Clinical evolution of hepatitis delta virus genotype 3 infection in patients from the Western Amazon, Brazil

Background: Chronic infection with the Hepatitis Delta Virus (HDV) is often associated with severe liver decompensation and fulminant hepatitis, but in some cases, it can present a stable clinical presentation. Objectives: This study evaluated the clinical evolution of HDV-3 carriers from an endemic region of the western Brazilian Amazon. Methods: Cross-sectional study was carried out with Anti-HDAg reagent patients, seen at an outpatient clinic specialized in viral hepatitis located in Rondônia, Brazil. Findings: A total of 19 patients, 68.4% male and 31.6% female, aged between 23 and 65 years old, were evaluated; 84.2% were clinically classified as carriers of the decompensated disease and 15.8% as carriers of the inactive disease. The results of the clinical evaluation were related to viral load; 30.8% had detectable viral RNA, and even though it was not possible to establish an association between the stage of the disease and persistent viral replication (p> 0.05), persistent viral replication was predictive of early evolution for liver cirrhosis. Conclusion: The results demonstrate the possibility that viral load can be used as a noninvasive hepatic marker in the clinical management of Hepatitis Delta. Keywords: chronic; hepatitis D; hepatitis delta virus; liver diseases.

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Novel hepatitis D-like agents in vertebrates and invertebrates

10.1101/539924 ◽

2019 ◽

Cited By ~ 1

Author(s):

Wei-Shan Chang ◽

John H.-O. Pettersson ◽

Callum Le Lay ◽

Mang Shi ◽

Nathan Lo ◽

...

Keyword(s):

Hepatitis Delta Virus ◽

Evolutionary History ◽

Rna Virus ◽

Coiled Coil ◽

The Novel ◽

Leucine Zippers ◽

Hepatitis Delta ◽

Hepatitis D ◽

B Virus ◽

Delta Virus

Hepatitis delta virus (HDV) is the smallest known RNA virus and encodes a single protein. Until recently, HDV had only been identified in humans, where it is strongly associated with co-infection with hepatitis B virus (HBV). However, the recent discovery of HDV-like viruses in metagenomic samples from birds and snakes suggests that this virus has a far longer evolutionary history. Herein, using additional meta-transcriptomic data, we show that highly divergent HDV-like viruses are also present in fish, amphibians and invertebrates. Notably, the novel viruses identified here share HDV-like genomic features such as a small genome size of ~1.7kb in length, circular genomes, and self-complementary, unbranched rod-like structures. Coiled-coil domains, leucine zippers, conserved residues with essential biological functions and isoelectronic points similar to those in the human hepatitis delta virus antigens (HDAgs) were also identified in the putative non-human HDAgs. Notably, none of these novel HDV-like viruses were associated with hepadnavirus infection, supporting the idea that the HDV-HBV association may be specific to humans. Collectively, these data not only broaden our understanding of the diversity and host range of HDV in non-human species, but shed light on its origin and evolutionary history.

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Phylodynamic and Phylogeographic Analysis of Hepatitis Delta Virus Genotype 3 Isolated in South America

Viruses ◽

10.3390/v11110995 ◽

2019 ◽

Vol 11 (11) ◽

pp. 995 ◽

Cited By ~ 1

Author(s):

Felipe Souza Nogueira-Lima ◽

Luan Felipo Botelho-Souza ◽

Tárcio Peixoto Roca ◽

Alcione Oliveira dos Santos ◽

Suyane da Costa Oliveira ◽

...

Keyword(s):

South America ◽

Hepatitis Delta Virus ◽

Recent Common Ancestor ◽

Screening Methods ◽

Genotype 3 ◽

Virus Genotype ◽

Serological Screening ◽

Hepatitis Delta ◽

Most Recent Common Ancestor ◽

Delta Virus

The hepatitis delta virus (HDV) is a globally distributed agent, and its genetic variability allows for it to be organized into eight genotypes with different geographic distributions. In South America, genotype 3 (HDV-3) is frequently isolated and responsible for the most severe form of infection. The objective of this study was to evaluate the evolutionary and epidemiological dynamics of HDV-3 over the years and to describe its distribution throughout this continent in an evolutionary perspective. While using Bayesian analysis, with strains being deposited in the Nucleotide database, the most recent common ancestor was dated back to 1964 and phylogenetic analysis indicated that the dispersion may have started in Brazil, spreading to Venezuela and then to Colombia, respectively. Exponential growth in the effective number of infections was observed between the 1950s and 1970s, years after the first report of the presence of HDV on the continent, during the Labrea Black Fever outbreak, which showed that the virus continued to spread, increasing the number of cases decades after the first reports. Subsequently, the analysis showed a decrease in the epidemiological levels of HDV, which was probably due to the implantation of the vaccine against its helper virus, hepatitis B virus, and serological screening methods implemented in the blood banks.

