This study was carried out to determine the effect of growing conditions underdifferent altitudes on seed production in different potato crosses in Tokat Provinceof Turkey in 2015. Plants were grown and crosses were made in open field or nethouse in Tokat location (altitude 600 m asl.), open field or net house in Artovalocation (altitude 1200 m asl.), or controlled polycarbonate greenhouse. Fifteendifferent crosses were made between 12 parent cultivars. Production of hybridpotato seed was considered “crossing success”. Results showed that parentcultivars and cross combinations affected crossing success and no seeds wereproduced from some combinations while some combinations produced abundantseeds. The highest number of seeds were produced in controlled polycarbonategreenhouse, whereas very low amount of seeds were produced under low altitude(600 m asl.) open field conditions. Polycarbonate greenhouse produced 47.3% ofall seeds, while low altitude net house produced 19.8%, high altitude open fieldproduced 15.8%, high altitude net house produced 13.6% and low altitude openfield produced 3.5%. It was concluded that for a high crossing success, potatocrossing should be made by controlled polycarbonate greenhouse conditions and 23C0 day / 17 C0 night temperatures produced more hybrid seeds.