scholarly journals Identification and Phylogenetic Analyses of Two Isoforms of the Antibacterial Gene Diptericin from the Larval Tissue of Musca domestica (Diptera: Muscidae)

2017 ◽  
Vol 39 (1) ◽  
pp. 24-31
Author(s):  
AlaaEddeen M. Seufi ◽  
Abada A. Assar ◽  
Magda M. Aboelmahasen ◽  
Shaymaa H. Mahmoud
2009 ◽  
Vol 76 (4) ◽  
pp. 994-998 ◽  
Author(s):  
Pannipa Prompiboon ◽  
Verena-Ulrike Lietze ◽  
John S. S. Denton ◽  
Christopher J. Geden ◽  
Tove Steenberg ◽  
...  

ABSTRACT The housefly, Musca domestica, is a cosmopolitan pest of livestock and poultry and is of economic, veterinary, and public health importance. Populations of M. domestica are naturally infected with M. domestica salivary gland hypertrophy virus (MdSGHV), a nonoccluded double-stranded DNA virus that inhibits egg production in infected females and is characterized by salivary gland hypertrophy (SGH) symptoms. MdSGHV has been detected in housefly samples from North America, Europe, Asia, the Caribbean, and the southwestern Pacific. In this study, houseflies were collected from various locations and dissected to observe SGH symptoms, and infected gland pairs were collected for MdSGHV isolation and amplification in laboratory-reared houseflies. Differences among the MdSGHV isolates were examined by using molecular and bioassay approaches. Approximately 600-bp nucleotide sequences from each of five open reading frames having homology to genes encoding DNA polymerase and partial homology to the genes encoding four per os infectivity factor proteins (p74, pif-1, pif-2, and pif-3) were selected for phylogenetic analyses. Nucleotide sequences from 16 different geographic isolates were highly homologous, and the polymorphism detected was correlated with geographic source. The virulence of the geographic MdSGHV isolates was evaluated by per os treatment of newly emerged and 24-h-old houseflies with homogenates of infected salivary glands. In all cases, 24-h-old flies displayed a resistance to oral infection that was significantly greater than that displayed by newly eclosed adults. Regardless of the MdSGHV isolate tested, all susceptible insects displayed similar degrees of SGH and complete suppression of oogenesis.


2019 ◽  
Vol 10 (2) ◽  
pp. 44-50
Author(s):  
Rinaldi Daswito ◽  
Rima Folentia ◽  
M Yusuf MF

One of the diseases that can be transmitted by flies is diarrhea. Green betel leaf contains essential oils, chavicol, arecoline, phenol, and tannins which function as plant-based insecticides. This study aimed to determine the effectiveness of green betel leaf extract (Piper betel) as a plant-based insecticide on the number of mortality of house flies (Musca domestica). The research was an experimental study used After Only Design used the One Way Anova test with a 95% confidence level. The samples used were 360 ​​house flies. Each treatment of 30 house flies with 4 repetitions and used three concentrations of green betel leaf extract (25%, 50%, 75%). The study was conducted at the Chemistry and Microbiology Laboratory of Health Polytechnic Tanjungpinang, while the location of the fly collection was at the Tokojo Garbage Collection Station in Bintan Regency. The number of mortality of house flies at a concentration of 25% was 81 heads (67.5%), 50% concentrations were 93 heads (77.5%), and at a concentration of 75% were 103 heads (85.83%). There was an effect of green betel leaf extract on the mortality of house flies (p-value 0.0001 <0.05) with the most effective concentration of 75%. Further research is needed to obtain a finished product utilizing green betel leaf extract as a vegetable insecticide, especially in controlling the fly vector. Need further research on the use of green betel leaf extract as a vegetable insecticide controlling the fly vector by taking into account the amount of spraying and the age of the fly.   Keywords: Green betel leaf extract , organic insecticide, houseflies


2020 ◽  
Vol 142 ◽  
pp. 83-97
Author(s):  
A Chandran ◽  
PU Zacharia ◽  
TV Sathianandan ◽  
NK Sanil

The present study describes a new species of myxosporean, Ellipsomyxa ariusi sp. nov., infecting the gallbladder of the threadfin sea catfish Arius arius (Hamilton, 1822). E. ariusi sp. nov. is characterized by bivalvular, ellipsoid or elongate-oval myxospores with smooth spore valves and a straight suture, arranged at an angle to the longitudinal spore axis. Mature myxospores measured 10.1 ± 0.8 µm in length, 6.8 ± 0.5 µm in width and 7.7 ± 0.7 µm in thickness. Polar capsules are equal in size and oval to pyriform in shape. They are positioned at an angle to the longitudinal myxospore axis and open in opposite directions. Polar capsules measured 2.8 ± 0.3 µm in length and 2.5 ± 0.4 µm in width; polar filaments formed 4-5 coils, and extended to 32.2 ± 2.1 µm in length. Monosporic and disporic plasmodial stages attached to the wall of gallbladder. Molecular analysis of the type specimen generated a 1703 bp partial SSU rDNA sequence (MN892546), which was identical to the isolates from 3 other locations. In phylogenetic analyses, genus Ellipsomyxa appeared monophyletic and E. ariusi sp. nov. occupied an independent position in maximum likelihood and Bayesian inference trees with high bootstrap values. The overall prevalence of infection was 54.8% and multiway ANOVA revealed that it varied significantly with location, year, season, sex and size of the fish host. Histopathological changes associated with E. ariusi sp. nov. infection included swelling, vacuolation and detachment of epithelial layer, reduced mucus production and altered consistency and colour of bile. Based on the morphologic, morphometric and molecular differences with known species of Ellipsomyxa, and considering differences in host and geographic locations, the present species is treated as new and the name Ellipsomyxa ariusi sp. nov. is proposed.


