scholarly journals TRYPANOSOMA spp. in captive primates in a brazilian zoo

2021 ◽  
Vol 50 (2) ◽  
pp. 121-134
Author(s):  
Wesley Jose dos Santos ◽  
Livia Maisa Guiraldi ◽  
Mirian Dos Santos Paixão Marques ◽  
Maria Fernanda Alves-Martin ◽  
Gabriela Pacheco Sanchez ◽  
...  
Keyword(s):  
Genetic Material ◽  
Antibody Test ◽  
Conventional Polymerase Chain Reaction ◽  
Bed Nets ◽  
Trypanosoma Rangeli ◽  
Trypanosoma Lewisi ◽  
Polymerase Chain ◽  
Mechanical Devices ◽  
Zoological Park ◽  
Different Sources

Captive animals, despite the constant care provided, are susceptible to infections from different sources. We herein report the natural trypanosome infection of 11 (28.2% positive) out of 39 non-human primates from 13 different species, in a Brazilian zoological park. Immunofluorescent antibody test (IFAT) and conventional polymerase chain reaction (cPCR) ruled out Trypanosoma cruzi, the etiological agent of Chagas disease. However, sequencing performed with positive samples employing hsp70 primers revealed similarities from 86% to 88% to diverse trypanosomes, including T. cruzi, Trypanosoma grayi, Trypanosoma lewisi, Trypanosoma rangeli and Trypanosoma vivax. We believe that the low similarity values obtained by sequencing reflect the difficulties in the molecular identification of trypanosomes, which share a large portion of their genetic material; this similarity may also preclude the diagnosis of co-infection by more than one trypanosome species. Thus, our study demonstrates the presence of diverse trypanosomes in primates, which are susceptible to infection by these parasites. Mechanical devices such as windows and bed nets, etc., are required to avoid vector insects in these environments, in addition to preventive quarantining of animals recently introduced into zoos. Therefore, investigation of the parasites in both the animals already residing in the zoo and those being introduced is of paramount importance, although no easy task. KEY WORDS: Non-human primates; monkey; diagnosis; trypanosomes.

scholarly journals A Comparative Analysis of Polymerase Chain Reaction and Direct Fluorescent Antibody Test for Diagnosis of Genital Herpes

2017 ◽  
Vol 9 (01) ◽  
pp. 053-056 ◽  
Author(s):  
Vrushali Patwardhan ◽  
Preena Bhalla ◽  
Deepti Rawat ◽  
Vijay Kumar Garg ◽  
Kabir Sardana ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Genital Herpes ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Conventional Polymerase Chain Reaction ◽  
Genital Ulcer ◽  
Chain Reaction ◽  
Direct Fluorescent Antibody ◽  
Polymerase Chain ◽  
Simplex Virus

ABSTRACT Objective: To compare laboratory tests that can simultaneously detect and type herpes simplex virus (HSV) directly from the genital ulcer specimens in clinically suspected cases of genital herpes. Materials and Methods: A study was conducted over 10 months and 44 adult male and female patients clinically suspected with genital herpes were recruited. Genital ulcer swab specimens were subjected to glycoprotein-G gene-based conventional polymerase chain reaction (PCR) and commercially available direct fluorescent antibody (DFA) test and the results were compared. Results: PCR for HSV was positive in 82% (36/44) cases. DFA was positive in 68.2% (30/44) cases. There was 100% agreement between HSV types detected by DFA and PCR. The strength of agreement between the results was better in primary genital herpes than recurrent cases. Conclusion: PCR was found to be better in the detection of HSV in recurrent genital herpes patients. It is a better modality, especially when genital herpes clinically presents with ulcerative or crusted lesions, and is also a cheaper alternative as compared to DFA.

scholarly journals Rickettsia typhi IN RODENTS AND R. felis IN FLEAS IN YUCATÁN AS A POSSIBLE CAUSAL AGENT OF UNDEFINED FEBRILE CASES

2015 ◽  
Vol 57 (2) ◽  
pp. 129-132 ◽  
Author(s):  
Gaspar PENICHE-LARA ◽  
Karla DZUL-ROSADO ◽  
Carlos PÉREZ-OSORIO ◽  
Jorge ZAVALA-CASTRO
Keyword(s):  
Rattus Rattus ◽  
Conventional Polymerase Chain Reaction ◽  
Causal Agent ◽  
Wild Rodents ◽  
Murine Typhus ◽  
Rickettsia Typhi ◽  
Rickettsia Felis ◽  
Vector Borne ◽  
Polymerase Chain ◽  
First Time

