scholarly journals UJI AKTIVITAS ANTIBAKTERI EKSTRAK BATANG BUAH NAGA (Hylocereus polyrhizus (F.A.C.Weber) Britton & Rose) TERHADAP Staphylococcus aureus

2021 ◽  
Vol 3 (1) ◽  
pp. 42-47
Author(s):  
Monita Leviyanti ◽  
Sukamawati Sukamawati
Keyword(s):  
Staphylococcus Aureus ◽  
Hylocereus Polyrhizus

Batang buah naga merupakan limbah dari tanaman buah naga yang mengandung senyawa flavonoid, saponin dan steroid. Penelitian lebih lanjut terhadap batang buah naga diperlukan agar dapat memaksimalkan pemanfaatannya. Penelitian ini bertujuan untuk menguji aktivitas antibakteri batang buah naga terhadap bakteri Staphylococcus aureus. Ekstraksi sampel batang buah naga menggunakan metode maserasi dengan etanol 70%. Konsentrasi ekstrak buah naga dibuat menjadi 25%, 50%, 75%, dan 100%. Aquadest sebagai kontrol negatif dan cefixime sebagai kontrol positif. Bakteri dibiakan pada media MHA  menggunakan teknik spread plate, cakram yang telah direndam zat uji ditanamkan pada media. Hasil penelitian menunjukan bakteri sensitif terhadap kontrol positif sedangkan pada konsentrasi ekstrak tidak menunjukan adanya zona hambat yang terbentuk pada semua sehingga diketahui bahwa ekstrak batang buah naga tidak memiliki aktivitas antibakteri

Betacyanin inhibited biofilm formation of co-culture of Staphylococcus aureus and Pseudomonas aeruginosa on different polymer surfaces

2020 ◽  
Author(s):  
Yi Yi Yong ◽  
Michelle Wei Kim Ong ◽  
Gary Dykes ◽  
Wee Sim Choo
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Biofilm Formation ◽  
Bacterial Attachment ◽  
Polymer Surfaces ◽  
Inhibiting Activity ◽  
Hydrophobic Polymers ◽  
Crystal Violet Assay ◽  
Hylocereus Polyrhizus ◽  
Scanning Electron

Abstract Staphylococcus aureus and Pseudomonas aeruginosa are bacteria that cause biofilm-associated infections. The aim of this study was to determine the activity of combined betacyanin fractions from Amaranthus dubius (red spinach) and Hylocereus polyrhizus (red pitahaya) against biofilms formed by co-culture of S. aureus and P. aeruginosa on different polymer surfaces. Various formulations containing different concentrations of the betacyanin fractions were investigated for biofilm inhibiting activity on polystyrene surfaces using the crystal violet assay and scanning electron microscopy. A combination of each betacyanin fraction (0.625 mg mL−1) reduced biofilm formation of five S. aureus strains and four P. aeruginosa strains from OD values of 1.24–3.84 and 1.25–3.52 to 0.81–2.63 and 0.80–1.71, respectively. These combined fractions also significantly inhibited dual-species biofilms by 2.30 and reduced 1.0–1.3 log CFU cm−2 bacterial attachment on polymer surfaces such as polyvinyl chloride, polyethylene, polypropylene and silicone rubber. This study demonstrated an increase in biofilm inhibiting activity against biofilms formed by two species using combined fractions than that of using single fractions. Betacyanins found in different plants could collectively be used to potentially decrease the risk of biofilm-associated infections caused by these bacteria on hydrophobic polymers.

scholarly journals Uji efektivitas ekstrak etanol kulit buah naga merah (Hylocereus polyrhizus) terhadap Staphylococcus aureus ATCC 25923 secara in vitro

2019 ◽  
Vol 10 (3) ◽  
Author(s):  
Anak Agung Bintang Astridwiyanti ◽  
Agung Nova Mahendra ◽  
Ni Wayan Sucindra Dewi
Keyword(s):  
Staphylococcus Aureus ◽  
Hylocereus Polyrhizus

Cytochemical Studies of Cell Wall Biosynthesis in Staphylococcus Aureus

1972 ◽  
Vol 30 ◽  
pp. 328-329
Author(s):  
Masaatsu Koike ◽  
Koichi Nakashima ◽  
Kyoko Iida
Keyword(s):  
Staphylococcus Aureus ◽  
Periodate Oxidation ◽  
Early Time ◽  
The Other ◽  
Penicillin G ◽  
Cell Wall Biosynthesis ◽  
Septal Wall ◽  
Peptidoglycan Biosynthesis ◽  
Gram Positive Bacteria ◽  
Cross Linkage

Penicillin exerts the activity to inhibit the peptide cross linkage between each polysaccharide backbone at the final stage of wall-peptidoglycan biosynthesis of bacteria. Morphologically, alterations of the septal wall and mesosome in gram-positive bacteria, which were occurred in early time after treatment with penicillin, have been observed. In this experiment, these alterations were cytochemically investigated by means of silver-methenamine staining after periodate oxidation, which is applied for detection of localization of wall mucopolysaccharide.Staphylococcus aureus strain 209P treated with 100 u/ml of penicillin G was divided into two aliquotes. One was fixed by Kellenberger-Ryter's OSO4 fixative at 30, 60 and 120 min after addition of the antibiotic, dehydrated through alcohol series, and embedded in Epon 812 (Specimen A). The other was fixed by 21 glutaraldehyde, dehydrated through glycolmethacrylate series and embedded in glycolmethacrylate mixture, according to Bernhard's method (Specimen B).

Gold labeling of teichoic acid on adjacent surfaces of staphylococcus aureus

1990 ◽  
Vol 48 (3) ◽  
pp. 578-579
Author(s):  
Margaret Hukee
Keyword(s):  
Staphylococcus Aureus ◽  
Teichoic Acid ◽  
Protein Labeling ◽  
Minimal Amount ◽  
Section Thickness ◽  
Double Labeling ◽  
Parallel Surfaces ◽  
Supporting Membrane

Gold labeling of two antigens (double labeling) is often done on two section surfaces separated by section thickness. Whether labeling is done on both sides of the same section or on two parallel surfaces separated by section thickness (PSSST), comparable results are dependent on an equal number of epitopes being exposed at each surface. We propose a method to study protein labeling within the same field of proteins, by examining two directly adjacent surfaces that were split during sectioning. The number of labeling sites on adjacent surfaces (AS) were compared to sites on PSSST surfaces in individual bacteria.Since each bacteria needed to be recognizable in all three section surfaces, one-hole grids were used for labeling. One-hole grids require a supporting membrane and excessive handling during labeling often ruptures the membrane. To minimize handling, a labeling chamber was designed that is inexpensive, disposable, minimizes contamination, and uses a minimal amount of solution.

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