potential core
Recently Published Documents


TOTAL DOCUMENTS

321
(FIVE YEARS 118)

H-INDEX

33
(FIVE YEARS 4)

F1000Research ◽  
2022 ◽  
Vol 11 ◽  
pp. 38
Author(s):  
Mikhail Raevskiy ◽  
Anna Kondrashina ◽  
Yulia Medvedeva

Identification of transcription factors (TFs) that could induce and direct cell conversion remains a challenge. Though several hundreds of TFs are usually transcribed in each cell type, the identity of a cell is controlled and can be achieved through the ectopic overexpression of only a small subset of so-called core TFs. Currently, the experimental identification of the core TFs for a broad spectrum of cell types remains challenging. Computational solutions to this problem would provide a better understanding of the mechanisms controlling cell identity during natural embryonic or malignant development, as well as give a foundation for cell-based therapy. Herein, we propose a computational approach based on over-enrichment of transcription factors binding sites (TFBS) in differentially accessible chromatin regions that could identify the potential core TFs for a variety of primary human cells involved in hematopoiesis. Our approach enables the integration of both transcriptomic (single-cell RNA sequencing, scRNA-seq) and epigenenomic (single-cell assay for transposable-accessible chromatin, scATAC-seq) data at the single-cell resolution to search for core TFs, and can be scalable to predict subsets of core TFs and their role in a given conversion between cells.


Author(s):  
Olanrewaju Miracle Oyewola ◽  
Olawale Saheed Ismail ◽  
Lateef Anjola Sanni

This paper studies the effect of Reynolds number on a two-dimensional free incompressible isothermal coaxial turbulent jet over a range of high Reynolds numbers. This is necessary because of its application in noise control and mixing. The Reynolds numbers at the nozzle exit were 9824, 19648, 29472, 39296 and 49120. The models were designed in ANSYS Design Modeler and the numerical simulation was done using a finite volume based Computational Fluid Dynamics (CFD) in ANSYS FLUENT using the two-dimensional Realizable turbulence model. The Governing equations were discretized using the finite volume method with the solution based on the PISO algorithm. The decay of centerline velocity, turbulent kinetic energy profile, the radial profile of axial velocity and similarity profile were investigated along the flow direction. Contour plot indicates that the velocity is high at the jet exit and decreases downstream due to the rapid mixing of the inner and outer jet and the surrounding fluid. It is found generally that Reynolds number plays significant role especially before self-similarity region. The result shows that increasing the Reynolds number give rise to more turbulence which in turn decreases the potential core length, turbulent kinetic energy and enhances the mixing of the fluid. However, at the jet exit, the flow with the lowest Reynolds number has the highest turbulent kinetic energy because it suffers the greater shear. The spreading of the jet was more or less independent of the Reynolds number beyond the self-similarity region. It is also found that the velocity profile is brought to congruence at about z/D=25 for the Reynolds numbers considered


BMC Cancer ◽  
2022 ◽  
Vol 22 (1) ◽  
Author(s):  
Guanran Zhang ◽  
Xuyue Liu ◽  
Zhengyang Sun ◽  
Xiaoning Feng ◽  
Haiyan Wang ◽  
...  

Abstract Background Intrahepatic cholangiocarcinoma (ICC) is a type of malignant tumor ranking the second in the incidence of primary liver cancer following hepatocellular carcinoma. Both the morbidity and mortality have been increasing in recent years. Small duct type of ICC has potential therapeutic targets. But overall, the prognosis of patients with ICC is usually very poor. Methods To search latent therapeutic targets for ICC, we programmatically selected the five most suitable microarray datasets. Then, we made an analysis of these microarray datasets (GSE26566, GSE31370, GSE32958, GSE45001 and GSE76311) collected from the Gene Expression Omnibus (GEO) database. The GEO2R tool was effective to find out differentially expressed genes (DEGs) between ICC and normal tissue. Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis were executed using the Database for Annotation, Visualization and Integrated Discovery (DAVID) v 6.8. The Search Tool for the Retrieval of Interacting Genes (STRING) database was used to analyze protein–protein interaction of these DEGs and protein–protein interaction of these DEGs was modified by Cytoscape3.8.2. Survival analysis was performed using Gene Expression Profiling Interactive Analysis (GEPIA) online analysis tool. Results A total of 28 upregulated DEGs and 118 downregulated DEGs were screened out. Then twenty hub genes were selected according to the connectivity degree. The survival analysis results showed that A2M was closely related to the pathogenesis and prognosis of ICC and was a potential therapeutic target for ICC. Conclusions According to our study, low A2M expression in ICC compared to normal bile duct tissue was an adverse prognostic factor in ICC patients. The value of A2M in the treatment of ICC needs to be further studied.


