scholarly journals The effects of natural mating and artificial insemination using cryopreserved buck semen on reproductive performance in Alpine goats

2018 ◽  
Vol 61 (4) ◽  
pp. 459-461 ◽  
Author(s):  
Dehouegnon Jerry Agossou ◽  
Nazan Koluman

Abstract. This study compared the effects of natural mating and artificial insemination using frozen buck semen on reproductive performance in Alpine goats. Sixty reproductive Alpine goats were grouped according to natural mating (n=30) and artificial insemination (n=30) breeding methods. Oestrus was synchronised in experimental goats using a vaginal sponge impregnated with 20 mg of progestogen FGA (fluorogestone acetate) for 11 days. At the time of sponge insertion, 150 µg of prostaglandin F2α (PGF2α) analogue was injected intramuscularly. Forty-eight hours prior to vaginal sponge withdrawal, 500 IU equine chorionic gonadotropin (eCG) was injected into the animals. At the end of synchronisation protocol, goats were fertilised using frozen semen or mated with a well-performing buck (ratio: 1 male to 5 females). The pregnancy (pregnant/synchronised goats) and mortality rates (P<0.05) were higher (93 % vs. 70 %; 2 % vs. 4 %) in the naturally mated goats than the artificially inseminated group. Kids' live weight at birth was similar (3.83±0.23 and 3.15±0.11 kg) in both groups. The oestrus synchronisation followed by natural mating achieved better reproductive performance than the goats artificially inseminated using frozen semen. However, the artificially inseminated animals displayed an acceptable twinning rate.

2014 ◽  
Vol 54 (6) ◽  
pp. 715 ◽  
Author(s):  
K. G. Geenty ◽  
F. D. Brien ◽  
G. N. Hinch ◽  
R. C. Dobos ◽  
G. Refshauge ◽  
...  

The present paper covers reproductive performance in an artificial-insemination (AI) program of the Sheep CRC Information Nucleus with 24 699 lambs born at eight locations in southern Australia across five lambings between 2007 and 2011. Results from AI with frozen semen compared well with industry standards for natural mating. Conception rates averaged 72%, and 1.45 lambs were born per ewe pregnant for Merino ewes and 1.67 for crossbreds. Lamb deaths averaged 21% for Merino ewes and 15% for crossbreds and 19%, 22% and 20% for lambs from ewes that were mated to terminal, Merino and maternal sire types, respectively. Net reproductive rates were 82% for Merino ewes and 102% for crossbreds. From 3198 necropsies across 4 years, dystocia and starvation-mismothering accounted for 72% of lamb deaths within 5 days of lambing. Major risk factors for lamb mortality were birth type (single, twin or higher order), birthweight and dam breed. Losses were higher for twin and triplet lambs than for singles and there was greater mortality at relatively lighter and heavier birthweights. We conclude that reproductive rate in this AI program compared favourably with natural mating. Lamb birthweight for optimum survival was in the 4–8-kg range. Crossbred ewes had greater reproductive efficiency than did Merinos.


1989 ◽  
Vol 49 (2) ◽  
pp. 291-297 ◽  
Author(s):  
D. Zygoyiannis ◽  
N. Katsaounis ◽  
G. Karatzas

ABSTRACTNinety-nine indigenous Greek goats (Capra prisca) on natural pasture were used to study reproductive performance as affected by method of breeding. At the beginning of the breeding season oestrus was induced with 45 mg fluorogestone acetate-impregnated vaginal sponges and 300 to 500 i.u. PMSG, given on day 15. The animals were separated into groups of 33 for mating: (i) by natural service (natural mating); (2) by artificial insemination with fresh semen (AI fresh); and (3) by insemination with frozen semen (AI frozen). Alpine bucks and Alpine semen were used for natural mating and AI of does.No effect of method of breeding was found on gestation length and sex ratio. Reproductive performance was significantly affected by method of breeding; at 21 days after insemination, fertility of does bred with fresh semen (69·7%) was comparable with that obtained with natural mating (72·7%) but the fertility of does bred with frozen semen (48·5%) was much lower. This difference was statistically significant at kidding (P < 0·05). Litter size was 2·14 and 1·86 (P < 0·05) for the AI fresh and AI frozen groups, respectively, and was 1·96 for the natural mating group. More multiple litters were obtained with the AI fresh group (P < 0·05). It is concluded that, induction and synchronization of oestrus and ovulation at the beginning of the breeding season gave reproductive performance with fresh semen comparable to that obtained with natural service, but use of frozen semen gave unsatisfactory results.


1979 ◽  
Vol 59 (4) ◽  
pp. 675-683 ◽  
Author(s):  
A. J. HACKETT ◽  
H. A. ROBERTSON ◽  
E. K. INSKEEP ◽  
J. N. B. SHRESTHA ◽  
M. S. WOLYNETZ

Synchronized estrus and ovulation were induced during the anestrous season (April–May 1974) in 373 ewes of three synthetic (one sire and two dam) strains and two unselected (Suffolk and Finnish Landrace) purebred strains by treatment with 30 mg fluorogestone acetate (FGA) impregnated in polyurethane intravaginal sponges for 12 days. Following sponge removal each ewe received 500 IU pregnant mares’ serum gonadotrophin (PMSG) IM. Of these, 167 were bred by artificial insemination (AI) at 48 and 60 h post sponge removal with 0.2 ml raw unextended semen collected by electroejaculation (EE). Five days after AI, ewes were exposed to a follow up ram for 16 days for subsequent mating if a second estrus occurred. The remaining 206 were exposed to rams for a period of 22 days for natural mating. Blood samples were collected from 69 ewes, 9, 19 and 27 days post sponge removal and analyzed for progesterone to ascertain if corpora lutea were formed and whether the ewes recycled. The age of ram by mating method interaction significantly affected both fertility and fecundity mainly because some of the younger rams lacked libido and experience for natural mating. There were no significant differences in prolificacy due to any of the main effects tested. Among the 69 ewes examined for progesterone levels, 93% had formed corpora lutea after hormone treatment and 16% recycled. Only 16 of the 255 ewes that did not conceive to the synchronized estrus lambed to the subsequent estrus.


