Total phenolic content, antiradical, antioxidant and antimicrobial activities of Rhododendron honeys

2010 ◽  
Vol 121 (1) ◽  
pp. 238-243 ◽  
Author(s):  
Sibel Silici ◽  
Osman Sagdic ◽  
Lutfiye Ekici
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Antioxidant And Antimicrobial Activities

scholarly journals Antioxidant and Antimicrobial Activities, and Phenolic Compounds of Selected Inula species from Turkey

2013 ◽  
Vol 8 (4) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Alper Gökbulut ◽  
Onural Özhana ◽  
Basri Satılmiş ◽  
Kadir Batçioğlu ◽  
Selami Günal ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Methanol Extracts ◽  
Plant Parts ◽  
Antimicrobial Potential ◽  
Phenolic Components ◽  
Antioxidant And Antimicrobial Activities

Three Inula species, I. viscosa, I. helenium ssp. turcoracemosa and I. montbretiana, collected from different locations of Anatolia were investigated for their antioxidant and antimicrobial potential, and their total phenolic content and phenolic composition. Antioxidant activities of various extracts of the plant parts were measured using DPPH radical scavenging and ABTS assays. Antimicrobial potential of methanol extracts of the plant parts was determined by the agar dilution method against Staphylococcus aureus, Enterococcus faecalis, Escherichia coli, Pseudomonas aeruginosa, Candida albicans and Candida tropicalis. All the extracts were more active against Gram-positive bacteria and yeasts than Gram-negative bacteria. The extracts exhibited antioxidant and antimicrobial activities in different concentrations. Total phenolic concentration of the extracts was estimated with Folin-Ciocalteu reagent using gallic acid as standard. The total phenolic content varied widely in different parts of the three tested Inula species, ranging from 21.1 ± 0.8 to 190.9 ± 6.1 mg GAE/g extract. Phenolic components, such as chlorogenic acid, caffeic acid, rutin, myricetin, quercetin, luteolin and kaempferol were quantified by HPLC-DAD in the methanol extracts of the Inula species. It was obvious that the antioxidant and antimicrobial properties of the plants were due to the phenolics.

scholarly journals Determination of total phenol, antioxidant and antimicrobial activities of Avena sativa and Ocimum basilicum

2014 ◽  
Vol 11 (2) ◽  
pp. 1062-1066
Author(s):  
Baghdad Science Journal
Keyword(s):  
Antimicrobial Activity ◽  
Antioxidant Capacity ◽  
Avena Sativa ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antimicrobial Activities ◽  
Ocimum Basilicum ◽  
Total Phenolic ◽  
Antioxidant And Antimicrobial Activities ◽  
Plants Extract

The present study investigated the total content of phenolic compounds, antioxidant and antimicrobial activities of water extracts oat (Avena sativa) and basil (Ocimum basilicum), medicinal plants. The Folin-ciocalteu reagent assay was used to estimate the total phenolic content of plants extract. The antioxidant capacity of the plants extract was tested by ferric reducing/antioxidant power Assay (FRAP) and ferric reducing scavenging activity using DPPH method, and the antimicrobial activity was measured against [Staphylococcus epidermidis; Staphylococcus aureus; Proteus spp.; Klebsiella spp.; Escherichia coli; Candida albicans] as tester strains. The total phenolic content of Avena sativa and Ocimum basilicum extracts revealed that the mixture of plants showed higher content. The mixture of Avena sativa and Ocimum basilicum extracts showed the highest antioxidant capacity followed by Avena sativa extract and Ocimum basilicum extract with FRAP and DPPH assay. However, mixture of Avena sativa and Ocimum basilicum extracts exhibited the highest antimicrobial activity when compared to the other extracts. Thus the study revealed that the consumption of mixture herbs may enhance the immune power of our body against diseases due to free radicals.

Total Phenolic Content, Total Flavonoid Content, Antioxidant and Antimicrobial Activities of Malaysian Shorea

2012 ◽  
Vol 6 (7) ◽  
pp. 489-499 ◽  
Author(s):  
Norizan Ahmat ◽  
Norhazana Norizan ◽  
Sharifah Aminah Syed Mohamad ◽  
Nur Ainaa Atikah Moh Nazri ◽  
Siti Saidatul Akmar Ramli ◽  
...  
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antimicrobial Activities ◽  
Total Flavonoid ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content ◽  
Antioxidant And Antimicrobial Activities

scholarly journals Antifungal and Antibacterial Activities of Apple Vinegar of Different Cultivars

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Driss Ousaaid ◽  
Hassan Laaroussi ◽  
Meryem Bakour ◽  
Hayat Ennaji ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Antimicrobial Effect ◽  
Antibacterial Activities ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Plate Method ◽  
Golden Delicious ◽  
Pathogenic Microbes

