Extração da proteína e análise dos perfis hemaglutinante e anticoagulante com sangue humano “O” positivo, de frutos da castanhola (Terminalia catappa Linn.)

2021 ◽  
Author(s):  
A. T. C. OLIVEIRA ◽  
F. T. S. SILVA ◽  
L. K. A. R. FILHO ◽  
O. L. OLIVEIRA ◽  
R. C. BRAGA
Keyword(s):  

Nos cotilédones de algumas sementes podem conter proteínas com atividades biológicas específicas, como as lectinas que são capazes de aglutinar eritrócitos, ou inibidores de proteases que agem anticoagulantes naturais que regulam a atividade de proteases. Assim, este trabalho teve por objetivo realizar a extração das proteínas presentes nos cotilédones da semente da castanhola em pHs diferenciados, além de verificar a presença de compostos com atividades anticoagulante e hemaglutinante. Foi então realizada a extração das proteínas, verificando-se a atividade hemaglutinante, anticoagulante e também a concentração de proteínas pelo método do Biureto. Os resultados indicaram não haver a presença de lectinas ou inibidores por não haver atividade coagulante e hemaglutinante nas amostras. Utilizando o método do Biureto foi possível perceber que a concentração de proteína foi baixa, o que pode sugerir que a concentração do extrato poderia não ser suficiente para as atividades testadas, sugerindo-se realizar novamente os testes com maiores concentrações.

2020 ◽  
Vol 10 (4) ◽  
pp. 89-92
Author(s):  
Sravani G ◽  
Linga Naik A ◽  
Kranthi A ◽  
Priyanka G

Plant determined medications remains an important source, particularly in creating countries, to look at genuine sicknesses roughly 62-80% of the total populace although everything depends on conventional medication for the therapy of specific disease. Indeed, plants produce a various scope of bioactive atoms creation them an ironic wellspring of an alternate kind of drugs. There are hardly any reports and utilization of plants in conventional mending by either ancestral individuals or indigenous network. Rejuvenating plants are the wellspring of extraordinary monetary estimation of everywhere on over the world. Nature has the best word on us a rich plant riches, and an enormous number of assorted kinds of plants develop in various pieces of the nation. Homegrown medication is as yet a pillar of around 75 to 85% the entire populace and the significant aspect of the conventional therapy the utilization of the plant extricate and the dynamic constituents. Among the 7000 types of rejuvenating plants perceived everywhere on over the world in excess of 9000, valuable medicinal plants are found in India. Unfortunately, just not many of them are utilized for their therapeutic worth. Around 1500 plants systematically utilize the conventional arrangement of Indian medication. Notwithstanding, the ethanopharmacologist, microbiologist, botanist and common item physicist world over today, is continually still looking for therapeutic adequacy of the plants on the phytochemicals. Along these lines, the quest for the new phytochemical is the foremost significant important to research the primer phytochemical examination to Terminalia catappa and Syzygium jumbolanam.


2018 ◽  
Vol 2 (11) ◽  
pp. 494-497
Author(s):  
Nahandoo Ichôron ◽  
Terrumun Tor-Anyiin ◽  
John Igoli

2017 ◽  
Vol 3 (2) ◽  
Author(s):  
Anna Dhora
Keyword(s):  

