ISOLATION AND IDENTIFICATION OF FUNGI FROM SUBMERGED PLANTS DEBRIS IN AQUATIC HABITATS IN MISAN PROVINCE

Forty-eight taxa of saprophytes fungi were isolated and identified from submerged plant debris samples collected from different sites in Misan province, southern Iraq, which are Maymouna, Al-Salam, Majar Al-Kabir, and Amara. Among them, 24 species belonged to Ascomycota (six of which are sexual state), 19 species to hyphomycetes (anamorphic fungi), 4 to Zygomycota, and one to Oomycetes. Six species were isolated and newly recorded from Iraq, which are Aniptodera margaration, Cirrenalia iberica, Cordana lignicola, Cordana verruculosa, Pseudoacrodictys appendiculata, and Scytalidium thermophilum. However, in the evaluation of both moist chamber and direct culture methods, 34 species were isolated by the first method and 27 species by the second method, meantime, 13 species (9 belonged to Ascomycota, 2 to hyphomycetes, and 2 to Zygomycetes) were recovered by both methods. Aspergillus terrus was appeared in highest frequency and occurrence (11.76%, 42.55%, respectively), followed by Aspergillus horti (10%, 36.17%, respectively), and then A. niger with a frequency and occurrence of 5.29% and 19.14%, respectively, while 17 species were appeared lowest frequency and occurrence to reach 0.58%, 2.12%, respectively, for all. One hundred and seven isolates have been recovered from all study sites. Meantime, sixty-four isolates have been reported from Majar Al-Kabir, as compared with other sites, followed by Amara (43 isolates), while 39 isolates have been isolated from the Maymouna, and 24 isolates from Al-Salam. The biodiversity of fungi isolated from submerged plant debris was compared with previous studies. Brief descriptions of the new recorded fungi were given.

Download Full-text

Isolation and identification of fungi associated with the spoilage of sweet orange (Citrus sinensis L) and banana (Musa sapientum L) in Sokoto Metropolis

Journal of Applied Biotechnology & Bioengineering ◽

10.15406/jabb.2018.05.00135 ◽

2018 ◽

Vol 5 (3) ◽

Author(s):

Muhammad AS ◽

Mohammed IU ◽

Ameh M ◽

Bello I ◽

Haliru BS ◽

...

Keyword(s):

Citrus Sinensis ◽

Sweet Orange ◽

Isolation And Identification ◽

Musa Sapientum ◽

Identification Of Fungi

Download Full-text

Selection, isolation, and identification of fungi for bioherbicide production

Brazilian Journal of Microbiology ◽

10.1016/j.bjm.2016.09.004 ◽

2017 ◽

Vol 48 (1) ◽

pp. 101-108 ◽

Cited By ~ 24

Author(s):

Angélica Rossana Castro de Souza ◽

Daiana Bortoluzzi Baldoni ◽

Jessica Lima ◽

Vitória Porto ◽

Camila Marcuz ◽

...

Keyword(s):

Isolation And Identification ◽

Identification Of Fungi

Download Full-text

Isolation and identification of fungi associated with some Libyan foods

African Journal of Food Science ◽

10.5897/ajfs2015.1318 ◽

2015 ◽

Vol 9 (7) ◽

pp. 406-410 ◽

Cited By ~ 2

Author(s):

Alkenz S ◽

A Sassi A ◽

S Abugnah Y ◽

B Alryani M

Keyword(s):

Isolation And Identification ◽

Identification Of Fungi

Download Full-text

Isolation and identification of fungi from apparently healthy and diseased Clarias gariepinus from freshwater in Zaria, Kaduna State, Nigeria

Microbiology Research International ◽

10.30918/mri.51.17.012 ◽

2017 ◽

Vol 5 (1) ◽

pp. 8-15

Author(s):

M. T. Salawudeen ◽

◽

H. M. Kazeem ◽

M. A. Raji ◽

S. J. Oniye ◽

...

Keyword(s):

Clarias Gariepinus ◽

Isolation And Identification ◽

Identification Of Fungi ◽

Apparently Healthy

Download Full-text

Rapid Detection of Campylobacter coli, C. jejuni, and Salmonella enterica on Poultry Carcasses by Using PCR-Enzyme-Linked Immunosorbent Assay

Applied and Environmental Microbiology ◽

10.1128/aem.69.6.3492-3499.2003 ◽

2003 ◽

Vol 69 (6) ◽

pp. 3492-3499 ◽

Cited By ~ 48

Author(s):

Yang Hong ◽

Mark E. Berrang ◽

Tongrui Liu ◽

Charles L. Hofacre ◽

Susan Sanchez ◽

...

