scholarly journals Seasonal variation of the effect of extremely diluted agitated gibberellic acid (10-30) on wheat seedling development

2021 ◽  
Vol 10 (35) ◽  
pp. 79-79
Author(s):  
Waltraud Scherer-Pongratz ◽  
Peter Christian Endler
Keyword(s):  
Gibberellic Acid ◽  
Wheat Seedling ◽  
Seedling Development ◽  
Control Groups ◽  
Wheat Growth ◽  
Autumn Season ◽  
Reliable Effect ◽  
Different Seasons ◽  
Stalk Length ◽  
Bio Assay

Objective: Performing a study on a wheat growth bio assay with a homeopathic dilution of gibberellic acid at different seasons of the year. Methods: Grains of winter wheat (Triticum aestivum, Capo variety) were observed under the influence of extremely diluted gibberellic acid (10-30, 30x). Analogously prepared water was used for control. 15 experiments were performed, 9 in autumn season (5 researchers, 4,440 grains per group), and 6 in winter / spring (4 researchers, with 3,140 grains per group). Results: All 9 autumn experiments showed less stalk growth in the verum group (p > 0.01 in 4 cases, p > 0.05 in 3, trend in 2 cases). Mean stalk lengths (mm) were 46.97 + 20.50 for verum and 50.66 + 19.77 for control at grain level (N = 4,440 per group) and + 3.87 and + 3.38 respectively at dish level (217 cohorts of 20 or 25 grains per treatment group). Verum stalk length (92.72%) was 7.28% smaller than control stalk length (100%). In contrast, no reliable effect was found in experiments performed in winter / spring (less stalk growth in 1 case, no difference in 1, more growth in 3 cases). Overall verum stalk length (103.64%) was 3.64% slightly greater than control stalk length (100%). Data were found to be homogeneous within the control groups as well as within the verum groups. Conclusion: Results suggest that especially in the experiments performed in autumn, there was an influence of gibberellic acid 30x on wheat seedling development. The effect size is small when calculation is done on the basis of grains (d = 0.18) but high when done on the basis of dishes (d = 1.02). In contrast, no reliable effect was found in experiments performed in winter / spring. Further experiments should thus be performed in the autumn season.

scholarly journals Seasonal variation of the effect of extremely diluted agitated gibberellic acid (10-30) on wheat stalk growth - a multi researcher study

2021 ◽  
Vol 10 (36) ◽  
pp. 263-264
Author(s):  
Peter Christian Endler ◽  
Christian Reich ◽  
Wolfgang Matzer ◽  
Thomas Reischl ◽  
Anna Maria Hartmann ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Seasonal Variation ◽  
Gibberellic Acid ◽  
Effect Size ◽  
High Dilution ◽  
Reliable Effect ◽  
Different Seasons ◽  
Stalk Length ◽  
Bio Assay

Control experiments were performed at different seasons of the year as a follow-up to pilot experiments [1] where a homeopathic high dilution of gibberellic acid had influenced growth in a wheat bio assay (7 days). Grains of winter wheat (Triticum aestivum, Capo variety) were observed under the influence of extremely diluted gibberellic acid (10-30) prepared by stepwise dilution and agitation according to a protocol derived from homeopathy (“G30x”). Analogously prepared water was used for control (“W30x”). Following up on 5 pilot experiments (4 in autumn 2007, 1 in spring 2008), 10 experiments were performed (5 in autumn 2008 or 2009 and 5 in winter 2009 or 2010) with a total of 9 experiments in autumn season (5 researchers, about 9,000 grains), and 6 in winter/spring (4 researchers, about 6,000 grains). Germination rates after 7 days were slightly higher for the autumn experiments (96.1%) than for the winter/spring experiments (94.8%) (p > 0,05), with a non significant trend of more seedlings having germinated in the verum group in the autumn experiments (p > 0,05). All of the 9 autumn experiments (i.e. pilot as well as repetition experiments) showed less stalk growth in the verum group (statistically significant with p < 0.01 in 4, with p < 0.05 in 3 cases, trend in 2 cases). Mean stalk lengths (mm) were 46.97 + 20.50 for the verum group and 50.66 + 19.77 for control (mean + S.D.) at grain level (N = 4,440 per group) and + 3.87 and + 3.38 (+ S.D.) respectively at dish level (217 cohorts of 20 or 25 grains per treatment group). In other words, verum stalk length (92.72%) was 7.28% smaller than control stalk length (100%). The effect size (D means : S.D.), calculated on the basis of dishes, was high (d = 1.02). In contrast, no reliable effect was found in experiments performed in winter/spring (less stalk growth in the verum group in one case, no difference in 2 cases, and more growth in 3 cases). Overall verum stalk length (103.64%) was slightly greater than control stalk length (100%). The effect size, however, was small (d = 0.45). The new data are in line with the 2007 findings, i.e. confirm that gibberellic acid 30x does influence stalk growth.

