scholarly journals Regeneration and Transformation of Gene Encoding the Hemagglutinin Antigen of the H5N1 Virus in Frond of Duckweed (Spirodela polyrhiza L.)

2015 ◽  
Vol 3 (1) ◽  
pp. 48 ◽  
Author(s):  
Pham Thi Ly Thu ◽  
Pham Thi Huong ◽  
Vu Van Tien ◽  
Le Huy Ham ◽  
Tran Dang Khanh
Keyword(s):  
H5n1 Virus ◽  
Low Cost ◽  
Human Health Risk ◽  
Economic Loss ◽  
Pcr Analysis ◽  
Mineral Concentration ◽  
Gene Encoding ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza ◽  
Ha1 Gene

H5N1 (Avian influenza virus) has caused economic loss and remained a serious human health risk in many countries, including Vietnam. Plant-derived vaccines have offered the advantages over tradition subunit vaccine with low-cost production potential. Duckweed (Sprirodela polyrhiza L.) has found a valuable target plant for various application. In this study, we have attempted to re-generate and transform the gene encoding the hemagglutinin antigen of H5N1 virus in frond of S. polyrhiza, a widely great duckweed grown in Vietnam. The results have shown that the culture medium for S. polyrhiza duckweed is Hutner medium with reduced mineral concentration ½ (H/2) supplemented with 10g/l sucrose at pH 6. This medium showed appropriate for the best growth and development of duckweed. Results of PCR analysis of S. polyrhiza lines from 14 transformation experiments that has obtained 4 duckweed lines carrying HA1 gene. This prelinary results will be provide the basis for transgenic research in the dukweed species and be useful for further generate the producing enable vaccine via the transgenic plants

Peptide mimics of a conserved H5N1 avian influenza virus neutralization site

2009 ◽  
Vol 419 (1) ◽  
pp. 133-139 ◽  
Author(s):  
Wenxin Luo ◽  
Yingwei Chen ◽  
Mingqiao Wang ◽  
Yixin Chen ◽  
Zhenhua Zheng ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Fusion Proteins ◽  
H5n1 Virus ◽  
Amino Acid Sequences ◽  
Antigenic Determinants ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Influenza ◽  
H5n1 Avian Influenza

A panel of 52 murine monoclonal antibodies was found to recognize antigenic determinants that had been conserved among all major genetic subgroups of the H5N1 avian influenza virus prevalent since 1997. We screened a phage display library for peptides recognized by one such antibody (8H5). We analysed the specificity of 8H5 for reactive peptides presented as fusion proteins of HBc (hepatitis B core protein) and HEV (hepatitis E virus) structural protein, p239. This was then related to the specificity of the native HA (haemagglutinin) molecule by virtue of the capacity of fusion proteins to compete for 8H5 binding with different strains of H5N1 virus and the reactivity of antisera generated against fusion proteins to bind native HA molecules, and to inhibit haemagglutination and arrest infection by the virus. Nine reactive peptides of different amino acid sequences were identified, six of which were also reactive with the antibody in association with HBc and four were in association with p239. Binding occurred with the dimeric form of the four p239-fusion proteins and one of the HBc-fusion proteins, but not with the monomeric form. The HBc-fusion proteins blocked 8H5 binding with four strains of H5N1 influenza virus. Mouse antisera generated against fusion proteins bound to HA molecules, but did not inhibit haemagglutination or arrest H5N1 infection. Our findings indicate that 8H5 recognizes discontinuous sites presented by secondary and possibly higher structural orders of the peptides in spatially favourable positions for binding with the antibody, and that the peptides partially mimic the native 8H5 epitopes on the H5N1 virus.

scholarly journals Origin of highly pathogenic H5N1 avian influenza virus in China and genetic characterization of donor and recipient viruses

