scholarly journals Characterization on the rhizoremediation of petroleum contaminated soil as affected by different influencing factors

2010 ◽  
Vol 7 (3) ◽  
pp. 4665-4688 ◽  
Author(s):  
J. Tang ◽  
R. Wang ◽  
X. Niu ◽  
M. Wang ◽  
Q. Zhou
Keyword(s):  
Plant Growth ◽  
Influencing Factors ◽  
Tall Fescue ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Initial Period ◽  
Plant Growth Promoting Rhizobacteria ◽  
Slow Increase ◽  
Continuous Increase ◽  
Petroleum Contaminated Soil

Abstract. In this paper, pilot experiments were conducted to analyze the effect of different environmental factors on the rhizoremediation of petroleum contaminated soil. Different plant species (cotton, ryegrass, tall fescue, and alfalfa), addition of fertilizer, different concentration of TPH in soil, bioaugmentation with effective microbial agent (EMA) and PGPR, and remediation time were tested as influencing factors during bioremediation process of Total Petroleum Hydrocarbon (TPH). The result shows that the remediation process can be enhanced by different plants species with the following order: tall fescue > ryegrass > alfalfa > cotton. The degradation rate of TPH increased with increased fertilizer addition and moderate level of 20 g/m2 urea is best for both plant growth and TPH remediation. High TPH content is toxic to plant growth and inhibits the degradation of petroleum hydrocarbon with 5% TPH content showing the best degradation result in soil planted with ryegrass. Bioaugmentation with different bacteria and plant growth promoting rhizobacteria (PGPR) showed the following results for TPH degradation: cotton + EMA + PGPR > cotton + EMA > cotton + PGPR > cotton > control. Rapid degradation of TPH was found at the initial period of remediation caused by the activity of microorganisms, continuous increase was found from 30–90 d period and slow increase was found from 90 to 150 d. The result suggests that rhizoremediation can be enhanced with the proper control of different influencing factors that affect both plant growth and microbial activity in the rhizosphere environment.

scholarly journals Characterisation of the rhizoremediation of petroleum-contaminated soil: effect of different influencing factors

2010 ◽  
Vol 7 (12) ◽  
pp. 3961-3969 ◽  
Author(s):  
J. C. Tang ◽  
R. G. Wang ◽  
X. W. Niu ◽  
M. Wang ◽  
H. R. Chu ◽  
...  
Keyword(s):  
Plant Growth ◽  
Influencing Factors ◽  
Plant Species ◽  
Tall Fescue ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Degradation Rate ◽  
Initial Period ◽  
Continuous Increase ◽  
Petroleum Contaminated Soil

Abstract. Pilot experiments were conducted to analyse the effect of different environmental factors on the rhizoremediation of petroleum-contaminated soil. Different plant species (cotton, ryegrass, tall fescue and alfalfa), the addition of fertilizer, different concentrations of total petroleum hydrocarbons (TPH) in the soil, bioaugmentation with effective microbial agents (EMA) and plant growth-promoting rhizobacteria (PGPR) and remediation time were tested as influencing factors during the bioremediation process of TPH. The results show that the remediation process can be enhanced by different plant species. The order of effectiveness of the plants was the following: tall fescue > ryegrass > alfalfa > cotton. The degradation rate of TPH increased with increased fertilizer addition, and a moderate urea level of 20 g N (Nitrogen)/m2 was best for both plant growth and TPH remediation. A high TPH content is toxic to plant growth and inhibits the degradation of petroleum hydrocarbons. The results showed that a 5% TPH content gave the best degradation in soil planted with ryegrass. Bioaugmentation with different bacteria and PGPR yielded the following results for TPH degradation: cotton+EMA+PGPR > cotton+EMA > cotton+PGPR > cotton > control. Rapid degradation of TPH was found at the initial period of remediation caused by the activity of microorganisms. A continuous increase of degradation rate was found during the 30–90 days period followed by a slow increase during the 90–150 days period. These results suggest that rhizoremediation can be enhanced with the proper control of different influencing factors that affect both plant growth and microbial activity in the rhizosphere environment.

