scholarly journals Simultaneous Estimation of Lafutidine and Domperidone by Ultraviolet Spectroscopy

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Kiran Jadhav ◽  
D. L. Dhamecha ◽  
S. V. Ghadlinge ◽  
G. P. Asnani ◽  
M. B. Patil
Keyword(s):  
Standard Deviation ◽  
Simultaneous Determination ◽  
Dosage Form ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Ultraviolet Spectroscopy ◽  
Precise Method

A simple, accurate, and precise method for simultaneous estimation of Lafutidine and Domperidone in combined-dosage form have been described. The method employs formation and solving of simultaneous equations using 279 and 284 nm as two analytical wavelengths. This method allows the simultaneous determination of Lafutidine and Domperidone in concentration ranges employed for this purpose with the standard deviation of <1.0% in the assay of tablet.

scholarly journals Simultaneous UV Spectrophotometric Determination of Cetrizine and Dextromethorphan in Tablet Dosage Form

2010 ◽  
Vol 7 (s1) ◽  
pp. S314-S318 ◽  
Author(s):  
R. Vijayalakshmi ◽  
S. Bhargavi ◽  
M. D. Dhanaraju
Keyword(s):  
Standard Deviation ◽  
Spectrophotometric Determination ◽  
Dosage Form ◽  
Dosage Forms ◽  
Simultaneous Equations ◽  
Simultaneous Estimation ◽  
Isobestic Point ◽  
Ich Guidelines ◽  
Tablet Dosage

Two accurate, precise, sensitve and economical procedures for simultaneous estimation of cetrizine and dextromethorphan in tablet dosage forms have been developed. First method employs formation and solving of simultaneous equations using 230 nm and 280 nm as two analytical wavelengths for both drugs in methanol. The second method isQ-analysis based on measurement of absorptivity at 224 nm (as isobestic point) and 280 nm (λmaxof CTZ). Cetrizine and dextromethorphan at their respective λmax280 nm and 230 nm and at 224 nm (isobestic point) shows linearity in a concentration range of 10-30 mcg/mL for both the drugs. The recovery studies confirmed accuracy of the proposed methods and low values of standard deviation confirmed precision of the methods. The methods were validated as per ICH guidelines.

DEVELOPMENT AND VALIDATION OF NEW HPT LC METHOD FOR SIMULTANEOUS ESTIMATION OF RUPATIDINE FUMARATE AND MONTELUKAST SODIUM IN TABLET DOSAGE FORM

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (06) ◽  
pp. 29-35
Author(s):  
T Raja ◽  
◽  
A. L Rao
Keyword(s):  
Simultaneous Determination ◽  
High Performance ◽  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Solvent System ◽  
Simultaneous Estimation ◽  
Montelukast Sodium ◽  
Aluminum Plates ◽  
Tablet Dosage

A new simple high performance thin layer chromatographic method for simultaneous determination of rupatidine fumarate and montelukast sodium in bulk and tablet dosage form was investigated. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F254 as the stationary phase and the solvent system consisted of acetone:methanol:toluene (2:3:5, V/V/V). Densitometric evaluation of the separated zones was performed at 286 nm and the method was validated. The Rf values and drug content of rupatidine fumarate and montelukast sodium were 0.57±0.02, 0.68±0.02 and 98.3%, 97.67% respectively. The calibration curves of peak area versus concentration, were linear from 50-300 ng per band for both rupatidine fumarate and montelukast sodium and the regression coefficient (r2 ) was greater than 0.99. The method was validated for linearity, accuracy, robustness and application for assay as per ICH guidelines. The study showed that the developed method was simple and accurate and would be suitable for the simultaneous determination of rupatidine fumarate and montelukast sodium in bulk and pharmaceutical formulations.

scholarly journals Stability-indicating RP-HPLC method for simultaneous estimation of levosalbutamol sulfate and theophylline in combined dosage form