Keyword(s):

Immunosorbent Assay ◽

Salmonella Enterica ◽

Rapid Detection ◽

Diarrheal Disease ◽

False Negative ◽

Enzyme Linked Immunosorbent Assay ◽

Campylobacter Coli ◽

Culture Methods ◽

Isolation And Identification ◽

Serological Tests

ABSTRACT Contamination of retail poultry by Campylobacter spp. and Salmonella enterica is a significant source of human diarrheal disease. Isolation and identification of these microorganisms require a series of biochemical and serological tests. In this study, Campylobacter ceuE and Salmonella invA genes were used to design probes in PCR-enzyme-linked immunosorbent assay (ELISA), as an alternative to conventional bacteriological methodology, for the rapid detection of Campylobacter jejuni, Campylobacter coli, and S. enterica from poultry samples. With PCR-ELISA (40 cycles), the detection limits for Salmonella and Campylobacter were 2 Ã¯Â¿Â½ 102 and 4 Ã¯Â¿Â½ 101 CFU/ml, respectively. ELISA increased the sensitivity of the conventional PCR method by 100- to 1,000-fold. DNA was extracted from carcass rinses and tetrathionate enrichments and used in PCR-ELISA for the detection of Campylobacter and S. enterica, respectively. With PCR-ELISA, Salmonella was detected in 20 of 120 (17%) chicken carcass rinses examined, without the inclusion of an enrichment step. Significant correlation was observed between PCR-ELISA and cultural methods (kappa = 0.83; chi-square test, P < 0.001) with only one false negative (1.67%) and four false positives (6.67%) when PCR-ELISA was used to screen 60 tetrathionate enrichment cultures for Salmonella. With PCR-ELISA, we observed a positive correlation between the ELISA absorbance (optical density at 405 nm) and the campylobacter cell number in carcass rinse, as determined by standard culture methods. Overall, PCR-ELISA is a rapid and cost-effective approach for the detection and enumeration of Salmonella and Campylobacter bacteria on poultry.

Download Full-text

#38: Bacteremia Analysis of Three Hospitals in Hispaniola Island

Journal of the Pediatric Infectious Diseases Society ◽

10.1093/jpids/piab031.046 ◽

2021 ◽

Vol 10 (Supplement_2) ◽

pp. S19-S19

Author(s):

Johanny Contreras ◽

Karina Rivera ◽

María Castillo ◽

Genara Santana ◽

María Dolores Gil ◽

...

Keyword(s):

