scholarly journals Lactic Acid Bacteria Enriched from Human Gastric Biopsies

2011 ◽  
Vol 2011 ◽  
pp. 1-4 ◽  
Author(s):  
Elias Hakalehto ◽  
Terttu Vilpponen-Salmela ◽  
Kristiina Kinnunen ◽  
Atte von Wright
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Microbial Growth ◽  
Lactobacillus Reuteri ◽  
Common Species ◽  
Streptococcus Salivarius ◽  
Food Applications ◽  
Viable Bacteria ◽  
Gastric Biopsies ◽  
Continuous Presence

The purpose of this paper was to check if viable bacteria, in particular lactic acid bacteria (LAB), could be enriched from biopsies obtained from healthy gastroscopy patients. Gastric biopsies were obtained from 13 gastroscopy patients and subjected to an anaerobic or microaerophilic enrichment procedure utilizing the Portable Microbe Enrichment Unit (PMEU). Profuse microbial growth was observed in most cases. Samples plated on MRS showed high numbers of LAB. The most common species characterized were Lactobacillus reuteri, Lact. salivarius, and Streptococcus salivarius. The results demonstrate a continuous presence of viable LAB in healthy stomach. The species are similar to those traditionally used in food applications. The gastric LAB strains could have a potential in developing probiotic foods aimed specially on the upper part of the gastrointestinal tract.

scholarly journals Complete Genome Sequence of Lactobacillus reuteri Byun-re-01, Isolated from Mouse Small Intestine

2018 ◽  
Vol 7 (17) ◽  
Author(s):  
Dongjun Kim ◽  
Mun-ju Cho ◽  
Seungchan Cho ◽  
Yongjun Lee ◽  
Sung June Byun ◽  
...  
Keyword(s):  
Small Intestine ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Lactobacillus Reuteri ◽  
Probiotic Bacteria ◽  
Content Type ◽  
Mouse Small Intestine

Lactic acid bacteria (LAB) are generally recognized as safe (GRAS) and serve as probiotic bacteria when consumed in adequate amounts. Here, we report the complete genome sequence of Lactobacillus reuteri Byun-re-01, isolated from mouse small intestine.

scholarly journals MANFAAT KESEHATAN BAKTERI ASAM LAKTAT

2019 ◽  
Vol 2 (1) ◽  
pp. 54-60
Author(s):  
Siti Nur Purwandhani
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Casei ◽  
Nutritional Value ◽  
Intestinal Microflora ◽  
Lactobacillus Reuteri ◽  
Health Benefits ◽  
Vitamin B ◽  
Antimicrobial Substances ◽  
Cell Components

Lactic acid bacteria has potential as health-supporting agent because of its role as probiotic that has capability to grow in gastrointestinal tract, and its metabolites/cell components that have health benefits. Health benefits of lactic acid bacteria include balancing intestinal microflora so it can play a role in treating diarrhea, preventing cancer, lowering serum cholesterol, treating lactose intolerant, and preventing constipation. In terms of nutritional value, lactic acid bacteria is capable of increasing the biological value of milk protein, synthesizing vitamin B, inhibiting antinutrient compound, inhibiting toxins production, and producing antimicrobial substances. Examples of lactic acid bacteria strain that can be used as a probiotic are Lactobacillus reuteri, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus casei.

scholarly journals Isolation and characterization of lactic acid bacteria from Sabah (North Borneo) stingless bees for probiotic and food applications

2021 ◽  
Vol 24 (1) ◽  
pp. 564-578
Author(s):  
Lucky Poh Wah Goh ◽  
Arnold Marshall Molujin ◽  
Kaliswaran Muthu ◽  
Rahmath Abdulla ◽  
Mohd Khalizan Sabullah ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Stingless Bees ◽  
Isolation And Characterization ◽  
North Borneo ◽  
Food Applications

scholarly journals Inhibition of Staphylococcus aureus Growth on Tellurite-Containing Media by Lactobacillus reuteri Is Dependent on CyuC and Thiol Production

2006 ◽  
Vol 73 (3) ◽  
pp. 1005-1009 ◽  
Author(s):  
Mark S. Turner ◽  
Raquel Lo ◽  
Philip M. Giffard
Keyword(s):  
Staphylococcus Aureus ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Lactobacillus Reuteri

ABSTRACT Lactobacillus reuteri inhibits Staphylococcus aureus growth on Baird-Parker agar. This activity required the presence of tellurite and was not shared with other lactic acid bacteria or an L. reuteri mutant defective in cystine metabolism. Secreted products generated from L. reuteri cystine metabolism and thiols were shown to augment tellurite toxicity.

scholarly journals Polyphasic Screening, Homopolysaccharide Composition, and Viscoelastic Behavior of Wheat Sourdough from a Leuconostoc lactis and Lactobacillus curvatus Exopolysaccharide-Producing Starter Culture

2012 ◽  
Vol 78 (8) ◽  
pp. 2737-2747 ◽  
Author(s):  
Simona Palomba ◽  
Silvana Cavella ◽  
Elena Torrieri ◽  
Alessandro Piccolo ◽  
Pierluigi Mazzei ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Viscoelastic Properties ◽  
Microbial Growth ◽  
Starter Culture ◽  
Proton Nuclear Magnetic Resonance ◽  
Lactobacillus Curvatus ◽  
Fermentation Time ◽  
Content Type ◽  
Leuconostoc Lactis