scholarly journals Seasonal Variation of the Effect of Extremely Diluted Agitated Gibberellic Acid (10e-30) on Wheat Stalk Growth: A Multiresearcher Study

2011 ◽  
Vol 11 ◽  
pp. 1667-1678 ◽  
Author(s):  
Peter Christian Endler ◽  
Wolfgang Matzer ◽  
Christian Reich ◽  
Thomas Reischl ◽  
Anna Maria Hartmann ◽  
...  
Keyword(s):  
Seasonal Variation ◽  
Gibberellic Acid ◽  
High Dilution ◽  
Wheat Growth ◽  
Autumn Season ◽  
Different Seasons

The influence of a homeopathic high dilution of gibberellic acid on wheat growth was studied at different seasons of the year. Seedlings were allowed to develop under standardized conditions for 7 days; plants were harvested and stalk lengths were measured. The data obtained confirm previous findings, that ultrahigh diluted potentized gibberellic acid affects stalk growth. Furthermore, the outcome of the study suggests that experiments utilizing the bioassay presented should best be performed in autumn season. In winter and spring, respectively, no reliable effects were found.

scholarly journals Pre stimulation by gibberellic acid and the effect of extremely diluted agitated gibberellic acid on wheat stalk growth

2021 ◽  
Vol 11 (40) ◽  
pp. 124-125
Author(s):  
Sonja Hribar-Marko ◽  
Waltraud Scherer-Pongratz ◽  
Harald Lothaller ◽  
Peter Christina Endler
Keyword(s):  
Gibberellic Acid ◽  
Wheat Seedling ◽  
World Journal ◽  
High Dilution ◽  
Wheat Growth ◽  
Slowing Down ◽  
Scientific World ◽  
Pre Treatment ◽  
High Concentrations ◽  
Treatment Concentration

Background: In previous multicentre studies[1,2], the influence of a homeopathic ultra high dilution of gibberellic acid on wheat growth was scrutinized. Data showed that this test dilution slowed down stalk growth when experiments were performed in autumn season. Aim: To test the hypothesis that pre treatment of grains with high concentrations of gibberellic acid will enhance the effect of the ultra high dilution of the plant hormone Methods: Grains of winter wheat (Triticum aestivum) were observed under the influence of extremely diluted gibberellic acid (10-30) prepared by stepwise dilution and agitation according to a protocol derived from homeopathy (“G30x”). Analogously prepared water was used for control (“W30x”). Grains (500 or 1000 per group) were pre treated with (not agitated) gibberellic acid 10-5, 10-4 and 10-3 (“Ge-5, Ge-4, Ge-3”) or with water (“W”) for control prior to treatment with G30x or W30x. Seedlings were allowed to develop under standardized conditions for 7 days; plants were harvested and stalk lengths were measured. Results: With regard to pre treatment, it can be seen that the groups treated with Ge-3 showed most growth, followed by the Ge-4 groups, the Ge-5 groups and the non pre treated W groups. This decline is modulated by the application of G30x and W30x (figure 1). The lower the pre treatment concentration of G, the more marked is a slowing down effect of G30x versus W30x. Conclusion: The hypothesis that pre treatment of grains with high concentrations of gibberellic acid will enhance the effect of G30x had to be rejected. In contrast, G30x slowed down stalk growth in the W group with p < 0.001 but only moderately in the Ge-5 and Ge-4 group and not at all in the Ge-3 group. Keywords: homeopathy, wheat stalk growth, gibberellic acid, high dilution References: [1] Pfleger A., Hofäcker J., Scherer-Pongratz W., Lothaller H., Reich C., Endler P.C. The effect of extremely diluted agitated gibberellic acid (10e-30) on wheat seedling development – a two researcher study. Complementary Therapies in Medicine 2011; 19: 164-169. [2] Endler PC, Matzer W, Reich C, Reischl T, Hartmann AM, Thieves K, Pfleger A, Hofäcker J, Lothaller H, Scherer-Pongratz W. Seasonal variation of the effect of extremely diluted agitated gibberellic acid (10e-30) on wheat stalk growth – a multi researcher study. The Scientific World Journal 2011; 11: 1667-1678.