Lymphoblastic Leukemia ◽

Mucosal Barrier ◽

Infection Prevention And Control ◽

Coagulase Negative Staphylococcus ◽

Case Definitions ◽

Culture Methods ◽

Isolation And Identification ◽

Infection Surveillance ◽

Prophylactic Measures ◽

Healthcare Associated

Abstract Background In October 2018, the Hispaniola Project was initiated to build local expertise in infection care and prevention at three pediatric oncology units (POUs) in Haiti and the Dominican Republic. Surveillance of healthcare-associated infections (HAI) was a central aim. Severe and prolonged neutropenia is a frequent risk factor for infections in oncology patients. Among HAIs, bacteremia is one of the most serious; bacteremia requires timely isolation and identification of the offending microorganism and the antimicrobial susceptibility. These diagnostic interventions allow informed therapeutic and prophylactic measures. Here, we report our experience in bacteremia in these 3 POUs. Methods We conducted prospective infection surveillance of all patients admitted to three POUs in Hispaniola Island. Blood culture methods followed standard national procedures. We used the 2018 US Centers for Disease Control National Healthcare Safety Network case definitions for primary laboratory-confirmed bloodstream infections (LCBI), and we categorized infections as healthcare-associated or present on admission (POA). We reviewed data collected from January 2019 to December 2020 and used descriptive statistics to report our results. Results Our review identified 66 LCBIs with an overall rate of 3.52 infections per 1000 patient-days. Of these, 40 (61%) were healthcare-associated, and 26 were POA. The majority (41, 62%) of patients were undergoing chemotherapy at the time of the infection, with induction being the most common phase (23). The most common oncologic diagnosis was acute lymphoblastic leukemia (43, 65%), followed by solid tumor (12, 18%). Fifty-three (80%) of the infections met the LCBI-1 criteria, with the other 13 categorized as LBCI-2. Of the 53 LCBI-1, 7 (13%) were considered related to mucosal barrier injury (MBI-LCBI 1 definition). The most commonly identified organisms were Klebsiella spp. (13, 19%) and coagulase-negative Staphylococcus (13, 19%). Antibiotic resistance was observed in many of the identified pathogens, with nearly half (25, 44%) of the 57 bacterial isolates having any resistance and a quarter (14, 25%) with resistance to multiple classes, including cephalosporins, fluoroquinolones, and aminoglycosides. Eleven (17%) patients were admitted to the Intensive Care Unit as a result of the LCBI. Thirteen deaths were recorded among the patients with LCBIs, with 6 (46%) associated with the HAI and 7 (54%) related to disease progression. Conclusions Our findings demonstrate that resistant pathogens were frequent among the LCBI isolates. Our preliminary results are guiding clinical management to be vigilant in our care of patients at high risk for bacteremia and poor clinical response by initiating more effective antimicrobials sooner. Importantly, reviewing reasons for antimicrobial resistance and implementing best antimicrobial use practices will protect our fragile antibiotic arsenal. Infection surveillance programs, such as ours, and other initiatives which promote infection prevention and control in POU will increase the quality of care for these vulnerable patients.

Download Full-text

Isolation and Identification of Campylobacter spp. from Poultry and Poultry By-Products in Tunisia by Conventional Culture Method and Multiplex Real-Time PCR

Journal of Food Protection ◽

10.4315/0362-028x.jfp-16-321 ◽

2017 ◽

Vol 80 (10) ◽

pp. 1623-1627 ◽

Cited By ~ 3

Author(s):

Hela Jribi ◽

Hanen Sellami ◽

Siala Mariam ◽

Salma Smaoui ◽

Asma Ghorbel ◽

...

Keyword(s):

Real Time ◽

Real Time Pcr ◽

Poultry Meat ◽

Culture Methods ◽

Isolation And Identification ◽

Conventional Culture ◽

Multiplex Pcr Assay ◽

Thermophilic Campylobacter ◽

By Products ◽

Campylobacter Spp

ABSTRACT Thermophilic Campylobacter spp. are one of the primary causes of bacterial human diarrhea. The consumption of poultry meats, by-products, or both is suspected to be a major cause of human campylobacteriosis. The aims of this study were to determine the prevalence of thermophilic Campylobacter spp. in fresh poultry meat and poultry by-products by conventional culture methods and to confirm Campylobacter jejuni and Campylobacter coli isolates by using the multiplex PCR assay. Two hundred fifty fresh poultry samples were collected from a variety of supermarkets and slaughterhouses located in Sfax, Tunisia, including chicken (n =149) and turkey (n =101). The samples were analyzed using conventional microbiological examinations according to the 2006 International Organization for Standardization method (ISO 10272-1) for Campylobacter spp. Concurrently, a real-time PCR was used for identification of C. jejuni and C. coli. Of the 250 samples of poultry meat and poultry by-products, 25.6% (n = 64) were contaminated with Campylobacter spp. The highest prevalence of Campylobacter spp. was found in chicken meat (26.8%) followed by turkey meat (23.7%). Among the different products, poultry breasts showed the highest contamination (36.6%) followed by poultry by-products (30%), poultry wings (28%) and poultry legs (26%) showed the lowest contamination, and no contamination was found on neck skin. Of the 64 thermophilic Campylobacter isolates, C. jejuni (59.7%) was the most frequently isolated species and 10.9% of the isolates were identified as C. coli. All of the 64 Campylobacter isolates identified by the conventional culture methods were further confirmed by PCR. The seasonal peak of Campylobacter spp. contamination was in the warm seasons (spring and summer). The study concluded that high proportions of poultry meat and poultry by-products marketed in Tunisia are contaminated by Campylobacter spp. Furthermore, to ensure food safety, poultry meats must be properly cooked before consuming.

Download Full-text