Isolation and identification of microbiota from Egyptian common insect pests invading economically important crops

2021 ◽  
pp. 5825-5830
Author(s):  
Waill A. Elkhateeb ◽  
Marwa O. ELnahas ◽  
Kareem M. Mousa ◽  
Adel A. Ahmed ◽  
Abdu Galib ALKolaibe ◽  
...  
Keyword(s):  
Insect Pests ◽  
Agrotis Ipsilon ◽  
Cotton Leaf ◽  
Paecilomyces Variotii ◽  
Isolation And Identification ◽  
Black Cutworm ◽  
Absidia Corymbifera ◽  
Control Programs ◽  
Fungal Microbiota ◽  
Bacteria And Fungi

Insects bodies contain multiple distinctive microbial colonies which play key role in the metabolism, development and health of an insect. In the current study bacterial and fungal microbiota were isolated from larval feces and adult wings of two major insect pests in Egypt, the Cotton leaf worm Spodoptera littoralis and the black cutworm Agrotis ipsilon. Isolated pathogens were identified and characterized according to the scientific described methods in such cases. Two different genera of bacteria (Bacillus & Serratia) were isolated from larval feces of both insects while isolated fungus were Paecilomyces variotii and Absidia corymbifera from the feces of A. ipsilon larvae, while Rhozopus stolonifer and Penicillium chrysogenum were isolated from adults wings of the same insect. The fungus Aspergillus flavus, Aspergillus niger and Mucor circinilloides were isolated from feces of S. littoralis larvae. Numerous species of bacteria and fungi had been documented as a bio-agent against many insect pests, and most of them exhibited disruption in life-cycle of different species of Lepidopterous pests. The obtained data sheds light on microbial colonies associated with two major insect pests, however further studies are required to involve the isolated microbiota in the biological control programs of insect pests.

scholarly journals Identification and Management of Pests and Diseases of Garden Crops in Santa, Cameroon

2019 ◽  
pp. 1-9
Author(s):  
Konje C. N. ◽  
Abdulai A. N. ◽  
Achiri Denis Tange ◽  
Nsobinenyui D. ◽  
Tarla D. N. ◽  
...  
Keyword(s):  
Insect Pests ◽  
Plasmodiophora Brassicae ◽  
Economic Losses ◽  
Agrotis Ipsilon ◽  
Wet Season ◽  
Black Cutworm ◽  
North West ◽  
Pests And Diseases ◽  
The North ◽  
West Region

Aims: This work sought to identify the pests and diseases that hinder successful gardening and the pesticides used to manage these pests and their frequencies of application. Place and Duration of Study: This work was carried out in the Santa Area of the North West Region of Cameroon. It was conducted from June 2013 to February 2014. Methodology: The study was carried out on six farms in Santa, a Sub-division in Mezam Division of the North West Region of Cameroon. On each of these farms, an area of 20 x 20 m was mapped and the plants therein observed. Insect pests, diseases and the pesticides used for their mitigation were identified at each growth stage. Results: The main diseases identified were clubroot (Plasmodiophora brassicae) and late blight (Phythophthora infestans) while Aphids (Myzus persicae S.), whiteflies (Bemisia tabaci) fruit worms (Helicoverpa amigera), Cutworms, fruitfly (Dacus punctatifrons) and grasshoppers (Zonocerus variegatus) were the prominent insect pests. The most applied pesticides were Cypermethrine and Dimethoate against insects, and Mancozeb and Maneb against fungi. Conclusion: From this study the most prominent pest of cabbage was the black cutworm (Agrotis ipsilon), which affected the early growing stage. The main disease that affected tomato was blight, seen in both seasons but its severity was greater in the rainy season.  Insect pests were a major problem in the dry season causing high economic losses than in the wet season.

scholarly journals Nano-Insecticides Against the Black Cutworm Agrotis ipsilon (Lepidoptera: Noctuidae): Toxicity, Development, Enzyme activity, and DNA Mutagenicity

2021 ◽  
Author(s):  
Mona A. M. Awad ◽  
El-Desoky S. Ibrahim ◽  
Engy I. Osman ◽  
Wael H. Elmenofy ◽  
Abdel Wahab M. Mahmoud ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Enzyme Activity ◽  
Insect Pests ◽  
Reproductive Activity ◽  
Developmental Time ◽  
Agrotis Ipsilon ◽  
Black Cutworm ◽  
Modern Agriculture ◽  
Mutagenic Effects ◽  
Chemical Pesticides

