Evaluation of Tomato spotted wilt virus-GT Tolerance in Tomato Cultivars

Isolates of Tomato spotted wilt virus (TSWV) severely and systemically infecting commercial pepper cultivars with resistance introgressed from Capsicum chinense PI152225 were found in Albenga (northwestern Italy) in July 2000. Experimentally, these resistance-breaking (RB) isolates overcame the resistance in C. chinense PI152225, but they produced infection in other hosts similarly to non-RB isolates from the same area. The RB isolates were indistinguishable from TSWV by serology and electron microscopy, and they were efficiently transmitted by Frankliniella occidentalis. Such isolates were recovered on the same farm in tomato, pepper, and artichoke 2 and 12 months later, suggesting natural spread from the resistant plants and survival. The RB isolates survived in experimental mixed infections with a non-RB isolate in susceptible pepper and C. chinense, but cross-protection in pepper acted against them. Commercial TSWV-resistant pepper but not resistant tomato cultivars from different companies were susceptible to these RB isolates after mechanical inoculation. Similar isolates were not detected among TSWV samples collected from 1993 to 2000 in the area. The management of TSWV and thrips using resistant pepper cultivars is discussed.

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Host Plant Resistance to Tomato spotted wilt virus (Bunyaviridae: Tospovirus) in Tomato

HortScience ◽

10.21273/hortsci.46.12.1626 ◽

2011 ◽

Vol 46 (12) ◽

pp. 1626-1633 ◽

Cited By ~ 9

Author(s):

David G. Riley ◽

Shimat V. Joseph ◽

W. Terry Kelley ◽

Steve Olson ◽

John Scott

Keyword(s):

Tomato Spotted Wilt Virus ◽

Frankliniella Occidentalis ◽

Western Flower Thrips ◽

Enzyme Linked Immunosorbent Assay ◽

Plastic Mulch ◽

Flower Thrips ◽

Tomato Spotted Wilt ◽

Tomato Cultivars ◽

Black Plastic ◽

Wilt Virus

Commercially available cultivars of tomato Solanum lycopersicum L. were field-tested for resistance to Tomato spotted wilt virus (TSWV) over a 5-year period (from 2006 to 2010) at the Coastal Plain Experiment Station at Tifton, GA. Selected cultivars were transplanted each year into staked, black plastic mulch beds on drip irrigation in the spring of each year when the incidence of Tomato spotted wilt (TSW) tended to be highest. The presence of TSWV was confirmed by double antibody sandwich (DAS) enzyme-linked immunosorbent assay (ELISA). Also, the presence of thrips vectors was monitored using beat-cup sampling of foliage and flower samples. Tomato cultivars with the Sw-5 resistance gene provided high levels of control of TSW expression over all 5 years. However, these genotypes had no apparent effect on the thrips vectors, western flower thrips, Frankliniella occidentalis (Pergande), and tobacco thrips, Frankliniella fusca (Hinds), that transmit TSWV. Overall, the top 15 commercial tomato cultivars based on consistent TSW resistance and ranked from highest marketable fruit yield were: ‘Tycoon’, ‘Tous 91’, ‘Talladega’, ‘Red Defender’, ‘BHN 444’, ‘Nico’, ‘Carson’, ‘BHN 685 (Roma type)’, ‘Picus’, ‘Redline’, ‘Tribute’, ‘Quincy’, ‘BHN 640’, ‘BHN 602’, and ‘Top Gun’.

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Modification of thrips feeding behaviors by tomato spotted wilt virus

10.1603/ice.2016.92252 ◽

2016 ◽

Author(s):

Diane E. Ullman

Keyword(s):

Tomato Spotted Wilt Virus ◽

Feeding Behaviors ◽

Tomato Spotted Wilt ◽

Wilt Virus

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Current Status and Potential of RNA Interference for the Management of Tomato Spotted Wilt Virus and Thrips Vectors

Pathogens ◽

10.3390/pathogens10030320 ◽

2021 ◽

Vol 10 (3) ◽

pp. 320

Author(s):

Alexander Nilon ◽

Karl Robinson ◽

Hanu R. Pappu ◽

Neena Mitter

Keyword(s):

Rna Interference ◽

Tomato Spotted Wilt Virus ◽

Cultural Practices ◽

Management Strategies ◽

Current Status ◽

Horticultural Crops ◽

Yield And Quality ◽

Tomato Spotted Wilt ◽

Wilt Virus

Tomato spotted wilt virus (TSWV) is the type member of the genus Orthotospovirus in the family Tospoviridae and order Bunyavirales. TSWV, transmitted by several species of thrips, causes significant disease losses to agronomic and horticultural crops worldwide, impacting both the yield and quality of the produce. Management strategies include growing virus-resistant cultivars, cultural practices, and managing thrips vectors through pesticide application. However, numerous studies have reported that TSWV isolates can overcome host-plant resistance, while thrips are developing resistance to pesticides that were once effective. RNA interference (RNAi) offers a means of host defence by using double-stranded (ds) RNA to initiate gene silencing against invading viruses. However, adoption of this approach requires production and use of transgenic plants and thus limits the practical application of RNAi against TSWV and other viruses. To fully utilize the potential of RNAi for virus management at the field level, new and novel approaches are needed. In this review, we summarize RNAi and highlight the potential of topical or exogenous application of RNAi triggers for managing TSWV and thrips vectors.

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