Isolation of ACC Deaminase-Containing Plant Growth-Promoting Rhizobacteria from Petroleum Contaminated Soil

2011 ◽  
Vol 356-360 ◽  
pp. 244-247
Author(s):  
Chang Hong Guo ◽  
Fang Fang ◽  
Jia Li Liu
Keyword(s):  
Economic Development ◽  
Plant Growth ◽  
Contaminated Soil ◽  
Acc Deaminase ◽  
Nitrogen Sources ◽  
Plant Growth Promoting Rhizobacteria ◽  
16S Rdna Sequence ◽  
Plant Growth Promoting ◽  
Petroleum Contaminated Soil ◽  
Growth Promoting

Petroleum is the most important energy for economic development in China and other countries. But in the process of mining, storage and producing, it has caused many aspects of pollution. In this study, a bacterial strain A-3 which could use ACC as the sole nitrogen sources was isolated from petroleum-contaminated soil. The strain was identified as Achromobacter, according to the results of morphology, physiology and the phylogenetical analyses of 16S rDNA sequence. Strain Achromobacter A-3 could produce ACC deaminase, and could be used for remediation of petroleum-contaminated areas in future.

Experimental Study on Bioremediation of Petroleum Contaminated Soil in Puyang Oilfield

2011 ◽  
Vol 233-235 ◽  
pp. 693-696
Author(s):  
Li Chen ◽  
Fa Wang Zhang ◽  
Shuo Ren ◽  
Sheng Zhang
Keyword(s):  
Experimental Study ◽  
Nitrogen Fixation ◽  
Contaminated Soil ◽  
Petroleum Hydrocarbon ◽  
Degradation Rate ◽  
Soil Layer ◽  
Water Oxygen ◽  
Petroleum Contaminated Soil ◽  
Ecological Remediation ◽  
Distinct Increase

According to the previous experience on remediation experiment in laboratory and field, the study on microgial ecological remediation of petroleum contaminated soil in Puyang oilfield was carried out under 3 different conditions. Here the results from remediation show that the technology of microbe cooperated with alfalfa, single microbe technology and single alfalfa technology all possess remedial effect on petroleum contaminated soil through 99-day period remediation, and the technology of microbe cooperated with alfalfa creates the best remedial mission with petroleum hydrocarbon degradation rate of 65.27%, while the rest single technologies exerts degradation rate of about 40% on petroleum hydrocarbon, Moreover, the experiment results indicate that few nutriment such as soluble salt, NO3-, Cl-,etc can infiltrate into the lower soil layer(50cm). However, the distinct increase of NH4+ in the second and third remediation area may attribute to abundance fertilizer transportation into depth soil layer due to the nitrogen fixation of alfalfa’s roots. In addition, we also find that the remediation effect can be impacted by the factors containing tempertation, water, oxygen, nutriment and mini-geo-enviroment.

scholarly journals Effects of surfactants on the remediation of petroleum contaminated soil and surface hydrophobicity of petroleum hydrocarbon degrading flora

2020 ◽  
Vol 26 (5) ◽  
pp. 200384-0
Author(s):  
Jianbo Liu ◽  
Liming Xu ◽  
Feifei Zhu ◽  
Shouhao Jia
Keyword(s):  
Contaminated Soil ◽  
Petroleum Hydrocarbons ◽  
Petroleum Hydrocarbon ◽  
Initial Conditions ◽  
Removal Rate ◽  
Surface Hydrophobicity ◽  
Orthogonal Experiments ◽  
Petroleum Contaminated Soil ◽  
Application Potential ◽  
High Removal Rate

It has been proven that surfactants used in the remediation of petroleum hydrocarbon contaminated soil have great application potential. In this study, the effects of five surfactants (SDBS, Tween80, Tween60, rhamnolipid and TRS-1) on leaching of petroleum hydrocarbons from soil were investigated through orthogonal experiments, and petroleum hydrocarbon components were analyzed by GC/MS. The effects of surfactants on the degradation of petroleum hydrocarbon were analyzed by the changes of microbial growth curve and surface hydrophobicity. The results showed that surfactant type, temperature and surfactant concentration had significant effects on the removal rate of petroleum hydrocarbon. Tween80, rhamnolipid and TRS-1 have good bio-friendliness and a high removal rate of petroleum hydrocarbons (up to 65%), suitable for the restoration of the soil used in the experiment And Surfactants exhibited a higher removal rate for small molecules and petroleum hydrocarbons with odd carbon atoms. Surfactants have a certain modification effect on the surface of relatively hydrophilic bacteria under the initial conditions, making their surface properties develop in the direction of enhanced hydrophobicity, and the hydrophobicity has increased from less than 20% to about 40%.

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