scholarly journals Wetland restoration and methanogenesis: the activity of microbial populations and competition for substrates at different temperatures

2009 ◽  
Vol 6 (6) ◽  
pp. 1127-1138 ◽  
Author(s):  
V. Jerman ◽  
M. Metje ◽  
I. Mandić-Mulec ◽  
P. Frenzel
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Iron Reduction ◽  
Carbon Mineralization ◽  
Marsh Soil ◽  
Ch4 Production ◽  
Different Temperatures ◽  
Acetoclastic Methanogenesis ◽  
Diverse Community ◽  
Water Saturated

Abstract. Ljubljana marsh in Slovenia is a 16 000 ha area of partly drained fen, intended to be flooded to restore its ecological functions. The resultant water-logging may create anoxic conditions, eventually stimulating production and emission of methane, the most important greenhouse gas next to carbon dioxide. We examined the upper layer (~30 cm) of Ljubljana marsh soil for microbial processes that would predominate in water-saturated conditions, focusing on the potential for iron reduction, carbon mineralization (CO2 and CH4 production), and methane emission. Methane emission from water-saturated microcosms was near minimum detectable levels even after extended periods of flooding (>5 months). Methane production in anoxic soil slurries started only after a lag period of 84 d at 15°C and a minimum of 7 d at 37°C, the optimum temperature for methanogenesis. This lag was inversely related to iron reduction, which suggested that iron reduction out-competed methanogenesis for electron donors, such as H2 and acetate. Methane production was observed only in samples incubated at 14–38°C. At the beginning of methanogenesis, acetoclastic methanogenesis dominated. In accordance with the preferred substrate, most (91%) mcrA (encoding the methyl coenzyme-M reductase, a key gene in methanogenesis) clone sequences could be affiliated to the acetoclastic genus Methanosarcina. No methanogens were detected in the original soil. However, a diverse community of iron-reducing Geobacteraceae was found. Our results suggest that methane emission can remain transient and low if water-table fluctuations allow re-oxidation of ferrous iron, sustaining iron reduction as the most important process in terminal carbon mineralization.

scholarly journals Wetland restoration and methanogenesis: the activity of microbial populations and competition for substrates at different temperatures

2009 ◽  
Vol 6 (1) ◽  
pp. 2357-2386 ◽  
Author(s):  
V. Jerman ◽  
M. Metje ◽  
I. Mandić-Mulec ◽  
P. Frenzel
Keyword(s):  
Methane Emission ◽  
Methane Production ◽  
Iron Reduction ◽  
Carbon Mineralization ◽  
Marsh Soil ◽  
Ch4 Production ◽  
Different Temperatures ◽  
Acetoclastic Methanogenesis ◽  
Diverse Community ◽  
Water Saturated

Abstract. Ljubljana marsh in Slovenia is a 16 000 ha area of partly drained fen, intended to be flooded to restore its ecological functions. The resultant water-logging may create anoxic conditions, eventually stimulating production and emission of methane, the most important greenhouse gas next to carbon dioxide. We examined the upper layer (~30 cm) of Ljubljana marsh soil for microbial processes that would predominate in water-saturated conditions, focusing on the potential for iron reduction, carbon mineralization (CO2 and CH4 production), and methane emission. Methane emission from water-saturated microcosms was near minimum detectable levels even after extended periods of flooding (>5 months). Methane production in anoxic soil slurries started only after a lag period and was inversely related to iron reduction, which suggested that iron reduction out-competed methanogenesis for electron donors, such as H2 and acetate. Methane production was observed only in samples incubated at 14–38°C. At the beginning of methanogenesis, acetoclastic methanogenesis dominated. In accordance with the preferred substrate, most (91%) mcrA (encoding the methyl coenzyme-M reductase, a key gene in methanogenesis) clone sequences could be affiliated to the acetoclastic genus Methanosarcina. No methanogens were detected in the original soil. However, a diverse community of iron-reducing Geobacteraceae was found. Our results suggest that methane emission can remain transient and low if water-table fluctuations allow re-oxidation of ferrous iron, sustaining iron reduction as the most important process in terminal carbon mineralization.

