Lophira lanceolata protects testicular and spermatological damages induced by cisplatin in male Wistar rats

Abstracts Background Chemotherapy is associated with male infertility. Cisplatin (CP), an antineoplastic agent has been successfully used for the treatment of diverse kinds of malignancies, however, the use of this effective agent could induce oxidative stress injury, nephrotoxicity, hepatotoxicity, and testicular damage. Combined CP chemotherapy with plant extracts can diminish the toxicity and enhance the antitumor efficacy of the drug. The objective of the study was to determine the protective effect Lophira lanceolata leaf extract (LLLE) on CP-induced toxicity on male reproductive organs. Methods The study was carried out with 30 (n = 30) male Wistar rats (Rattus norvegicus). The rats were randomly assigned into 6 groups of 5 rats each. Rats in group 1 (Control) were administered distilled water per os. Rats in group 2 were administered 5 mg/kg of CP intraperitoneally (i.p). Rats in groups 3 and 4 were administered per os LLLE at the doses of 200 and 400 mg/kg body weight and rats in groups 5 and 6 were administered 5 mg/kg body weight of CP + LLLE at the doses of 200 and 400 mg/kg body weight respectively. Results The results showed a significant decrease in the sperm parameters in the group treated with CP alone when compared with the control and there in the sperm parameters in the groups administered CP + LLLE. The body and organ weights of the rats were significantly (p < 0.05) decreased in the CP treated group relative to the control. However, there was an increase in the weight of the organs in the LLLE pretreated groups. The photomicrographs showed degenerative changes in the testicular tissues of the rats administered CP alone whereas the group pretreated with the LLLE showed amelioration induced by the CP. Our study revealed that CP treatment has deleterious effects on sperm parameters and testicular tissues and the accessory sex organs (Epididymis, prostate, seminal vesicles) of the rats. Oral administration of LLLE at 200 and 400 mg/kg bodyweight for 26 days conferred protective effects against testicular damage induced by CP. Conclusion This study revealed that pretreatment with LLLE protected against CP-induced testicular toxicity.

Etude dendrométrique de Lophira lanceolata de la forêt classée de Toui-Kilibo et ses environs au Bénin

La connaissance des paramètres dendrométriques d’une espèce est nécessaire pour sa conservation durable. Elle permet d’évaluer les ressources disponibles et de lutter contre sa destruction. Cette étude analyse les paramètres dendrométriques de Lophira lanceolata de la forêt classée de Toui-Kilibo et ses environs au Bénin. Les données dendrométriques sont collectées à l’intérieur de 64 placettes carrées de 30 mètres de côté. L’analyse des données prend en compte la contribution en surface terrière de Lophira lanceolata, la densité relative, la dominance relative, la fréquence, l’amplitude d’habitat, le diamètre moyen par les types de formations végétales. Le diamètre moyen de Lophira lanceolata (25,5 cm), la hauteur moyenne (15,21 cm) et la surface terrière moyenne (0,63 m2/ha) de la forêt claire et savane boisée sont supérieurs aux autres types de formations végétales. Ce constat est fait également sur la fréquence, la densité relative, la dominance relative. La contribution en surface terrière de Lophira lanceolata est de 9,363% en forêt claire et savane boisée, 7,398% en savane arborée et arbustive et 2,65% en champs et jachères. En forêt claire et savane boisée, lorsque le diamètre de Lophira lanceolata s’accroît d’un centimètre, sa surface terrière augmente de 0,043 m2/ha. L’étude permet de connaître les ressources disponibles sur Lophira lanceolata et de prendre les décisions sur son aménagement.Mots clés : Lophira lanceolata, paramètres dendrométriques, forêt, savane, Bénin. English Title: Dendrometric study of Lophira lanceolata of the Toui-Kilibo classified forest and its surroundings in Benin Knowledge of the dendrometric parameters of a species is necessary for its sustainable conservation. It makes it possible to assess the resources available and to combat its destruction. This study analyses the dendrometric parameters of Lophira lanceolata of the classified forest of Toui-Kilibo and its surroundings in Benin. Dendrometric data is collected inside 64 square plots 30 meters wide. Data analysis concerns the contribution in basal area of Lophira lanceolata, the relative density, the relative dominance, the frequency, the range of habitat, the average diameter by the types of plant formations. The average diameter of Lophira lanceolata (25.5 cm), the average height (15.21 cm) and the average basal area (0.63 m2/ha) of the open forest and wooded savannah are superior to other types of plant formations. This observation is also made on the frequency, the relative density, the relative dominance. The contribution in basal area of Lophira lanceolata is 9.363% in open forest and wooded savannah, 7.398% in tree and shrubby savannah and 2.65% in fields and fallows. In open forest and wooded savannah, when the diameter of Lophira lanceolata increases by one centimeter its basal area increases by 0.043 m2/ha. The study makes it possible to know the resources available on Lophira lanceolata and to take decisions on its development.Keywords: Lophira lanceolata, dendrometric parameters, forest, savanna, Benin.

Protective effect of aqueous extract of Lophira lanceolata leaf against cisplatin-induced hepatorenal injuries and dyslipidemia in Wistar rats

Background Hepatorenal injuries and dyslipidemia are common global health challenges but medicinal plant extracts may have potential to prevent them. Thus, this study evaluated the protective effect of aqueous extract of Lophira lanceolata leaf (LLE) against cisplatin-induced hepatorenal injuries and dyslipidemia in albino Wistar rats. Methods Thirty rats were randomly divided into 6 groups of 5 rats each. Group I rats received distilled water and served as control, group II rats were given 5 mg/kg cisplatin (CIS) intraperitoneally, groups III and IV rats were treated with 200 and 400 mg/kg LLE respectively for 26 days by oral gavages while groups V and VI rats were treated with 200 and 400 mg/kg LLE respectively, followed by CIS on the 21st day as in group II. About 24 h after treatment, blood was collected from the rats; then serum was separated and used for estimations of biochemical parameters. The kidney and liver of rats were removed, rinsed in normal saline, stored in 10% formalin and used for histological analyses. Results The biomarkers of hepatic (Aminotransferases, Alkaline phosphatase and Bilirubin) and renal (urea and creatinine) injuries, and dyslipidemia (Total cholesterol, triglycerides and LDL-cholesterol) significantly (p < 0.05) increased in the rats exclusively exposed to cisplatin when compared with normal control. However, treatment of cisplatin-exposed rats with 200 and 400 mg/kg LLE significantly (p < 0.05) reduced the levels of these biomarkers of hepatorenal injuries and dyslipidemia when compared with cisplatin control. Photomicrographs showed pathological signs in the liver and kidney of rats exclusively exposed to cisplatin, but there was moderate protection of these tissues in the rats treated with LLE and cisplatin. Conclusion The current findings have shown that Lophira lanceolata leaf extract may provide moderate protection against cisplatin-induced hepatorenal injuries and dyslipidemia in albino Wistar rats.