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A divergent hepatitis D-like agent in birds

10.1101/423707 ◽

2018 ◽

Cited By ~ 3

Author(s):

Michelle Wille ◽

Hans J. Netter ◽

Margaret Littlejohn ◽

Lilly Yuen ◽

Mang Shi ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Hepatitis Delta Virus ◽

Amino Acid Similarity ◽

Hepatitis Delta ◽

Hepatitis D ◽

Delta Antigen ◽

B Virus ◽

Severe Liver Disease ◽

Delta Virus

AbstractHepatitis delta virus (HDV) is currently only found in humans, and is a satellite virus that depends on hepatitis B virus (HBV) envelope proteins for assembly, release and entry. Using meta-transcriptomics, we identified the genome of a novel HDV-like agent in ducks. Sequence analysis revealed secondary structures that were shared with HDV, including self-complementarity and ribozyme features. The predicted viral protein shares 32% amino acid similarity to the small delta antigen of HDV and comprises a divergent phylogenetic lineage. The discovery of an avian HDV-like agent has important implications for the understanding of the origins of HDV and subviral agents.ImportanceHepatitis delta virus (HDV) is currently only found in humans, and coinfections of HDV and Hepatitis B virus (HBV) in humans result in severe liver disease. There are a number of hypotheses for the origin of HDV, although a key component of all is that HDV only exists in humans. Here, we describe a novel deltavirus-like agent identified in wild birds. Although this agent is genetically divergent, it exhibits important similarities to HDV, such as the presence of ribosymes and self-complementarity. The discovery of an avian HDV-like agent challenges our understanding of both the origin and the co-evolutionary relationships of subviral agents with helper viruses.

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Initiation of RNA replication of cloned Taiwan-3 isolate of hepatitis delta virus genotype II in cultured cells

Biochemical and Biophysical Research Communications ◽

10.1016/s0006-291x(03)01076-3 ◽

2003 ◽

Vol 306 (4) ◽

pp. 966-972 ◽

Cited By ~ 14

Author(s):

Feng-Ming Lin ◽

Chuan-Mo Lee ◽

Tzu-Chi Wang ◽

Mei Chao

Keyword(s):

Hepatitis Delta Virus ◽

Cultured Cells ◽

Rna Replication ◽

Virus Genotype ◽

Hepatitis Delta ◽

Genotype Ii ◽

Delta Virus

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The Prevalence of Hepatitis D Virus in Reactive HBsAg Blood Donors at Department of Pathology, LUMHS Jamshoro

10.53350/pjmhs2115103126 ◽

2021 ◽

Vol 15 (10) ◽

pp. 3126-3129

Author(s):

Sadia Akbar ◽

Nazia Mumtaz ◽

Sana Fatema ◽

Ikram Din Ujjan ◽

Kiran Aamir ◽

...

Keyword(s):