ABSTRACTAfter isolation from different doughs and sourdoughs, 177 strains of lactic acid bacteria were screened at the phenotypic level for exopolysaccharide production on media containing different carbohydrate sources. Two exopolysaccharide-producing lactic acid bacteria (Lactobacillus curvatus69B2 andLeuconostoc lactis95A) were selected through quantitative analysis on solid media containing sucrose and yeast extract. The PCR detection of homopolysaccharide (gtfandlev) and heteropolysaccharide (epsA,epsB,epsDandepsE, andepsEFG) genes showed different distributions within species and strains of the lactic acid bacteria studied. Moreover, in some strains both homopolysaccharide and heteropolysaccharide genes were detected. Proton nuclear magnetic resonance spectra suggest thatLactobacillus curvatus69B2 andLeuconostoc lactis95A produced the same exopolysaccharide, which was constituted by a single repeating glucopyranosyl unit linked by an α-(1→6) glycosidic bond in a dextran-type carbohydrate. Microbial growth, acidification, and viscoelastic properties of sourdoughs obtained by exopolysaccharide-producing and nonproducing lactic acid bacterial strains were evaluated. Sourdough obtained after 15 h at 30°C with exopolysaccharide-producing lactic acid bacteria reached higher total titratable acidity as well as elastic and dissipative modulus curves with respect to the starter not producing exopolysaccharide, but they showed similar levels of pH and microbial growth. On increasing the fermentation time, no difference in the viscoelastic properties of exopolysaccharide-producing and nonproducing samples was observed. This study suggests that dextran-producingLeuconostoc lactis95A andLactobacillus curvatus69B2 can be employed to prepare sourdough, and this would be particularly useful to improve the quality of baked goods while avoiding the use of commercially available hydrocolloids as texturizing additives.

scholarly journals Use of metabolites of therapeutic Lactic acid bacteria to inhibit four species veterinary pathogenic bacteria

2005 ◽  
Vol 29 (2) ◽  
pp. 49-55
Author(s):  
Ali H. A . Hamid
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Pathogenic Bacteria ◽  
Lactobacillus Reuteri ◽  
Inhibition Zone ◽  
Culture Cell ◽  
Inhibition Activity ◽  
Inhibition Zone Diameter ◽  
Zone Diameter ◽  
Mrs Broth

This study aimed to detect metabolites inhibition activity for three therapeuticLactic acid bacteria Lactobacillus acidophilus (Lb. acid.) , Lactobacillus reuteri(Lb. reut.) and Bifidobacterium ssp. (Bif.) which were grown in whole milk andMan Reqosa and Sharp (MRS) broth as single or double (Lb. acid. + Bif.) ormixed (Lb. reut + Bif. + Lb. acid) culture Cell Free Extract (CFE) of theseculture which contained their metabolites of Lactic acid bacteria (exclusion Lb.reut.) showed good inhibition against four species of pathogenic Escherichiacoli ; Salmonella typhimurium ; Proteus volgaris and Staphylococcus aureus.inhibition activity against E. coli culture showed that 72 hours was the bestincubation time for Lactic acid bacteria to produce more metabolite with bestinhibition activity. Metabolite (CFE) of Lb. Acid culture grown in milk andMRS broth showed the best inhibition activity against all of Gram Positive andGram negative test bacteria with over all inhibition zone diameter was 14.6 and15.3 mm for milk and MRS broth supernatant respectively and this activityremained for more than one week as inhibition zone diameter were 13.4 and14.5 mm for supernatant of milk and MRS respectively.

In vitro assessment of functional properties of lactic acid bacteria isolated from faecal microbiota of healthy dogs for potential use as probiotics

2013 ◽  
Vol 4 (3) ◽  
pp. 267-275 ◽  
Author(s):  
B.C. Silva ◽  
L.R.C. Jung ◽  
S.H.C. Sandes ◽  
L.B. Alvim ◽  
M.R.Q. Bomfim ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bile Salts ◽  
Pathogenic Bacteria ◽  
Lactobacillus Reuteri ◽  
Cell Surface Hydrophobicity ◽  
Lactobacillus Paraplantarum ◽  
Production Of Hydrogen

Lactic acid bacteria were isolated and identified in the faeces of Chinese Crested and Yorkshire terrier pups and their probiotic features were investigated in vitro. Thirty seven isolates were identified as Lactobacillus or Enterococcus. Out of these isolates, 31 were lactic acid bacteria (LAB) and belonged to the species Lactobacillus reuteri (16/37; 43.3%), Lactobacillus animalis (7/37; 18.9%), Lactobacillus acidophilus (3/37; 8.1%), Lactobacillus sanfranciscensis (2/37; 5.4%), Lactobacillus murinus (2/37; 5.4%), and Lactobacillus paraplantarum (1/37; 2.7%), while six other LAB isolates were Enterococcus spp. (6/37; 16.2%). Strains were tested for resistance to gastric acidity (pH 2.5 for 3 h) and bile salts (0.3% ox gall), cell surface hydrophobicity by microbial adhesion to solvents, antagonism against pathogenic bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus cereus, Pseudomonas aeruginosa, Escherichia coli, Salmonella enterica serovar Typhimurium and Listeria monocytogenes), production of hydrogen peroxide, and antibiotic susceptibility. Thirty four strains were highly resistant to acidic conditions with slight (18 strains) to moderate (16 strains) growth inhibition by bile salts. Seven isolates had highly hydrophobic cellular surfaces and 28 strains exhibited strong antagonism against the bacterial pathogens tested, although 8 isolates tested against Leptospira interrogans had no effect on pathogen growth. All isolates produced low rates of hydrogen peroxide. Based on these results, two Lactobacillus strains showed promising probiotic-related features and merit investigation as probiotics for dogs.

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