scholarly journals Polymorphism detection of <i>DGAT1</i> and <i>Lep</i> genes in Anatolian water buffalo (<i>Bubalus bubalis</i>) populations in Turkey

2022 ◽  
Vol 65 (1) ◽  
pp. 1-9
Author(s):  
Raziye Işık ◽  
Emel Özkan Ünal ◽  
M. İhsan Soysal
Keyword(s):  
Milk Yield ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Diacylglycerol Acyltransferase ◽  
Synthesis Rate ◽  
Polymorphism Detection ◽  
Dgat1 Gene ◽  
Lep Gene ◽  
S Allele ◽  
Intron 2

Abstract. Acyl-CoA: diacylglycerol–acyltransferase 1 (DGAT1) enzyme plays a key role in controlling the synthesis rate triglyceride from diacylglycerol. Leptin (LP, OB, obese) is an important hormone that synthesizes mostly from adipose tissue and regulates glucose metabolism and homeostasis. DGAT1 and Lep genes are closely related to reproduction, growth, milk yield and composition in water buffalo breeds. This study aimed to identify genetic variation in the DGAT1 and Lep gene regions in 150 water buffalo individuals from five different provinces of Turkey using DNA sequencing. A total of 38 nucleotide variations and indels have identified 761 bp long partial intron 2 and exon 3 and 5′ UTR regions of the Lep gene in Anatolian water buffalo populations; 422 bp long partial exon 7–9 and exon 8 regions of DGAT1 gene were amplified and two mutations were defined in the point of 155 and 275 nucleotide that is three genotypes for S allele and Y allele of DGAT1 gene in intron 7 in Anatolian buffalo populations, respectively. These SNPs may have an effect on reproduction, growth, milk yield and composition in water buffalo populations and may prove to be useful for water buffalo breeding.

scholarly journals Traumatic Reticuloperitonitis in Water Buffalo (Bubalus bubalis): Clinical Findings and the Associated Inflammatory Response

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Maged El-Ashker ◽  
Mohamed Salama ◽  
Mohamed El-Boshy
Keyword(s):  
Inflammatory Response ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Clinical Findings ◽  
Necrosis Factor Alpha ◽  
Reactive Protein ◽  
Amyloid A ◽  
Diagnostic Usefulness ◽  
Factor Alpha ◽  
Traumatic Reticuloperitonitis

The present study was carried out to describe the clinical picture of traumatic reticuloperitonitis (TRP) in water buffalo (Bubalus bubalis) and to evaluate the inflammatory and immunologic responses for this clinical condition. Twenty-two buffalo with acute local TRP were monitored in our study. Additionally, 10 clinically healthy buffalo were randomly selected and served as controls. Acute local TRP was initially diagnosed by clinical examination and confirmed by ultrasonographic (USG) examination and/or necropsy findings. Blood samples were collected from all examined buffalo to measure the respective levels of tumor necrosis factor alpha (TNF-α), interleukin (IL)-1β, IL-6, IL-10 and interferon gamma (INF)-γ, serum amyloid A (SAA), C-reactive protein (CRP), haptoglobin (Hp), fibrinogen (Fb), and serum sialic acid (SSA). It was found that TNF-α, IL-1β, IL-6, IL-10, SAA, CRP, Hp, Fb, and SSA were significantly higher in buffalo with TRP than the controls. Our findings suggest that the examined immunologic variables were helpful in documenting the inflammatory response in buffalo with TRP. However, their diagnostic usefulness only becomes apparent when considered in tandem with the clinical findings for any given animal, its anamnesis, and a subsequent USG assessment. Due to the frequent complications of TRP, more accurate indicators of its occurrence and severity would be useful.

Comparison of feed intake, digestibility and fattening performance of Brahman grade cattle (Bos indicus) and crossbred water buffalo (Bubalus bubalis)

2004 ◽  
Vol 75 (6) ◽  
pp. 549-555 ◽  
Author(s):  
Rosalina M. LAPITAN ◽  
Arnel N. DEL BARRIO ◽  
Osamu KATSUBE ◽  
Tomomi TOKUDA ◽  
Edgar A. ORDEN ◽  
...  
Keyword(s):  
Feed Intake ◽  
Water Buffalo ◽  
Bos Indicus ◽  
Bubalus Bubalis ◽  
Fattening Performance

scholarly journals Reproductivity, Productivity and Management System of Indigenous Buffalo (Bubalus bubalis) Cows in Costal Areas of Pirojpur and Borguna District of Bangladesh

