Isolation and Identification of Fungi from Spices and Medicinal Plants

Mycological studies on Fungi in apparently diseased Sweet Orange ( Citrus sinensis) and Banana ( Musa sapientum) sampled from various points in Kara market in Sokoto Metropolis was carried out between August and September. The samples were surfaced sterilised with ethanol and the homogenates were cultured on Potato Dextrose Agar (PDA) and incubated aerobically at room temperature for 7 days at 30C. The pure cultures obtained were identified morphologically and microscopically. The investigation revealed that the samples were infected with several fungi species. The most predominant Fungi isolated from Sweet Orange were Cladosporium spp (40%), Fusarium spp (30%), Alternaria spp ( 20%), and Chrysonilia spp (10%) while the most predominant Fungi isolated from Banana were Fusarium spp (50%), Mucor spp ( 30%) and Rhizopus spp (20%). The pathogenecity test results show that Cladosporium spp and Fusarium spp were the most active in sweet orange with rot length of 74 mm and 70 mm respectively and the least active fungi were Alternaria spp and Chrysonilia spp with rot lengths of 52 mm and 48 mm respectively. Furthermore, Fusarium spp and Mucor spp were the most active in banana with rot lengths of 84 mm and 75 mm respectively and the least active fungus was Rhizopus spp with rot lengths of 54 mm.

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Isolation and Identification of Fungi from fast food restaurants in Langa Bazar

International Journal of Environment Agriculture and Biotechnology ◽

10.22161/ijeab/2.4.10 ◽

2017 ◽

Vol 2 (4) ◽

pp. 1517-1522 ◽

Cited By ~ 2

Author(s):

Sewgil Saaduldeen Anwer ◽

Gazang A. Ali ◽

Chra Z. Hamadamin ◽

Hanan Y. Jaafar

Keyword(s):

Fast Food ◽

Isolation And Identification ◽

Identification Of Fungi ◽

Fast Food Restaurants

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ISOLATION AND IDENTIFICATION OF FUNGI ASSOCIATED WITH COMPOSTING PROCESS OF MUNICIPAL BIOSOLID WASTE

Vietnam Journal of Biotechnology ◽

10.15625/1811-4989/15/4/13421 ◽

2018 ◽

Vol 15 (4) ◽

pp. 763-770

Author(s):

Pham Thi Thu Hang ◽

Le Thi Quynh Tram ◽

Tran Phuong Anh ◽

Ho To Thi Khai Mui ◽

Dang Nguyen Thao Vi ◽

...

Keyword(s):

Large Scale ◽

Morphological Characteristics ◽

Degradation Process ◽

Isolation And Identification ◽

Fungal Strains ◽

Composting Process ◽

Rdna Sequences ◽

Identification Of Fungi ◽

18S Rdna Sequences ◽

Living Organisms

Organic waste is gradually degraded during composting process, producing carbon dioxide, water, heat, and humus, the relatively stable end product. The degradation process is carried out by living organisms, of which fungi appear to have the most important role since they break down tough debris (cellulose, lignin, and other resistant materials), enabling other microorganisms to continue the decomposition process. The objective of this study was to isolate and identify the fungi associated with large scale municipal biosolid waste composting process in Vietnam. In this study, we have isolated 10 morphologically different fungal strains from the composting materials, and classified based on morphological characteristics and 18S rDNA sequences. The results showed that these fungal strains belonged to four different genera, including Aspergillus, Penicillium, Monascus, and Trichoderma. The results would be a useful reference for further studies of diversity, and functions of fungi that involved in municipal biosolid waste composting process in Vietnam environmental conditions.

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Isolation and Identification of Fungi Associated With Rot of Watermelon Fruit in Sokoto Metropolitan, Sokoto State, Nigeria

International Journal of Pathogen Research ◽

10.9734/ijpr/2020/v5i430146 ◽

2020 ◽

pp. 78-83

Author(s):

Y. I. Alhaji ◽

S. Y. Lema ◽

J. Ibrahim ◽

A. Umar ◽

M. Garba

Keyword(s):

Public Health ◽

Foodborne Diseases ◽

Pathogenic Microorganisms ◽

Rhizopus Stolonifer ◽

Public Health Importance ◽

Isolation And Identification ◽

Standard Procedures ◽

Identification Of Fungi ◽

Fungal Organisms ◽

Careful Handling

Watermelon fruit contain high vitamins and minerals. Watermelon is susceptible to many pathogenic microorganisms that results to unpleasant odours and cause foodborne diseases. This study was conducted to isolate and identify fungi that are associated with rot of watermelon fruit in Sokoto metropolitan. The presence of fungal organisms was examined in five different locations within Sokoto metropolitan. Isolated fungus was identified morphologically, microscopically and using molecular standard procedures. Results showed that (3- Aspergillus is one gemus) 4 fungal genera were isolated and Identified. The isolated fungi were Asperqillus flavus, Aspergillus niger. Rhizopus stolonifer and Mucor spp. Fungi isolated from this study are of economical and public health importance. This study was carried out to examine the fungi associated with the rot of watermelon fruit in Sokoto metropolitan. Careful handling of watermelon fruit are recommended to ensure longer shelf-life, prevent spread of rot pathogens and keep fruit in the highest quality before consumption.

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