Development of immunofluorescent diagnostics for the determination of IgM and IgG antibodies to herpes simplex virus types 1 and 2

2021 ◽  
Vol 66 (5) ◽  
pp. 285-290
Author(s):  
N. N. Shershneva ◽  
S. S. Mardanly ◽  
I. N. Klenyaev ◽  
P. V. Samosadova
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Enzyme Linked Immunosorbent Assay ◽  
Central Nervous System Damage ◽  
Clinical Diagnoses ◽  
Simplex Virus ◽  
High Degree ◽  
Cytomegalovirus Infections ◽  
Hsv 1

The Russian kits «HSV-1-Fluorogen-screen» and «HSV-2-Fluorogen-screen» have been developed for the determination of antibodies M and G to herpes simplex virus types 1 and 2 by the immunofluorescence reaction. The kits were used to examine the positive and negative standard «EKOlab» panels sera and showed 100% sensitivity and specificity of the developed tests. 125 samples of blood serum from people with clinical diagnoses such as herpetic, cytomegalovirus infections, pyelonephritis, conjunctivitis and central nervous system damage were tested in parallel with using the enzyme-linked immunosorbent assay systems from different manufacturers and the developed tests «HSV-1-Fluorogen-screen» and «HSV-2-Fluorogen-screen». A high degree of matching of results with comparison sets was observed in examined samples. The developed diagnostics can be successfully used in clinical practice both for screening and for verification of results of the diagnosis of herpesvirus infections caused by herpes simplex virus types 1 and 2.

scholarly journals Limits in Reliability of Glycoprotein G-Based Type-Specific Serologic Assays for Herpes Simplex Virus Types 1 and 2

1999 ◽  
Vol 37 (2) ◽  
pp. 376-379 ◽  
Author(s):  
D. Scott Schmid ◽  
Denise R. Brown ◽  
Rosane Nisenbaum ◽  
Rae Lyn Burke ◽  
D’Anna Alexander ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Western Blot ◽  
Validation Studies ◽  
Enzyme Linked Immunosorbent Assay ◽  
Western Blot Assay ◽  
Cross Sectional ◽  
Glycoprotein G ◽  
Simplex Virus ◽  
Hsv 1

Type-specific serologic assays for herpes simplex virus (HSV) types 1 and 2 based on glycoprotein G-1 (gG-1) (HSV-1) and gG-2 (HSV-2) discriminate between antibodies against HSV-1 and HSV-2. We previously developed a Western blot assay using gG-1 and gG-2 expressed in baculovirus, performed extensive validation studies, and determined that it was both sensitive and specific for type-specific detection of HSV antibody. Here we report that, among a cohort of Thai military recruits, the serostatus of some individuals changed from positive to negative over time (6.6% among those ever positive for HSV-1, and 14.9% among those ever positive for HSV-2). We tested a subset of these specimens in three other gG-based assays: an enzyme-linked immunosorbent assay, an immunoblot strip assay, and a Western blot assay. Positive-to-negative shifts occurred in every assay; the frequency of the shifts ranged from 6.1% to 21.2% of the specimen sets tested. There was only limited agreement among the assays concerning which individuals lost reactivity. This inaccuracy, exhibited by all of the assay protocols, was not predicted by validation studies employing specimens from cross-sectional studies and was most pronounced in HSV-2 testing. This argues for the inclusion of serial blood specimens in serologic assay validation procedures.

Seroprevalence of herpes simplex virus types 1 and 2 and correlates of infection in Jordan

2022 ◽  
pp. 095646242110601
Author(s):  
Samer F Swedan ◽  
Alia’ Darabseh
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Population Estimate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Cross Sectional ◽  
Simplex Virus ◽  
Hsv 1

Background Herpes infections are common infections among populations. Herein, a cross-sectional study was used to determine the seroprevalence of herpes simplex virus type 1 (HSV-1) and herpes simplex virus type 2 (HSV-2) IgG antibodies and their association with potential infection risk factors among Jordanians. Methods A total of 759 serum samples were collected (January to February 2020) and analyzed for HSV-1 and HSV-2 IgG antibodies by enzyme-linked immunosorbent assay. Estimates for population seropositivity were determined by weighting the age-specific seroprevalence by the size of the population in each age stratum. Results The population estimate for HSV-1 seroprevalence was 75.3%. After adjustment for possible confounders, regression analysis revealed higher seroprevalence with increase in age ( p < 0.005) and low household income ( p = 0.002). The population estimate for HSV-2 seroprevalence was 2.9%. No significant differences in HSV-2 seroprevalence were observed in association with age, gender, family size, educational level, and socioeconomic status, likely due to low seropositivity. Conclusions Jordanians have high HSV-1 and low HSV-2 seroprevalence. Periodical studies might be needed to evaluate changes in HSV-1 and HSV-2 seroprevalence over time. This study provides essential epidemiological data for Jordan and the Middle East and North Africa region.