2014 ◽  
Vol 24 (1-2) ◽  
pp. 117-122 ◽  
Author(s):  
MR Karim ◽  
MZ Hossain ◽  
MR Islam ◽  
MS Parvin ◽  
MA Matin
Keyword(s):  
Birth Weight ◽  
Milk Yield ◽  
Management System ◽  
Post Partum ◽  
Bubalus Bubalis ◽  
Gestation Length ◽  
Calving Interval ◽  
Lactation Length ◽  
Daily Milk Yield ◽  
Daily Milk

Twenty five buffalo cows of Mothbaria Upazila of Pirojpur district and another twenty five indigenous buffalo cows of Pathorghata Upazila of Borguna district of Bangladesh were randomly selected and interviewed to collect data on reproductivity (gestation length, birth weight, age of first calving, post partum heat and calving interval), productivity (lactation length and daily milk yield) and management system (general management, housing, feeding, calf rearing and treatment facilities) of indigenous buffalo cows using pre-tested questionnaire during the period from January to June 2010. The reproductive parameters including gestation length, birth weight, age of first calving, post partum heat and calving interval of Mothbaria Upazila of Pirojpur district were 319.56 ± 5.93 days, 24.28 ± 4 kilograms, 50.88 ± 1.71 months, 153.6 ± 6.13 days, 547.92 ± 10.88 days respectively whereas in case of Pathorghata Upazila of Borguna district these parameters were 319.12 ± 4.69 days, 24.12 ± 3.6 kilograms, 51 ± 1.8 months, 153.44 ± 6.78 days, 547.24 ± 14.32 days respectively. The productive parameters including lactation length and daily milk yield of Mothbaria Upazila of Pirojpur district were 286.12 ± 11.27 days and 3.33 ± 0.68 liters/day respectively while in case of Pathorghata Upazila of Borguna district these parameters 290.44 ± 10.92 days and 3.43 ± 0.744 liters/day respectively. So, reproduction and production are almost regular comparing with other published reports with the exception of age of first calving which is almost 12 months later than the buffaloes of other countries. It could be concluded that the buffalo owners of these areas do not follow any scientific method of buffalo husbandry; they just follow traditional rearing system. The farmers mostly use buffaloes for draught purpose in these costal areas.DOI: http://dx.doi.org/10.3329/pa.v24i1-2.19113 Progress. Agric. 24(1&2): 117 - 122, 2013

scholarly journals Melanoma maligno em búfalos (Bubalus bubalis) albinos

2015 ◽  
Vol 36 (5) ◽  
pp. 3239 ◽  
Author(s):  
Maria Cecília Florisbal Damé ◽  
Clairton Marcolongo-Pereira ◽  
Letícia Fiss ◽  
Maria de Lourdes Adrién ◽  
Ana Lucia Schild
Keyword(s):  
Skeletal Muscle ◽  
Water Buffalo ◽  
Bubalus Bubalis ◽  
Renal Capsule ◽  
Southern Brazil ◽  
Epithelioid Cells ◽  
Melanocytic Tumors ◽  
Immunohistochemistry Staining ◽  
S 100 ◽  
Collagenous Stroma

<p>Two albino water buffalo affected by multiple melanocytic tumors in Southern Brazil are described. Grossly, there were multiple dark tumors within the skin, skeletal muscle, lungs, and lymph nodes. The tumor was also present in the pericardial sac, renal capsule, mediastinum and pleura. Microscopically, the tumors consisted of polyhedral epithelioid cells arranged in solid nests or interwoven fascicles supported by a thin and sparse collagenous stroma. The cytoplasm was eosinophilic and sometimes contained varying amounts of melanin pigment. The mitotic rates were low. Immunohistochemistry staining with Tyrosinase, Melan-A, Vimentin, S-100 protein, and neurofilament were positive. It is possible that the polymorphisms related to pigmentation in albino buffalo contributed with a high risk of developing melanoma as suggested in humans.</p>

scholarly journals Growth hormone (GH), prolactin (PRL), and diacylglycerol acyltransferase (DGAT1) gene polymorphisms in Turkish native cattle breeds

2015 ◽  
Vol 39 ◽  
pp. 734-748 ◽  
Author(s):  
Emel ÖZKAN ÜNAL ◽  
Eda Şeyma KEPENEK ◽  
Havva DİNÇ ◽  
Füsun ÖZER ◽  
Gizem SÖNMEZ ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Gene Polymorphisms ◽  
Diacylglycerol Acyltransferase ◽  
Cattle Breeds ◽  
Dgat1 Gene ◽  
Native Cattle
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