Effect of Rotation Number on Parameter of Fertilisation of Poligamously Mating Domestic Pigeons (Columba Livia L.)

In the rotation method to break the monogamous mating habits, 24 male and 24 female racing pigeons were used. They were didved into three groups, each consisting of 8 male and 8 feamle pigeons put separated in three huts A, B, C. At the first rotation (second mating) female pigeons of hut a were moved to hut B; all female pigeons of hut B to hut C while female pigeons of hut C took the place of female pigeons of hut A. The females were rotated sequentially three times and the male pigeons three times also. With regard to percentage fertilization and productivity rate, there were no significant differences at 5% level (P>0.05) among the rotations. The regression (b - 0.72%) of percentage fertilization on rotation number and the regression (b - 0.89%) of productivity rate on rotation number were found not to be significantly different from zero. The correlation coefficients attached to both regressions (r 0.41 and r -0.34) respectively were not significant at 5% level. The rotational mating method did not depress the parameters of fertility tested and is, as such a useful tool to induce pigeons to sequential polygamy.

HETEROSIS AND GRAIN YIELD AND COMPONENT IT BY USE (LINE×TESTER) OF INBRED LINES MAIZE

A field experiment was conducted at Northwest of Ramadi .Ten inbred lines by using Line×tester mating method. On spring season 2018 seeds were planted, at an thesis crossing between inbreds and tester was done. On fall season 2018 for crosses and parents using RCBD with three replications to evaluate and estimate heterosis. The results of statistical analysis for these parents and their crosses were highly significant differences for all traits. Genetic analysis shows superiority the inbred KR640 was best for plant height 183.16 cm, number of grain/row 34.34 grain and grain yield 148.43 g .The tester A105 gave high value in grain yield was 145.62 g . The cross (B73 × IK8) gave high leaves area 0.511 cm, no of grain per rows 39.21grain and grain yield 217.05 g. The crosses (B73 × IK8) gave highly positive hybrid vigor to better parent and mid parent for no. of grain per rows was %28.21 and %42.24 grain yield was %79.10 and %81.71. we can conclude the possibility of using superior inbreeds to produce hybrids or use selection for it and produce synthetic varieties.

Combining Ability of Sixteen USA Maize Inbred Lines and Their Outbreeding Prospects in China

In China, there is an increasing need for greater genetic diversity in maize (Zea mays L.) germplasm and hybrids appropriate for mechanical harvesting. In order to test and distinguish American maize inbred lines with exceptional combining ability, four Chinese maize inbred lines (Chang7-2, Zheng 58, four-144 and four-287) were used to judge the combining ability and heterosis of 16 USA inbred lines by a NCII genetic mating method. The results showed that among the American inbred lines, 6M502A, LH208, NL001, LH212Ht, PHW51, FBLA and LH181 expressed good GCA for yield characteristics; while RS710, PHP76, FBLA, and PHJ89 showed excellent GCA for machine harvesting characteristics. Five hybrids (NL001 × Chang7-2, LH212Ht × Chang7-2, FBLA × four-144, LH181 × four-287, PHK93 × four-287) had better SCA values for yield characteristics, at 1.69, 1.07, 1.48, 1.84 and 1.05, respectively; while NL001 × Chang 7-2, 6M502A × Chang7-2, LH212Ht × Chang7-2, LH181 × four-287, PHW51 × Chang7-2 had better TCA values for yield characteristics, at 3.03, 2.80, 2.41, 2.19 and 1.91, respectively; NL001 × Chang7-2, 6M502A × Chang7-2, LH212Ht × Chang7-2, LH181 × four-287, PHW51 × Chang7-2 showed excellent Control Heterosis values, with 21.48%, 19.64%, 15.93%, 14.05% and 11.60% increases, respectively, compared to the check and potential for future utilization in Inner Mongolian corn production.