Monitoring of changes in 5-n-alkylresorcinols during wheat seedling development

2020 ◽  
Vol 0 (0) ◽  
Author(s):  
Elżbieta G. Magnucka ◽  
Małgorzata P. Oksińska ◽  
Stanisław J. Pietr
Keyword(s):  
De Novo ◽  
Wheat Seedling ◽  
Seedling Development ◽  
Wheat Seed ◽  
Short Term ◽  
Wheat Growth ◽  
Main Compound ◽  
Phenolic Lipids ◽  
Resorcinolic Lipids ◽  
Cuticular Layer

AbstractFor seven days of wheat growth, caryopsis remained the main source of 5-n-alkylresorcinols with C19 and/or C21 homolog as a main compound. Shoot contained small amount of these phenolic lipids; their average content was 3.23% of level obtained in the whole seedling. Moreover, 41.38% of resorcinolic lipids of seven-day-old shoot was accumulated in part of leaf covered by coleoptile. Interestingly, a removal of 1.07% of the primary pool of kernel alkylresorcinols by short-term washing (10 s) of wheat seed with acetone before planting decreased their level only in seed of seven-day old seedling. Compared to the respective controls, this treatment did not affect the amount of these lipids in the green part of seedling that proved that de novo synthesis of 5-n-alkylresorcinols takes place in shoots. The very similar homolog profiles of these lipids in four- and seven-day-old shoots turned out to be markedly less diversified than those found in respective seed samples. Compared to the mature wheat caryopsis, the rise in the content of very-long-chain homologs was observed only in the oldest shoot. Their increased accumulation was probably connected with formation of cuticular layer providing the defensive barrier against various phytopathogens.

Crop residue position and interference with wheat seedling development

2000 ◽  
Vol 55 (3-4) ◽  
pp. 175-182 ◽  
Author(s):  
Stewart B Wuest ◽  
Stephan L Albrecht ◽  
Katherine W Skirvin
Keyword(s):  
Crop Residue ◽  
Wheat Seedling ◽  
Seedling Development ◽  
Residue Position

Proximate Composition and Spatio-temporal Heterogeneity of Phytochemicals in Agave sisalana (sisal) adapted in different agro-ecological zones of Punjab, Pakistan

2021 ◽  
Author(s):  
Sobia Shahzad ◽  
Mumtaz Hussain ◽  
Hassan Munir ◽  
Muhammad Arfan
Keyword(s):  
Environmental Conditions ◽  
Proximate Composition ◽  
Block Design ◽  
Temporal Variations ◽  
Proximate Analysis ◽  
Ecological Zones ◽  
Autumn Season ◽  
Spatial Differences ◽  
Different Seasons ◽  
Spatio Temporal

Abstract Exploring extractable phytochemicals from locally adapted sisal plant vegetation vary seasonally at different locations. This study elaborated proximate composition and phytochemical heterogeneity in sisal due to varying environmental conditions analyzed from five districts, i.e., Chakwal, Khushab, Rawalpindi, Faisalabad, and Layyah in Punjab, Pakistan. Extensive surveying and plant sampling across two years 2017-18 and 2018-19, during mid-spring, summer, autumn, and winter seasons were carried out for understanding the seasonal impact on sisal. The present study was designed in a randomized complete block design (RCBD) and analyzed considering seasonal, yearly, and locational impact. The spatial differences in phytochemicals concentration were strongly associated with environmental conditions prevailing in different seasons. Autumn season reflected saponins, tannins, and flavonoids in higher concentrations during 2018-19 while steroids and terpenoids were higher during spring 2018-19. While Spatio-temporal variations in the proximate analysis were more apparent in different samples collected from different districts. Data recorded for the Khushab district and autumn season reflected the higher composition of a proximate analysis and phytochemical contents as compared to other seasons. Overall, the spatial differences in phytochemicals concentration were strongly associated with soils and environmental conditions prevailing in different seasons in selected districts.

scholarly journals RADIATION USE EFFICIENCY OF MAIZE UNDER INFLUENCE OF DIFFERENT LEVELS OF NITROGEN FERTILIZATION AND TWO DIFFERENT SEASONAL CONDITIONS

2018 ◽  
Vol 49 (6) ◽  
Author(s):  
A. A. Mohammed
Keyword(s):  
Nitrogen Fertilization ◽  
Radiation Use Efficiency ◽  
Dry Matter Accumulation ◽  
Maize Hybrids ◽  
Autumn Season ◽  
Positive Performance ◽  
Different Seasons ◽  
Use Efficiency ◽  
Higher Temperature ◽  
Radiation Use