2007 ◽  
Vol 88 (11) ◽  
pp. 3094-3099 ◽  
Author(s):  
Muhammad Mahmood Mukhtar ◽  
Sahibzada T. Rasool ◽  
Degui Song ◽  
Chengliang Zhu ◽  
Qian Hao ◽  
...  
Keyword(s):  
Avian Influenza ◽  
H5n1 Virus ◽  
Influenza Viruses ◽  
Jiangxi Province ◽  
Highly Pathogenic ◽  
Genetic Characteristics ◽  
H5n1 Avian Influenza Virus ◽  
Ha Gene ◽  
H5n1 Avian Influenza ◽  
Hpai H5n1

Genetic analysis of all eight genes of two Nanchang avian influenza viruses, A/Duck/Nanchang/1681/92 (H3N8-1681) and A/Duck/Nanchang/1904/92 (H7N1-1904), isolated from Jiangxi province, China, in 1992, showed that six internal genes of H3N8-1681 virus and five internal (except NS gene) genes of H7N1-1904 virus were closely similar to A/Goose/Guangdong/1/96 (H5N1) virus, the first highly pathogenic avian influenza (HPAI) virus of subtype H5N1 isolated in Asia. The neuraminidase (NA) gene of Gs/Gd/1/96 had the highest genetic similarity with A/Duck/Hokkaido/55/96 (H1N1-55) virus. The haemagglutinin (HA) gene of Gs/Gd/1/96 virus might have originated as a result of mutation of H5 HA gene from A/Swan/Hokkaido/51/96 (H5N3-51)-like viruses. The PA gene of H5N3-51 virus had the highest similarity with Gs/Gd/1/96. This study explains the origin of first Asian HPAI H5N1 virus in Guangdong by the reassortment of Nanchang (close to Guangdong) and Hokkaido (Japan) (H1N1-55 and H5N3-51) viruses. Genetic characteristics of donor and recipient viruses were also studied.

scholarly journals H5N1 avian influenza virus: human cases reported in southern China

2003 ◽  
Vol 7 (9) ◽  
Author(s):  
J Crofts ◽  
W J Paget ◽  
F Karcher
Keyword(s):  
Hong Kong ◽  
Avian Influenza ◽  
Influenza A ◽  
H5n1 Virus ◽  
Southern China ◽  
Influenza Infection ◽  
Respiratory Illness ◽  
Respiratory Illnesses ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza

Two cases of confirmed influenza due to the avian influenza A H5N1 virus were reported last week in Hong Kong (1). The cases occurred in a Hong Kong family who had recently visited Fujian province in southern China. The daughter, aged 8 years, died following a respiratory illness. The cause of her death is unknown. The father and son also had respiratory illnesses; the father died and the son recovered. Both were infected with the H5N1 virus. The mother also had a respiratory illness, which is reported to have been unrelated to influenza infection. Investigations are continuing to determine where and how transmission of infection to the cases may have occurred.

scholarly journals Near-Complete Genome Sequence of an H5N1 Avian Influenza Virus Strain Isolated from a Swan in Southwest Kazakhstan in 2006

2020 ◽  
Vol 9 (13) ◽  
Author(s):  
Yerbol Burashev ◽  
Vitaliy Strochkov ◽  
Kulyaisan Sultankulova ◽  
Mukhit Orynbayev ◽  
Markhabat Kassenov ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genome Sequence ◽  
Virus Strain ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
H5n1 Virus ◽  
Surface Protein ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza ◽  
Influenza H5n1

We report the near-complete genome sequence of an influenza H5N1 virus strain isolated from a dead swan on the southeastern Caspian seashore in 2006. The results of the surface protein HA phylogenetic analysis showed that the A/swan/Mangystau/3/2006 virus belongs to the EA-nonGsGD clade.