This study was designed to assess the antimicrobial potencies of apple vinegar against pathogenic microbes. The acidity and total phenolic content were carried out by titration with NaOH 0.1 N and the Folin–Ciocalteu method, respectively, while the spread plate method, agar well diffusion, and MIC assays were used to determine the antimicrobial activities of different vinegar samples. Acidity and phenolic content were dependent on the variety, where the highest values were observed in S2 with 4.02 ± 0.04% and 1.98 ± 0.05 mg GAE/mL for acidity and total phenolic content, respectively. The spread plate method revealed that samples S1 and S2 obtained from the Red delicious variety and Golden delicious variety, respectively, inhibit the growth of all tested strains, while S3 obtained from different varieties and S4 obtained from the Gala royal variety inhibit only two microbes (Escherichia coli and Vibrio cholerae). Sample S1 presented moderate antimicrobial effect against all examined strains with a diameter of inhibition ranging from 11 ± 0.7 to 19 ± 0.5 mm and with MIC values ranging between 1/2 and 1/100. The findings of the current study confirm the usefulness of apple vinegar as a natural sanitizer that inhibits the growth of pathogenic microbes.

scholarly journals Phytochemical screening and antimicrobial activity of stem extract of Coleus forskohlii L. collected from Al-Baha Area, Saudi Arabia

2021 ◽  
Vol 16 (2) ◽  
pp. 078-086
Author(s):  
Abdulaziz Yahya Al-Ghamdi
Keyword(s):  
Ethyl Acetate ◽  
Petroleum Ether ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Petroleum Ether Extract ◽  
Ethanol Extract ◽  
Antimicrobial Activities ◽  
Chloroform Extract ◽  
Total Phenolic ◽  
Phytochemical Screening

This study aimed to investigate the phytochemical screening, total phenolic content, antioxidant and antimicrobial activities of Coleus forskohlii L. stem extract in Al-Baha area, Saudi Arabia. Stem samples were collected from Al-Baha area and air-dried followed by extraction with ethanol, petroleum ether, chloroform, ethyl acetate, and n-butanol. The extracts were then subjected to phytochemical screening, determination of total phenolic content, antioxidant and antimicrobial activities. Results showed the presence of flavonoids, alkaloids, tannins, terpenoids, steroids, saponins, and reducing sugars. Total phenolic content was significantly (P<0.001) higher in n-butanol extract (274.33±3.29 mg GAE/gm), followed by ethyl acetate extract (182.94±1.82 mg GAE/gm), ethanol extract (79.63±2.02 mg GAE/gm) and petroleum ether extract (73.38±3.07 mg GAE/gm), while the lowest content was in chloroform extract (60.06±2.12 mg GAE/gm). The antioxidant activity was significantly (P<0.001) higher in n-butanol extract (67.68±1.55%), followed by ethyl acetate extract (43.38±1.27%), ethanol extract (36.02±1.29%), petroleum ether extract (20.71±0.59%) and chloroform extract (19.73±0.74%). The antimicrobial activity showed that all microorganisms tested were resistant at the concentration of 25 and 50 mg/ml of plant extracts, whereas the concentrations of 100, 150 and 200 mg/ml showed varying activities against gram-negative (Escherichia coli, Pseudomonas aeruginosa), gram-positive (Staphylococcus aureus, Bacillus cereus) and Candida albicans. The study concluded that the stem extracts of C. forskohlli have promising pharmacological and biological activities that could be beneficial in pharmaceutical as well as food and medicinal industries.

scholarly journals In-Vitro Studies on Antitumour and Antimicrobial Activities of Methanolic Kernel Extract of Mangifera Indica L. Cultivar Banganapalli

2019 ◽  
Vol 12 (1) ◽  
pp. 357-362 ◽  
Author(s):  
N. Venkata Raju ◽  
Karuganti Sukumar ◽  
G. Babul Reddy ◽  
P. K. Pankaj ◽  
Muralitharan G. ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Mangifera Indica ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Total Flavonoid Content ◽  
Flavonoid Content

