scholarly journals Ectopic expression of an AP3-like and a PI-like genes from 'Sekkoku' orchid (Dendrobium moniliforme) causes the homeotic conversion of sepals to petals in whorl 1 and the suppression of carpel development in whorl 4 in Arabidopsis flowers

2010 ◽  
Vol 27 (2) ◽  
pp. 183-192 ◽  
Author(s):  
Supatida Sirisawat ◽  
Hiroshi Ezura ◽  
Naoya Fukuda ◽  
Takahiro Kounosu ◽  
Takashi Handa
2007 ◽  
Vol 50 (5) ◽  
pp. 676-689 ◽  
Author(s):  
JinHui Fan ◽  
WenQing Li ◽  
XiuChun Dong ◽  
Wei Guo ◽  
HuaiRui Shu

2019 ◽  
Vol 71 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Yong Zhou ◽  
Lifang Hu ◽  
Lingli Ge ◽  
Guanghua Li ◽  
Peng He ◽  
...  

The floral homeotic C-function MADS gene AGAMOUS (AG) in Arabidopsis plays crucial roles in specifying stamen and carpel identities as well as determining floral meristem. However, there have been only a few studies of floral homeotic C-function genes in cucumber thus far. In the present study, CsMADS24, a putative AG ortholog from cucumber, was isolated and characterized. Sequence analysis and protein sequence alignment revealed that the deduced CsMADS24 protein contained the typical MIKC structure and the N-terminal extension, as well as two highly conserved AG motifs (I and II). Phylogenetic analysis showed that CsMADS24 fell into the clade of core eudicots, while being distant from the AG orthologs of basal eudicots, monocots and gymnosperms. Expression analysis by RT-PCR showed that CsMADS24 was exclusively expressed in female flower buds. In situ hybridization revealed that CsMADS24 expression was only detected in the carpels. Functional analyses indicated that the sepals were partly converted into carpelloid-like structures in 35S::35S::CsMADS24 transgenic plants. In addition, earlier flowering and delayed floral organ abscission during the development of siliques were also observed in transgenic Arabidopsis. Our findings demonstrate that the AG ortholog plays an exclusive role in carpel specification of cucumber, providing a basis for revealing the mechanisms of reproductive development in cucumber.


2020 ◽  
Vol 45 ◽  
pp. 10-18 ◽  
Author(s):  
Stephen Kironji Githeng’u ◽  
Lian Ding ◽  
Kunkun Zhao ◽  
Wenqian Zhao ◽  
Sumei Chen ◽  
...  

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 513c-513 ◽  
Author(s):  
Martin J. Bukovac ◽  
Jerome Hull ◽  
John C. Neilsen ◽  
Michael Schroeder ◽  
Georg Noga

NAA is used extensively for fruit thinning of apples to increase fruit size and to promote return bloom. In some cultivars, even if thinning is achieved, fruit size at harvest may be less than expected based on crop load. CPPU, N-(2-chloro-4-pyridinyl)-N.-phenylurea, has been shown to increase fruit growth in apples, grapes, and kiwi. We evaluated combinations of NAA and CPPU on thinning, fruit growth and return bloom in Redchief `Delicious', `Elstar', and `Gloster'. CPPU was applied at 5 mgμL–1 (based on 0 to 10 mgμL–1 response curve) in combination with 15 mgμL–1 NAA as high-volume sprays at 7 to 10 mm KFD. Yield and fruit size distribution (on total yield) were used as index of response. In `Delicious', CPPU (3-year study) increased % large (70 mm+) fruit, but in the presence of NAA % large fruit was reduced 2 of the 3 years. CPPU did not induce significant thinning. There were no significant effects on color or soluble solids; firmness was increased slightly and seed number reduced. The L/D ratio was increased and uneven lobe and carpel development was common. CPPU had no significant effect on return bloom in presence or absence of NAA, but NAA increased bloom in both the presence and absence of CPPU. With `Elstar' (2-year study) there was no significant thinning with either chemical, but CPPU increased mean fruit size and % large (70–80 mm) fruit over nonthinned, but not significantly greater than NAA alone. There were no significant differences in firmness, color, soluble solids or seed number. NAA + CPPU did not inhibit fruit growth or cause excessive uneven carpel development. Frost damage reduced crop load in `Gloster' where results were similar to `Elstar' except seed number was reduced by the NAA + CPPU combination.


2019 ◽  
Vol 24 (39) ◽  
pp. 4639-4645 ◽  
Author(s):  
Seyed Mostafa Parizadeh ◽  
Reza Jafarzadeh-Esfehani ◽  
Amir Avan ◽  
Maryam Ghandehari ◽  
Fatemeh Goldani ◽  
...  

Gastric cancer (GC) has a high mortality rate with a poor 5-year survival. Helicobacter pylori (H. pylori) is present as part of the normal flora of stomach. It is found in the gastric mucosa of more than half of the world population. This bacterium is involved in developing H. pylori-induced GC due to the regulation of different micro ribonucleic acid (miRNA or miR). miRNAs are small noncoding RNAs and are recognized as prognostic biomarkers for GC that may control gene expression. miRNAs may function as tumor suppressors, or oncogenes. In this review, we evaluated studies that investigated the ectopic expression of miRNAs in the prognosis of H. pylori positive and negative GC.


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