2013 ◽  
Vol 49 (3) ◽  
pp. 475-490 ◽  
Author(s):  
Sagar Suman Panda ◽  
Bera Venkata Varaha Ravi Kumar ◽  
Ganeswar Mohanta
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Rp Hplc ◽  
Photolytic Degradation ◽  
Tetrabutyl Ammonium ◽  
System Suitability ◽  
Ammonium Hydrogen

A novel, simple, accurate and precise RP-HPLC method for simultaneous determination of levosalbutamol sulfate and theophylline has been developed and validated. Separation was achieved on a Phenomenex; C18 column (250 mm × 4.6 mm i.d., 5 µm) using methanol: 10 mM TBAHS(tetrabutyl ammonium hydrogen sulfate) (50:50, v/v) as mobile phase at flow rate of 1.0 mL.min-1. The UV detection wavelength was 274 nm. The linearity is obeyed over a concentration range of 0.5-150 µg.mL-1 with correlation coefficient of 0.999 for both the drugs. The proposed method was validated by determining accuracy, precision, stability and system suitability parameters. The method was found to be robust. Specificity of the method was determined by subjecting the drugs to various stress conditions like acid, alkali, oxidation, thermal and photolytic degradation. The method was used successfully for the simultaneous determination of levosalbutamol sulfate and theophylline in syrup dosage form.

scholarly journals DEVELOPMENT AND VALIDATION OF STABILITY INDICATING SIMULTANEOUS UVSPECTROPHOTOMETRIC METHOD FOR DETERMINATION OF EMTRICITABINE, TENOFOVIR DISOPROXIL FUMARATE, COBICISTAT, AND ELVITEGRAVIR IN PURE AND PHARMACEUTICAL DOSAGE FORM

2018 ◽  
Vol 11 (4) ◽  
pp. 177 ◽  
Author(s):  
Harini U ◽  
Pawar Akm
Keyword(s):  
Simultaneous Determination ◽  
Tenofovir Disoproxil Fumarate ◽  
Dosage Form ◽  
Research Work ◽  
Simultaneous Equation ◽  
Base Hydrolysis ◽  
Simultaneous Estimation ◽  
Forced Degradation ◽  
Pharmaceutical Dosage Form

 Objective: The objective of this research work was to develop and validate a simple, precise, and accurate new ultraviolet-spectrophotometric method for the simultaneous estimation of emtricitabine (EMT), tenofovir disoproxil fumarate (TDF), cobicistat (COB), and elvitegravir (ELV) in pure and pharmaceutical marketed dosage form (stribild).Methods: Simultaneous equation method (Vierordt’s method) was developed for simultaneous determination of several mixtures containing two or more absorbing drugs (p, q, r, and s) each of which absorbs at the λ max of the other. Forced degradation studies were also conducted, and the drugs were subjected to various stress conditions such as acid hydrolysis, base hydrolysis, oxidative, photolytic, and thermal degradation.Results: The λ max of EMT, ten TDF, COB, and ELV are 283 nm, 259 nm, 240 nm, and 258 nm, respectively. The linearity ranges for the four drugs are EMT (4–24 μg/ml), TDF (10–50 μg/ml), COB (10–120 μg/ml), and ELV (2–10 μg/ml).Conclusion: The Vierordt’s method was successfully applied for simultaneous determination of EMT, TDF, COB, and ELV in a mixture of sample solution (pharmaceutical dosage form) and the results obtained were validated and found to be accurate, precise, linear, rugged, and robust.

APPLICATION OF RATIO DERIVATIVE SPECTROPHOTOMETRIC METHOD FOR SIMULTANEOUS DETERMINATION OF ARTEMISININ AND CURCUMIN

INDIAN DRUGS ◽  
2015 ◽  
Vol 52 (08) ◽  
pp. 43-47
Author(s):  
R Dhankecha ◽  
◽  
A Soni ◽  
M Gohel ◽  
V Thakkar ◽  
...  
Keyword(s):  
Simultaneous Determination ◽  
Absorption Spectra ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Estimation ◽  
Direct Absorption ◽  
Acceptable Limit ◽  
First Derivative ◽  
Ich Guidelines

