Analyzing of Urine 3-Hidroxy Propil Mercapturic Acid on Cyclophosphamide Induced Rat to Determine Ameliorating Effect of Leucaena leucocephala (Lam.) de Wit Seed Extract

Abstract. Staphylococcus aureus merupakan flora normal kulit manusia yang bisa menyebabkan infeksi ketika sistem imun tubuh manusia sedang lemah. Pengobatan Staphylococcus aureus tergolong sulit karena Staphylococcus aureus mudah resisten terhadap beberapa antibiotik sehingga dikenal istilah Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-intermediate Staphylococcus aureus (VISA), dan Vancomycin-resistant Staphylococcus aureus (VRSA) yang menunjukkan resistensi tersebut. Pertumbuhan Staphylococcus aureus dapat dihambat ekstrak daun petai cina (Leucaena leucocephala). Tanaman petai cina (Leucaena leucocephala) digunakan masyarakat Indonesia sebagai obat tradisional untuk menyembuhkan luka, mengobati cacingan, dan mengobati jerawat. Tujuan penelitian ini adalah untuk mengetahui aktivitas antibakteri ekstrak biji petai cina (Leucaena leucocephala) dalam menghambat pertumbuhan Staphylococcus aureus.Penelitian ini merupakan eksperimental laboratorium dengan metode dilusi sebanyak 6 replikasi. Setiap replikasi menggunakan konsentrasi ekstrak biji petai cina (Leucaena leucocephala) sebesar 3,125%, 6,25%, 12,5%, 25%, 50%, dan 100% untuk mencari konsentrasi hambat minimal dan konsentrasi bunuh minimal terhadap Staphylococcus aureus.Hasil penelitian tidak dapat menentukan konsentrasi hambat minimal (KHM) ekstrak biji petai cina (Leucaena leucocephala) terhadap Staphylococcus aureus karena warna ekstrak yang gelap. Sedangkan konsentrasi bunuh minimal (KBM) ekstrak biji petai cina (Leucaena leucocephala) terhadap Staphylococcus aureus ditemukan pada konsentrasi 50%, yang menunjukkan bahwa konsentrasi 50% adalah konsentrasi terkecil dimana tidak terdapat pertumbuhan bakteri Staphylococcus aureus.Sebagai simpulan, ekstrak biji petai cina (Leucaena leucocephala) memiliki aktivitas antibakteri terhadap Staphylococcus aureus. Kata kunci: Staphylococcus aureus, Leucaena leucocephala, metode dilusi Abstract. Staphylococcus aureus is a normal human skin flora that can cause infection when human immune system is weak. Staphylococcus aureus treatment is difficult because Staphylococcus aureus is easily resistant to some antibiotics so several terms such as Methicillin-resistant Staphylococcus aureus (MRSA), Vancomycin-intermediate Staphylococcus aureus (VISA), and Vancomycin-resistant Staphylococcus aureus (VRSA) are known to indicate the resistance. The growth of Staphylococcus aureus can be inhibited by Leucaena leucocephala leaf extract. Leucaena leucocephala is used by Indonesian people as traditional medicine to heal wounds, treat worm infection, and treat acne. The objective of this study was to identify the antibacterial activity of Leucaena leucocephala seed extract against Staphylococcus aureus.This was a laboratory experimental study using dilution method as many as five replications. Each replication used Leucaena leucocephala seed extract in concentrations of 3.125%, 6.25%, 12.5%, 25%, 50%, and 100% to determine the minimal inhibitory concentration (MIC) and the minimal bactericidal concentration (MBC) against Staphylococcus aureus. The results were not able to determine the minimal inhibitory concentration (MIC) of Leucaena leucocephala seed extract against Staphylococcus aureus due to dark color of the extract. While the minimal bactericidal concentration (MBC) of Leucaena leucocephala seed extract against Staphylococcus aureus was 50%. This showed that the concentration of 50% was the lowest concentration where there was no growth of the bacteria Staphylococcus aureus.In conclusion, the extract of Leucaena leucocephala seed has antibacterial activity against Staphylococcus aureus. Keywords: Staphylococcus aureus, Leucaena leucocephala, dilution method

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A safe, efficient and environment friendly biosynthesis of silver nanoparticles using Leucaena leucocephala seed extract and its antioxidant, antimicrobial, antifungal activities and potential in sensing

nano Online ◽

10.1515/nano.0015.00020 ◽

2018 ◽

Author(s):

Rajeev Kumar ◽

Priyanka Sharma ◽

Aditi Bamal ◽

Sushma Negi ◽

Savita Chaudhary

Keyword(s):

Silver Nanoparticles ◽

Leucaena Leucocephala ◽

Seed Extract ◽

Environment Friendly ◽

Antifungal Activities

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Optimization Study of Leucaena leucocephala Seed Extract as Natural Coagulant on Decolorization of Aqueous Congo Red Solutions

Arabian Journal for Science and Engineering ◽

10.1007/s13369-020-05008-1 ◽

2020 ◽

Author(s):

Jovan Kristanda ◽

Kenneth Sandrosa Sintiago ◽

Hans Kristianto ◽

Susiana Prasetyo ◽

Asaf K. Sugih

Keyword(s):

Congo Red ◽

Leucaena Leucocephala ◽

Seed Extract ◽

Natural Coagulant ◽

Optimization Study

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Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d210328 ◽

2020 ◽

Vol 21 (3) ◽

Author(s):

Sri Wardatun ◽

Yahdiana Harahap ◽

Abdul Mun'im ◽

NOORWATI SUTANDYO

Keyword(s):

Leucaena Leucocephala ◽

Room Temperature ◽

Short Communication ◽

Ethanol Concentration ◽

Seed Extract ◽

Communication Optimization ◽

Significant Difference ◽

Therapeutic Properties ◽

Sulfhydryl Compounds ◽

Leguminous Seeds

Abstract. Wardatun S, Harahap Y, Mun’im A. 2020. Short Communication: Optimization of extraction of sulfhydryl compounds from several legumes seeds in Indonesia with various ethanol concentrations. Biodiversitas 21: 1060-1064. Leucaena leucocephala (Lam.) de Wit (petai Cina), Parkia speciosa Hassk. (petai), and Archidendron jiringa (Jack) I.C. Nielsen (jengkol) seeds extracts contain sulfhydryl compounds and have various therapeutic properties. The main objective of this research was to compare the effect of various ethanol concentration on the yield of extract and levels of sulfhydryl compounds from leguminous seeds. Dried seeds were macerated at room temperature (25°C) with a ratio of solids and solvents was 1:10. The solvent concentrations used were 30%, 50%, 70% and 96% ethanol. The yield of extract was expressed as the ratio of the weight of extract to the weight of dried seeds, the level of sulfhydryl compound was expressed as reduced L-glutathione (GSH) equivalent. The level of sulfhydryl compounds was determined by Ellman reagent and further analyzed using a spectrophotometer at 411 nm wavelength. Stink bean produced the highest yield of extract, while the highest level of sulfhydryl compounds was obtained from petai cina seed extract. The concentration of ethanol gave a significant difference to the yield of extract and the level of sulfhydryl compounds. The results showed that ethanol concentration affected the efficiency of the extraction of total sulfhydryl compounds and the yield of the extract on leguminous seeds.

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