scholarly journals Identification of Leishmania spp. in horses and a dog from rural areas of Uruguaiana, Rio Grande do Sul, Brazil

2020 ◽  
Vol 41 (6) ◽  
pp. 2687-2694
Author(s):  
Gabriela Döwich Pradella ◽  
◽  
Taiane Acunha Escobar ◽  
Claudia Acosta Duarte ◽  
Irina Lübeck ◽  
...  
Keyword(s):  
Rural Areas ◽  
Direct Sequencing ◽  
Geographical Area ◽  
Genetic Material ◽  
Rio Grande ◽  
Sand Fly ◽  
Rio Grande Do Sul ◽  
Transmission Area ◽  
Leishmania Spp ◽  
Polymerase Chain

Visceral leishmaniasis (VL) is a zoonosis caused by the protozoan of the genus Leishmania. The disease is transmitted by the bite of a sand fly vector. Although the main reservoirs are dogs, other hosts can be infected and may play this role. Rio Grande do Sul western region, located on the triple border of Brazil-Uruguay-Argentina, represents a VL transmission area. The goal of the present study was to identify Leishmania spp. infection in animals from rural areas of Uruguaiana, Rio Grande do Sul. Nine farms in the Uruguaiana municipality, Rio Grande do Sul state, were included. Peripheral blood samples were collected from 113 animals (canine [n=22], equine [n=91]) for detection of Leishmania spp. DNA was isolated and polymerase chain reaction was performed. Eight (7%) animals with Leishmania spp. infection were detected on two farms in the same geographical area, seven of which were horses and one was canine, all of which were asymptomatic. To investigate the species of Leishmania, one of the positive equine samples was subjected to direct sequencing, which confirmed the presence of L. infantum genetic material. Results of this study confirm the presence of L. infantum-infected animals in rural areas of Uruguaiana, and provide evidence supporting further investigation of risk factors for dissemination in such areas.

Potential Vectors of Leishmania Parasites in a Recent Focus of Visceral Leishmaniasis in Neighborhoods of Porto Alegre, State of Rio Grande do Sul, Brazil

2020 ◽  
Vol 57 (4) ◽  
pp. 1286-1292 ◽  
Author(s):  
Felipe Dutra Rêgo ◽  
Getúlio Dornelles Souza ◽  
Júlia Bahia Miranda ◽  
Lais Vieira Peixoto ◽  
José Dilermando Andrade-Filho
Keyword(s):  
Visceral Leishmaniasis ◽  
Rio Grande ◽  
Abundant Species ◽  
Sand Fly ◽  
Leishmania Braziliensis ◽  
Sand Flies ◽  
Rio Grande Do Sul ◽  
Porto Alegre ◽  
Polymerase Chain

Abstract In view of recent cases of human and canine visceral leishmaniasis reported in Porto Alegre, Rio Grande do Sul, Brazil, we investigated the sand fly fauna inhabiting the neighborhoods of Morro Santana and Jardim Carvalho, Brazil, continuing a series of entomological surveys aimed to identify potential vectors of Leishmania (Ross, 1903) parasites. Sand flies were collected monthly from October 2016 to October 2017 using CDC light traps in the intradomiciliary and peridomiciliary environments of seven residences. Sand fly abundance was correlated to climatic variables. Females were pooled by species, location, and date for Leishmania DNA molecular screening using ITS1 and kDNA polymerase chain reaction. In total, 501 sand flies from five species were collected in which Lutzomyia gaminarai (Cordero, Vogelsang & Cossio, 1928) (Diptera: Psychodidae) (78%) was the most abundant species in the intradomiciliary sites while Migonemyia migonei (Franca, 1920) (Diptera: Psychodidae) (43.3%) was the most abundant in the peridomiciliary sites. A higher number of sand flies were collected during the warmest months, from December to March (Mann–Whitney statistical test – P < 0.001). Leishmania infantum DNA was detected in Lu. gaminarai (2), Pintomyia fischeri (Pinto, 1926) (1) and Mg. migonei (1). Leishmania braziliensis DNA was detected in Lu. gaminarai (1) and Pi. fischeri (1). Our results add support to the possible vector role of Pi. fischeri in the epidemiological cycle of Le. infantum in Brazil. Furthermore, the first documented detection of Leishmania DNA in Lu. gaminarai may be indicative of multiple vectors being involved in the Leishmania cycle within Porto Alegre.