scholarly journals Performance of Two Commercial Glycoprotein G-Based Enzyme Immunoassays for Detecting Antibodies to Herpes Simplex Viruses 1 and 2 in Children and Young Adolescents

2002 ◽  
Vol 9 (5) ◽  
pp. 1124-1125 ◽  
Author(s):  
Charles T. Leach ◽  
Rhoda L. Ashley ◽  
Jacques Baillargeon ◽  
Hal B. Jenson
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Enzyme Linked Immunosorbent Assay ◽  
Aged Patients ◽  
Herpes Simplex Viruses ◽  
Glycoprotein G ◽  
Children And Young Adolescents ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT In 61 patients 1 to 14 years of age, the Gull/Meridian enzyme-linked immunosorbent assay (ELISA) had a sensitivity of 100% for herpes simplex virus type 1 (HSV-1) and specificities of 74% for HSV-1 and 48% for HSV-2. In 128 similarly aged patients, the HerpeSelect ELISA (Focus Technologies) showed sensitivities of 80% for HSV-1 and 88% for HSV-2, and specificities of 97% for HSV-1 and 100% for HSV-2.

scholarly journals Role of Genotypic Analysis of the Thymidine Kinase Gene of Herpes Simplex Virus for Determination of Neurovirulence and Resistance to Acyclovir

1999 ◽  
Vol 37 (10) ◽  
pp. 3171-3174 ◽  
Author(s):  
N. Y. Lee ◽  
Y.-W. Tang ◽  
M. J. Espy ◽  
C. P. Kolbert ◽  
P. N. Rys ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Amino Acid ◽  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Kinase Gene ◽  
Genotypic Analysis ◽  
Simplex Virus ◽  
Hsv 1

Mutations in the thymidine kinase (TK) gene of herpes simplex virus (HSV) have been associated with resistance to acyclovir (ACY) and possible recognition of neurotropic strains. We sequenced a 335-bp segment of the TK gene to determine the frequency of mutations in HSV strains recovered from dermal, genital, and cerebrospinal fluid (CSF) specimens (n = 200; 102 HSV type 1 [HSV-1] 98 HSV-2 strains). Four polymorphic sites were detected in HSV-1 strains; C513T, A528G, C575T, and C672T. Among the polymorphisms, only C575T resulted in a change of amino acid sequence (residue 192, Ala→Val). For HSV-2 strains, only one polymorphism (G420T) which resulted in an amino acid substitution (residue 139, Leu→Phe) was detected. Phenotypic determination of resistance to ACY by a plaque reduction assay of 48 HSV isolates was not correlated with the sequence results of 11 strains in that 7 of these with genotypic polymorphisms were susceptible to the drug in vitro. In addition, of 32 ACY-resistant HSV strains, 28 (87.5%) had no polymorphisms detected in the 335-bp amplicon of the TK gene. There was no statistical difference in the frequency of polymorphisms according to the source of the specimens. We conclude that the detection of nucleic acid polymorphisms in a previously implicated 335-bp segment of the TK gene cannot be interpreted as indicative of either ACY resistance or neurotropism of HSV strains from dermal, genital, and CSF sources.

scholarly journals Seroprevalence and characterisation of herpes simplex virus from human immunodeficiency virus in samples collected from two provinces in South Africa: a retrospective study

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 105
Author(s):  
Oluwafemi Samuel Obisesan ◽  
Nomathamsanqa Patricia Sithebe ◽  
Hazel Tumelo Mufhandu
Keyword(s):  
South Africa ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Goodness Of Fit ◽  
High Rate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Goodness Of Fit Test ◽  
Simplex Virus ◽  
Hiv 1 ◽  
Hsv 1

