scholarly journals Dependence of the state of the large intestine parietal microflora on the activity of Helicobacter pylori associated chronic gastritis

2021 ◽  
Vol 20 (3) ◽  
pp. 44-48
Author(s):  
H. B. Isayev ◽  
M. R. Ibrahimov ◽  
T. H. Salimov
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Histological Examination ◽  
Mucous Membrane ◽  
Large Intestine ◽  
Chronic Gastritis ◽  
Rapid Urease Test ◽  
E Coli ◽  
Urease Test ◽  
H Pylori

Introduction. The aim of the study was to detect the dependence of the composition of the parietal microflora of the colon on the activity of Helicobacter pylori-associated chronic gastritis and the degree of Helicobacter pylori contamination of the gastric mucosa. Materials and methods. The study was carried out on 50 patients aged 24 to 72 years, who were diagnosed with HP-associated chronic gastritis in the phase of exacerbation based on the results of FEGDS and histological examination of biopsies of the mucous membrane of the antrum of the stomach in combination with a rapid urease test. A series of dilutions 10¹-10⁹ was prepared from a homogenized biopsy specimen of the mucous membrane of the sigmoid colon in 0.1 ml of isotonic sodium chloride solution, and 0.1 ml of a solution of the corresponding dilution was inoculated onto the surface of the nutrient medium. The study of mucosal microflora was carried out by means of bacteriological examination of biopsies of the mucous membrane of the sigmoid and cecum. The degree of H. pylori contamination of the gastric mucosa was assessed in the course of histological examination by a semi-quantitative method: low degree of contamination — up to 20 microbial bodies, average — 20-50 microbial bodies, strong — over 50 microbial bodies in the field of view. Results. The study demonstrated that the indicators of the average concentrations of bifidobacteria, lactobacilli and typical strains of E. coli progressively decreased with the transition to a higher degree of HP infection. This tendency was maximum in relation to bifidobacteria — 6.1-1.57-1.09 x 10⁸ CFU/g, with a difference between the maximum (I degree) and minimum (III degree) 5.59 times. A similar relationship, but much less pronounced, was observed for Lactobacterium spp. and E. coli. As the severity of HP infiltration of the gastric mucosa increases, there is a decrease in the concentration of the above microorganisms in the parietal mucin - the correlation coefficient is r = -0.74. Discussion. Since the mucous microflora is more stable and functionally significant than the cavity microflora, the data obtained allow us to consider Helicobacter pylori as an etiological factor of colonic dysbiosis. Conclusion. The probability of excessive growth of conditionally pathogenic fraction of intestinal parietal microflora has a direct dependence on the severity of Helicobacter pylori infestation, being maximum at degree III contamination. Content of bifidobacteria, lactobacilli and typical E. coli strains in the intestinal biotope of the large intestine is inversely related to the degree of histological activity of HP-associated gastritis.

scholarly journals Gallstones and Concomitant GastricHelicobacter pyloriInfection

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Wafi Attaallah ◽  
Nese Yener ◽  
M. Umit Ugurlu ◽  
Manuk Manukyan ◽  
Ebru Asmaz ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Gallstone Disease ◽  
Rapid Urease Test ◽  
Immunohistochemical Stain ◽  
Gallbladder Mucosa ◽  
Urease Test ◽  
Symptomatic Gallstones ◽  
H Pylori ◽  
Symptomatic Gallstone Disease

Background. The association of gallstones withHelicobacter pylorihas been investigated but not clearly demonstrated. In this study, the presence ofH. pyloriin the gallbladder mucosa of patients with symptomatic gallstones was investigated.Method. Ninety-four consecutive patients with symptomatic gallstone disease were enrolled for the study. Gastroscopy and gastricH. pyloriurease test were done before cholecystectomy to all patients who accepted. After cholecystectomy, the gallbladder tissue was investigated in terms ofH. pyloriby urease test, Giemsa, and immunohistochemical stain.Results. Overall 35 patients (37%) gallbladder mucosa tested positive forH. pyloriwith any of the three tests. Correlation of the three tests Giemsa, IHC, and rapid urease test was significant(rs: 0590,P>0.001). Rapid urease test was positive in the gastric mucosa in 47 (58.7%) patients, and it was positive in the gallbladder mucosa in 21 patients (22%). In 15 patients both gastric and gallbladder tested positive with the urease test. There was significant correlation of rapid urease test in both of gallbladder and gastric mucosa(P=0.0001).Conclusion. Study demonstrates the presence ofH. pyloriin the gallbladders of 37% of patients with symptomatic gallstones.