2019 ◽  
Vol 59 (2) ◽  
pp. 225 ◽  
Author(s):  
Walvonvitis Baes Rodrigues ◽  
Jean do Prado Jara ◽  
Juliana Correa Borges ◽  
Luiz Orcirio Fialho de Oliveira ◽  
Urbano Pinto Gomes de Abreu ◽  
...  

The objective of this trial was to evaluate different post-timed artificial insemination (TAI) reproductive managements in postpartum beef cows to produce crossbred calves from artificial insemination (AI). Nellore cows (n = 607), with 45 days postpartum, were inseminated at a fixed time, using a protocol that included an intravaginal progesterone-releasing device along with oestradiol benzoate, prostaglandin, equine chorionic gonadotropin, and oestradiol cypionate, followed TAI 48 h post-device removal. Four post-TAI treatments were evaluated: in CONTROL (T1, n = 161), cows were exposed to Nellore clean-up bulls until the end of the breeding season (75 days). In OBSERVATION (T2, n = 132), heat detection was performed for 15–25 days post-TAI, followed by AI. In RESYNC22 (T3, n = 157) and RESYNC30 (T4, n = 157), resynchronisation started after 22 or 30 days, following second TAI at Day 32 or 40 days after first TAI. In T2, T3 and T4, after the second AI, cows were exposed to Nellore clean-up bulls until the end of the breeding season (75 days). The pregnancy rate (PR) for the first TAI did not differ (54.6%, 53.0%, 59.2%, and 51.6% for CONTROL, OBSERVATION, RESYNC 22, and RESYNC 30, respectively; P = 0.66), and no difference was observed for the second TAI (RESYNC 22 = 45.31% and RESYNC30 = 46.05%; P = 0.137), in the PR at the end of the breeding season (86.33%, 86.36%, 78.98%, and 81.52%, P = 0.43), or embryonic losses (4.54%, 2.85%, 6.45% and 7.40%, respectively; P = 0.61), but the percentage of crossbred pregnancy was higher in groups with resynchronisation (RESYNC22 and RESYNC30) than CONTROL and OBSERVATION (98.38%, 90.62%, 63.30%, 78.95%, P &lt; 0.001). In conclusion, resynchronisation programs of 22 or 30 days are more efficient to produce AI products, and the final pregnancy rate is similar among the treatments, differing only in the amount of calves produced by AI.


2022 ◽  
Author(s):  
Murat Can Demir ◽  
Cihan Kaçar ◽  
Umut Çağın Arı ◽  
Semra Kaya ◽  
Oğuz Merhan ◽  
...  

Abstract The present study aimed to determine the effects of progesterone-based gonadotropin-releasing hormone (GnRH), prostaglandin F2α (PGF2α), and equine chorionic gonadotropin (eCG) injections on progesterone profiles and pregnancy rates in cows with no estrus symptoms within 60 days after parturition. A total of 80 cows were included in the study. All animals had the progesterone-releasing device PRID®Delta placed intravaginally for nine days with an injection of GnRH. On the eighth day, PGF2α was injected, and PRID®Delta was removed from the vagina on day nine. Artificial insemination was carried out 60 hours after PRID®Delta removal. In half of the animals (n = 40), 600 IU of eCG was injected when PRID®Delta was removed on the ninth day before artificial insemination 60 hours later. Blood samples were taken from the tail vein on days 0 and 8 to determine progesterone levels. The pregnancy rate in the group that received eCG was 37.5%, while it was 27.5% in those that did not (P = 0.4). While the dominant follicle diameter was 15.5 mm in cows injected with eCG during timed artificial insemination, the follicle diameter was 12.4 mm in cows with no eCG injection (P <0.001). There were no differences in serum progesterone values in blood samples taken until the time of artificial insemination. However, progesterone values in the blood taken during artificial insemination were 0.94 ng/ml in the eCG- group and 0.72 ng/ml in the eCG+ group (P <0.05). As a result, it was determined that eCG injections, in addition to progesterone-based GnRH and PGF2α applications, increased the pregnancy rates in cows without symptoms of estrus. The dominant follicle diameter was larger in cows treated with eCG during artificial insemination; however, follicle size did not increase the pregnancy rate.


1991 ◽  
Vol 71 (1) ◽  
pp. 237-239 ◽  
Author(s):  
P. A. Thacker ◽  
A. D. Gooneratne ◽  
R. N. Kirkwood

Pregnancy rates and litter sizes following insemination of sows with fresh-extended and frozen-thawed semen averaged 71 vs. 53% and 10.6 vs. 4.4, respectively, and was not affected by the addition of 12.5 μg relaxin to semen. These results indicate no relaxin mediated effect on either pregnancy rate or litter size when sows are artificially inseminated with relaxin-supplemented semen. Key words: Artificial insemination, relaxin, sows


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