BioBoard

2006 ◽  
Vol 10 (22) ◽  
pp. 1267-1273
Keyword(s):  
Hong Kong ◽  
New Zealand ◽  
South Korea ◽  
Drug Approval ◽  
Influenza Virus Vaccine ◽  
Biomedical Ethics ◽  
Bird Flu ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza ◽  
Speed Up

Australia — Politicians Chastise Australia's Science Institute. Australia — GE Healthcare and WA Government Collaborate on Cell-based Imaging Equipment. Australia — The Goal of Imugene's H5N1 Avian Influenza Virus Vaccine. China — East China University Sparks Debate on Education Funding. China — 3D Map of SARS virus Drawn. China — Researchers Comment that Global Loss of Biodiversity is Harming Ocean Bounty. China — China Insists that there are No Variant Bird Flu Strain. China — Gene involved in Eye Lens Development. China — Cancer-causing Dye Found in Duck Eggs in China. Hong Kong — Scientists in Hong Kong Found Clues to Pandemic Bird Flu. Hong Kong — Hong Kong Bird Flu Expert Picked to Head WHO. India — Ranbaxy Signs Licensing Agreement with Swiss Company Debiopharm. India — Indian Biotechnology Park. Japan — Japan's New Premier Chases Innovation. Japan — Japan Reforms Screening to Speed up Drug Approval. New Zealand — New Zealand Invests in Neurology Project. South Korea — South Korea Gives Funding Boost to Stem-Cell Research. South Korea — South Korea Plans to Inject $253 million into Biotech. South Korea — Scientists Discover Stem Cells Might Help to Treat Mental Illness. Singapore — Renowned French Cancer Development Biologist Moves to Singapore's Biopolis. Singapore — Singapore Plans to Build Bigger Heart Center to Handle Spiraling Patient Numbers. Singapore — New Centre for Biomedical Ethics at NUS. Taiwan — Taiwan's CDC Places Order for H5N1 Vaccine. Taiwan — Tenders sought for Pingtung Agricultural Biotech Park Housing.

scholarly journals Evaluation of a rapid test for detection of H5N1 avian influenza virus

2008 ◽  
Vol 154 (1-2) ◽  
pp. 213-215 ◽  
Author(s):  
Yixin Chen ◽  
Feihai Xu ◽  
Xiaohui Fan ◽  
Haifeng Luo ◽  
Shengxiang Ge ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Rapid Test ◽  
H5n1 Avian Influenza Virus ◽  
H5n1 Avian Influenza

scholarly journals Pathobiology of A/Chicken/Hong Kong/220/97 (H5N1) Avian Influenza Virus in Seven Gallinaceous Species

2001 ◽  
Vol 38 (2) ◽  
pp. 149-164 ◽  
Author(s):  
L. E. L. Perkins ◽  
D. E. Swayne
Keyword(s):  
Hong Kong ◽  
Avian Influenza ◽  
Pulmonary Edema ◽  
Multiorgan Failure ◽  
Clinical Manifestations ◽  
Highly Pathogenic ◽  
H5n1 Avian Influenza Virus ◽  
Multiple Organs ◽  
H5n1 Avian Influenza ◽  
Virus Localization

Direct bird-to-human transmission, with the production of severe respiratory disease and human mortality, is unique to the Hong Kong-origin H5N1 highly pathogenic avian influenza (HPAI) virus, which was originally isolated from a disease outbreak in chickens. The pathobiology of the A/chicken/Hong Kong/ 220/97 (H5N1) (HK/220) HPAI virus was investigated in chickens, turkeys, Japanese and Bobwhite quail, guinea fowl, pheasants, and partridges, where it produced 75-100% mortality within 10 days. Depression, mucoid diarrhea, and neurologic dysfunction were common clinical manifestations of disease. Grossly, the most severe and consistent lesions included splenomegaly, pulmonary edema and congestion, and hemorrhages in enteric lymphoid areas, on serosal surfaces, and in skeletal muscle. Histologic lesions were observed in multiple organs and were characterized by exudation, hemorrhage, necrosis, inflammation, or a combination of these features. The lung, heart, brain, spleen, and adrenal glands were the most consistently affected, and viral antigen was most often detected by immunohistochemistry in the parenchyma of these organs. The pathogenesis of infection with the HK/220 HPAI virus in these species was twofold. Early mortality occurring at 1-2 days postinoculation (DPI) corresponded to severe pulmonary edema and congestion and virus localization within the vascular endothelium. Mortality occurring after 2 DPI was related to systemic biochemical imbalance, multiorgan failure, or a combination of these factors. The pathobiologic features were analogous to those experimentally induced with other HPAI viruses in domestic poultry.

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