Production of citric and isocitric acid by Yarrowia lipolytica strains grown on different carbon sources

2014 ◽  
Vol 39 (3) ◽  
pp. 285-290 ◽  
Author(s):  
Fusun Bahriye Ucar ◽  
Gonul Celik ◽  
Onur Akpinar ◽  
Cengiz Corbaci
Keyword(s):  
Yarrowia Lipolytica ◽  
Carbon Sources ◽  
Isocitric Acid

scholarly journals Effect of Metabolic Regulators and Aeration on Isocitric Acid Synthesis by Yarrowia lipolytica Grown on Ester-Aldehyde Fraction

Fermentation ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. 283
Author(s):  
Svetlana V. Kamzolova ◽  
Igor G. Morgunov
Keyword(s):  
Yarrowia Lipolytica ◽  
Itaconic Acid ◽  
Isocitrate Lyase ◽  
Nitrogen Deficiency ◽  
Carbon Sources ◽  
Acid Synthesis ◽  
Aconitate Hydratase ◽  
Isocitric Acid ◽  
Metabolic Regulators ◽  
Putative Mechanism

Isocitric acid (ICA) has found wide application in medicine as a promising compound with powerful antioxidant activity to combat oxidative stress. In the known microbiological processes of ICA production by non-conventional yeast Yarrowia lipolytica, the pure carbon sources are commonly used. ICA can be also synthetized by Y. lipolytica from ester-aldehyde fraction (EAF)-waste of the ethanol production process. A highly effective method of ICA production from EAF based on regulation of key enzymes (aconitate hydratase and isocitrate lyase) by metabolic regulators (iron and itaconic acid) and aeration was developed. It is recommended to cultivate Y. lipolytica VKM Y-2373 under nitrogen deficiency conditions, a high aeration (60% of air saturation), an addition of 15 mM itaconic acid, and 2.4 mg/L iron. Under optimal conditions, Y. lipolytica VKM Y-2373 produced 83 g/L ICA with isocitrate to citrate ratio of 4.1:1 and mass yield of 1.1 g/g. The putative mechanism of ICA overproduction from EAF by Y. lipolytica was suggested.

Metabolome analysis and pathway abundance profiling of Yarrowia lipolytica cultivated on different carbon sources

2015 ◽  
Vol 206 ◽  
pp. 42-51 ◽  
Author(s):  
Chen Zhao ◽  
Deqing Gu ◽  
Komi Nambou ◽  
Liujing Wei ◽  
Jun Chen ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Carbon Sources ◽  
Metabolome Analysis

scholarly journals The Gluconeogenic Enzyme Fructose-1,6-Bisphosphatase Is Dispensable for Growth of the Yeast Yarrowia lipolytica in Gluconeogenic Substrates

2008 ◽  
Vol 7 (10) ◽  
pp. 1742-1749 ◽  
Author(s):  
Raquel Jardón ◽  
Carlos Gancedo ◽  
Carmen-Lisset Flores
Keyword(s):  
Yarrowia Lipolytica ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Isocitrate Lyase ◽  
Carbon Sources ◽  
Subcellular Fractionation ◽  
Fructose 1,6 Bisphosphatase ◽  
Genes Encoding ◽  
Key Enzyme ◽  
Gluconeogenic Enzyme ◽  
Fructose 1,6 Bisphosphate

ABSTRACT The genes encoding gluconeogenic enzymes in the nonconventional yeast Yarrowia lipolytica were found to be differentially regulated. The expression of Y. lipolytica FBP1 (YlFBP1) encoding the key enzyme fructose-1,6-bisphosphatase was not repressed by glucose in contrast with the situation in other yeasts; however, this sugar markedly repressed the expression of YlPCK1, encoding phosphoenolpyruvate carboxykinase, and YlICL1, encoding isocitrate lyase. We constructed Y. lipolytica strains with two different disrupted versions of YlFBP1 and found that they grew much slower than the wild type in gluconeogenic carbon sources but that growth was not abolished as happens in most microorganisms. We attribute this growth to the existence of an alternative phosphatase with a high Km (2.3 mM) for fructose-1,6-bisphosphate. The gene YlFBP1 restored fructose-1,6-bisphosphatase activity and growth in gluconeogenic carbon sources to a Saccharomyces cerevisiae fbp1 mutant, but the introduction of the FBP1 gene from S. cerevisiae in the Ylfbp1 mutant did not produce fructose-1,6-bisphosphatase activity or growth complementation. Subcellular fractionation revealed the presence of fructose-1,6-bisphosphatase both in the cytoplasm and in the nucleus.

Biofilm formation by a biotechnologically important tropical marine yeast isolate, Yarrowia lipolytica NCIM 3589

2008 ◽  
Vol 58 (6) ◽  
pp. 1221-1229 ◽  
Author(s):  
D. H. Dusane ◽  
Y. V. Nancharaiah ◽  
V. P. Venugopalan ◽  
A. R. Kumar ◽  
S. S. Zinjarde
Keyword(s):  
Yarrowia Lipolytica ◽  
Biofilm Formation ◽  
Edible Oils ◽  
Carbon Sources ◽  
Three Dimensional ◽  
Ecological Niches ◽  
Lag Phase ◽  
Biofilm Growth ◽  
Water Media ◽  
Biofilm Development

Biofilm formation by Yarrowia lipolytica, a biotechnologically important fungus in microtitre plates, on glass slide surfaces and in flow cell was investigated. In microtitre plates, there was a short lag phase of adhesion followed by a period of rapid biofilm growth. The fungus formed extensive biofilms on glass slides, whereas in flow-cells a multicellular, three-dimensional microcolony structure was observed. The isolate formed biofilms in seawater and in fresh water media at neutral pH when grown in microtitre plates. The carbon sources differentially affected formation of biofilms in microtitre plates. Lactic acid, erythritol, glycerol, glucose and edible oils supported the formation of biofilms, while alkanes resulted in sub-optimal biofilm development. A variation in the morphology of the fungus was observed with different carbon sources. The results point to the possible existence of highly structured biofilms in varied ecological niches from where the yeast is isolated.