High-frequency doses of chemical pesticides cause environmental pollution with high pesticide residues. In addition, increasing insecticide resistance in many insect pests requires novel pest control methods. Nanotechnology could be a promising field of modern agriculture, and is receiving considerable attention in the development of novel nano-agrochemicals, such as nanoinsectticides and nanofertilizers. This study assessed the effects of the lethal and sublethal concentrations of chlorantraniliprole, thiocyclam, and their nano-forms on the development, reproductive activity, oxidative stress enzyme activity, and DNA changes at the molecular level of the polyphagous species of black cutworm Agrotis ipsilon. The results revealed that A. ipsilon larvae were more susceptible to the nano-formsthan the regular forms of both nano chlorine and sulfur within the chlorantraniliprole and thiocyclam insecticides, respectively, with higher toxicities than the regular forms (ca. 3.86, and ca.2.06-fold, respectively). Significant differences in biological parameters, including developmental time and reproductive activity (fecundity and hatchability percent) were also observed. Correspondingly, increases in oxidative stress enzyme activities were observed, as were mutagenic effects on the genomic DNA of A. ipsilon after application of the LC50 of the nano-forms of both insecticides compared to the control. The positive results obtained here have led us to apply these nano-forms indifferent insect models in additional studies.

scholarly journals Efficacy of the entomopathogenic nematode isolate Heterorhabditis taysearae to control the cotton leafworm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae)

2022 ◽  
Vol 32 (1) ◽  
Author(s):  
Amany M. Abd El Azim
Keyword(s):  
Spodoptera Littoralis ◽  
Insect Pests ◽  
Biological Control Agent ◽  
Entomopathogenic Nematode ◽  
Control Agent ◽  
Local Alignment ◽  
Cotton Leaf ◽  
Internal Transcribed Spacer Region ◽  
Control Programs ◽  
Lepidoptera Noctuidae

Abstract Background Entomopathogenic nematodes (EPNs) are promising alternatives since they have many characteristics as a biological control agent against insect pests. Results Among indigenous EPN isolated, adapted to local environmental conditions by employing the Galleria baiting strategy, only one sample was positive for the presence of EPNs. The new isolate was identified at the species level using DNA sequencing of the internal transcribed spacer region and the Basic Local Alignment Search Tool search of GenBank showed that the isolate had a high similarity (99%) with that sequence available for Heterorhabditis taysearae. The pathogenicity of the EPN isolate was tested against the cotton leaf worm, Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae), using different concentrations (60, 90, 120 and 150 IJs/larvae). Data showed that 150 IJs/larvae caused 100% mortality rate, followed by 120 IJs/larvae (90%), while 60 IJs/larvae showed the lowest rate (60%) after 72 h. Conclusions The present study indicated that the native isolate of EPN could be recommended against S. littoralis as an efficient tool in its control programs.

scholarly journals The Novel Agrotis ipsilon Nora Virus Confers Deleterious Effects to the Fitness of Spodoptera frugiperda (Lepidoptera: Noctuidae)

2021 ◽  
Vol 12 ◽  
Author(s):  
Tong Li ◽  
Ruobing Guan ◽  
Yuqing Wu ◽  
Su Chen ◽  
Guohui Yuan ◽  
...  
Keyword(s):  
Spodoptera Frugiperda ◽  
Rna Virus ◽  
Open Reading Frames ◽  
Agrotis Ipsilon ◽  
The Novel ◽  
Black Cutworm ◽  
A Genome ◽  
Nora Virus ◽  
Novel Host ◽  
Lepidoptera Noctuidae

In the present study, we identified a novel, positive-sense single-stranded RNA virus in the Chinese black cutworm, Agrotis ipsilon. It has a genome length of 11,312 nucleotides, excluding the poly(A) tails, and contains five open reading frames. The ORF2 encodes the conserved domains of RNA helicase and RNA-dependent RNA polymerase, while ORF4 and 5 encode three viral proteins. Herein, the A. ipsilon virus was clustered with a Helicoverpa armigera Nora virus and was thus provisionally named “Agrotis ipsilon Nora virus” (AINV). AINV was successfully transmitted into a novel host, Spodoptera frugiperda, through injection, causing a stable infection. This found the possibility of horizontal AINV transmission among moths belonging to the same taxonomic family. Nonetheless, AINV infection was deleterious to S. frugiperda and mainly mediated by antiviral and amino acid metabolism-related pathways. Furthermore, the infection significantly increased the S. frugiperda larval period but significantly reduced its moth eclosion rate. It suggests that AINV is probably to be a parasitic virus of S. frugiperda.

Designing of a Recombinant Agip Bacmid Construct with Infectious Properties Against Black Cutworm Agrotis ipsilon Larvae

2017 ◽  
Vol 183 (1) ◽  
pp. 307-317 ◽  
Author(s):  
Naglaa A. Abdallah ◽  
Wael El-Menofy ◽  
Abdelhadi Abdallah Abdelhadi
Keyword(s):  
Agrotis Ipsilon ◽  
Black Cutworm
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