scholarly journals A Network Pharmacology Approach to Determining the Mechanism of Eucommia ulmoides–Epimedium brevicornu Maxim against Osteoporosis

2021 ◽  
Vol 1 (3) ◽  
pp. 18
Author(s):  
Hefang Xiao ◽  
Meng Zhang ◽  
Jia Zheng
Keyword(s):  
Network Pharmacology ◽  
Eucommia Ulmoides

scholarly journals Effect of Eucommia ulmoides leaves on hyperuricemia and kidney injury induced by a high-fat/high-fructose diet in rats

2021 ◽  
Author(s):  
Man Gong ◽  
Hong Zhang ◽  
Xiaoqian Liu ◽  
Qingxia Li ◽  
Yang Zhang ◽  
...  
Keyword(s):  
Uric Acid ◽  
Molecular Docking ◽  
Renal Injury ◽  
Endocrine Resistance ◽  
Network Pharmacology ◽  
Eucommia Ulmoides ◽  
High Fat ◽  
Protein Database ◽  
High Fructose Diet ◽  
High Fructose

Abstract Eucommia ulmoides leaves have unique advantages in the treatment of metabolic diseases. In this study, network pharmacology and molecular-docking methods were used to predict the effects and action mechanisms of the major components of E. ulmoides leaves on hyperuricemia. Combining literature collection, we used SciFinder and the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform to collect E. ulmoides leaf flavonoid and iridoid components. Swiss Target Prediction, SEA, GeneCards, and the OMIM database were used to obtain core targets, and the STRING protein database was performed as core targets for gene ontology enrichment Set and KEGG analyses. Molecular docking was applied to predict the pathways regulating the metabolism of uric acid. The selected targets and targeting efficacy were validated using a rat model of hyperuricemia and renal injury induced by a high-fat and high-fructose diet. A total of 32 chemical components with effective targets, which regulated the PI3K-AKT pathway and endocrine resistance, were collected. Isoquercetin, kaempferol, and quercetin were predicted via network pharmacology to have potential bioactivities and strong docking binding forces. Molecular docking results showed that iridoids and flavonoids are bound to proteins related to inflammation and uric acid metabolism. In addition, it was verified via animal experiments that an E. ulmoides leaf extract ameliorated hyperuricemia, renal injury, and inflammation, which are closely related to the targets IL-6, TNF-α, TLR4, and GLUT9. In conclusion, E. ulmoides leaf flavonoids and iridoids ameliorate hyperuricemia and uric-acid–induced inflammation through a multi-component, multi-target, and multi-pathway mechanism, which provides a theoretical basis for the development of therapeutics from E. ulmoides leaf components.

scholarly journals Exploring the potential therapeutic effect of Eucommia ulmoides–Dipsaci Radix herbal pair on osteoporosis based on network pharmacology and molecular docking technology

RSC Advances ◽  
2022 ◽  
Vol 12 (4) ◽  
pp. 2181-2195
Author(s):  
Shuai Feng ◽  
Ting Wang ◽  
Liming Fan ◽  
Xinxin An ◽  
Xinli Ding ◽  
...  
Keyword(s):  
Molecular Docking ◽  
Therapeutic Effect ◽  
Interaction Mechanism ◽  
Network Pharmacology ◽  
Eucommia Ulmoides ◽  
Treatment Of Osteoporosis ◽  
Pathway Interaction

This study elaborated the multi-component, multi-target, and multi-pathway interaction mechanism of Eucommia ulmoides-Dipsaci Radix herbal pair in the treatment of osteoporosis.

Anti-osteoporotic effect of Eucommia ulmoides cortex in ovariectomized rats

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
B Kim ◽  
DW Lim ◽  
J Song ◽  
H Kim
Keyword(s):  
Ovariectomized Rats ◽  
Eucommia Ulmoides

Introduction of Eucommia ulmoides Oliv. to in vitro Culture

2020 ◽  
pp. 38-50
Author(s):  
A.V. Zhigunov ◽  
◽  
Q.T. Nguyen
Keyword(s):  
Herbal Medicines ◽  
Naphthaleneacetic Acid ◽  
Eucommia Ulmoides ◽  
Juvenile Period ◽  
Dioecious Species ◽  
Relict Species ◽  
Mass Reproduction ◽  
Special Value ◽  
The Republic