scholarly journals Modeling anaerobic soil organic carbon decomposition in Arctic polygon tundra: insights into soil geochemical influences on carbon mineralization

2019 ◽  
Vol 16 (3) ◽  
pp. 663-680 ◽  
Author(s):  
Jianqiu Zheng ◽  
Peter E. Thornton ◽  
Scott L. Painter ◽  
Baohua Gu ◽  
Stan D. Wullschleger ◽  
...  
Keyword(s):  
Temperature Sensitivity ◽  
Iron Reduction ◽  
Carbon Mineralization ◽  
Co2 Production ◽  
Model Framework ◽  
Binding Model ◽  
Microbial Decomposition ◽  
Carbon Decomposition ◽  
Ch4 Production ◽  
Wide Range

Abstract. Rapid warming of Arctic ecosystems exposes soil organic matter (SOM) to accelerated microbial decomposition, potentially leading to increased emissions of carbon dioxide (CO2) and methane (CH4) that have a positive feedback on global warming. Current estimates of the magnitude and form of carbon emissions from Earth system models include significant uncertainties, partially due to the oversimplified representation of geochemical constraints on microbial decomposition. Here, we coupled modeling principles developed in different disciplines, including a thermodynamically based microbial growth model for methanogenesis and iron reduction, a pool-based model to represent upstream carbon transformations, and a humic ion-binding model for dynamic pH simulation to build a more versatile carbon decomposition model framework that can be applied to soils under varying redox conditions. This new model framework was parameterized and validated using synthesized anaerobic incubation data from permafrost-affected soils along a gradient of fine-scale thermal and hydrological variabilities across Arctic polygonal tundra. The model accurately simulated anaerobic CO2 production and its temperature sensitivity using data on labile carbon pools and fermentation rates as model constraints. CH4 production is strongly influenced by water content, pH, methanogen biomass, and presence of competing electron acceptors, resulting in high variability in its temperature sensitivity. This work provides new insights into the interactions of SOM pools, temperature increase, soil geochemical feedbacks, and resulting CO2 and CH4 production. The proposed anaerobic carbon decomposition framework presented here builds a mechanistic link between soil geochemistry and carbon mineralization, making it applicable over a wide range of soils under different environmental settings.

scholarly journals The Effect of Rumination Time on Milk Performance and Methane Emission of Dairy Cows Fed Partial Mixed Ration Based on Maize Silage

Animals ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 50
Author(s):  
Robert Mikuła ◽  
Marcin Pszczola ◽  
Katarzyna Rzewuska ◽  
Sebastian Mucha ◽  
Włodzimierz Nowak ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Yield ◽  
Methane Emission ◽  
Methane Production ◽  
Production Traits ◽  
Maize Silage ◽  
Ch4 Production ◽  
Holstein Friesian ◽  
Milk Cows ◽  
Milk Performance

The objective of this study was to determine the effect of the rumination time on milk yield and composition as well as methane emission during lactation in high-yielding dairy cows fed a partial mixed ration based on maize silage without pasture access. A total of 365 high-yielding Polish Holstein-Friesian multiparous dairy cows were included in the study covering 24 to 304 days of lactation. Methane emission, rumination time, and milk production traits were observed for the period of 12 months. Next, the data from the cows were assigned to three groups based on daily rumination time: low rumination up to 412 min/day (up to 25th rumination percentile), medium rumination from 412 to 527 min/day (between the 25th and 75th percentile), and high rumination above 527 min/day (from the 75th percentile). Rumination time had no effect on milk yield, energy-corrected milk yield, or fat and protein-corrected milk yield. High rumination time had an effect on lower fat concentration in milk compared with the medium and low rumination groups. The highest daily CH4 production was noted in low rumination cows, which emitted 1.8% more CH4 than medium rumination cows and 4.2% more than high rumination cows. Rumination time affected daily methane production per kg of milk. Cows from the high rumination group produced 2.9% less CH4 per milk unit compared to medium rumination cows and 4.6% in comparison to low rumination cows. Similar observations were noted for daily CH4 production per ECM unit. In conclusion, a longer rumination time is connected with lower methane emission as well as lower methane production per milk unit in high-yielding dairy cows fed a maize silage-based partial mixed ration without pasture access.