Ratio derivative spectrophotometric method has been developed for the simultaneous determination of artemisinin and curcumin. The overlapping spectra of both drugs were resolved by making use of the first-derivative of the ratios of their direct absorption spectra. The derivative ratio absorbances of artemisinin and curcumin were measured at λmax 293.17 and λmax 457.0 nm, respectively, for their quantification. Artemisinin and curcumin were determined in the concentration range of 2-10 μg/mL and 1-5 μg/mL, respectively. The method was validated as per the ICH guidelines and accuracy, precision were found to be within acceptable limit. The limits of detection and quantitation were found to be 0.003299 and 0.009997 μg/mL, respectively for artemisinin and 0.006743 and 0.020434 μg/mL, respectively for curcumin. The proposed ratio first derivative spectrophotometric method is novel, rapid, simple, sensitive, accurate, precise and successfully applicable for simultaneous estimation of artemisinin and curcumin in parentral dosage form.

scholarly journals Development and validation of new analytical method for the estimation of fluoxetine in bulk and dosage form by UV spectrophotometry

2019 ◽  
Vol 1 (2) ◽  
pp. 36-39
Author(s):  
Uday sankar raju K ◽  
Vimalakkannan T ◽  
K. Ravindra Reddy ◽  
P. Naveena ◽  
R. Riharika raj ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Quantitative Determination ◽  
Dosage Form ◽  
Dosage Forms ◽  
Uv Spectrophotometry ◽  
Pharmaceutical Dosage Forms ◽  
Precise Method ◽  
Absorbance Measurement ◽  
Development And Validation

A simple, rapid and precise method is developed for the quantitative determination of Fluoxetine in combined pharmaceutical-dosage forms. The method was based on UV Spectrophotometric determination of Fluoxetine drug using Beer-Lamberts Law. It involves absorbance measurement at 224 nm (λmax of Fluoxetine) in water. For UV Spectrophotometric method, linearity was obtained in concentration range of 5-30 mcg/ml with regression 0.999 for Fluoxetine respectively. Recovery was in the range of 98 -102%; the value of standard deviation and %R.S.D was found to be < 2 shows high precision of the method..

Spectrophotometric Estimation of Rifampin-Isoniazid Mixture in Dosage Form

1987 ◽  
Vol 70 (4) ◽  
pp. 679-681
Author(s):  
Suresh C Sharma ◽  
Sukomal Das ◽  
Santosh K Talwar
Keyword(s):  
Simultaneous Determination ◽  
Spectrophotometric Method ◽  
Dosage Form ◽  
Simultaneous Equations

Abstract A spectrophotometric method for the simultaneous determination of rifampin and isoniazid in dosage form is described. By using a mixtore of methanol and water (2 + 3) as solvent, the absorbance of the mixtures of rifampin and isoniazid is measured at 264 nm and 335 am. The concentration of each can then be calculated by solving 2 simultaneous equations. The procedure was successfully applied to a number of commercial samples; the results agreed well with those for compendial method.

scholarly journals A new analytical method for determination of ledipasvir and sofosbuvir in pharmaceutical formulations by HPLC method

2019 ◽  
Vol 1 (3) ◽  
pp. 59-67
Author(s):  
K. Swathi ◽  
P. Venkateswara Rao ◽  
N. Srinivasa Rao
Keyword(s):  
Mobile Phase ◽  
Dosage Form ◽  
Pharmaceutical Formulations ◽  
Hplc Method ◽  
Simultaneous Estimation ◽  
Regression Equations ◽  
Precise Method ◽  
Tablet Dosage ◽  
Quality Control Test

A simple, Accurate, precise method was developed for the simultaneous estimation of the Sofosbuvir and Ledipasvir in Tablet dosage form. Chromatogram was run through Std Discovery C8 150 x 4.6 mm, 5m. Mobile phase containing Buffer 0.1% OPA: Acetonitrile taken in the ratio 60:40 was pumped through column at a flow rate of 1 ml/min. Buffer used in this method was 0.1% OPA buffer. Temperature was maintained at 30°C. Optimized wavelength selected was 260 nm. Retention time of Sofosbuvir and Ledipasvir were found to be 2.367 min and 3.436 min. %RSD of the Sofosbuvir and Ledipasvir were and found to be 0.6 and 0.5 respectively. %Recovery was obtained as 99.61% and 99.80% for Sofosbuvir and Ledipasvir respectively. LOD, LOQ values obtained from regression equations of Sofosbuvir and Ledipasvir were 0.67, 2.02 and 0.70, 2.12 respectively. Regression equation of Sofosbuvir is y = 4266.x + 7700, and y = 4861.x + 2656.of Ledipasvir. Retention times were decreased and run time was decreased, so the method developed was simple and economical that can be adopted in regular Quality control test in Industries.