EFFICACY OF RACM REGIMEN ON HELICOBACTER PYLORI ERADICATION IN PATIENTS OF CHRONIC GASTRITIS

2016 ◽  
pp. 88-93
Author(s):  
Thi Hoai Thai ◽  
Van Huy Tran
Keyword(s):  
Helicobacter Pylori ◽  
Side Effects ◽  
Chronic Gastritis ◽  
Eradication Rate ◽  
Statistical Significance ◽  
Rapid Urease Test ◽  
Antibiotic Resistant ◽  
Helicobacter Pylori Eradication ◽  
Urease Test ◽  
H Pylori

Background: H. pylori eradication still remains a challenge to clinicians, especially with the increasing antibiotic-resistant H. pylori. Concomitant therapy showed effective, even in some multiresistant population, but data in Vietnam is still very limited. The study ''Study of Helicobacter pylori eradication with RACM regimen in chronic gastritis patients at Da Nang Hospital from 1/4/2014 to 30/6/2015, is aimed at: (1) Evaluating the results of Helicobacter pylori eradication of Amoxicillin-Clarithromycin-Rabeprazole-Metronidazole therapy for 14 days.(2) Assessing some side effects of this regimen.Method: prospective, consisting of 83 patients examined and treated in Danang hospital from1/ 4/2014 to 30/6/2015, H.pylori was tested by rapid Urease test; H.pylori positive patients received RACM for 14 days. Results: H.pylori eradication rate was 83.1%. H. pylori eradication rates in different locations: antrum 63.8%, higher than corpus (17.4%), antrum and corpus (18.8%), with statistical significance at p<0.05. Common side effects was nausea (27.7%), diarrhea (19.3%). Abdominal pain, lightheadedness, dizziness, insomnia, headache account for low percentage: 8%; 6%; 3,6% and 2.4% respectively. Conclusion: The effect of 14 day RACM regimen for H. pylori eradication was 83.1%, common side effects are nausea (27.7%), diarrhea (19.3%). Key words: chronic gastritis;H. pylori; eradication of H. pylori(ITT); RACM regimen.

CHRONIC HELICOBACTER PYLORI GASTRITIS: THE ERADICATION EFFICACY&NBSP;OF THE&NBSP;BISMUTH-CONTAINING QUADRUPLE REGIMEN (EBMT)

2016 ◽  
pp. 149-158
Author(s):  
Ngoc Quy Hue Dang ◽  
Van Huy Tran ◽  
Thanh Hai Nguyen
Keyword(s):  
Helicobacter Pylori ◽  
Side Effects ◽  
Chronic Gastritis ◽  
Medication Compliance ◽  
Compliance Rate ◽  
Eradication Therapy ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori ◽  
Helicobacter Pylori Gastritis

Background: there has not been yet any research on the effectiveness of H. pylori eradication of bismuth-containing quadruple regimen on chronic gastritis patients in our country. Objective: to evaluate H. pylori eradication rate of bismuth-containing quadruple regimen according to intention to treat (ITT), per protocol (PP) analysis, the rate of side effects and medication compliance. Subjects and Methods: from March 2014 to January 2016 we used bismuth-containing quadruple regimen (EBMT) 10 days for H. pylori eradication therapy for 166 chronic gastritis patients diagnosed based on clinical, endoscopic, rapid urease test, histology and culture. Patients were evaluated side effects and medication compliance at the end of treatment (day 11-14). To assess the eradication, repeating endoscopy with both rapid urease test and histological examination were performed at 4 to 8 weeks after stopping treatment course. Results: H. pylori eradication rates on ITT and PP analysis overall, for naïve patients, after one and more two eradication failures were respectively: 80.72-89.33%, 79.51-90.65%, 91.67-91.67% and 75.00-78.95%. Medication adherence rate was 96.99%. The rates of patients experiencing moderate, severe and very severe side effects were: 19.88%, 0.60% and 1.81%. Conclusion: the EBMT 10-day regimen attained high eradication rates in chronic gastritis patients with rare serious side effects and the high compliance rate. We should apply bismuth-containing quadruple regimen in H. pylori eradication therapy for naïve patients or after one eradication failure. Key words: bismuth-containing quadruple regimen, EBMT, eradication, chronic Helicobacter pylori gastritis

scholarly journals Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children over a period of 10 years

2007 ◽  
Vol 56 (8) ◽  
pp. 1081-1085 ◽  
Author(s):  
Lyudmila Boyanova ◽  
Elena Lazarova ◽  
Christo Jelev ◽  
Galina Gergova ◽  
Ivan Mitov
Keyword(s):  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Living Environment ◽  
Rural Children ◽  
Rapid Urease Test ◽  
Infection Prevalence ◽  
Helicobacter Heilmannii ◽  
Urease Test ◽  
H Pylori ◽  
The Impact