The increasing need for herbal medicines requires the study of not only biological resources of medical plants, but also methods for their reproduction. Of special value are the medicinal plants that have a long history of success in traditional medicine. One of such plants is Eucommia ulmoides Oliv., which belongs to a rare relict species growing in natural conditions, for the most part, in the undergrowth of humid subtropical forests in China, mainly in the middle course of the Yangtze river. E. ulmoides compares favorably with most subtropical plants owing to its significant frost resistance, which makes it possible to cultivate it outside the humid subtropics. It has been widely introduced in Krasnodar Krai and in the Republic of Adygea (Russia) since the mid-20th century and successfully adapted to various environmental conditions in the Northwest Caucasus. The increasing demand for E. ulmoides bark can only be satisfied by laying out industrial plantations. However, the difficulties encountered in the traditional seed reproduction of E. ulmoides (dioecious species, pollen low quality, parthenocarpy, prolonged seed dormancy, irregular fruiting, long juvenile period, etc.) make scientists turn to modern biotechnological methods of plant propagation. While considering cultivation of planting material, we should focus on highly efficient methods that ensure stable and mass reproduction of the plants under study. An important role is played here by in vitro plant regeneration. The effectiveness of biotechnology methods is due to a reduction in timing of obtaining a large number of vegetative progeny of plants difficult for propagation, as well saving of the area required for their cultivation. The conditions for producing an aseptic culture of E. ulmoides were chosen based on the results of the studies. The highest degree of sterilization of E. ulmoides shoot segments was achieved when the explants were sequentially immersed first in 70 % ethanol (30 s) and then in 0.1 % mercuric chloride solution (5 min). With such a sterilization procedure, 63.3 % of the studied cuttings were made sterile, and 56.7 % of them proved to be viable. The optimal composition of the nutrient medium for regeneration of E. ulmoides microshoots has been determined: MS medium complemented with 1 mg/L 6-Benzylaminopurine (BAP) + 0.2 mg/L 1-Naphthaleneacetic acid (NAA). The best media for explant rooting are the following: 2/3 MS + 1.5 mg/L NAA + 30 g sucrose + 7 g agar; 2/3 MS + 1 mg/L NAA + 0.4 mg/L IBA + 30 g sucrose + 7 g agar.

Exploring the Mechanism of Lingzhu San in Treating Febrile Seizures by Using Network Pharmacology

2020 ◽  
Vol 23 ◽  
Author(s):  
Xiao Zhou ◽  
Xiao-Fei Zhang ◽  
Dong-Yan Guo ◽  
Yan-Jun Yang ◽  
Lin Liu ◽  
...  
Keyword(s):  
Febrile Seizures ◽  
Network Pharmacology ◽  
Biological Processes ◽  
Active Compounds ◽  
Potential Core ◽  
R Language ◽  
The Core ◽  
Multiple Factors ◽  
Therapeutic Mechanism

Objective: Lingzhu San (LZS) is a traditional Chinese medicine (TCM) prescription which can be effective in treating febrile seizures (FS) and has few researches on the mechanisms. In order to better guide the clinical use of LZS, we used the research ideas and methods of network pharmacology to find the potential core compounds, targets and pathways of LZS in the complex TCM system for the treatment of FS, and predict the mechanism. Materials and Methods: Databases such as BATMAN, TCMSP, TCMID, and SWISS TARGET are used to mine the active compounds and targets of LZS, and the target information of FS was obtained through GENECARDS and OMIM. Using Venny2.1.0 and Cytoscape software to locked the potential core compounds and targets of FS. The R language and ClusterProfiler software package were adopt to enrich and analyze the KEGG and GO pathways of the core targets and the biological processes and potential mechanisms of the core targets were revealed. Results: 187 active compounds and 2113 target proteins of LZS were collected. And 38 potential core compounds, 35 core targets and 775 metabolic and functional pathways were screened which involved in mediating FS. Finally, the role of the core compounds, targets and pivotal pathways of LZS regulated FS in the pathogenesis and therapeutic mechanism of FS was discussed and clarified. Conclusions: In this paper, the multi-compounds, multi-targets and multi-pathways mechanism of LZS in the treatment of FS was preliminarily revealed through the analysis of network pharmacology data, which is consistent with the principle of multi-compounds compatibility of TCM prescriptions and unified treatment of diseases from multiple angles, and it provides a new way for TCM to treat complex diseases caused by multiple factors.

Network Pharmacology of Ayurveda Formulation Triphala with Special Reference to Anti-Cancer Property

2015 ◽  
Vol 18 (9) ◽  
pp. 846-854 ◽  
Author(s):  
Uma Chandran ◽  
Neelay Mehendale ◽  
Girish Tillu ◽  
Bhushan Patwardhan
Keyword(s):  
Special Reference ◽  
Network Pharmacology ◽  
Anti Cancer
Sign in / Sign up

Export Citation Format

Share Document