Combining Ability of Sixteen USA Maize Inbred Lines and Their Outbreeding Prospects in China

In China, there is an increasing need for greater genetic diversity in maize (Zea mays L.) germplasm and hybrids appropriate for mechanical harvesting. In order to test and distinguish American maize inbred lines with exceptional combining ability, four Chinese maize inbred lines (Chang7-2, Zheng 58, four-144 and four-287) were used to judge the combining ability and heterosis of 16 USA inbred lines by a NCII genetic mating method. The results showed that among the American inbred lines, 6M502A, LH208, NL001, LH212Ht, PHW51, FBLA and LH181 expressed good GCA for yield characteristics; while RS710, PHP76, FBLA, and PHJ89 showed excellent GCA for machine harvesting characteristics. Five hybrids (NL001 × Chang7-2, LH212Ht × Chang7-2, FBLA × four-144, LH181 × four-287, PHK93 × four-287) had better SCA values for yield characteristics, at 1.69, 1.07, 1.48, 1.84 and 1.05, respectively; while NL001 × Chang 7-2, 6M502A × Chang7-2, LH212Ht × Chang7-2, LH181 × four-287, PHW51 × Chang7-2 had better TCA values for yield characteristics, at 3.03, 2.80, 2.41, 2.19 and 1.91, respectively; NL001 × Chang7-2, 6M502A × Chang7-2, LH212Ht × Chang7-2, LH181 × four-287, PHW51 × Chang7-2 showed excellent Control Heterosis values, with 21.48%, 19.64%, 15.93%, 14.05% and 11.60% increases, respectively, compared to the check and potential for future utilization in Inner Mongolian corn production.

Combining Ability of Sixteen U.S.A. Maize Inbred Lines and Utilization Potential as Hybrids in P.R.C

In China, there are increasing needs for greater genetic diversity of maize (Zea mays L.) germplasm and for hybrids appropriate for machine harvesting. to test and distinguish American maize inbred lines with exceptional combining ability, Four Chinese maize inbred lines (Chang7-2, Zheng 58,four-144 and four-287) were used to judge combining ability and heterosis of 16 U.S.A inbred lines by a NCII genetic mating method. The result showed: Among the American inbred lines, 6M502A,LH208,NL001,LH212Ht,PHW51,FBLA and LH181 showed excellent GCA for yield characteristics; while RS710,PHP76,FBLA,and PHJ89 showed excellent GCA for machine harvesting characteristics. In hybrid combinations, NL001×Chang7-2,LH212Ht×Chang7-2,FBLA×four-144,LH181×four-287,PHK93×four-287 had better SCA for yield characteristics; while NL001×Chang 7-2,6M502A×Chang7-2,LH212Ht×Chang7-2,LH181×four-287,PHW51×Chang7-2 were better than the check for machine harvesting characteristics. NL001×Chang7-2, 6M502A×Chang7-2,LH212Ht×Chang7-2,LH181×four-287,PHW51×Chang7-2 showed excellent total combined advantages compared to the check and potential for future utilization in Inner Mongolia corn production.

BINARY VECTOR CONSTRUCTION OF KAPPA(κ)-CARRAGEENASE GENE AND TRANSFORMATION TO Agrobacterium tumefaciens AS MEDIATOR FOR SEAWEED TRANSGENIC GENERATION

Increasing of kappa (κ)-carrageenan content in Kappaphycus alvarezii seaweed is potentially be achieved by applying transgenesis technology. This study was performed to obtain a construction of κ-Carrageenase gene and Agrobacterium tumefaciens to carry those construction genes. The κ-Carrageenase (κ-Car) gene was involved in κ-carrageenan biosynthesis. The κ-Car gene sequence was ligated between the 35S CaMV promoter and tNos terminator sequences to generate pMSH/κ-Car expression vector. Transformation of pMSH/κ-Car plasmid to Escherichia coli was performed by heat-shock method, and to Agrobacterium tumefaciens by tri-parental mating method. The results showed that several colonies of E. coli and A. tumefaciens grew in the selective culture mediums containing antibiotic. PCR analysis using primers 35S-Forward and tNos-Reverse with DNA template from those bacterial colonies resulted DNA fragment of about 2,000 bp, the same as the total length of 35S CaMV promoter, κ-Car gene and tNos terminator sequences. Therefore, the construction of pMSH/κ-Car gene was succeeded and a colony of A. tumefaciens transformant carrying pMSH/κ-Car plasmid was successfully produced. Keywords: Agrobacterium tumefaciens, kappa(κ)-Carrageenase gene, transgenesis, vector