CONTRIBUIÇÃO AO CONHECIMENTO DA AVIFAUNA DO MUNICÍPIO DE TAQUARA, RIO GRANDE DO SUL

2019 ◽  
Vol 2 ◽  
pp. 28
Author(s):  
Marcelo Pereira de Barros ◽  
Ana Paula Lima da Silveira ◽  
Bruna Reis Ferreira
Keyword(s):  
Rural Areas ◽  
Rio Grande ◽  
Bird Community ◽  
Frequency Of Occurrence ◽  
Diverse Group ◽  
Rio Grande Do Sul ◽  
Natural Ecosystems ◽  
Environmental Disturbances ◽  
Environmental Pressures ◽  
Hydrographic Basin

As aves destacam-se dentro dos vertebrados por serem um grupo diverso e adaptado a diferentes ambientes, a composição da comunidade de aves de um determinado local é um importante bioindicador ambiental, pois ela reflete alterações recentes ou pretéritas de determinada área. Na Bacia Hidrográfica do Rio dos Sinos, as pressões sobre os remanescentes de ecossistemas naturais são intensas em decorrência da urbanização crescente, do estabelecimento de indústrias e da ampliação das fronteiras agrícolas, esses fatores associados geram a redução e fragmentação de habitats, com impactos irrecuperáveis para a flora e a fauna. Devido à escassez de dados sobre a avifauna do município de Taquara, o presente estudo teve como objetivo levantar informações sobre o grupo, listando as espécies de aves locais. Entre agosto de 2016 e junho de 2017 foram realizadas quatro amostragens mensais em duas áreas rurais do município, totalizando 44 campanhas de observação e 132 horas de esforço amostral. As espécies foram identificadas e classificadas conforme sua frequência de ocorrência mensal, em espécies comuns e raras. Foram registradas 133 espécies distribuídas em 47 famílias, o que representa mais de 20% das espécies ocorrentes para o estado. Ao longo do período amostrado, 90 espécies foram classificadas como comuns e 47 consideradas raras para as áreas pesquisadas.Palavras-chave: Aves. Bacia hidrográfica do Rio dos Sinos. Preservação.ABSTRACTBirds stand out within vertebrates because they are a diverse group adapted to different environments. The composition of the bird community of a certain place is an important environmental bioindicator since it reflects recent or previous environmental disturbances of a certain area. In the Sinos River Basin, environmental pressures on the remnants of natural ecosystems are intense as a result of increasing urbanization, the establishment of industries and expansion of agricultural frontiers which generate habitat reduction and fragmentation with irrecoverable damages on flora and fauna. Due to the scarcity of data on the avifauna of the municipality of Taquara, the present study aimed to gather information and list the species of local birds. Between August 2016 and June 2017, four monthly samplings were carried out in two rural areas of the municipality, totaling 44 observation campaigns and 132 hours of sample effort. The species were identified and classified as common and rare according to their frequency of occurrence. There were 133 species distributed in 47 families, representing more than 20% of the species that occurred in the state. During the sampled period, 90 species were classified as common and 47 were considered rare for the areas surveyed.Keywords: Birds. Hydrographic basin of Sinos River. Preservation.

scholarly journals The Analysis of Water Availability as an Important Factor for Farmers' Permanence in the Field: A Study in Southern Brazilian Countryside

2019 ◽  
Vol 7 (10) ◽  
pp. 296-306
Author(s):  
José Luis Perlin ◽  
Dieison Prestes da Silveira ◽  
Jana Koefender ◽  
Roberta Cattaneo Horn ◽  
Juliane Nicolodi Camera ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Water Availability ◽  
Rural Areas ◽  
Rio Grande ◽  
Southern Brazil ◽  
Rio Grande Do Sul ◽  
Qualitative And Quantitative ◽  
Fundamental Factor ◽  
Family Farmers

Water availability in rural areas is essential to production activities and quality of life. The aim of the current study is to evaluate the key role played by water resources in rural properties as a factor for farmers' permanence in the field in Southern Brazil. The research followed a qualitative and quantitative approach, which encompassed the application of semi-structured questionnaires to family farmers living in Marmeleiro and Fontana Freda communities, in the rural area of Jaguari County, Rio Grande do Sul State, Brazil. Based on collected data, participants have shown knowledge about aspects such as water quality and availability, as well as about the preservation of this resource. Nevertheless, it was clear that water availability is a fundamental factor for farmers to remain in the field.