scholarly journals Modeling anaerobic soil organic carbon decomposition in Arctic polygon tundra: insights into soil geochemical influences on carbon mineralization

2018 ◽  
Author(s):  
Jianqiu Zheng ◽  
Peter E. Thornton ◽  
Scott L. Painter ◽  
Baohua Gu ◽  
Stan D. Wullschleger ◽  
...  
Keyword(s):  
Temperature Sensitivity ◽  
Iron Reduction ◽  
Carbon Mineralization ◽  
Co2 Production ◽  
Model Framework ◽  
Binding Model ◽  
Microbial Decomposition ◽  
Carbon Decomposition ◽  
Ch4 Production ◽  
Wide Range

Abstract. Rapid warming of Arctic ecosystems exposes soil organic matter (SOM) to accelerated microbial decomposition, potentially leading to increased emissions of carbon dioxide (CO2) and methane (CH4) that have a positive feedback on global warming. The fate of permafrost carbon is determined in large part by soil moisture, and a significant portion of carbon may thaw in wet, anoxic conditions. Current estimates of the magnitude and form of carbon emissions from Earth system models include significant uncertainties since the models do not explicitly represent anaerobic carbon decomposition. Here we coupled modeling principles developed in different disciplines, including a thermodynamically based microbial growth model for methanogenesis and iron reduction, a pool-based model to represent upstream carbon transformations, and a humic ion-binding model for dynamic pH simulation to build a more versatile carbon decomposition model framework that can be applied to soils under varying redox conditions. This new model framework was parameterized and validated using synthesized anaerobic incubation data from permafrost affected soils along a gradient of fine-scale thermal and hydrological variabilities across Arctic polygonal tundra. The model accurately simulated anaerobic CO2 production and its temperature sensitivity using data on labile carbon pools and fermentations rates as model constraints. CH4 production is strongly influenced by water content, pH, methanogen biomass, and presence of competing electron acceptors, resulting in high variability in its temperature sensitivity. This work provides new insights into the interactions of SOM pools, temperature increase, soil geochemical feedbacks, and resulting CO2 and CH4 production. The proposed anaerobic carbon decomposition framework presented here builds a mechanistic link between soil geochemistry and carbon mineralization, making it applicable over a wide range of soils under different environmental settings.

Enhanced iron reduction by iron supplement: A strategy to reduce methane emission from paddies

2005 ◽  
Vol 37 (11) ◽  
pp. 2150-2154 ◽  
Author(s):  
Udo Jäckel ◽  
Salvatore Russo ◽  
Sylvia Schnell
Keyword(s):  
Methane Emission ◽  
Iron Reduction ◽  
Iron Supplement

scholarly journals Reaction Kinetics of Iron Oxides in Ok Tedi Magnetite Skarn Ore

2020 ◽  
pp. 1-13
Author(s):  
Mary Kama ◽  
Kaul Gena ◽  
Tindi Seje Nuru
Keyword(s):  
Reaction Kinetics ◽  
Papua New Guinea ◽  
Iron Reduction ◽  
New Guinea ◽  
Reaction Rate Constant ◽  
Close Monitoring ◽  
Significant Difference ◽  
Different Temperatures ◽  
C 30 ◽  
Kinetics Of