Hepatitis B ◽

Hepatitis Delta Virus ◽

Research Laboratory ◽

Blood Donors ◽

Local Level ◽

Hepatitis Delta ◽

Hepatitis D ◽

Civil Hospital ◽

Hepatitis D Virus ◽

Delta Virus

Background: The hepatitis delta virus (HDV) is a defective hepatotropic virus that only affects patients infected with the hepatitis B virus (HBV). Infection with the hepatitis delta virus can cause acute hepatitis, including the fulminant presentation or spontaneously resolving infection and chronic infection Aim: The present study's aim was to determine the prevalence of the Hepatitis D virus in reactive HBsAg blood donors at Diagnostic and Research Lab Hyderabad. Materials and Methods: This cross-sectional study was conducted on 434 blood donors at the Pathology department (Diagnostic and Research Laboratory) Civil Hospital, Hyderabad from January 2017 to December 2017. All the healthy individuals who visited at Diagnostic and Research Laboratory of Civil Hospital, LUMHS Hyderabad as blood donors with specific age groups of either gender were included in this study. All the individuals underwent Hepatitis screening. All of those cases that were noted with positive HBV further underwent HDV screening test. All the data was recorded in the proforma for the purposed of analysis. SPSS version 20 was used for data analysis. Results: Of the total 434 blood donors, 420 (96.8%) were male and 14 (3.2%) were female. The overall mean age was 31.65±4.67 years with an age range of 18 to 60 years. Out of 434, the incidence of positive delta virus was 62 (14.3%) while the remaining 372 (85.7%) were negative delta hepatitis blood donors. The prevalence of delta hepatitis blood donors with respect to age distribution were as follows; 18-30 years had 208 (48%), 31-40 years had 188 (27%), 41-50 years 69 (16%), and 51-60 years 39 (9%). Of the total 62 positive delta hepatitis, the prevalence of males and females was 98.4% and 1.6% respectively. All the donor’s blood group was divided into +O, +B, +A, +AB, -O, -A, and –B with their respective prevalence was 48.6%, 22.6%. 11.5%, 8.8%, 2.5%, 3.5%, and 2.5% whereas their frequency in positive tested delta hepatitis was 36 (58.1%), 7 (11.3%), 10 (16.1%), 6 (9.7%), 1 (1.6%), 2 (3.2%), and 0 (0%) respectively. Conclusion: It is concluded that the prevalence of HDV is 14.3% in Hepatitis B reactive healthy donors. This is a higher prevalence as compared to previously published studies. No such adequate recent data is available at the local level. More research is needed on this event, to provide adequate knowledge, which will be helpful to clinical and laboratory investigators, and physicians to reduce the burden of liver disease caused by HBV and HDV co-infection. Keywords: Hepatitis delta virus; HBsAg reactivity; Blood donors;

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Genotype Analysis, Using PCR with Type-Specific Primers, of Hepatitis B Virus Isolates from Patients Coinfected with Hepatitis Delta Virus Genotype II from Miyako Island, Japan

Intervirology ◽

10.1159/000069746 ◽

2003 ◽

Vol 46 (2) ◽

pp. 114-120 ◽

Cited By ~ 3

Author(s):

Mitsuhiko Moriyama ◽

Masaaki Taira ◽

Hiroshi Matsumura ◽

Hiroshi Aoki ◽

Morio Mikuni ◽

...

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Hepatitis Delta Virus ◽

Virus Genotype ◽

Specific Primers ◽

Hepatitis Delta ◽

B Virus ◽

Virus Isolates ◽

Genotype Ii ◽

Delta Virus

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New Enzyme-Linked Immunosorbent Assay for Detection of Antibodies against Hepatitis Delta Virus Using a Hepatitis Delta Antigen Derived from a Taiwanese Clone and Comparison to the Abbott Radioimmunoassay

Clinical and Vaccine Immunology ◽

10.1128/cvi.05687-11 ◽

2012 ◽

Vol 19 (5) ◽

pp. 817-819 ◽

Cited By ~ 5

Author(s):

Yung-Bin Kuo ◽

Mei Chao ◽

Yi-Hsuan Lee ◽

Chau-Ting Yeh ◽

Err-Cheng Chan

Keyword(s):

Immunosorbent Assay ◽

Hepatitis Delta Virus ◽

Detection Efficiency ◽

Enzyme Linked Immunosorbent Assay ◽

Hepatitis Delta ◽

Hepatitis D ◽

Delta Antigen ◽

Hepatitis Delta Antigen ◽

Hdv Infection ◽

Delta Virus

ABSTRACTAn anti-hepatitis delta (HD) enzyme-linked immunosorbent assay (ELISA) using a specific recombinant hepatitis delta antigen derived from a local dominant hepatitis delta virus (hepatitis D virus; HDV) strain in Taiwan has been established. The detection efficiency of this assay was comparable to that of the commercially available Abbott anti-HD radioimmunoassay (RIA) and could be useful in routine laboratory diagnoses of HDV infection.

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