Comparison of Chemometric assisted UV Spectrophotometric and RP-HPLC Method for the simultaneous determination of Ofloxacin and Tinidazole in their Combined dosage form

2021 ◽  
pp. 5713-5718
Author(s):  
Santosh V. Gandhi ◽  
Deepak Patil ◽  
Atul A. Baravkar
Keyword(s):  
Simultaneous Determination ◽  
Dosage Form ◽  
Hplc Method ◽  
Partial Least Square ◽  
Least Square ◽  
Partial Least Square Regression ◽  
Simultaneous Estimation ◽  
Pharmaceutical Dosage Form ◽  
Rp Hplc

In present work, chemometric-assisted UV spectrophotometric methods as well as RP-HPLC method were developed for the simultaneous estimation of Ofloxacin and Tinidazole in their combined pharmaceutical dosage form. The two chemometric methods i.e. principle component regression (PCR) and partial least square regression (PLS) were successfully applied to quantify each drug in mixture using UV absorption spectra in range of 280 to 320nm at ∆λ of 0.5nm. Chemometric model development was done using 24 binary mixture solutions and 12 solutions were used for validation of model. The chemometric-assisted analysis does not require any prior separation step. In addition, RP-HPLC method was also developed using THERMOSIL C18 column with a mobile phase consisting ofAcetonitrile: Phosphate Buffer (85:15% v/v), flow rate of 1 ml/min and quantification was achieved using UV detector at 300 nm. The methods were successfully applied for the simultaneous determination of these drugs in synthetic mixture. The results obtained for analysis by PCR and PLS methods were compared with RP-HPLC method and a good agreement was found.

A Green Liquid Chromatographic Method For The Simultaneous Determination of Chlorpheniramine Maleate, Pseudoephedrine Hydrochloride and Propyphenazone

2019 ◽  
Vol 15 (6) ◽  
pp. 635-641
Author(s):  
Nadia M. Mostafa ◽  
Ghada M. Elsayed ◽  
Nagiba Y. Hassan ◽  
Dina A. El Mously
Keyword(s):  
Simultaneous Determination ◽  
Mobile Phase ◽  
Dosage Form ◽  
Chromatographic Method ◽  
Green Analytical Chemistry ◽  
Chlorpheniramine Maleate ◽  
Accuracy And Precision ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic

Background:The concept of green analytical chemistry prevails due to the growing environmental pollution.Objective:Our attempts are to develop simple and eco-friendly method which is non-harmful to the environment by producing minimal waste. In this context, a green liquid chromatographic method was applied for the simultaneous determination of chlorpheniramine maleate, pseudoephedrine hydrochloride and propyphenazone in their combined dosage form.Methods:Separation was carried out using X select HSS RP C18 analytical column (250 × 4.6 mm, 5μm) using methanol - 0.02 M phosphate buffer pH 3 - triethylamine (60:40: 0.1, by volume) as a mobile phase. The separated peaks were detected at 215 nm at a flow rate 1.0 mL/min.Results:Quantification was done over the concentration ranges of 1-25 µg/mL for chlorpheniramine maleate, 5-35 µg/mL for pseudoephedrine hydrochloride and 10-120 µg/mL for propyphenazone. The suggested method was validated with regard to linearity, accuracy and precision according to the International Conference on Harmonization guidelines with good results.Conclusion:It could be used as a safer alternative for routine analysis of the mentioned drugs in quality control laboratories.

Sign in / Sign up

Export Citation Format

Share Document