Extracellular Polymeric Substance (EPS) Degradation of Enterococcus Faecalis biofilm after irradiation with 405nm diode laser

2021 ◽  
pp. 3869-3873
Author(s):  
Kun Ismiyatin ◽  
Mochamad Mudjiono ◽  
Sri Kunarti ◽  
Maria Liliana Santoso ◽  
Dalhar Hakiki ◽  
...  
Keyword(s):  
Diode Laser ◽  
Enterococcus Faecalis ◽  
Extracellular Polymeric Substance ◽  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Negative Group ◽  
Scanning Microscope ◽  
Treatment Groups ◽  
Polymeric Substance

This research aimed to determine whether irradiation of 405 nm diode laser with chlorophyll as photosensitizer could degrade the extracellular polymeric substance (EPS) of Enterococcus faecalis (E. faecalis) biofilm. The material for this study needs 25 biofilm formed by E. faecalis was divided equally into five groups. The control negative group (C-) consisted of E. faecalis biofilm, the control positive group (C+) consisted of E. faecalis biofilm and chlorophyll photosensitizers, and the other three treatment group (T1, T2, T3) consisted of E. faecalis biofilm and chlorophyll photosensitizers. Each treatment groups were irradiated for 90 second (s) for T1 group, 105 s for T2 group, and 120 s for T3 group with 405nm diode laser. The degradation EPS of E. faecalis’ biofilm was determined using Confocal Laser Scanning Microscope (CLSM). Irradiation duration affected the degradation EPS of E. faecalis’ biofilm. Chlorophyll with 120 s laser irradiation showed significant degradation EPS of E. faecalis’ biofilm compared to other groups (p < 0.05). Irradiation of diode laser 405nm with chlorophyll photosensitizer 120 s could degrade EPS of E. faecalis biofilm up to 97.51%.

scholarly journals ROOT CEMENTUM INVASION AND ADHESION BY ENTEROCOCCUS FAECALIS CONFOCAL ANALYSIS.

2012 ◽  
Vol 02 (04) ◽  
pp. 44-49
Author(s):  
Rahul Halkai ◽  
Mithra N. Hegde ◽  
Kiran Halkai
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser ◽  
Control Group ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Group I ◽  
Group Ii

Abstract Aims to know ability of Enterococcus faecalis invasion and adhesion into root cementum. Methodology Forty single rooted human intact teeth were selected, group I with 20 teeth as control group with no access opening and apical seal with varnish. Group II with 20 teeth, after access opening and canal debridement, all the samples were subjected for gamma sterilization to ensure complete absence of microorganisms, and then exposed to Enterococcus faecalis broth, in group II broth is placed with the help of micro pipette into root canal and at the same time apical 1/3 of tooth were immersed into broth with both groups for 8 weeks. Biomechanical preparation, obturation and coronal sealing done using GIC with group II, followed by apical 1/3 immersed in the enterococcus broth for 8 weeks, examination under confocal laser scanning microscope after splitting the teeth samples into two halfs buccolingually. Results: This study shows only adhesion of Enterococcus faecalis to root cementum. Conclusion Adhesion of Enterococcus faecalis to root cementum may be the possible reason for persisting infection after root canal treatment.

scholarly journals ENTEROCOCCUS FAECALIS CAUSE FOR PERSISTING INFECTION A CONFOCAL ANALYSIS

2013 ◽  
Vol 03 (04) ◽  
pp. 057-062
Author(s):  
Rahul Halkai ◽  
Mithra N. Hegde ◽  
Kiran Halkai
Keyword(s):  
Enterococcus Faecalis ◽  
Root Canal ◽  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Extreme Conditions ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Root Dentin

Abstract Aim: to know ability of Enterococcus faecalis invasion into root dentin. Methodology: Forty single rooted human intact teeth were selected, after access opening and canal debridement, all the samples were subjected for gamma sterilization to ensure complete absence of microorganisms, then exposed to Enterococcus faecalis broth, broth is placed with the help of micro pipette into root canal and also at the same time apical 1/3 of tooth were immersed into broth for 8 weeks, biomechanical preparation, obturation and coronal sealing done using GIC followed by examination under confocal laser scanning microscope after splitting the teeth samples into two halfs buccolingually. Results: This study shows invasion of Enterococcus faecalis upto 160 μm deep in to root dentin. Conclusion: penetration and survival of Enterococcus faecalis deep into dentin in extreme conditions may be the possible reason for persisting infection after root canal treatment.

3-D imaging of cells using a confocal laser scanning microscope and digital image processing

1988 ◽  
Vol 46 ◽  
pp. 96-97
Author(s):  
Thomas M. Jovin ◽  
Michel Robert-Nicoud ◽  
Donna J. Arndt-Jovin ◽  
Thorsten Schormann
Keyword(s):  
Laser Scanning ◽  
Confocal Laser Scanning Microscope ◽  
Laser Scanning Microscopy ◽  
Confocal Laser Scanning ◽  
Confocal Laser ◽  
Scanning Microscope ◽  
Laser Scanning Microscope ◽  
Biochemical Processes ◽  
Adjacent Structures

Light microscopic techniques for visualizing biomolecules and biochemical processes in situ have become indispensable in studies concerning the structural organization of supramolecular assemblies in cells and of processes during the cell cycle, transformation, differentiation, and development. Confocal laser scanning microscopy offers a number of advantages for the in situ localization and quantitation of fluorescence labeled targets and probes: (i) rejection of interfering signals emanating from out-of-focus and adjacent structures, allowing the “optical sectioning” of the specimen and 3-D reconstruction without time consuming deconvolution; (ii) increased spatial resolution; (iii) electronic control of contrast and magnification; (iv) simultanous imaging of the specimen by optical phenomena based on incident, scattered, emitted, and transmitted light; and (v) simultanous use of different fluorescent probes and types of detectors.We currently use a confocal laser scanning microscope CLSM (Zeiss, Oberkochen) equipped with 3-laser excitation (u.v - visible) and confocal optics in the fluorescence mode, as well as a computer-controlled X-Y-Z scanning stage with 0.1 μ resolution.

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