Rickettsia typhi is the causal agent of murine typhus; a worldwide zoonotic and vector-borne infectious disease, commonly associated with the presence of domestic and wild rodents. Human cases of murine typhus in the state of Yucatán are frequent. However, there is no evidence of the presence of Rickettsia typhi in mammals or vectors in Yucatán. The presence of Rickettsia in rodents and their ectoparasites was evaluated in a small municipality of Yucatán using the conventional polymerase chain reaction technique and sequencing. The study only identified the presence of Rickettsia typhi in blood samples obtained from Rattus rattus and it reported, for the first time, the presence of R. felis in the flea Polygenis odiosus collected from Ototylomys phyllotis rodent. Additionally, Rickettsia felis was detected in the ectoparasite Ctenocephalides felis fleas parasitizing the wild rodent Peromyscus yucatanicus. This study’s results contributed to a better knowledge of Rickettsia epidemiology in Yucatán.

scholarly journals Detection of MYCN Amplification in Serum DNA Using Conventional Polymerase Chain Reaction

2016 ◽  
Vol 31 (9) ◽  
pp. 1392 ◽  
Author(s):  
Youngeun Ma ◽  
Ji Won Lee ◽  
Soo Jin Park ◽  
Eun Sang Yi ◽  
Young Bae Choi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Conventional Polymerase Chain Reaction ◽  
Mycn Amplification ◽  
Chain Reaction ◽  
Serum Dna ◽  
Polymerase Chain

scholarly journals Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil

2020 ◽  
Vol 41 (6) ◽  
pp. 2687-2694
Author(s):  
Gabriela Döwich Pradella ◽  
◽  
Taiane Acunha Escobar ◽  
Claudia Acosta Duarte ◽  
Irina Lübeck ◽  
...  
Keyword(s):  
Rural Areas ◽  
Direct Sequencing ◽  
Geographical Area ◽  
Genetic Material ◽  
Rio Grande ◽  
Sand Fly ◽  
Rio Grande Do Sul ◽  
Transmission Area ◽  
Leishmania Spp ◽  
Polymerase Chain

Visceral leishmaniasis (VL) is a zoonosis caused by the protozoan of the genus Leishmania. The disease is transmitted by the bite of a sand fly vector. Although the main reservoirs are dogs, other hosts can be infected and may play this role. Rio Grande do Sul western region, located on the triple border of Brazil-Uruguay-Argentina, represents a VL transmission area. The goal of the present study was to identify Leishmania spp. infection in animals from rural areas of Uruguaiana, Rio Grande do Sul. Nine farms in the Uruguaiana municipality, Rio Grande do Sul state, were included. Peripheral blood samples were collected from 113 animals (canine [n=22], equine [n=91]) for detection of Leishmania spp. DNA was isolated and polymerase chain reaction was performed. Eight (7%) animals with Leishmania spp. infection were detected on two farms in the same geographical area, seven of which were horses and one was canine, all of which were asymptomatic. To investigate the species of Leishmania, one of the positive equine samples was subjected to direct sequencing, which confirmed the presence of L. infantum genetic material. Results of this study confirm the presence of L. infantum-infected animals in rural areas of Uruguaiana, and provide evidence supporting further investigation of risk factors for dissemination in such areas.

Molecular detection of visceral leishmaniasis in dogs from Barão de Melgaço, Pantanal region of Mato Grosso, Brazil

2021 ◽  
Vol 41 ◽  
Author(s):  
Álvaro Felipe L.R. Dias ◽  
Arleana B.P.F. Almeida ◽  
Luciano Nakazato ◽  
Valéria R.F. Sousa
Keyword(s):  
Visceral Leishmaniasis ◽  
Molecular Detection ◽  
Clinical Signs ◽  
Conventional Polymerase Chain Reaction ◽  
Epidemiological Survey ◽  
Mato Grosso ◽  
Canine Infection ◽  
Polymerase Chain ◽  
Comparison Of The Results ◽  
Low Prevalence

ABSTRACT: The increasing expansion of visceral leishmaniasis (VL) in the Brazilian territory evidences the need for studies focused on the main reservoir of this parasite: the dog. This study aimed to conduct an epidemiological survey in the municipality of Barão de Melgaço, Pantanal region of the state of Mato Grosso (MT), Brazil. Conventional polymerase chain reaction (PCR) and qualitative SYBR®Green real-time PCR (qPCR) were used to diagnose canine VL (CVL) and characterize the factors associated with this infection. Of the 402 dogs that had blood samples collected, 31 presented the parasite DNA, representing a prevalence of 7.71% in the population studied. Positivity indices for PCR and qPCR were 3.48 (14/402) and 7.21% (29/402), respectively. Comparison of the results obtained by both techniques showed moderate agreement (Kappa = 0.5364). Of the independent variables analyzed, presence of clinical signs (p≤0.05) was the only one associated with CVL. Based on this study, we conclude that VL is a circulating disease, with relatively low prevalence, in dogs of Barão de Melgaço/MT, and that the presence of clinical signs is the only variable associated with canine infection.