Background: Herpes simplex virus (HSV) is a widely distributed human pathogen that is known for its ulcerative lesions at the infection site. HSV can cause persistent infection in the host that is often followed by a period of latency within the neurons. Considering the high rate of HIV infection in South Africa, it is important to assess the seroprevalence of HSV with a focus to determine the epidemiological association between HSV-DNA and HIV-1 in the population. Methods: A total of 44 sera samples were screened for HSV and HIV-1 using the highly sensitive enzyme-linked immunosorbent assay (ELISA). The ELISA positive samples were characterized using polymerase chain reaction (PCR) to confirm the positivity of both viruses and to further differentiate HSV into HSV-1 and -2. Thereafter, the samples were analysed for relatedness using phylogenetic analysis. Results: Of the 44 samples, 36 (81.8%) were positive for HIV-1, while 35 (79.5%) were positive for HSV when screened with ELISA kits. The PCR results, with the use of type specific primers, showed that 4/35 (11.4%) samples were specific for HSV-1 while 30/35 (85.7%) were specific for HSV-2. Statistical analysis performed using the chi-squared goodness-of-fit test showed that there is a significant relationship between HSV-2 and HIV-1 transmission. Conclusions: The prevalence of HSV in the population is high with an increased HSV-2 infection in women. Our study shows that some of the HSV-2 isolates are not related to the clinical isolate SD90e from South Africa, suggesting diversity in HSV-2 viral transmission.

Clinical Manifestations of Primary Herpes Simplex Virus Type 1 Infection in a Closed Community

PEDIATRICS ◽  
1991 ◽  
Vol 87 (2) ◽  
pp. 152-158
Author(s):  
Kiyotaka Kuzushima ◽  
Hiroshi Kimura ◽  
Shinji Kido ◽  
Naoki Hanada ◽  
Motohiro Shibata ◽  
...  
Keyword(s):  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Virus Type ◽  
Herpes Simplex Virus Type ◽  
Enzyme Linked Immunosorbent Assay ◽  
Herpetic Gingivostomatitis ◽  
Simplex Virus ◽  
Observation Period ◽  
Hsv 1

The clinical features and the molecular epidemiology of primary herpes simplex virus type 1 (HSV-1) infection among children younger than 3 years of age were investigated in day-care nursery. Serial sera were assayed for anti-HSV-1 glycoprotein B antibody by enzyme-linked immunosorbent assay. Serologic examinations revealed 55 cases of primary HSV infection during the observation period. Fifty-one of them (93%) had typical herpetic gingivostomatitis, showing a high rate of clinically overt infection. Four outbreaks of herpetic gingivostomatitis were observed during the observation period. Forty-one children were infected with HSV-1 in the outbreaks. The rates of infection in the susceptible children were 81%, 73%, 78%, and 100%, respectively, in the four outbreaks. Restriction endonuclease analysis of DNA of isolated HSV revealed that only one strain of HSV-1 had been transmitted among children for a long period.

scholarly journals Seroprevalence and characterisation of herpes simplex virus from human immunodeficiency virus in samples collected from two provinces in South Africa: a retrospective study

F1000Research ◽  
2021 ◽  
Vol 10 ◽  
pp. 105
Author(s):  
Oluwafemi Samuel Obisesan ◽  
Nomathamsanqa Patricia Sithebe ◽  
Hazel Tumelo Mufhandu
Keyword(s):  
South Africa ◽  
Herpes Simplex Virus ◽  
Herpes Simplex ◽  
Goodness Of Fit ◽  
High Rate ◽  
Enzyme Linked Immunosorbent Assay ◽  
Goodness Of Fit Test ◽  
Simplex Virus ◽  
Hiv 1 ◽  
Hsv 1

Background: Herpes simplex virus (HSV) is a widely distributed human pathogen that is known for its ulcerative lesions at the infection site. HSV can cause persistent infection in the host that is often followed by a period of latency within the neurons. Considering the high rate of HIV infection in South Africa, it is important to assess the seroprevalence of HSV with a focus to determine the epidemiological association between HSV-DNA and HIV-1 in the population. Methods: A total of 44 sera samples were screened for HSV and HIV-1 using the highly sensitive enzyme-linked immunosorbent assay (ELISA). The ELISA positive samples were characterized using polymerase chain reaction (PCR) to confirm the positivity of both viruses and to further differentiate HSV into HSV-1 and -2. Thereafter, the samples were analysed for relatedness using phylogenetic analysis. Results: Of the 44 samples, 36 (81.8%) were positive for HIV-1, while 35 (79.5%) were positive for HSV when screened with ELISA kits. The PCR results, with the use of type specific primers, showed that 4/35 (11.4%) samples were specific for HSV-1 while 30/35 (85.7%) were specific for HSV-2. Statistical analysis performed using the chi-squared goodness-of-fit test showed that there is a significant relationship between HSV-2 and HIV-1 transmission. Conclusions: There is a significant positive association between HSV-2 and HIV-1 in the study population. Our study shows that some of the HSV-2 isolates are not related to the clinical isolate SD90e from South Africa, suggesting diversity in HSV-2 viral transmission.

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