The objective of this study to estimate  the Radiation Use Efficiency  under effect of three levels of nitrogen (N1 = 80 Kg N ha-1,  N2 =120 Kg  N ha-1, and   N3=160 Kg N ha-1) for a set of maize hybrids include (Gloria, Market, Cruze and Draxma) in two different seasons in spring and fall  of 2017 in Qlyasn-Sulaimani. The experiment was conducted using split plot design with three replicates . The results revealed higher intensity of PAR in autumn season than that of spring (15.672 and 10.555)MJm-2d-1  respectively that led to higher RUE in autumn revealing (4.311,3.546, and 4.702)g MJ-1 m-2 under the effect of (N1,N2, and N3) respectively, while the results of the autumn were higher (5.237, 4.681, and 6.019) g MJ-1 m-2  under the same levels of nitrogen fertilization, the highest application of nitrogen was more effective in autumn. The RGR and the rate of total dry matter accumulation were higher in autumn season displaying positive performance of studied  maize hybrids to the higher intercepted of solar energy as well higher temperature.

scholarly journals Mechanism of Heat Stress and their Management Strategies in Wheat

2021 ◽  
Vol 9 (4) ◽  
pp. 8-30
Author(s):  
Ali Ammar ◽  
Keyword(s):  
Quantitative Trait Locus ◽  
Heat Stress ◽  
Plant Development ◽  
Management Strategies ◽  
Wheat Yield ◽  
Cellular Level ◽  
Seedling Development ◽  
Cell Water ◽  
Wheat Growth ◽  
Trait Locus

Cumulative heat with resulting alterations to weather unfavorably disturb plant development, resultant in disastrous damage in wheat output. With one degree increase in temperature, wheat growth is projected to decrease by 6%. Comprehensive summary of morpho-physiological replies to wheat for temperature pressure might assist expressing suitable approaches in temperature pressure wheat yield development. Moreover, penetrating to conceivable managing approaches might elevate output and sustainability of rising wheat. Main conclusions after this review is follows: (1) temperature pressure meaningfully decreases kernel sprouting and seedling development, turgidness of the cell, water use competence of the plant; (2) During cellular level, temperature pressure interrupts cellular purposes over making unnecessary sensitive oxygen types, foremost towards oxidative pressure; (3) main replies to wheat for temperature pressure comprise improvement to senescence of leaf, decrease in photosynthesis, defusing of enzymes of photosynthesis, production of oxidative losses to chloroplasts; (4) temperature pressure too decreases number of grains and size via upsetting ounce setting, translocation of integrates and period and development proportion of grains; (5) actual methods to manage temperature pressure in wheat comprise screening accessible germplasm beneath field hearings and/or retaining marker aided assortment, claim to external protectants for seeds or plants, mapping quantitative trait locus discussing temperature confrontation and breeding; (6) Well combined genetic and agronomical organization choice might improve wheat acceptance for temperature. Though, achievement to apply numerous methods for temperature pressure organization needs better understanding of temperature acceptance topographies, molecular cloning, and description of genes. General achievement to multifaceted plant temperature pressure administration depends on intensive exertions to crop modelers, molecular biologists, and plant physiologists.

scholarly journals In vitro propagation of the endangered orchid Dendrobium chryseum Rolfefrom protocorms culture

2020 ◽  
Vol 19 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Sabitri Maharjan ◽  
Laxmi Sen Thakuri ◽  
Bir Bahadur Thapa ◽  
Shreeti Pradhan ◽  
Krishna K. Pant ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Mass Propagation ◽  
Endangered Orchid ◽  
Immature Seeds ◽  
Indole 3 Acetic Acid

 The immature seeds of Dendrobiumchryseum, asympodial epiphytic orchid with yellow flowers, were cultured in vitro, and the resultant protocorms were used as explants for seedling development. Protocorms were cultured on½ M.S. medium fortified with Kinetin (Kn), 6-Benzylaminopurine (BAP), and Gibberellic Acid (GA3) in three concentrations (0.5mg/l, 1.0mg/land 2.0 mg/l) both alone and supplemented with 5% and 10% coconut water (C.W.). The highest number of shootsofD. chryseum developed on ½ - M.S. medium fortified with 2.0mg/lofKn and10% C.W. and the longest shootsdeveloped on ½ M.S. media fortified with 1.0mg/lGA3, and 10% C.W. The shoot derived from protocorms were placed in ½ M.S. medium fortified with three different rooting hormones, Indole -3- acetic acid (IAA), Indole -3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA) in different concentrations alone as well as with each 1.0mg/l hormone combined with 10% C.W. The most effective of these media was ½ M.S. medium fortified with 1.5 mg/l IAA for rooting as well as for the production of longest roots. The present study could be useful for standardizing the protocol for mass propagation of the endangered orchid Dendrobiumchryseum.

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