Rio Grande do sul in the Portuguese Empire: The Formative Years, 1777-1808

1983 ◽  
Vol 39 (4) ◽  
pp. 519-535 ◽  
Author(s):  
Rudy Bauss
Keyword(s):  
Economic Growth ◽  
Rio De Janeiro ◽  
Geographical Area ◽  
Rio Grande ◽  
Colonial Period ◽  
Rio Grande Do Sul ◽  
Crucial Importance ◽  
Economic Activities ◽  
Portuguese Empire ◽  
Formative Years

This analysis and survey of economic activities in Rio Grande do Sul aim to shed new light on the crucial importance of the southernmost captaincy to overall Brazilian development at the close of the colonial period. Rio Grande do Sul was unique in economic growth, compared to the Center-South, Northeast and North, because the extreme South produced for a variety of markets: the internal Brazilian, the internal Portuguese, the Portuguese reexport to Europe and the Rio de Janeiro reexport to Angola. This market diversification made Rio Grande do Sul less dependent on the commercial vagaries of any particular geographical area. The focus of this article is the importance of economic growth in Rio Grande do Sul prior to 1808.

TGFA/TAQ I Polymorphism in Nonsyndromic Cleft Lip and Palate Patients from Rio Grande Do Sul, Brazil

2008 ◽  
Vol 45 (5) ◽  
pp. 539-544 ◽  
Author(s):  
Ângela Ehlers Bertoja ◽  
Clarice Sampaio Alho ◽  
Everaldo De França ◽  
Beatriz Menegotto ◽  
Wanyce Miriam Robinson
Keyword(s):  
Cleft Lip ◽  
Cleft Lip And Palate ◽  
Control Sample ◽  
Rio Grande ◽  
Control Group ◽  
Buccal Swab ◽  
Rio Grande Do Sul ◽  
Polymerase Chain ◽  
Genic Mutation ◽  
Control Study

Objective: To test the TGFA/Taq I polymorphism in the development of nonsyndromic cleft lip and palate. Design And Setting: The research was based on a case-control study, including nonsyndromic cleft lip and palate patients (140 individuals) and a control sample of unaffected individuals (142) to ascertain the absence or presence of genic mutation at the TGFA locus. Interventions: The DNA of carriers of nonsyndromic cleft lip with or without cleft palate was obtained by buccal swab, and the DNA of the control group was extracted from peripheral blood leucocytes. TGFA/Taq I polymorphism was determined genetically by polymerase chain reaction using specific primers and fragment digestion with Taq I restriction enzyme. Results: No significant association was detected when patients and controls were compared with the genotype for TGFA/Taq I polymorphism. Conclusion: Mutations in TGFA gene have no association with nonsyndromic cleft lip and palate in the sample from Rio Grande do Sul. Therefore, based on this study, it is not possible to determine the role played by TGFA in the expression of cleft lip and palate.

scholarly journals Serological and molecular investigation of Leishmania spp. infection in cats from an area endemic for canine and human leishmaniasis in Northeast Brazil

2019 ◽  
Vol 28 (4) ◽  
pp. 790-796 ◽  
Author(s):  
José Artur Brilhante Bezerra ◽  
Ilanna Vanessa Pristo de Medeiros Oliveira ◽  
Ana Carolina Yamakawa ◽  
Mariana Guimarães Nilsson ◽  
Klívio Loreno Raulino Tomaz ◽  
...  
Keyword(s):  
Risk Factors ◽  
Leukemia Virus ◽  
Genetic Material ◽  
Clinical Signs ◽  
Rio Grande ◽  
Feline Leukemia Virus ◽  
Pcr Analysis ◽  
Blood Samples ◽  
Human Leishmaniasis ◽  
Leishmania Spp