scholarly journals Monitoring of rotavirus in treated wastewater in Tehran with a monthly interval, in 2017–2018

2020 ◽  
Vol 18 (6) ◽  
pp. 1065-1072
Author(s):  
Shadi Tavakoli Nick ◽  
Seyed Reza Mohebbi ◽  
Seyed Masoud Hosseini ◽  
Hamed Mirjalali ◽  
Masoud Alebouyeh
Keyword(s):  
Rna Extraction ◽  
Conventional Polymerase Chain Reaction ◽  
Treated Wastewater ◽  
Harsh Environments ◽  
Monthly Interval ◽  
Rt Pcr ◽  
Conventional Pcr ◽  
Wastewater Treatment Process ◽  
Polymerase Chain ◽  
Elution Method

Abstract Rotaviruses are among the major causes of viral acute gastroenteritis in newborns and children younger than 5 years worldwide. The ability of rotaviruses to remain infectious in harsh environments as well as in the wastewater treatment process makes them one of the most prevalent enteric viruses. The current study aimed to determine the presence of rotavirus genomes and to analyze them phylogenetically in secondary treated wastewater (TW) samples. In total, 13 TW samples were collected from September 2017 to August 2018. Viral concentration was carried out using the absorption-elution method, and after RNA extraction and cDNA synthesis, real-time and conventional polymerase chain reaction (PCR) were performed. A phylogenetic tree was drawn using Maximum Likelihood and Tamura 3-parameter using MEGA v.6 software. Rotavirus genomes were detected in 7/13 (53.8%) and 3/13 (23.07%) samples using reverse transcription (RT)-PCR and conventional PCR, respectively. Accordingly, phylogenetic analysis revealed G4P[8], G9P[4], and G9P[8] genotypes among the samples. The presence of rotavirus in secondary TW samples discharged into surface water emphasizes the importance of monitoring and assessing viral contamination in the water sources used for agricultural and recreational purposes.

scholarly journals Prevalence and genetic characterization of Toxoplasma gondii in naturally infected backyard pigs intended for familial consumption in Romania

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Anamaria Ioana Paştiu ◽  
Anamaria Cozma-Petruț ◽  
Aurélien Mercier ◽  
Anamaria Balea ◽  
Lokman Galal ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Rural Areas ◽  
Antibody Test ◽  
Antibody Detection ◽  
Serum Samples ◽  
Potential Threat ◽  
Polymerase Chain ◽  
Pcr Targeting ◽  
Backyard Pigs

Abstract Background Foodborne toxoplasmosis in humans can be due to the exposure to tissue cysts of Toxoplasma gondii through the consumption of meat, including pork, of infected animals. Traditional Romanian food habits include pork as the preferred meat, while backyard pig rearing remains a common practice in many rural areas of Romania. The aims of the present study were to estimate the prevalence of T. gondii infection in naturally infected backyard pigs slaughtered for familial consumption and to genetically characterize the T. gondii strains obtained. Methods Paired blood and heart samples were collected from 94 backyard pigs, home slaughtered for private consumption. Serum samples were analyzed using the immunofluorescence antibody test (IFAT) for anti-T. gondii antibody detection. Heart samples were screened by polymerase chain reaction (PCR) targeting the 529-bp repeat region (REP529) for T. gondii detection. In addition, heart samples from IFAT positive animals were bioassayed in mice. The T. gondii isolates were genotyped by the analysis of 15 microsatellite markers. Results The results showed that almost half of the pigs investigated were T. gondii seropositive (46.8%, 95% confidence interval (CI): 36.4–57.4%) and in more than a quarter of the pigs (26.6%, 95% CI: 18.0–36.7%), the parasite was detected by PCR. Three (3/44) T. gondii strains were isolated from hearts of seropositive pigs and they all belonged to genotype II. Conclusions The present study showed the presence of T. gondii infection in backyard pigs in Romania, which suggests that consumption of pork from animals reared and slaughtered at home may pose a potential threat to human health and should be given attention. In addition, to our knowledge, this is the first study to provide data concerning T. gondii strains circulating in pigs from Romania.

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