scholarly journals In vitro Antioxidant Property and Acute Toxicity Study of Methanol Extract of Leaves of Zingiber officinale and Curcuma longa

2021 ◽  
Vol 11 (2) ◽  
pp. 42-45
Author(s):  
Osebhahiemen Ibukun ◽  
Eniola Esther Oluwadare
Keyword(s):  
Acute Toxicity ◽  
Methanol Extract ◽  
Curcuma Longa ◽  
Zingiber Officinale ◽  
Antioxidant Property ◽  
Toxicity Study ◽  
Acute Toxicity Study

scholarly journals In-vitro scavenging activity and acute toxicity study of methanol leaves extract and fractions of Lophira lanceolata Tiegh. Ex Keay (Ochnaceae) in rats.

2016 ◽  
Vol 8 (3) ◽  
pp. 411 ◽  
Author(s):  
Jean-Baptiste N. Oussou ◽  
Isaac J. Asiedu-Gyekye ◽  
Adou F. Yapo ◽  
Benoit Banga N'guessan ◽  
Patrick Amoateng ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Fraction ◽  
Toxicity Study ◽  
Total Phenolic ◽  
Scavenging Activity ◽  
Acute Toxicity Study ◽  
Lophira Lanceolata

<p>The present study aims at comparing <em>in-vitro </em>scavenging activities of methanol extract and fractions of <em>Lophira lanceolata</em> leaves, and also to study the oral acute toxicity of the ethyl acetate fraction.</p><p>Petroleum ether, dichloromethane, ethyl acetate, butanol and water fractions were prepared from the methanol extract of the plant. The scavenging activity of DPPH (2, 2-Diphenyl-1-picrylhydrazyl), the total phenolic (TPC) and total flavonoid (TFC) contents of the extract and fractions were determined by spectrophotometrical methods using gallic acid (GA) and quercetin (Qu) as reference antioxidant. The fifty percent inhibitory concentration (IC<sub>50</sub>), fifty percent effective concentration (EC<sub>50</sub>) and the antiradical power (ARP) were determined for all extract and fractions. An acute toxicity study using a single oral dose of 5000 mg/kg of the ethyl acetate fraction of the plant was conducted in female Albino rats following the OECD (420) Guidelines. Blood samples were collected for hematological and biochemical analysis. Histopathological examinations of the heart, kidney and liver were performed.</p><p>The results showed that the ethyl acetate fraction of <em>L</em>.<em> lanceolata</em> had the highest free radical scavenging activity of DPPH (IC<sub>50</sub>=1,43; EC<sub>50</sub>= 0.07 and ARP = 14,28)  and also contain the highest amount of total phenols (14,4±0,02 mg of GA equivalent/g of plant fraction) and total flavonoids (93,3±0,04 mg of Qu equivalent/g of plant fraction) as compared to other fractions and ascorbic acid (IC<sub>50</sub>=5,82; EC<sub>50</sub>= 3.44; ARP=3,44). The hematological parameters and the lipid profile didn’t show any major change compared to the control group. However, a significant increase of aspartate aminotransferase (AST, p&lt;0.001) and alanine aminotransferase (ALT, p&lt;0.001) showed that the ethyl acetate fraction of the methanol extract of <em>L</em>.<em> lanceolata </em>leaves might not totally be safe for consumption, in the conditions of our experiment.</p>

In-vitro and in-vivo evaluation of antidiabetic activity of Withania coagulans extract

2019 ◽  
Vol 09 ◽  
Author(s):  
Tejas Patel ◽  
B.N. Suhagia
Keyword(s):  
Blood Glucose ◽  
Acute Toxicity ◽  
Aqueous Extract ◽  
Pancreatic Beta Cell ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Withania Coagulans ◽  
Study Results

Background: Diabetes mellitus is major issue to public health as its prevalence is rising day by day. Synthetic agents available for the diabetic treatment are expensive or produce undesirable side effect on chronic use and some of them are not suitable during pregnancy. Herbal medicines accepted widely due to side effects and low cost. Objective: The aim of present study was to evaluate the activity of Withania coagulans extract using In-vitro and In-vivo model. Methods: Different three types of Withania coagulans extract were prepared using aqueous (W1), Alcohol (W2) and hydro-alcoholic (50:50) mixture (W3). In-vitro Anti-diabetic activity of the all three extracts evaluated using RINm5F Pancreatic beta cells.Further, n-vivo anti-diabetic evaluation performed by administering 50 mg/kg (p.o) aqueous extract for 7 days in Streptozotocin (STZ)-induced mice. Body weight of the animals was also determined to perform acute toxicity study. Results: The results of in –vitro cell based study indicated that among all three extract, aqueous extract (W1) of Withania coagulans showed potential increase in inulin release. The EC50 of the W1 (249.6 µg/L) which is compared with standard (Glibenclamide) EC50. From the results of In-vitro study, W1 subjected for acute toxicity study and the acute toxicity study results indicated LD50 of 50mg/kg. Diabetic rats treated with W1 extract at oral dose of 50 mg/kg for 7 days showed 34.17% reduction in blood glucose in comparison to untreated diabetic (STZ-induced) rats. Blood glucose levels of Standard treated (Glibenclamide) and control untreated. Conclusion: In conclusion, results of pancreatic beta cell based study showed increase in insulin release by administration of extract. Further aqueous extract (W1) was potentially reduced blood glucose level in STZ induced diabetic mice.