Abstract The aim of this study was to investigate the occurrence of Leishmania spp. antibodies, and its association with feline immunodeficiency virus (FIV) and feline leukemia virus (FeLV), in domestic cats from an area endemic for canine and human leishmaniasis in Rio Grande do Norte State, Brazil. Ninety-one cats were subjected to a complete clinical exam, and blood samples were collected. An epidemiological questionnaire was used to investigate the risk factors. IgG anti-Leishmania spp. antibodies were detected by immunofluorescence antibody test (IFAT), with a cut-off value of 1:40. Polymerase chain reaction (PCR) was performed to detect genetic material of Leishmania spp. in the blood samples. The presence of antibodies against FIV and antigens of FeLV was evaluated using an immunochromatographic test. Seropositivity for Leishmania spp., FIV, and FeLV was observed in 14/91 (15.38%), 26/91 (28.57%), and 3/91 (3.29%) cats, respectively. All samples gave negative results on PCR analysis. Based on these data, no significant statistical association was observed between seropositivity for Leishmania spp., and sex, age, presence of clinical signs, evaluated risk factors, and positivity for retroviruses. These findings demonstrated for the first time that cats from Mossoró, Rio Grande do Norte, are being exposed to this zoonosis and might be part of the epidemiological chain of transmission of visceral leishmaniasis.

scholarly journals Epidemiological characterization of Leishmaniasis in the rural area of the western border region of Rio Grande do Sul, Brazil

2021 ◽  
Author(s):  
Gabriela Döwich Pradella ◽  
Claudia Acosta Duarte ◽  
Taiane Acunha Escobar ◽  
Luísa Zuravski ◽  
Geórgia Camargo Góss ◽  
...  
Keyword(s):  
Risk Factors ◽  
Logistic Regression ◽  
Rural Areas ◽  
Protective Factor ◽  
Descriptive Analysis ◽  
Clinical Signs ◽  
Rio Grande ◽  
Border Region ◽  
Rio Grande Do Sul ◽  
Western Border

Abstract Background: Leishmaniasis, zoonotic diseases, are in the top ten list of tropical neglected disease globally. The number of contaminated dogs in South America is estimated in millions of animals and correlated to the human’s cases, especially in Brazil. Equines became infected too and can play a role in the epidemiological chain. Thus, the aim of the present study was to evaluate risk factors to leishmaniasis in rural areas of the western border region of Rio Grande do Sul state, Brazil by Leishmania sp. protozoa molecular detection and serological evaluation (ELISA) in equine and canine blood samples. Methods: This work included nine farms around the city of Uruguaiana. It was collected epidemiologic information regarding farm characteristics and biologic material collection of canine and equine, totalizing 113 samples. The polymerase chain reaction (PCR) technique was used to detect Leishmania spp. in biological samples. Variables related to the farm were collected and evaluated through descriptive analysis followed by chi-square test and a logistic regression analysis.Results: Nineteen positive samples (19/113 – 16,81%) were detected, being 18 equines and 1 canine, in six of the nine farms included in the study. No animal showed clinical signs of the disease. According to the variables analyzed, when compared each characteristic separately, the presence of abundant vegetation and poor hygiene demonstrated to be risk factors to Leishmania infection in rural areas. The logistic regression showed that excellent general hygiene, proximity to the weir and trimmed grass were protective factors (p=0.038, p=0.001 and p=0.014, respectively). Having excellent hygiene represents a 70% lower chance of getting contaminated, keeping the grass cut protects the animal by more than 90% and the proximity of the weir represents a protective factor of 96%.Conclusions: The presence of leishmaniasis in the western border region of Rio Grande do Sul was 16,81% and it was influence by farm characteristics. The role of the excellent general hygiene as a protective factor is extremally relevant in the leishmaniosis prevention.