Magnetic skarn ore (MSO) is one of the major copper bearing ore extracted by the Ok Tedi Copper Mine in Papua New Guinea (PNG). Copper minerals are recovered by flotation while the iron not associated with copper are discarded as tailings. The objective of this investigation was to determine the iron ore reduction kinetics for the Ok Tedi MSO and ascertain if it can be processed to produce sponge iron for a mini steel plant in Papua New Guinea. SEM-EDAX analyses of the Ok Tedi MSO indicated 10.1% C, 30% O, 0.6% Mg, 1.1% Si, 21.1% S, 0.8% Ca and 36.2 % Fe. Most of the iron is in sulfide form. Both naturally occurring and roasted sinters of Ok Tedi MSO samples of different particle sizes were reduced by charcoal carbon at three different temperatures and seven different reduction times. Analyses of the reduced products indicated a metallic iron content of more than 65 wt. % on average. Results showed that there was no significant difference in reduction between fluxed and control materials. Only a slight increase in kinetics with reduced particle size, hence the reaction rate constant (K) did not vary much within the temperatures investigated. Reaction kinetics increases with increasing reduction time at 900°C. Therefore, more iron reduction is observed with particles of 106 µm size. In addition, the results also confirmed that the reduction energy used was higher at 800°C and lower at 1000°C. In conclusion, iron reduction can be improved but close monitoring of temperature and reduction times are essential to determine the reaction kinetics of the Ok Tedi MSO.

scholarly journals Pomegranate seed oil rich in conjugated linolenic acids reduces in vitro methane production

1970 ◽  
Vol 46 (3) ◽  
pp. 325-335
Author(s):  
E. Maleki ◽  
G.Y. Meng ◽  
M. Faseleh Jahromi ◽  
R. Jorfi ◽  
A. Khoddami ◽  
...  
Keyword(s):  
Methane Production ◽  
Volatile Fatty Acids ◽  
Seed Oil ◽  
Control Diet ◽  
Nutrient Utilization ◽  
Gas Production ◽  
Metabolizable Energy ◽  
Ruminal Fermentation ◽  
Ch4 Production

The objective of this study was to determine the effect of pomegranate (Punica granatum L.) seed oil (PSO) on gas and methane (CH4) production, ruminal fermentation and microbial populations under in vitro conditions. Three treatments consisting of a control diet containing 10 mg tallow (CON); the control diet with 5 mg PSO + 5 mg tallow (MPSO) and the control diet containing 10 mg PSO (HPSO) were compared. Ten mg of the experimental fat/oil samples were inserted into a gas-tight 100 mL plastic syringe containing 30 mL of an incubation inoculum and 250 mg of a basic substrate of a hay/concentrate (1/1, w/w) mixture. In vitro gas production was recorded over 0, 2, 4, 6, 8, 10, 12 and 24 h of incubation. After 24 hours, incubation was stopped, and methane production, pH, volatile fatty acids (VFAs) and microbial counts were measured in the inoculant. Gas production at 4, 6, 8, 10, 12 and 24 h incubation, metabolizable energy and in vitro organic matter disappearance increased linearly and quadratically as level of PSO increased. Furthermore, the 10 mg PSO (HPSO) decreased CH4 production by 21.0% compared with the control (CON) group. There were no significant differences in total and individual VFA concentrations between different levels of PSO, except for butyric acid. After 24 h of incubation, methanogenesis decreased in the HPSO compared with the MPSO and CON treatments. In addition, total bacteria and protozoa counts increased with rising PSO levels, while population methanogenesis declined significantly. These results suggested that PSO could reduce methane emissions, which might be beneficial to nutrient utilization and growth in ruminants.

The effects of essential oil and condensed tannin on fermentation and methane production under in vitro conditions

2016 ◽  
Vol 56 (10) ◽  
pp. 1707 ◽  
Author(s):  
Brittany Pinski ◽  
Mevlüt Günal ◽  
Amer A. AbuGhazaleh
Keyword(s):  
Fatty Acid ◽  
Methane Production ◽  
Volatile Fatty Acid ◽  
Condensed Tannin ◽  
Total Volatile ◽  
Tannin Extract ◽  
Ch4 Production ◽  
N Concentration ◽  
Total Volatile Fatty Acid

