scholarly journals Total Phenolic and Flavonoid Contents, Antioxidant and Antimicrobial Activities of Traditional Unripe Grape Products

2017 ◽  
Vol 51 (3s2) ◽  
pp. s489-s493 ◽  
Author(s):  
Filiz Ucan Turkmen ◽  
Hatice Aysun Mercimek Takci ◽  
Nazım Sekeroglu
2021 ◽  
Vol 45 (2) ◽  
pp. 223-231
Author(s):  
Kara Guzel ◽  
Mahmut Ulger ◽  
Ahmet Kahraman

The phytochemical contents of Salvia virgata mericarps and the antioxidant and antimicrobial activities of its ethanol extract were studied for the first time. S. virgata mericarps were collected in the province of Trabzon, Turkey. Chemical analyses were performed using chromatographic methods. The total phenolic and flavonoid contents and antioxidant potential were measured using Folin-Ciocalteu, Al(NO3)3, and DPPH radical scavenging activity methods, respectively. The antimicrobial activity was evaluated using the microdilution method for all the tested bacterial and fungal strains, with the exception of Mycobacterium tuberculosis for which the resazurin microtiter plate method was applied. The mericarps were rich in glutamic acid (3934 mg/100 g), potassium (12578.8 ?g/g), calcium (12092.0 ?g/g), and dietary fibers (35.565 g/100 g). The total phenolic and flavonoid contents were 2.50 ?g GAE/mg extract and 0.34 ?g QE/mg extract, respectively. The most effective DPPH free radical scavenging activity determined for the highest applied concentration was 92.44%. The ethanol extract obtained from the mericarps was found to be as effective as the reference drug ampicillin (MIC value = 125 ?g/mL) against the nosocomial pathogen Acinetobacter baumannii. In conclusion, S. virgata mericarps provide good nutritional value with low amounts of carbohydrates and high dietary fibers, amino acids, minerals and total phenolic and flavonoid contents and medicinal properties.


Plants ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 149
Author(s):  
Fatima Zahra Jawhari ◽  
Abdelfattah E. L. Moussaoui ◽  
Mohammed Bourhia ◽  
Hamada Imtara ◽  
Hamza Saghrouchni ◽  
...  

In this work, two varieties of Anacyclus pyrethrum (L.) including Anacyclus pyrethrum var. pyrethrum (L.) and Anacyclus pyrethrum var. depressus (Ball) Maire were evaluated for their mineral and chemical compositions, total phenolic and flavonoid contents, and antimicrobial and antioxidant activities using hydroalcoholic extracts from their different parts (leaves, capitula, roots, and seeds). The phytochemical and mineral compositions were carried out using standard methods. The antioxidant activity was determined using the DPPH (2,2-diphenyl-1-picrylhydrazyl), ABTS (2,2-azino-bis 3-ethylbenzothiazolin-6-sulfonic acid), and FRAP (ferric reducing antioxidant power) tests. The antimicrobial activity was assayed using the agar diffusion, minimum inhibitory concentration, and minimum bactericidal concentration methods. The results of the chemical analysis showed that both varieties contained interesting mineral and chemical compositions with potentially active compounds; among them, N-isobutyl-2,4-heptadiene-6-monoynamide and cinnamic acid were detected in the Anacyclus pyrethrum var. pyrethrum (L.) only while thiadiazolo [5,4-d] pyrimidin-7-amine and N-isobutyl-2,4-undecadiene-8,10-diynamide compounds were limited to the Anacyclus pyrethrum var. depressus (Ball) Maire. In vitro antioxidant and antimicrobial activities of the two varieties demonstrated that the different parts had prominent antioxidant and antimicrobial properties. The principal component analysis (PCA) showed great similarity in the activity of the leaves, capitula, and seeds of both plants and a high difference in roots. Anacyclus pyrethrum var. pyrethrum roots were characterized by a high content in phenols and flavonoids and better antibacterial activities compared to Anacyclus pyrethrum var. depressus (Ball) Maire roots, which were characterized by better antioxidant activities. From this study, it can be concluded that the two varieties of Anacyclus pyrethrum (L.) showed promising mineral and chemical compositions with antioxidant and antimicrobial properties.