2020 ◽  
pp. 28-30
Author(s):  
Mikhail A. Levchenko ◽  

The control of houseflies (Musca domestica L.) in veterinary surveillance premises is an important measure for the welfare of animals against infectious and invasive diseases. For this purpose, the most effective chemical insecticides are used. To prevent possible resistance to them, bait insecticides with two active binary ingredients from different chemical classes are used. The work was carried out in the laboratory of the Tyumen Scientific Center and in the production conditions of ZAO Pyshminskaya Poultry Farm. This material presents the main stages in the development of a prototype of the insecticidal bait Mukhnet AX containing two insecticides: 1.5% acetamiprid and 6% chlorfenapir. For this purpose, adults of houseflies Musca domestica L. 3-5 days old were used. Under laboratory conditions, effective rational doses of the above insecticides were determined by group feeding, feeding and by the method of assessing food insecticidal baits when fighting flies from 0.00002 to 4% concentrations. According to the results of laboratory studies, it was found that the optimal doses causing 100% death of insects were 0.5% for acetamiprid and 2% for chlorfenapir. The insecticidal efficiency of the developed bait Mukhnet AH against flies in production conditions on the first day after the treatment of the livestock building was 91.25%. The insecticidal effect of the measures taken lasted for at least 6 days. The restoration of the number to the previous level of the number of insects occurred after 10 days. Based on the results obtained, the Method of using the composition of an insecticidal bait agent in the fight against Musca domestica was proposed and patented.


2019 ◽  
Vol 44 (4) ◽  
pp. 930-942
Author(s):  
Geraldine A. Allen ◽  
Luc Brouillet ◽  
John C. Semple ◽  
Heidi J. Guest ◽  
Robert Underhill

Abstract—Doellingeria and Eucephalus form the earliest-diverging clade of the North American Astereae lineage. Phylogenetic analyses of both nuclear and plastid sequence data show that the Doellingeria-Eucephalus clade consists of two main subclades that differ from current circumscriptions of the two genera. Doellingeria is the sister group to E. elegans, and the Doellingeria + E. elegans subclade in turn is sister to the subclade containing all remaining species of Eucephalus. In the plastid phylogeny, the two subclades are deeply divergent, a pattern that is consistent with an ancient hybridization event involving ancestral species of the Doellingeria-Eucephalus clade and an ancestral taxon of a related North American or South American group. Divergence of the two Doellingeria-Eucephalus subclades may have occurred in association with northward migration from South American ancestors. We combine these two genera under the older of the two names, Doellingeria, and propose 12 new combinations (10 species and two varieties) for all species of Eucephalus.


2018 ◽  
Author(s):  
Alex Mesoudi

Cultural evolution is a branch of the evolutionary sciences which assumes that (i) human cognition and behaviour is shaped not only by genetic inheritance, but also cultural inheritance (also known as social learning), and (ii) this cultural inheritance constitutes a Darwinian evolutionary system that can be analysed and studied using tools borrowed from evolutionary biology. In this chapter I explore the numerous compatibilities between the fields of cultural evolution and cultural psychology, and the potential mutual benefits from their closer alignment. First, understanding the evolutionary context within which human psychology emerged gives added significance to the findings of cultural psychologists, which reinforce the conclusion reached by cultural evolution scholars that humans inhabit a ‘cultural niche’ within which the major means of adaptation to difference environments is cultural, rather than genetic. Hence, we should not be surprised that human psychology shows substantial cross-cultural variation. Second, a focus on cultural transmission pathways, drawing on cultural evolution models and empirical research, can help to explain to the maintenance of, and potential changes in, cultural variation in psychological processes. Evidence from migrants, in particular, points to a mix of vertical, oblique and horizontal cultural transmission that can explain the differential stability of different cultural dimensions. Third, cultural evolutionary methods offer powerful means of testing historical (“macro-evolutionary”) hypotheses put forward by cultural psychologists for the origin of psychological differences. Explanations in terms of means of subsistence, rates of environmental change or pathogen prevalence can be tested using quantitative models and phylogenetic analyses that can be used to reconstruct cultural lineages. Evolutionary considerations also point to potential problems with current cross-country comparisons conducted within cultural psychology, such as the non-independence of data points due to shared cultural history. Finally, I argue that cultural psychology can play a central role in a synthetic evolutionary science of culture, providing valuable links between individual-oriented disciplines such as experimental psychology and neuroscience on the one hand, and society-oriented disciplines such as anthropology, history and sociology on the other, all within an evolutionary framework that provides links to the biological sciences.


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