Tanaman ketapang (Terminalia catappa L) termasuk  jenis tanaman  yang sangat mudah tumbuh dan terdapat hampir di sebagian besar wilayah Indonesia. Selama ini pemanfaatan tanaman ketapang lebih banyak sebagai tanaman peneduh jalan. Dalam ketapang terkandung zat tanin yang setelah diekstrak dapat dimanfaatkan lebih lanjut. Tanin merupakan salah satu zat yang terdapat dalam tumbuhan dan memiliki banyak manfaat. Adanya tannin dalam beberapa bagian dari tumbuhan, memberikan manfaat tersendiri salah satunya sebagai pelindung bagi tumbuhan tersebut. Manfaat tanin antara lain dipakai dalam industri farmasi, industri pengolahan kulit, industri minuman dan industri pembuatan tinta. Tujuan secara umum yang diinginkan dari dilaksanakannya penelitian ini adalah meningkatkan nilai ekonomis dari tanaman ketapang, serta mengetahui variabel pemakaian pelarut dan waktu ekstraksi yang digunakan agar  memberikan kemampuan maksimal untuk mengekstrak tanin dari daun ketapang. Metode yang diterapkan adalah metode sokletasi pada proses ekstraksi dan metode volumetri untuk proses analisa.  Pelarut yang digunakan adalah etanol dan metanol yang  dikombinasikan dengan aquadest, dengan lama waktu operasi ekstraksi 3 dan 4 jam. Dari penelitian yang telah dilakukan diketahui bahwa pemakaian pelarut metanol dan air dengan perbandingan 6 : 4  dengan waktu ekstraksi 4 jam, menghasilkan kadar tanin tertinggi sebesar 28,90 %. Sedangkan pada pemakaian pelarut campuran etanol dan air, menghasilkan kadar tanin tertinggi pada perbandingan 6 : 4 pada  waktu ekstraksi 4 jam dengan kadar tanin 37, 17.Kata kunci: ketapang, tanin, etanol, metanol sokletasi


2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Ufere N. Uka ◽  
Ebenezer J. D. Belford ◽  
Florence A. Elebe

AbstractThis study was undertaken to examine changes in the content of pigments and accumulation of metals from vehicular pollution in selected species of roadside trees under vehicular pollution. A major arterial road with heavy vehicle emissions in the Kumasi Metropolis was designated as the polluted site, while Kwame Nkrumah University of Science and Technology Campus was designated as the control site. Four tree species (Terminalia catappa, Mangifera indica, Ficus platyphylla and Polyalthia longifolia) selected for the study were well distributed and abundant in the polluted and control sites. Photosynthetic pigments and levels of heavy metals (Pb, Cu, Cd and zinc) were assessed in their leaves. Chlorophyll and carotenoid contents were determined by absorption spectrometry, while the metal accumulation index (MAI) was used to determine the total metal accumulation capacity of the tree species. We observed a reduction in photosynthetic pigments in the leaf samples from the polluted site. Ficus platyphylla had the maximum reduction in total chlorophyll (49.34%), whereas Terminalia catappa recorded the lowest reduction (33.88%). Similarly, the largest decrease (31.58%) of carotenoid content was found in Terminalia catappa trees and the lowest in Polyalthia longifolia (16.67%). The Polyalthia longifolia, Ficus platyphylla and Terminalia catappa leaf samples collected at the polluted site recorded a higher ratio of chlorophyll a/b. Heavy metal (Cu, Pb, Zn and Cd) accumulation in leaf samples was higher in the polluted site than in the control, as expected. The highest metal MAI value was recorded in Mangifera indica (5.35) followed by Polyalthia longifolia with 4.30. The findings from this study specifically demonstrate that air contamination induced by vehicles decreases the level of photosynthetic pigments in trees subjected to roadside emissions. It is clear that both chlorophyll a/b and chlorophyll/carotenoid ratios will act as very useful stress-level markers. Elevated heavy metal levels in the tree species along arterial roadsides indicate that they serve as heavy metals sink. The change in MAI resulting from different pollution burden is an indication that the removal capabilities of the tree species differ from each other. We therefore suggest M. indica and P. longifolia as potential species to be used in air pollution reduction plans in the city.


Aquaculture ◽  
2021 ◽  
pp. 736914
Author(s):  
Juliana Oliveira Meneses ◽  
Izadora Cibely Alves da Silva ◽  
Ana Flávia Santos da Cunha ◽  
João Carlos Nunes de Souza ◽  
Victor Ruan Silva Nascimento ◽  
...  

Author(s):  
Folashade Sarah Ojeleye ◽  
Helen Ileigo Inabo ◽  
Clement Myah Zaman Whong ◽  
Bolanle Olufunke Priscilla Musa ◽  
Ochuko Orakpoghenor

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