Author(s):  
Nazan Çömlekcioğlu ◽  
Ashabil Aygan

Hibiscus sabdariffa L. (Hibiskus) is a plant that belongs to the Malvaceae family and and is a rich source of anthocyanins and other bioactive compounds. In this study, the total phenolic and flavonoid content, antioxidant and antimicrobial activities of commercially supplied Hibiscus sabdariffa calyx extracts obtained by two different extractors (soxhlete and ultrasonic bath) were investigated. In addition, fatty acids were analysed by GC-MS analysis, 14 different fatty acids were determined and the major fatty acid components of extracts obtained from plant calyxes were palmitic acid (15.25%) and oleic acid (32.18%) and linoleic acid (30.77%). The ultrasonic bath was found to be more effective to reveal the bioactive contents of the extracts than the soxhlete. Total phenolic content and flavonoid values of plant calyx extracts (USB) were 23.29 and 3.08 mg ml-1, respectively, and FRAP and IC50 (% DPPH) values were 47.54 µg g-1 and 0.61 mg ml-1, respectively. Antimicrobial activity experiment carried out with total of 9 microorganisms consisting of seven bacteria and two yeast showed that H. sabdariffa extracts had dose-depended inhibition on test microorganisms but S. cerevisia.


Molecules ◽  
2021 ◽  
Vol 26 (9) ◽  
pp. 2746
Author(s):  
Giuseppe Squillaci ◽  
Carla Zannella ◽  
Virginia Carbone ◽  
Paola Minasi ◽  
Veronica Folliero ◽  
...  

The purpose of the current study was to determine the phenolic composition, antioxidant, and antimicrobial activities in grape cane extracts from typical cultivars of Southern Italy. Aqueous extracts at different pHs (1–13) were prepared from “Aglianico”, “Fiano”, and “Greco” grape canes. The results demonstrated that an alkaline pH (13.00) produced the best polyphenol-rich extracts, as the total phenolic content was more than double when compared to the respective extracts prepared at pH 1.00. “Greco” grape canes gave the highest quantity of phenolic compounds at each pH, ranging from 42.7 ± 0.4 to 104.3 ± 3.0 mg Gallic Acid Equivalents (GAE)/g Dry Extract (DE) from pH 1.00 to 13.00. The Radical Scavenging Activity (RSA) and the Ferric Reducing Antioxidant Power (FRAP) were measured. The highest antioxidant activity was showed by “Greco” extract at pH 7.00. Seventy-five compounds were identified in the extracts by HPLC-MS with six of them described for the first time in grape canes. Procyanidins were highly abundant in extracts at pH 7.00, whereas stilbenoids were the most represented compounds at pH 13.00. Very strong antiviral activity against herpes simplex viruses was recorded for the extracts at pH 7.00 and 13.00 that were active in the early stages of infection by acting directly against the viral particles. The overall results suggest that grape canes, currently underutilized, can be usefully valorised by providing active extracts to use as antioxidant and antiviral agents.


2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Alper Gökbulut ◽  
Onural Özhana ◽  
Basri Satılmiş ◽  
Kadir Batçioğlu ◽  
Selami Günal ◽  
...  

Three Inula species, I. viscosa, I. helenium ssp. turcoracemosa and I. montbretiana, collected from different locations of Anatolia were investigated for their antioxidant and antimicrobial potential, and their total phenolic content and phenolic composition. Antioxidant activities of various extracts of the plant parts were measured using DPPH radical scavenging and ABTS assays. Antimicrobial potential of methanol extracts of the plant parts was determined by the agar dilution method against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Candida tropicalis. All the extracts were more active against Gram-positive bacteria and yeasts than Gram-negative bacteria. The extracts exhibited antioxidant and antimicrobial activities in different concentrations. Total phenolic concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard. The total phenolic content varied widely in different parts of the three tested Inula species, ranging from 21.1 ± 0.8 to 190.9 ± 6.1 mg GAE/g extract. Phenolic components, such as chlorogenic acid, caffeic acid, rutin, myricetin, quercetin, luteolin and kaempferol were quantified by HPLC-DAD in the methanol extracts of the Inula species. It was obvious that the antioxidant and antimicrobial properties of the plants were due to the phenolics.


2008 ◽  
Vol 73 (6) ◽  
pp. 597-607 ◽  
Author(s):  
Sasa Stojicevic ◽  
Ivana Stanisavljevic ◽  
Dragan Velickovic ◽  
Vlada Veljkovic ◽  
Miodrag Lazic

This paper presents a comparative study of the anti-oxidant and anti-microbial activities, total phenolic compounds and total flavonoids in extracts obtained from houseleek (Sempervivum marmoreum L.) leaves by the classical (maceration), ultrasonic and Soxhlet extraction (CE, UE and SE, respectively). The extract obtained by the CE contained higher amounts of phenolic and flavonoid compounds and showed a better antioxidant activity than those obtained using other two techniques. All the extracts, independent of the extraction technique applied, showed antimicrobial activities against Aspergillus niger and Candida albicans only but not against the tested bacteria.


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