Author(s):  
Simon A. Carter ◽  
Liz Lightstone ◽  
Dan Cattran ◽  
Allison Tong ◽  
Arvind Bagga ◽  
...  

Background and objectivesOutcomes reported in trials in adults with glomerular disease are often selected with minimal patient input, are heterogeneous, and may not be relevant for clinical decision making. The Standardized Outcomes in Nephrology–Glomerular Disease (SONG-GD) initiative aimed to establish a core outcome set to help ensure that outcomes of critical importance to patients, care partners, and clinicians are consistently reported.Design, setting, participants, and measurementsWe convened two 1.5-hour workshops in Melbourne, Australia, and Washington, DC, United States. Attendees were identified purposively with 50 patients/care partners and 88 health professionals from 19 countries; 51% were female. Patients and care partners were from the United States, Australia, and Canada, and had experience of a glomerular disease with systemic features (n=9), kidney-limited nephrotic disease (n=9), or other kidney-limited glomerular disease (n=8). Attendees reviewed the results of the SONG-GD Delphi survey and aims of the workshop and then discussed potential core outcomes and their implementation in trials among moderated breakout groups of eight to 12 people from diverse backgrounds. Transcripts of discussions were analyzed thematically.ResultsThree themes were identified that supported the proposed core outcomes: limiting disease progression, stability and control, and ensuring universal relevance (i.e., applicable across diverse populations and settings). The fourth theme, preparedness for implementation, included engaging with funders and regulators, establishing reliable and validated measures, and leveraging existing endorsements for patient-reported outcomes.ConclusionsWorkshop themes demonstrated support for kidney function, disease activity, death, life participation, and cardiovascular disease, and these were established as the core outcomes for trials in adults with glomerular disease. Future work is needed to establish the core measures for each domain, with funders and regulators central to the uptake of the core outcome set in trials.


2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Unnikrishnan Payyappallimana

Beginning with a brief recent history of plural health systems in the Indian context, this is a commentary on the idea of resilience from the perspectives of AYUSH and local health traditions (LHTs) as witnessed historically and during the COVID pandemic. By narrating the AYUSH systems’ experiences during COVID-19, in providing health care and in attempts at building rigorous research and evidence, it examines their potential future engagement in the public health scenario in the country. The article contextualizes the potential core functions of plural and integrative health systems for the resilience of the Indian health system.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lipeng Niu ◽  
Ce Gao ◽  
Yang Li

AbstractColorectal carcinoma (CRC) is one of the most prevalent malignant tumors worldwide. Meanwhile, the majority of CRC related deaths results from liver metastasis. Gene expression profile of CRC patients with liver Metastasis was identified using 4 datasets. The data was analyzed using GEO2R tool. GO and KEGG pathway analysis were performed. PPI network of the DEGs between 1 and 2 gene sets was also constructed. The set 1 is named between primary CRC tissues and metastatic CRC tissues. The set 2 is named between primary CRC tissues and normal tissues. Finally, the prognostic value of hub genes was also analyzed. 35 DEGs (set 1) and 142 DEGs (set 2) were identified between CRC liver metastatic cancer patients. The PPI network was constructed using the top 10 set 1 hub genes which included AHSG, SERPINC1, FGA, F2, CP, ITIH2, APOA2, HPX, PLG, HRG and set 2 hub genes which included TIMP1, CXCL1, COL1A2, MMP1, AURKA, UBE2C, CXCL12, TOP2A, ALDH1A1 and PRKACB. Therefore, ITIH2 might represent the potential core gene for colon cancer liver metastasis. COL1A2 behaves as a key gene in colorectal carcinoma.