The aims of the study were to evaluate the incidence of Helicobacter pylori and Helicobacter heilmannii in untreated Bulgarian children from 1996 to 2006, to analyse the performance of diagnostic tests, and to look at H. pylori density in specimens by culture. Antral specimens from children with chronic gastritis (n=513), peptic ulcers (n=54) and other diseases (n=91) were evaluated by direct Gram staining (DGS), in-house rapid urease test (RUT) and culture. The living environment and semi-quantitative H. pylori density were assessed in 188 and 328 children, respectively. H. pylori infection was found in children with ulcers (77.8  %), chronic gastritis (64.5  %) and other diseases (36.3  %). Half (51.4  %) of patients aged 1–5 years and 77.4  % of those aged 16–17 years were H. pylori-positive. Of all children, 328 (49.8  %) showed positive DGS, 184 (28  %) had a positive RUT, and 386 (58.7  %) were culture-positive. Unlike gastric mucus specimens, frozen biopsy specimens provided reliable diagnosis. H. heilmannii was observed in two (0.3  %) children. High H. pylori density (growth into all quadrants of plates) was found in 18  % of 328 children evaluated, involving 31  % of ulcer and 16.7  % of non-ulcer patients. H. pylori infection was more common in rural children with chronic gastritis (91.3  %) than in the remainder (66.7  %). In conclusion, H. pylori infection was common in symptomatic Bulgarian children. The infection prevalence was >77  % in patients aged 16–17 years, in children with a duodenal ulcer, and in rural patients. H. heilmannii infection was uncommon. The performance of the bacterial culture was good. The impact of H. pylori density on the clinical expression and eradication of the infection requires further evaluation. The results highlight the need for routine H. pylori diagnosis in rural children with chronic gastritis.

scholarly journals Prevalence and genotyping of Helicobacter pylori in endoscopic biopsy samples from a Chinese population

2019 ◽  
Vol 43 (1) ◽  
pp. 21-28
Author(s):  
Hao Yu ◽  
Yingjia Mao ◽  
Lijie Cong ◽  
Zhiyong Wang ◽  
Hua Zhang ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Real Time ◽  
Chinese Population ◽  
Rflp Analysis ◽  
Endoscopic Biopsy ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori

Abstract Background: Helicobacter pylori inhabit the gastric mucosa of humans and are associated with several gastrointestinal diseases which include gastric cancer, peptic ulcer, chronic gastritis and gastric mucosa-associated lymphoid tissue lymphoma. Helicobacter pylori exhibit a high degree of genetic variability and are associated with its epidemiological, pathological characteristics and dynamics of transmission. The objective of the study was to determine the prevalence and genetic heterogeneity of H. pylori isolated from endoscopic biopsy samples from a Chinese population. Methods: Gastric biopsy samples from 86 patients (males, 55; females, 35) who presented to the endoscopic section for various gastrointestinal abnormalities were collected. The samples were subjected to a real-time polymerase chain reaction (PCR) and microbial culture for the isolation of H. pylori. Further, the isolates were subjected to randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) analysis. Results: Of the 86 gastric biopsy samples, 61 (70.9%) samples were positive for rapid urease test and 37 (43%) samples (28 from male and nine from female) grew H. pylori. Among the biopsy samples subjected to real-time PCR, 39 (45.3%) samples were found to be positive for H. pylori. The RAPD analysis yielded 15 different patterns (four to 17 different sized fragments per strain). The phylogenetic analysis of RAPD yielded 22 clusters at a similarity level ranging from 63% to 100%. RFLP analysis yielded nine different patterns (two to six different sized fragments per strain). Two major restriction patterns were identified, of which 14 (37.8%) strains forms the most common pattern (genotype I) followed by five (13.5%, genotype II) strains with an intra-strain similarity of 100%. Conclusions: The overall prevalence of H. pylori was 45.3%. Despite reports on the declining trend in the prevalence of H. pylori infections, our prevalence rate was still higher than those reported from other developed countries. However, further studies involving a large sample size and covering more regions of China is highly warranted.