Mangifera indica L. belongs to the family Anacardiaceae and is considered as “King of all Fruits”. Mango kernels are discarded as waste after the industrial processing and it has several proven medicinal benefits. Attempts were made to study its antitumour and antimicrobial activities. In the current research work, 15 local cultivars of Mangifera indica L. were collected with a motto to screen the best cultivar having high total phenolic content, flavonoid content, antitumour property and antimicrobial activity. Banganapalli cultivar of mango showed high total phenolic content and total flavonoid content i.e. 63.5±1.1 mg GAE/g and 16.7±0.5 mg quercetin/g followed by Royal special cultivar (TPC-58.7±0.6 mg GAE/g TFC-16.2±0.6 mg quercetin/g). Mangifera indica L. cultivar Banganapalli which showed highest total phenolic content and total flavonoid content was screened for its antitumour and antimicrobial properties. Antitumour property was tested by using potato disc assay which recorded 40.12% tumour inhibition percentage. Antimicrobial activity was assessed by agar diffusion method by taking 3 test microorganisms viz. Bacillus subtilis subsp. subtilis DSM 10, Staphylococcus aureus MTCC 737 and Escherichia coli MTCC 46. The measured area of inhibition is around Staphylococcus aureus MTCC 737 in 8.5±0.3 mm followed by E.coli MTCC 46 (8.2±0.3 mm) and Bacillus subtilis sub subtilis (6.6±0.5 mm). The present study showed that the mango kernels which were generally discarded as waste has antitumour and antibacterial properties and further studies need to be carried out.

Total Phenolic Content, Antioxidant and Antibacterial Properties of Scurrula ferruginea Extracts

2014 ◽  
Vol 70 (5) ◽  
Author(s):  
Mohsen Marvibaigi ◽  
Neda Amini ◽  
Eko Supriyanto ◽  
Shajarahtunnur Jamil ◽  
Fadzilah Adibah Abdul Majid ◽  
...  
Keyword(s):  
Antioxidant Capacity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Antibacterial Properties ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Antioxidant Compounds ◽  
Dependent Manner ◽  
Stem Extract

Discovery of new therapeutic agents from nature, especially plants is one of the promising approaches for treatment of various diseases. In traditional medicine Scurrula ferruginea is applied to treat some disorders. To the best of our knowledge, there are no investigations on antioxidant capacity and antimicrobial activities of S.ferruginea in Malaysia. The present study was conducted to determine total phenolic content, Fe2+ chelating activity, antioxidative and antimicrobial potential of flowers, leaves and stems of S.ferruginea extracts. Antioxidant capacity, and total phenolic content of extracts were evaluated using DPPH free radical scavenging and Folin-Ciocalteu assays. Antibacterial properties were evaluated by disc diffusion, minimum inhibitory concentration and minimum bactericidal concentration methods. Results indicated the highest total phenolic content for stem extract (309.069). All S. ferruginea extracts exhibited antioxidant activity in a dose dependent manner. Stem extract showed capacity to scavenge free radicals and it was also found to chelate Fe2+ better than others. All extracts presented moderate inhibition ability against selected bacteria. The most significant values of MIC and MBC were belonged to the stem extract. These findings suggest that acetone extracts of S. ferruginea, particularly stem extract, are potentially sources of antioxidant compounds.

scholarly journals Antioxidant and Antibacterial Activity of Methanol Extract of Momordica Balsamina

2018 ◽  
Vol 10 (2) ◽  
pp. 7
Author(s):  
Sajini Souda ◽  
Saramma George ◽  
Naledi Mannathoko ◽  
Irene Goercke ◽  
Kelvin Chabaesele
Keyword(s):  
Total Phenolic Content ◽  
Methanol Extract ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Total Phenolic ◽  
Total Antioxidant ◽  
Zones Of Inhibition ◽  
Gram Negative Organisms

<em>Momordica balsamina </em>is considered as a miracle herb used in African traditional medicine due to its tremendous medicinal and nutritional properties. In this present study we evaluated the antioxidant and antimicrobial activities of the methanol extract of <em>Momordica balsamina </em>(MEMB). Phytochemical screening followed by thin layer chromatography and spectrophotometrydone to measure the radical scavenging activity of 2,2-diphenyl-l-picrylhydrazyl (DPPH). Total phenolic content and total antioxidant activity of MEMB was determined. Zones of inhibition, minimum inhibitory (MIC) and minimum bactericidal concentrations (MBC) were measured by agar diffusion and liquid broth dilution assays to assess the antimicrobial activities of MEMB. Phytochemicals isolated were flavonoids, tannins, coumarins, terpenoids and phenols. The antioxidant potential and antimicrobial activity of MEMB can be attributed to the total phenolic content and other bioactive phytochemicals.MEMB showed antimicrobicidal activity more against Gram positive than Gram negative organisms. <em>S.agalactiae,S.aureus</em> (ATCC 25923) and <em>L.monocytogenes</em> were more susceptible than <em>Proteus mirabilis</em>, <em>E.coli, K.pneumoniae, P.aeruginosa or S. typhimurium. </em>MEMB also showed antifungal activity against <em>C.albicans</em> species though at a higher concentration[MIC - 0.938mg/ml and MBC-1.875mg/ml]. MEMB can therefore be considered as a potential medication in the management of infectious diseases.

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