Kerntechnik ◽  
2021 ◽  
Vol 86 (6) ◽  
pp. 454-469
Author(s):  
S. H. Abdel-Latif

Abstract The station black-out (SBO) is one of the main accident sequences to be considered in the field of severe accident research. To evaluate a nuclear power plant’s behavior in the context of this accident, the integral ASTEC-V2.1.1.3 code “Accident Source Term Evaluation Code” covers sequences of SBO accidents that may lead to a severe accident. The aim of this work is to discuss the modelling principles for the core melting and in-vessel melt relocation phenomena of the VVER-1000 reactor. The scenario of SBO is simulated by ASTEC code using its basic modules. Then, the simulation is performed again by the same code after adding and activating the modules; ISODOP, DOSE, CORIUM, and RCSMESH to simulate the ex-vessel melt. The results of the two simulations are compared. As a result of SBO, the active safety systems are not available and have not been able to perform their safety functions that maintain the safety requirements to ensure a secure operation of the nuclear power plant. As a result, the safety requirements will be violated causing the core to heat-up. Moreover potential core degradation will occur. The present study focuses on the reactor pressure vessel failure and relocation of corium into the containment. It also discusses the transfer of Fission Products (FPs) from the reactor to the containment, the time for core heat-up, hydrogen production and the amount of corium at the lower plenum reactor pressure vessel is determined.


2021 ◽  
Vol 931 ◽  
Author(s):  
K.B.M.Q. Zaman ◽  
A.F. Fagan ◽  
P. Upadhyay

An experimental study is conducted on unsteady pressure fluctuations occurring near the nozzle exit and just outside the shear layer of compressible jets. These fluctuations are related to ‘trapped waves’ within the jet's potential core, as investigated and reported recently by other researchers. Round nozzles of three different diameters and rectangular nozzles of various aspect ratios are studied. The fluctuations manifest as a series of peaks in the spectra of the fluctuating pressure. Usually the first peak at the lowest frequency (fundamental) has the highest amplitude and the amplitude decreases progressively for successive peaks at higher frequencies. These ‘trapped wave spectral peaks’ are found to occur with all jets at high subsonic conditions and persist into the supersonic regime. Their characteristics and variations with axial and radial distances, jet Mach number and aspect ratio of the nozzle are documented. For round nozzles, the frequency of the fundamental is found to be independent of the jet's exit boundary layer characteristics and scales with the nozzle diameter. On a Strouhal number (based on diameter) versus jet Mach number plot it is represented by a unique curve. Relative to the fundamental the frequencies of the successive peaks are found to bear the ratios of 5/3, 7/3, 9/3 and so on, at a given Mach number. For rectangular nozzles, the number of peaks observed on the major axis is found to be greater than that observed on the minor axis by a factor approximately equal to the nozzle's aspect ratio; the fundamental is the same on either edge. For all nozzles the onset of screech tones appears as a continuation of the evolution of these peaks; it is as if one of these peaks abruptly increases in amplitude and turns into a screech tone as the jet Mach number is increased.


2021 ◽  
Vol 11 (23) ◽  
pp. 11205
Author(s):  
Cong Yuan ◽  
Lisha Zhu ◽  
Shiqi Liu ◽  
He Li

The higher susceptibility to cavitation in poppet valves due to the lower viscosity of water than the traditionally used mineral oil poses a challenge in fluid transmission technology. To reveal the underlying mechanism of cavitating flow physics associated with the variation in viscosity effect, the current paper examines both the water and oil cavitating flow dynamics inside poppet valves with varied structures through a numerical study. The simulation results are validated with a comparison to previous experimental data in terms of cavitation morphology and pressure distribution. According to the predicted cavitation distribution, three kinds of cavitation occurred at separated positions in both water- and oil-flow cases. The vortex cavitation, which in the oil-flow case displays a remarkable paired structure with favorable coherence, is featured with a scattered dispersion in the water-flow case, while the profound attached cavitation at the poppet trailing edge in the water-flow case almost disappears in the oil-flow case. Furthermore, the attached cavitation within the chamfered groove has higher stability in the oil-flow case, compared to the thorough detachment behavior featured with profound 3-dimensionality in the water-flow case. According to the potential core and vortex evolution, the strong 3-dimensionality due to the violent laminar-turbulent transition in the water-flow case together with the produced puff pattern of the potential core, to a large extent, interrupts the periodic behavior of cavitation, which is essentially preserved in the oil-flow case featured with favorable coherence.


Sign in / Sign up

Export Citation Format

Share Document