The potential of five different essential oils (EO) and quebracho condensed tannin extract (QCT) as antimethanogenic additives in ruminant feeds were investigated. The first experiment was conducted to screen the effects rosemary oil, sage oil, cinnamon oil (CNO), eucalyptus oil and myrrh oil at 500 mg/L of culture fluid on methane (CH4) production under in vitro conditions. Rumen contents were collected from a cannulated Holstein dairy cow and used for a 24-h batch-culture experiment. Treatments were a control (CON) or CON plus EO at 500 mg/L. Results showed that CNO decreased CH4 production and, therefore, was selected for Experiment 2. The second experiment was designed to test the effects of CNO at three different dose levels on CH4 production and fermentation in 24-h batch-culture experiments. Treatments were CON or CON plus CNO supplemented at 125, 250 and 500 mg/L. Relative to CON, CNO decreased total gas production at the 250 and 500 mg/L doses. All doses of CNO decreased CH4 production. Total volatile fatty acid production was lower in cultures incubated with CNO at the 500 mg/L. Ammonia-N concentration decreased in cultures incubated with CNO at the 500 mg/L. The third experiment was designed to test the effects of QCT on CH4 production and fermentation in 24-h batch cultures. Treatments were CON or CON plus QCT at 25, 50 and 75 g/kg of diet DM. Relative to CON, total volatile fatty acid concentration increased with the 50 g/kg QCT, but was similar to the 25 and 75 g/kg QCT. The proportions of acetate decreased, while the proportions of propionate increased with the 25 g/kg QCT compared with CON. Methane production was not affected in cultures incubated with QCT. Relative to CON, all doses of QCT decreased ammonia-N concentration. In conclusion, results from the present study showed that except for CNO, EO tested in the study had no effects on rumen CH4 production. Addition of CNO to rumen cultures at 125 and 250 mg/L reduced CH4 production without negative effects on rumen fermentation. Quebracho condensed tannin-extract supplementation had no effects on CH4 production and fermentation parameters except for ammonia-N concentration.

scholarly journals Multi-year effect of wetting on CH<sub>4</sub> flux at taiga–tundra boundary in northeastern Siberia deduced from stable isotope ratios of CH<sub>4</sub>

2019 ◽  
Vol 16 (3) ◽  
pp. 755-768 ◽  
Author(s):  
Ryo Shingubara ◽  
Atsuko Sugimoto ◽  
Jun Murase ◽  
Go Iwahana ◽  
Shunsuke Tei ◽  
...  
Keyword(s):  
Water Saturation ◽  
Methane Flux ◽  
Interannual Variations ◽  
Ch4 Emission ◽  
Ch4 Flux ◽  
Ch4 Production ◽  
Year Effect ◽  
Northeastern Siberia ◽  
Order Of Magnitude ◽  
Acetoclastic Methanogenesis

Abstract. The response of CH4 emission from natural wetlands due to meteorological conditions is important because of its strong greenhouse effect. To understand the relationship between CH4 flux and wetting, we observed interannual variations in chamber CH4 flux, as well as the concentration, δ13C, and δD of dissolved CH4 during the summer from 2009 to 2013 at the taiga–tundra boundary in the vicinity of Chokurdakh (70∘37′ N, 147∘55′ E), located on the lowlands of the Indigirka River in northeastern Siberia. We also conducted soil incubation experiments to interpret δ13C and δD of dissolved CH4 and to investigate variations in CH4 production and oxidation processes. Methane flux showed large interannual variations in wet areas of sphagnum mosses and sedges (36–140 mg CH4 m−2 day−1 emitted). Increased CH4 emission was recorded in the summer of 2011 when a wetting event with extreme precipitation occurred. Although water level decreased from 2011 to 2013, CH4 emission remained relatively high in 2012, and increased further in 2013. Thaw depth became deeper from 2011 to 2013, which may partly explain the increase in CH4 emission. Moreover, dissolved CH4 concentration rose sharply by 1 order of magnitude from 2011 to 2012, and increased further from 2012 to 2013. Large variations in δ13C and δD of dissolved CH4 were observed in 2011, and smaller variations were seen in 2012 and 2013, suggesting both enhancement of CH4 production and less significance of CH4 oxidation relative to the larger pool of dissolved CH4. These multi-year effects of wetting on CH4 dynamics may have been caused by continued soil reduction across multiple years following the wetting. Delayed activation of acetoclastic methanogenesis following soil reduction could also have contributed to the enhancement of CH4 production. These processes suggest that duration of water saturation in the active layer can be important for predicting CH4 emission following a wetting event in the permafrost ecosystem.

