIAL AS ANTI-INFLAMMATORY AND ANTIARTHRITIC AGENT USING IN VITRO MODEL

2019 ◽  
Vol 12 (1) ◽  
pp. 95 ◽  
Author(s):  
Yogesh Diwakar ◽  
Chitra V ◽  
Evelyn Sharon S
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Maximum Effect ◽  
Dependent Manner ◽  
Membrane Stabilization ◽  
Human Red Blood Cells ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Vitro Model

Objective: The objective of this study was to evaluate the anti-inflammatory and antiarthritic potential of Parmelia perlata. Methods: The relative study is based on in vitro anti-inflammatory and antiarthritic activity using hydroalcoholic extract of P. perlata (HAEPP). The preliminary phytochemical tests showed the presence of various phytochemical compounds such as alkaloids, flavonoids, and glycosides since the lichen species of P. perlata has the folklore claim of anti-inflammatory activity, thus it was studied by human red blood cells membrane stabilization method, and arthritic activity was carried using protein denaturation method using diclofenac as a standard.Results: The results showed eminent anti-inflammatory and antiarthritic activity in a dose-dependent manner. The membrane stabilization showed the maximum effect at 78.54% at the concentration of 1000 μg/, and the protein denaturation was also found maximum at 1000 μg/ml concentration at 79.43%. Thus, our research states the potent anti-inflammatory activity and antiarthritic effect in P. perlata. Conclusion: The HEAPP has a potent anti-inflammatory activity and antiarthritic activity. A further study has to be conducted to establish the pharmacological evidence behind the compound and the mechanism of action of the HAEPP on the inhibition of the inflammation process.

IN VITRO ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT AND DIFFERENT FRACTIONS OF CENTELLA ASIATICA LEAVES BY PROTEIN DENATURATION

INDIAN DRUGS ◽  
2019 ◽  
Vol 56 (06) ◽  
pp. 86-89
Author(s):  
S Sharma ◽  
◽  
R. Trivedi ◽  
N. K. Choudhary
Keyword(s):  
Petroleum Ether ◽  
Skin Diseases ◽  
Methanolic Extract ◽  
Protein Denaturation ◽  
Diclofenac Sodium ◽  
Centella Asiatica ◽  
Inflammatory Activity ◽  
Standard Drug ◽  
Anti Inflammatory

Inflammation might be a complex organic reaction to a hazardous stimulant such as pathogens, or injured tissues and mainly causes itching, swelling, skin redness, warm and slight pain. Herbal drugs are widespread in India for their effectiveness, easy availability at low cost and provide low toxicity as compared to modern drugs. Centella asiatica is one of the oldest Ayurvedic medicinal plants, used in treatment of various skin diseases. The aim of our present research was to evaluate the in vitro anti-inflammatory activity of methanolic extract and different fractions of C. asiatica leaves. In protein denaturation method, the percentage inhibition for methanolic extract was observed to be 40.22%. The petroleum ether and n-butanol fraction of methanolic extract of C. asiatica were observed to exhibits 54.12 and 44.42% inhibition, respectively. Diclofenac sodium was used as a standard drug. In comparison with other fractions petroleum ether and n-butanol fractions showed best activity. The preliminary phytochemical studies of n-butanol fractions and n-butanol fractions showed the presence of terpenoids, flavonoids etc., which are used in the treatment of inflammation. Thus, we can call the latter as intense anti-inflammatory agent.

scholarly journals STUDY OF PARMELIA PERLATA FOR ITS POTENTIAL AS ANTI-INFLAMMATORY AND ANTIARTHRITIC AGENT USING IN VITRO MODEL

2019 ◽  
Vol 12 (1) ◽  
pp. 95
Author(s):  
Yogesh Diwakar ◽  
Chitra V ◽  
Evelyn Sharon S
Keyword(s):  
Protein Denaturation ◽  
Inflammatory Activity ◽  
Maximum Effect ◽  
Dependent Manner ◽  
Membrane Stabilization ◽  
Human Red Blood Cells ◽  
Phytochemical Compounds ◽  
Anti Inflammatory ◽  
Vitro Model

Objective: The objective of this study was to evaluate the anti-inflammatory and antiarthritic potential of Parmelia perlata. Methods: The relative study is based on in vitro anti-inflammatory and antiarthritic activity using hydroalcoholic extract of P. perlata (HAEPP). The preliminary phytochemical tests showed the presence of various phytochemical compounds such as alkaloids, flavonoids, and glycosides since the lichen species of P. perlata has the folklore claim of anti-inflammatory activity, thus it was studied by human red blood cells membrane stabilization method, and arthritic activity was carried using protein denaturation method using diclofenac as a standard.Results: The results showed eminent anti-inflammatory and antiarthritic activity in a dose-dependent manner. The membrane stabilization showed the maximum effect at 78.54% at the concentration of 1000 μg/, and the protein denaturation was also found maximum at 1000 μg/ml concentration at 79.43%. Thus, our research states the potent anti-inflammatory activity and antiarthritic effect in P. perlata. Conclusion: The HEAPP has a potent anti-inflammatory activity and antiarthritic activity. A further study has to be conducted to establish the pharmacological evidence behind the compound and the mechanism of action of the HAEPP on the inhibition of the inflammation process.

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