Hubungan Berat Induk dan Berat Sapi Anak Sapi

ABSTRACT. A survey conducted to study a relationship between a dam body weight and a calf birth weight of cattle. There were six dams of Brahman Cross (B.C), six females calves of B.C dam, eleven local cattle dams and her eleven females calves, and another seven dams of local cattle and her seven male calves. The mean birth weight were local cattle and her seven male calves. The mean birth weight were 20.67 ±13.47 Kg for the female calf of B.C dam, and 19.27 ± 4.41 Kg for thr female calf of local cattle dam, and 18 ± 2.33 Kg for the male calf of local cattle dam. The mating method used was an artificial insemination and Brahman Cross frozen semen. The birth weight between the two groups of these female calves were not significantly different, and berween the two groubs of these female calves and the male calves were not significantly different. The body weights of B.C dams were not significantly correlated with their female calves ( r= + 0.7166, P0.05), and the local dam body weights were not significantly correlated with their female calves (r=+0.7450, P0.05), and with their male calves (r=+ 0.1948, P0.05). The equation of the regression lines were Y = 5.38 + 0.07 X for female calves and Y = 16.21 + 0.01 X for male calves. X dam body wight, and Y = calf birth weight.

KELAINAN RANGKA FETUS MENCIT (Mus musculus) GALUR BALB/C AKIBAT PEMBERIAN DEET (Diethyltoluamide)

<em>The study was aimed to identify the effect of DEET on fetal skeletal abnormalities of Balb/C strain mice. Thirty five female Balb/C strain mice were used in this study. The female mice were mated with male mice used one mating method. Mice in treatment group were administered dermally with 281,25; 562,5 1125 and 2250 mg/kg BW DEET disolved in ethanol at gestation day of 6 to 15, while mice in control were administered with ethanol. Mice in group were repeated seven times. At gestation day 18, the mice were sacrificed and fetal skeletal abnormalities were observed. Bone staining subsequently was done by using Alizarin Red S. Results were analyzed by using SPSS program version 16 personal computer, one way ANOVA and multiple comparison test with significance level of 0,05. Skeletal abnormalities such as supraoccipital, sternal, vertebral, sacrocaudal and phalanx were not found in all doses administered. From these experiment we suggested that DEET has no effects in fetal skeletal abnormalities such as supraoccipital, sternal, vertebral, sacrocaudal and phalanx</em>

Effect of mating method, sex and birth type on growth of lambs

Estrus synchronization methods was use to control the reproductive traits of sheep, as well as bringing more females at the same stage of estrus and ovulation. According to the points mentioned above, the aim of the present study was to investigate and compare mating method and influence of fixed factors on birth and weaning weight of lambs. Statistical analysis showed that exist difference in the body weights between genotypes of lambs. In the first group, the difference in weight of lambs at birth, regardless of mating method was not significant (P> 0.05), while in the second group, the difference was slightly significant (P <0.05). As for the birth type, sex and within the same genotype, there was a statistical significance (P <0.05) between singles obtained naturally, between the triplets obtained naturally and between triplets received hormonal method. All the differences between body weight at 30 days (mating method, sex and birth type under the same genotype) were statistically significant (P <0.05).The determined differences in the body weights at 60 days (sex, mating method and type of birth under the same genotype) were statistically significant (P <0.05). The results showed that the differences (mating method, sex and type of birth under the same genotype) were statistically significant (P <0.05), except in between body weight at 90 days in twins among two genotypes obtained by natural method, which were not statistically significant (P> 0.05).