scholarly journals Aislamiento e Identificación de bacterias nativas con potencial probiótico en el camarón (Litopenaeus vannamei) cultivado en Honduras, 2018

2019 ◽  
pp. 64-77
Author(s):  
Erick Joaquín Ardón Larios ◽  
Christian Rolando Hernández Coello ◽  
Dunia Yessenia López Sosa ◽  
Liliam Carina Marroquín Tejeda
Keyword(s):  
Bacillus Subtilis ◽  
Litopenaeus Vannamei ◽  
Mueller Hinton

El uso de probióticos se vuelve cada día más común en las producciones acuícolas tanto para la prevención de enfermedades como para el mejoramiento de la calidad de agua y fondos de los sistemas de producción, minimizando el uso indiscriminado de antibióticos, así como mejorando el medio ambiente. Estos probióticos deben adaptarse al medio lo que muchas veces no permite observar resultado favorable a corto plazo. El objetivo fue aislar e Identificar bacterias nativas con características probióticas presentes en el camarón blanco Litopenaeus vannamei el uso de microorganismos nativos nos permitirá optimizar el tiempo de adaptación ya que no tendrán que adaptarse pues vivirán en su hábitat natural. La investigación es descriptiva experimental, se realizaron muestreos aleatorios simples con selección al azar de 60 camarones de cultivo a los cuales se les extrajo el tracto gastrointestinal se maceraron para ser sembraron en medios cultivo selectivos Agar Tiosulfato Citrato Bilis Sacarosa (TCBS), Agar Papa Dextrosa (PDA), Agar MRS para lactobacillus y otras bacterias ácido lácticas, Agar Actinomicetos y Müeller Hinton como medio no selectivo. Los microorganismos que fueron aislados e identificados se llevaron a producción en biorreactor para comprobar las características principales como ser crecimiento rápido y fáciles de cultivar. Se aislaron bacterias de la especie Bacillus subtilis y Actinomicetos las cuales presentan características con potencial probiótico y cumplen con la característica de ser de cultivo poco exigente y con excelente multiplicación.

In vitro and in vivo potential probiotic activity of Bacillus subtilis and Shewanella algae for use in Litopenaeus vannamei rearing

Aquaculture ◽  
2018 ◽  
Vol 488 ◽  
pp. 114-122 ◽  
Author(s):  
Juliana A. Interaminense ◽  
Joana L. Vogeley ◽  
Carolina K. Gouveia ◽  
Rogério W.S. Portela ◽  
José P. Oliveira ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Litopenaeus Vannamei ◽  
Shewanella Algae ◽  
Probiotic Activity

A Study of 3-Substituted Benzylidene-1,3-dihydro-indoline Derivatives as Antimicrobial and Antiviral Agents

2009 ◽  
Vol 64 (3-4) ◽  
pp. 155-162 ◽  
Author(s):  
Süreyya Ölgen ◽  
Semiha Özkan
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Bacillus Subtilis ◽  
Klebsiella Pneumoniae ◽  
Antiviral Agents ◽  
Candida Krusei ◽  
Mueller Hinton ◽  
Antibacterial And Antifungal ◽  
Ampicillin Trihydrate

3-Substituted benzylidene-1,3-dihydro-indoline derivatives were tested for their in vitro antibacterial activity against the Gram-negative bacteria Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli, and the Gram-positive bacteria Bacillus subtilis, Staphylococcus aureus, and for their their in vitro antifungal activity against Candida krusei and Candida albicans. The minimum inhibitory concentration (MIC) values were determined by the 2-fold serial dilution technique in Mueller Hinton broth and Sabouraud dextrose agar using antibacterial and antifungal assays, respectively. For comparison of the antimicrobial activity, rifampicin, ampicillin trihydrate, gentamicin sulfate, and ofloxacin were used as reference antibacterial agents, and fl uconazole and amphotericin B were employed as reference antifungal agents. The most active compound 10 showed notable inhibition against Bacillus subtilis, Staphylococcus aureus, and Candida krusei. Compounds 1 and 6 were found slightly effective against Klebsiella pneumoniae and Escherichia coli. In addition, compounds 13 and 14 showed inhibition against Bacillus subtilis and Staphylococcus aureus. Indole derivatives were also tested in vitro for replication of the HepAD38 cell line and compared with lamivudine (3TC, L-2′,3′-dideoxy-3′-thiacytidine). The IC50 values of the compounds were found to be >1000 μM against HBV except for compound 13 which exhibited activity with an IC50 value of 500 μM.

scholarly journals Budidaya Udang Vannamei (Litopenaeus vannamei) Semiintensif Dengan Metode Sirkulasi Tertutup Untuk Menghindari Serangan Virus [The Semiintensive Culture Of Litopenaeus vannamei By Closed Circulation Method To Prevent Virus Attack]

2019 ◽  
Vol 1 (2) ◽  
pp. 121 ◽  
Author(s):  
Slamet Subyakto, Dede Sutende, Moh. Afandi, Sofiat
Keyword(s):  
Waste Water ◽  
Bacillus Subtilis ◽  
Litopenaeus Vannamei ◽  
Culture Method ◽  
Water Circulation ◽  
Shrimp Culture ◽  
Circulation Method ◽  
Closed Circulation ◽  
Virus Attack ◽  
Additional Water

AbstractThe failure of vannamei shrimp culture often caused by virus attack, i.e. WSSV, TSV and IMNV. So that it need an alternative method like closed circulation method and probiotic application. Closed circulation is culture method without water circulation where as an additional water will do to change loss water, caused by evaporation and culture waste water. This kinds of probiotic ar Bacillus subtilis, Nitrosomonas, Nitrobacter, Saccaromyces, Rhodobacter and Rhodococcus. The aim of these research are preventing virus attack through closed circulation method and probiotic 2 2application. These culture used two culture ponds (3000 m , density 60 species/m ) and two reservoir ponds 2(2000 m ) for 105 days. The first culture pond (A) produced 2895 kg of vannamei shrimp (size 60, SR 96.5 % and FCR 1.3). The second culture pond (B) produced 3025 kg of vannamei shrimp (size 58, SR 97.4 % and FCR 1.28).

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