scholarly journals Identification of naturally available forage species and their feeding effect on dairy cows in different climatic vulnerable areas of Bangladesh

2017 ◽  
Vol 46 (2) ◽  
pp. 150-158
Author(s):  
SMR Rahman ◽  
NR Sarker ◽  
MR Amin ◽  
M Kamruzzaman ◽  
MR Haque
Keyword(s):  
Milk Production ◽  
Methane Emission ◽  
Methane Production ◽  
Check List ◽  
Climatic Zones ◽  
Forage Species ◽  
Different Types ◽  
Prone Area ◽  
Flood Prone Area ◽  
Drought Prone Area

An investigation was carried out with the objectives to identify the naturally occurring forage species, seasonal availability, production patterns under different climatic zones and production performance and methane emission from dairy cow under existing feeding systems. For this purpose, three different agro-climatic zones of Bangladesh, namely saline prone area (Satkhira), flood plain/river basin areas (Pabna), semi-arid/drought prone areas (Chapainobabgonj) were selected. To achieve the objectives, three Focus Group Discussions (FGD) were conducted in each location to obtain more information from different age groups of farmers. A total of 9 FDGs were conducted under three selected locations and twelve participants were attended in each FGD. During FDGs, information was collected through participatory discussions through check list and also discussion was recorded to verify the information gathered as per check list.  After collection of information in each side, all the data were checked and analyzed. The results indicated that in saline area, farmers reported that different types of local grass e.g. Tale Shapna,Durba,Nona Shapna, Khud Gate/ KhudKhachra, Shama, Full Paira, Bass Pata, Math Pora/KhataShak, GhimeeShak and Baksha etc were available round the year but according to their observation Nona Shapna, Tale Shapna and Baksha were more available compared to other species of the natural grasses and these three natural forages are more suitable in this area. In the drought prone area, different types of native grasses e.g. Durba,Shama, Mutha,Katla,Kausha/Kannar, Binna, Datuloka,Shanchi, Shunshue, Bash Batari, Ulo and Binna Pati were identified and utilized by the farmers in different seasons but Durba,Katla and Mutha were found more drought tolerant compared to other species. In flood prone area, Kolmi, Shanti, Baksha, Arail, Dubla, Bokma, Vadail and Bolenga etc were found and Kolmi, Baksha and Arail are more suitable in this area. Farmers were also reported that fodder tree like Dumur/khoksha also is survive in water logging situation and or flood prone area. The study revealed that calculated total DMI (Kg/h/day) was the highest (14.14±1.06) in flood prone  followed by drought (13.80±1.30) and saline areas (4.43±0.20),  respectively. Similarly, the milk production was also higher (12.06±1.19 litre/h/day) in flood prone area followed by drought (4.47±0.60 litre/h/day) and saline (1.83±0.11 litre/h/day) areas, respectively. The calculated total methane emission (g/h/d) was significantly higher in flood prone (478.31±36.36) and the lowest in saline (153.35±7.14) prone areas. Whereas, methane production per unit of milk yield, was the lowest in flood prone (46.55±6.78) and the highest (110.48±21.69) in drought prone area and the difference was statistically significant (p<0.05).  Therefore, it may be concluded that farmers’ rearing animals under climate vulnerable areas utilizing natural grasses are more prone to higher methane production compared to animals rearing better feed resources though their availability was varied with the seasons and locations.  Hence, further research is needed to explore more suitable natural grasses in addition to introduction of high yielding fodder with higher biomass and nutritive values based on the existing cropping systems in those climate vulnerable areas for higher milk production and low enteric methane emission in the country.Bang. J. Anim. Sci. 2017. 46 (2): 150-158

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