Amphiphilic poly-N-vinylpyrrolidone nanoparticles as carriers for non-steroidal, anti-inflammatory drugs: In vitro cytotoxicity and in vivo acute toxicity study

2017 ◽  
Vol 13 (3) ◽  
pp. 1021-1030 ◽  
Author(s):  
Andrey N. Kuskov ◽  
Pavel P. Kulikov ◽  
Anastasia V. Goryachaya ◽  
Manolis N. Tzatzarakis ◽  
Anca O. Docea ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
In Vitro Cytotoxicity ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Inflammatory Drugs ◽  
Anti Inflammatory

Acute Toxicity Study and the In Vitro Cytotoxicity of a Black Lingzhi Medicinal Mushroom, Amauroderma rugosum (Agaricomycetes), from Malaysia

2017 ◽  
Vol 19 (12) ◽  
pp. 1093-1099 ◽  
Author(s):  
Shin Yee Fung ◽  
Nget Hong Tan ◽  
Boon Hong Kong ◽  
Sook Shien Lee ◽  
Yee Shin Tan ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
In Vitro Cytotoxicity ◽  
Toxicity Study ◽  
Medicinal Mushroom ◽  
Acute Toxicity Study

Mucopenetrating nanoparticles for enhancement of oral bioavailability of furosemide: In vitro and in vivo evaluation/sub-acute toxicity study

2017 ◽  
Vol 526 (1-2) ◽  
pp. 366-379 ◽  
Author(s):  
Salma El-Sayed Radwan ◽  
Magda Samir Sokar ◽  
Doaa Ali Abdelmonsif ◽  
Amal Hassan El-Kamel
Keyword(s):  
Acute Toxicity ◽  
Oral Bioavailability ◽  
Toxicity Study ◽  
In Vivo Evaluation ◽  
Acute Toxicity Study

scholarly journals In-Vitro Antioxidant, In-Vivo Anti-Inflammatory, and Acute Toxicity Study of Indonesian Propolis Capsule from Tetragonula sapiens

2021 ◽  
Author(s):  
Siti Farida ◽  
Diah Kartika Pratami ◽  
Muhamad Sahlan ◽  
Dian Ratih Laksmitawati ◽  
Etin Rohmatin ◽  
...  
Keyword(s):  
Acute Toxicity ◽  
Toxicity Study ◽  
Acute Toxicity Study ◽  
Anti Inflammatory

PHARMACOGNOSTIC, ANTIOXIDANT AND ACUTE TOXICITY STUDY OF Ficus sycomorus (Linn) (Moraceae) ROOT AND STEM BARK

2020 ◽  
Vol 4 (2) ◽  
pp. 605-614
Author(s):  
Murtala M. Namadina ◽  
H. Haruna ◽  
U. Sanusi
Keyword(s):  
Acute Toxicity ◽  
Radical Scavenging ◽  
Stem Bark ◽  
The Body ◽  
Toxicity Study ◽  
Bark Extract ◽  
Root Extract ◽  
Acute Toxicity Study ◽  
Radical Scavenging Ability ◽  
Phytochemical Components

Most of biochemical reactions in the body generates Reactive Oxygen Species (ROS), which are involved in the pathogenesis of oxidative stress-related disorders like diabetes, nephrotoxicity, cancer, cardiovascular disorders, inflammation and neurological disorders when they attack biochemical molecules like proteins, lipids and nucleic acid. Antioxidants are used to protect the cells or tissues against potential attack by ROS. Most medicinal plants possess a rich source of antioxidants such as flavonoids, phenols, tannins, alkaloids among others. These phytochemicals are currently pursued as an alternative and complimentary drug. In this study, phytochemical components, antioxidant and acute toxicity study of the methanol extract of stem bark and root of F. sycomorus were carried out using standard methods. Findings from this study revealed the presence of some diagnostic microscopical features such as calcium oxalate, starch, gum/mucilage, lignin, Aleurone grain, suberized/Cuticular cell wall and inulin but calcium carbonate was absent in stem bark but present in the powdered root. Quantitative physical constants include moisture contents (6.40% and 7.82%), ash value (7.20% and 9.30 %) in stem bark and root respectively. Carbohydrates, alkaloid, flavonoids, saponins, tannins, glycoside, steroid, triterpenes and phenols were present in all the extracts. They were found to exhibit potent 1,1,-diphenyl 2-picryl hydrazyl (DPPH) free scavenging activity. The DPPH radical scavenging ability of the extracts showed the following trend Ascorbic acid < stem bark extract˃ root extract. The LD50 of the methanolic stem bark and root extracts were found to be greater than 5000 mg /kg and is considered safe for use. Nonetheless, further

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