STUDY OF HELICOBACTER PYLORI ERADICATON RATE OF BISMUTH-CONTAINING QUADRUPLE REGIMEN IN PATENTS WITH CHRONIC GASTRITS

2016 ◽  
pp. 31-35
Author(s):  
Van Huy Tran ◽  
Thi Minh Trieu Nguyen
Keyword(s):  
Helicobacter Pylori ◽  
Side Effects ◽  
Chronic Gastritis ◽  
Eradication Rate ◽  
High Rate ◽  
Rapid Urease Test ◽  
Good Tolerance ◽  
Central Vietnam ◽  
Urease Test ◽  
H Pylori

Background: Quadruple therapy with bismuth was considered as a promising regimen in the eradication of Helicobacter pylori in the population with clarithromycine- resistant high rate, but data in Vietnam was still limited. This study is aimed at determining the eradication rate of quadruple regimen and its side effects in the patients with chronic gastritis. Patients and methods: 98 patients with chronic gastritis histologically diagnosed and postive with rapid urease test were enrolled. Patients received Rabeprazole 20 mg bid, Pepto bismol 2tab bid, tetracyclin 1000 mg bid and Metronidazole 550 mg bid for 10 days. Results: Eradication rates of quadruple regimens with bismuth were 91.8% in ITT analysis and 94.7% in PP analysis. Rate of compliance was 96.9%. The side effects were minor and transient. Conclusion: Quadruple regimen with bismuth showed a high rate of H. pylori eradication and a good tolerance in patients with chronic gastritis in central Vietnam. Key words: Helicobacter pylori, quadruple regimen, bismuth

A Clinical Significance of Helicobacter Pylori Infection in Hemophilic Children with Gastrointestinal Hemorrhage.

Blood ◽  
2009 ◽  
Vol 114 (22) ◽  
pp. 4442-4442
Author(s):  
Ji Yoon Kim ◽  
Yong-Mook Choi ◽  
Kun Soo Lee
Keyword(s):  
Duodenal Ulcer ◽  
Helicobacter Pylori ◽  
Chronic Gastritis ◽  
Gastrointestinal Hemorrhage ◽  
Conflicts Of Interest ◽  
Helicobacter Pylori Infection ◽  
Rapid Urease Test ◽  
Urease Test ◽  
Life Threatening ◽  
H Pylori

Abstract Abstract 4442 Purpose For hemophiliacs gastrointestinal hemorrhage is a life-threatening complication and can be caused by the Helicobacter pylori infection. In this study, the prevalence of H. pylori infection among child hemophiliacs with gastrointestinal hemorrhage and the recurrence rate after eradication H. pylori treatment was investigated. Patients and Methods Seven children with hemophilia A with hematemesis (age, 5.3∼17.0 year) were evaluated for the causes of gastrointestinal hemorrhage and the detection of H. pylori. Gastroendoscopy was done to find the bleeding focus and for further evaluation including rapid urease test and mucosal biopsy. Result: Four patients had dyspepsia and abdominal pain for several weeks or months prior to hematemesis. Three patients did not show any signs of bleeding. From gastroendoscopy, four patients were diagnosed as duodenal ulcer, one as H. pylori associated chronic gastritis and one as hemorrhagic gastritis. One patient showing a normal finding was diagnosed with adenoid hemorrhage after nasopharyngoscopy. H. pylori infection was found in four of six patients with GI bleeding (3, duodenal ulcer; 1, H. pylori associated chronic gastritis). The patients with H. pylori infection had an eradication treatment of triple therapy and no recurrence happened. Conclusion In child hemophiliacs, H. pylori should also be considered as an important cause of gastrointestinal hemorrhage. The recurrence of the infection and gastrointestinal hemorrhage can be prevented with eradication of H. pylori. Screening test for H. pylori would be needed in child hemophiliacs in endemic area. Disclosures: No relevant conflicts of interest to declare.

Prevalence and genotyping of Helicobacter pylori in endoscopic biopsy samples from a Chinese population

2018 ◽  
Vol 0 (0) ◽  
Author(s):  
Hao Yu ◽  
Yingjia Mao ◽  
Lijie Cong ◽  
Zhiyong Wang ◽  
Hua Zhang ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Mucosa ◽  
Real Time ◽  
Chinese Population ◽  
Rflp Analysis ◽  
Endoscopic Biopsy ◽  
Gastric Biopsy ◽  
Rapid Urease Test ◽  
Urease Test ◽  
H Pylori

