Preparation, Preliminary Characterization and Antiangiogenic Activities of Polysaccharides from Pomegranate Peels

In the present study, the crude polysaccharides from pomegranate peels (CPP) were prepared by compound enzyme-extraction technology, and the response surface methodology was used to optimize the extraction parameters. Box–Behnken design (BBD) was applied to estimate the effects of extraction temperature, extraction pH, and dosage of compound enzyme on the yield of CPP. A mathematical model with high fitness was obtained. Extraction temperature, extraction pH, and dosage of compound enzyme exhibited independent and interactive effects on CPP yield. CPP were purified by macroporous resin HP-20 and Sephadex G-100 column chromatography to afford purified fraction of CPP-2. The relative molecular weight of CPP-2 was 93.5 kDa. In CPP-2, and FT-IR spectra showed that the main components among CPP-2 may be pectic polysaccharides.The effect of CPP-2 on angiogenesis was measured in vivo by using the chick embryo chorioallantoic membrane (CAM) assay. The results demonstrated that CPP-2 suppressed angiogenesis in chicken embryos.

Download Full-text

The Chick Embryo Chorioallantoic Membrane as an In Vivo Assay to Study Antiangiogenesis

Pharmaceuticals ◽

10.3390/ph3030482 ◽

2010 ◽

Vol 3 (3) ◽

pp. 482-513 ◽

Cited By ~ 22

Author(s):

Domenico Ribatti

Keyword(s):

Chick Embryo ◽

Chorioallantoic Membrane ◽

In Vivo Assay ◽

Chick Embryo Chorioallantoic Membrane

Download Full-text

The Chick Embryo Chorioallantoic Membrane as an in vivo Wound Healing Model

Pathology - Research and Practice ◽

10.1016/s0344-0338(96)80050-1 ◽

1996 ◽

Vol 192 (10) ◽

pp. 1068-1076 ◽

Cited By ~ 15

Author(s):

D. Ribatti ◽

A. Vacca ◽

G. Ranieri ◽

S. Sorino ◽

L. Roncali

Keyword(s):

Wound Healing ◽

Chick Embryo ◽

Chorioallantoic Membrane ◽

Chick Embryo Chorioallantoic Membrane ◽

Wound Healing Model ◽

Healing Model

Download Full-text

The chick embryo chorioallantoic membrane as an in vivo experimental model to study multiple myeloma

Chick Chorioallantoic Membrane Model and Precision Cancer Therapy - The Enzymes ◽

10.1016/bs.enz.2019.08.006 ◽

2019 ◽

pp. 23-35

Author(s):

Domenico Ribatti ◽

Roberto Tamma

Keyword(s):

Multiple Myeloma ◽

Chick Embryo ◽

Experimental Model ◽

Chorioallantoic Membrane ◽

Chick Embryo Chorioallantoic Membrane

Download Full-text

In vivo invasion of modified chorioallantoic membrane by tumor cells: the role of cell surface-bound urokinase.

The Journal of Cell Biology ◽

10.1083/jcb.107.6.2437 ◽

1988 ◽

Vol 107 (6) ◽

pp. 2437-2445 ◽

Cited By ~ 169

Author(s):

L Ossowski

Keyword(s):

Cell Surface ◽

Plasminogen Activator ◽

Tumor Cells ◽

Chorioallantoic Membrane ◽

Human Tumor ◽

Chick Embryo Chorioallantoic Membrane ◽

Catalytically Active ◽

Surface Receptor

The ability of the chick embryo chorioallantoic membrane (CAM) to withstand invasion by tumor cells can be intentionally compromised by altering its morphological integrity. Using a newly developed quantitative assay of invasion we showed that intact CAMs were completely resistant to invasion by tumor cells, wounded CAMs did not pose a barrier to penetration, and CAMs that were wounded and then allowed to reseal displayed partial susceptibility to invasion. The invasion of resealed CAMs required catalytically active plasminogen activator (PA) of the urokinase type (uPA); the invasive efficiency of tumor cells was reduced by 75% when tumor uPA activity or tumor uPA production was inhibited. The invasive ability of human tumor cells, which have surface uPA receptors but which do not produce the enzyme, could be augmented by saturating their receptors with exogenous uPA. The mere stimulation of either uPA or tissue plasminogen activator production, in absence of binding to cell receptors, did not result in an enhancement of invasiveness. These findings suggest that the increased invasive potential of tumor cells is correlated with cell surface-associated proteolytic activity stemming from the interaction between uPA and its surface receptor.

Download Full-text