scholarly journals HOG-Independent Osmoprotection by Erythritol in Yeast Yarrowia lipolytica

Genes ◽  
2020 ◽  
Vol 11 (12) ◽  
pp. 1424
Author(s):  
Dorota A. Rzechonek ◽  
Mateusz Szczepańczyk ◽  
Guokun Wang ◽  
Irina Borodina ◽  
Aleksandra M. Mirończuk
Keyword(s):  
Transcription Factor ◽  
Yarrowia Lipolytica ◽  
Carbon Sources ◽  
Hyperosmotic Stress ◽  
Sensitive Strain ◽  
The Other ◽  
High Concentration ◽  
Other Hand ◽  
Yeast Yarrowia Lipolytica ◽  
Better Than

Erythritol is a polyol produced by Yarrowia lipolytica under hyperosmotic stress. In this study, the osmo-sensitive strain Y. lipolytica yl-hog1Δ was subjected to stress, triggered by a high concentration of carbon sources. The strain thrived on 0.75 M erythritol medium, while the same concentrations of glucose and glycerol proved to be lethal. The addition of 0.1 M erythritol to the medium containing 0.75 M glucose or glycerol allowed the growth of yl-hog1Δ. Supplementation with other potential osmolytes such as mannitol or L-proline did not have a similar effect. To examine whether the osmoprotective effect might be related to erythritol accumulation, we deleted two genes involved in erythritol utilization, the transcription factor Euf1 and the enzyme erythritol dehydrogenase Eyd1. The strain eyd1Δ yl hog1Δ, which lacked the erythritol utilization enzyme, reacted to the erythritol supplementation significantly better than yl-hog1Δ. On the other hand, the strain euf1Δ yl-hog1Δ became insensitive to supplementation, and the addition of erythritol could no longer improve the growth of this strain in hyperosmotic conditions. This indicates that Euf1 regulates additional, still unknown genes involved in erythritol metabolism.

Enhanced limonene production by metabolically engineered Yarrowia lipolytica from cheap carbon sources

2022 ◽  
Vol 249 ◽  
pp. 117342
Author(s):  
Shenglong Li ◽  
Lanxin Rong ◽  
Shuhui Wang ◽  
Shiqi Liu ◽  
Zhihui Lu ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Carbon Sources

Metabolomic elucidation of the effects of media and carbon sources on fatty acid production by Yarrowia lipolytica

2018 ◽  
Vol 272-273 ◽  
pp. 7-13 ◽  
Author(s):  
Eun Ju Yun ◽  
James Lee ◽  
Do Hyoung Kim ◽  
Jungyeon Kim ◽  
Sooah Kim ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Yarrowia Lipolytica ◽  
Acid Production ◽  
Carbon Sources ◽  
Fatty Acid Production ◽  
Effects Of Media

The dimorphic yeast Yarrowia lipolytica possesses an atypical phosphofructokinase: characterization of the enzyme and its encoding gene

Microbiology ◽  
2005 ◽  
Vol 151 (5) ◽  
pp. 1465-1474 ◽  
Author(s):  
Carmen-Lisset Flores ◽  
Oscar H. Martínez-Costa ◽  
Valentina Sánchez ◽  
Carlos Gancedo ◽  
Juan J. Aragón
Keyword(s):  
Yarrowia Lipolytica ◽  
Carbon Sources ◽  
Hill Coefficient ◽  
Single Type ◽  
Kinetic Characteristics ◽  
Gene Encoding ◽  
Dimorphic Yeast ◽  
Encoding Gene ◽  
Cooperative Kinetics

The phosphofructokinase from the non-conventional yeast Yarrowia lipolytica (YlPfk) was purified to homogeneity, and its encoding gene isolated. YlPfk is an octamer of 869 kDa composed of a single type of subunit, and shows atypical kinetic characteristics. It did not exhibit cooperative kinetics for fructose 6-phosphate (Hill coefficient, h 1·1; S 0·5 52 μM), it was inhibited moderately by MgATP (K i 3·5 mM), and it was strongly inhibited by phosphoenolpyruvate (K i 61 μM). Fructose 2,6-bisphosphate did not activate the enzyme, and AMP and ADP were also without effect. The gene YlPFK1 has no introns, and encodes a putative protein of 953 aa, with a molecular mass consistent with the subunit size found after purification. Disruption of the gene abolished growth in glucose and Pfk activity, while reintroduction of the gene restored both properties. This indicates that Y. lipolytica has only one gene encoding Pfk, and supports the finding that the enzyme consists of identical subunits. Glucose did not interfere with growth of the Ylpfk1 disruptant in permissive carbon sources. The unusual kinetic characteristics of YlPfk, and the intracellular concentrations of glycolytic intermediates during growth in glucose, suggest that YlPfk may play an important role in the regulation of glucose metabolism in Y. lipolytica, different from the role played by the enzyme in Saccharomyces cerevisiae.

Engineering Yarrowia lipolytica for the selective and high-level production of isocitric acid through manipulation of mitochondrial dicarboxylate–tricarboxylate carriers

2020 ◽  
Author(s):  
Evgeniya Y. Yuzbasheva ◽  
Pasquale Scarcia ◽  
Tigran V. Yuzbashev ◽  
Eugenia Messina ◽  
Iuliia M. Kosikhina ◽  
...  
Keyword(s):  
Yarrowia Lipolytica ◽  
Isocitric Acid ◽  
High Level ◽  
Level Production
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