AbstractBackground:Helicobacter pylori inhabit the gastric mucosa of humans and are associated with several gastrointestinal diseases which include gastric cancer, peptic ulcer, chronic gastritis and gastric mucosa-associated lymphoid tissue lymphoma. Helicobacter pylori exhibit a high degree of genetic variability and are associated with its epidemiological, pathological characteristics and dynamics of transmission. The objective of the study was to determine the prevalence and genetic heterogeneity of H. pylori isolated from endoscopic biopsy samples from a Chinese population.Methods:Gastric biopsy samples from 86 patients (males, 55; females, 35) who presented to the endoscopic section for various gastrointestinal abnormalities were collected. The samples were subjected to a real-time polymerase chain reaction (PCR) and microbial culture for the isolation of H. pylori. Further, the isolates were subjected to randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) analysis.Results:Of the 86 gastric biopsy samples, 61 (70.9%) samples were positive for rapid urease test and 37 (43%) samples (28 from male and nine from female) grew H. pylori. Among the biopsy samples subjected to real-time PCR, 39 (45.3%) samples were found to be positive for H. pylori. The RAPD analysis yielded 15 different patterns (four to 17 different sized fragments per strain). The phylogenetic analysis of RAPD yielded 22 clusters at a similarity level ranging from 63% to 100%. RFLP analysis yielded nine different patterns (two to six different sized fragments per strain). Two major restriction patterns were identified, of which 14 (37.8%) strains forms the most common pattern (genotype I) followed by five (13.5%, genotype II) strains with an intra-strain similarity of 100%.Conclusions:The overall prevalence of H. pylori was 45.3%. Despite reports on the declining trend in the prevalence of H. pylori infections, our prevalence rate was still higher than those reported from other developed countries. However, further studies involving a large sample size and covering more regions of China is highly warranted.

Frequency of rdx A Deletion Mutation Conferring Metronidazole Resistance in Helicobacter pylori

2020 ◽  
Vol 29 (3) ◽  
pp. 59-64
Author(s):  
Hanaa M. El Maghraby ◽  
Samar Mohaseb
Keyword(s):  
Helicobacter Pylori ◽  
Epigastric Pain ◽  
Deletion Mutation ◽  
Rapid Urease Test ◽  
Rrna Gene ◽  
University Hospitals ◽  
Metronidazole Resistance ◽  
Urease Test ◽  
Gastric Biopsies ◽  
H Pylori

Background: Metronidazole is one of the antimicrobial drugs that can be used in combination with other drugs for eradication of Helicobacter pylori (H. pylori).Unfortunately, metronidazole resistance in H. plori is an increasing health problem which may be attributed to inactivation of many genes as rdx A gene. Objective: To determine the frequency of rdx A deletion mutation in H. pylori detected in infected patients attending at the Gastroenterology Unit, Zagazig University Hospitals. Methodology: Two gastric biopsies were taken from each enrolled patient by endoscopy. H.pylori detection was done by rapid urease test and polymerase chain reaction (PCR) amplification of 16S rRNA gene. Deletion mutation in rdx A gene was detected by conventional PCR. Results: Out of 134 doubled gastric biopsies obtained from 134 patients, 52.2% were positive for H. pylori. Epigastric pain, vomiting and gastritis were significantly associated with detection of H. pylori infection (p˂ 0.05). Deletion mutation of rdx A gene was detected in 28.6% of H. pylori positive specimens obtained from infected patients. Conclusion: Deletion mutation of rdx A gene is a frequent determinant of rdx A inactivation conferring metronidazole resistance among H. pylori.

scholarly journals Incidence of Gastric Metaplasia and Helicobacter pylori Infection in Duodenal Bulb – With Specific Reference to Patients With Duodenal Ulcers

1999 ◽  
Vol 6 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Minoru Kawaguchi ◽  
Toshihiko Saito
Keyword(s):  
Helicobacter Pylori ◽  
Duodenal Bulb ◽  
Mucosal Injury ◽  
Transitional Zone ◽  
Specific Reference ◽  
Rapid Urease Test ◽  
Duodenal Ulcers ◽  
Gastric Metaplasia ◽  
Urease Test ◽  
H Pylori

We determined the incidence of gastric metaplasia in the duodenal bulb of duodenal ulcer patients and the Helicobacter pylori (H. pylori) infection rate at sites with gastric metaplasia. Biopsy of the duodenal bulb showed the presence of gastric metaplasia in 61 of 86 patients (71%) overall and in 18 of 47 patients (38.3%) who had gastrectomy at an early gastric cancer. The histological diagnosis of H. pylori infection showed good agreement (83.3%) with the result of the rapid urease test, indicating that H. pylori occurs in regions with gastric metaplasia. This finding suggests that H. pylori infects gastric metaplasia in the duodenal bulb, causing mucosal injury, which is then transformed into duodenal ulcers. The exact mechanism by which gastric metaplasia is caused is unknown, but it is believed to occur in the transitional zone in the duodenal mucosa.

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