scholarly journals ‘Encantos de São Chico’: A Touristic Routing Proposal

2021 ◽  
Vol 13 (2) ◽  
pp. 601-619
Author(s):  
Vanessa Karine Spindler ◽  
◽  
Celmar Correa de Oliveira ◽  
Angela Cristina Albarello Marins ◽  
Clodis de Oliveira Andrades Filho ◽  
...  
Keyword(s):  
Sustainable Development ◽  
Environmental Sustainability ◽  
Rural Areas ◽  
Local Development ◽  
Rio Grande ◽  
Rural Tourism ◽  
Rio Grande Do Sul ◽  
Tourism Activity ◽  
Sustainable Regional Development ◽  
Two Stages

Tourism in São Francisco de Paula, in Rio Grande do Sul [Brazil] has stood out and, due to its wide territory, the need to organize tourism in rural areas emerges. Otherwise, socioeconomic and environmental sustainability may be weakened, if the presence of tourism activity is disordered. Thus, tourist routing can be an ally in promoting local, sustainable development. Therefore, the objective of this study was to develop a roadmap for rural tourism in São Francisco de Paula. The first step towards achieving this goal involved examining documents to obtain an overview of local tourism. The next two stages took place through a specific questionnaire addressed to entrepreneurs and tourists. Based on the data collected, an instrument for sustainable regional development was developed: a rural roadmap called ‘Encantos de São Chico’. The survey shows that both owners and tourists recognize the relevance of rural tourism to sustainable local development.

scholarly journals VINHO E CULTURA ITALIANA: ASPECTOS PRESENTES NA PAISAGEM RURAL DE NOVA PALMA, RS / BRASIL WINE AND ITALIAN CULTURE: ASPECTS OF GIFTS IN RURAL LANDSCAPE OF NOVA PALMA, RS, BRAZIL

2016 ◽  
Vol 2 (2) ◽  
pp. 234
Author(s):  
Vanessa Manfio
Keyword(s):  
Collective Memory ◽  
Rural Areas ◽  
Identity Element ◽  
Rio Grande ◽  
Rural Landscape ◽  
Research Approach ◽  
Rio Grande Do Sul ◽  
The People ◽  
Italian Culture ◽  
Local Residents

O vinho é um elemento importante da cultura italiana, pois permite a associação da identidade e memória coletiva, resultando em paisagens repletas de nostalgia e materialidade. No Brasil, especialmente no Rio Grande do Sul, a colonização italiana contribuiu para o aparecimento de várias paisagens vitícolas, já que o vinho é um elemento identitário deste povo. Dessa forma, este artigo apresenta uma reflexão sobre a cultura italiana e a paisagem vitícola presentes no espaço rural de Nova Palma, objetivando analisar o vinho como elemento da paisagem e da cultura italiana do povo deste espaço, através da abordagem da pesquisa qualitativa com realização de entrevistas semi-estruturas e revisão de literatura. Pretendendo assim, contribuir para as discussões sobre a cultura italiana, paisagem e o vinho. Os elementos ligados ao universo do vinho estão presentes na paisagem rural de Nova Palma - RS, cuja tradição e sentimento de pertencimento a cultura italiana são fortemente visíveis nos moradores do local através das formas espaciais, valores e hábitos. Neste município os vinhedos centenários, herança da colonização italiana, dividem espaços com novos cultivos de videira e com a fabricação artesanal do vinho.Abstract:Wine is an important part of Italian culture, because it allows the combination of identity and collective memory, resulting in landscapes full of nostalgia and materiality. In Brazil, especially in Rio Grande do Sul, the Italian colonization contributed to the emergence of several wine-growing landscapes, since wine is an identity element of this people. Thus, this article presents a reflection on Italian culture and wine landscape present in the rural areas of Nova Palma, aiming to analyze the wine as landscape element and the Italian culture of the people of this area, through the qualitative research approach to conducting semi-structures interviews and literature review. Intending thus contribute to the discussions about the Italian culture, landscape and wine. The elements connected to the wine universe are present in the countryside of Nova Palma- RS, whose tradition and feeling of belonging to Italian culture are highly visible in the local residents through the spatial forms, values and habits. In this municipality the Centennial Vineyards, heritage of Italian colonization, share space with new vine crops and the artisanal manufacture of wine.Keywords: Italian colonization; Rural areas; Identity.

Sign in / Sign up

Export Citation Format

Share Document