scholarly journals Identification and Characterization of S-RNases in Japanese Plum Genotypes

2019 ◽  
Vol 11 (11) ◽  
pp. 233
Author(s):  
Rauny O. de Souza ◽  
Marco A. Dalbó ◽  
Eugenia J. B. Braga ◽  
Valmor J. Bianchi
Keyword(s):  
Coding Region ◽  
Specific Primers ◽  
Allelic Profile ◽  
Japanese Plum ◽  
S Alleles ◽  
Group 3 ◽  
Group 2 ◽  
Identification And Characterization ◽  
Group 1

Many species of the genus Prunus exhibit the gametophytic self-incompatibility system, governed by the S-locus, that encode S-Ribonucleases in the pistil, and are able to degrade the RNA of the pollen tubes when the S-haplotypes of both gametophytes are the same, preventing the fertilization of the oosphere. The objective of this study was to identify and characterize the S-alleles of Japanese Plum genotypes to verify groups of reproductive compatibility. Isolation, amplification and DNA sequencing were performed to obtain an allelic profile of the genotypes. Combinations of PC1 and PC2 primers identified 95% of the genotypes. After sequencing, the ‘Sc’, ‘Si’, ‘Sa’ and ‘Sh’ alleles were obtained with an identity greater than 90%, compared to the NCBI sequences. PC2 was more extensive in identifying the S-alleles in the S-RNases coding region, generating larger fragments than PC1. In this way, it was possible to generate three groups of genotypes with S-alleles of the same size with PC2: Group 1 (Selection Embrapa A19, Selection Embrapa A28, Black Amber Black, Fortune, Roysum, SC-7 and Zafira); and Group 2: (Selection Embrapa A7, Carazinho, Sanguínea, Laroda, SC-15 and Rebelatto) and Group 3 (Selection Ameixa 86-13, Golden King, Letícia and Robusto). Each primer combination amplified only one allele per genotype, suggesting the development of specific primers to amplify the S-alleles in each genotype. The identification and characterization of the alleles allow the use of genotypes compatible with each other, considering the floral synchrony of the genotypes, besides providing information for the management of the breeding processes in Japanese Plums.

scholarly journals Morphological Characterization of Isolates of Phomopsis vexans of Bangladesh

2013 ◽  
Vol 8 (1-2) ◽  
pp. 123-130
Author(s):  
MM Islam ◽  
M Asaduzzaman ◽  
ME Hoque ◽  
MB Meah
Keyword(s):  
Morphological Characterization ◽  
Cultural Properties ◽  
Group 3 ◽  
Group 5 ◽  
Group 2 ◽  
Phomopsis Vexans ◽  
Group 1

Forty four isolates of Phomopsis vexans from different eggplant cultivars collected from core eggplant growing regions of Bangladesh which were characterized using mycological characteristics. Variation exists among the isolates of Phomopsis vexans of Bangladesh covering two types of farm having two ecosystems and the isolates were grouped into five distinct groups based on their cultural properties. The highest sized ? conidia were observed in group -1 and the lowest size in-group -2. The highest sized ? conidia were recorded in group -5 and the lowest in-group –2. The highest pycnidial size noted in-group -3 and the smallest sized pycnidia were recorded in group –1. DOI: http://dx.doi.org/10.3329/jsf.v8i1-2.14635 J. Sci. Foundation, 8(1&2): 123-130, June-December 2010

scholarly journals Vegetative Characterization of Wild Grapevines (Vitis spp.) Native to Puebla, Mexico

HortScience ◽  
2008 ◽  
Vol 43 (7) ◽  
pp. 1991-1995 ◽  
Author(s):  
Omar Franco-Mora ◽  
Edgar Jesús Morales-Rosales ◽  
Andrés González-Huerta ◽  
Juan Guillermo Cruz-Castillo
Keyword(s):  
Euclidean Distance ◽  
Principal Component ◽  
Dorsal Side ◽  
General Shape ◽  
Group 4 ◽  
Total Variability ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Thirty-four accessions of wild grapevines, native to three regions of the state of Puebla, Mexico, that is Teziutlán, Tehuacán, and Atlixco, were vegetatively characterized with 32 suggested IPGRI, UPOV, and OIV descriptors related to young shoot (YS), mature shoot (MS), young leaf (YL), mature leaf (ML), and woody shoot (WS). Excepting profile and absence of sinus teeth in ML, the remaining parameters showed variation among the accessions. After the performance of a correlation analysis among the 32 characters, only those closely correlated were used; that is, for MS: color of dorsal side of node, density of prostrate trichomes of internode; for YL: density of prostrate trichomes between veins and density of erect trichomes on main veins; and for ML: general shape of petiole sinus and the density of erect trichomes on veins in mature leaves. With those six characters, six principal components explained the total variability observed. The most closely related descriptors for principal Component 1 were the ML general shape of petiole sinus (−0.735) and the YL density of erect trichomes on main veins (0.659), whereas for principal Component 2, the MS color of the dorsal side of the node (0.677) and the density of erect trichomes in ML (0.596) were the most highly related. A cluster analysis identified four groups with a euclidean distance of 18; except for plant 183, the other six included in Group 1 were native to the regions Atlixco and Tehuacán; Group 2 was formed by plants native to the three regions, whereas Group 3 was formed by plants native to Teziutlán and Atlixco; Group 4 was formed only by Plant 36, native to Atlixco. These results show that variation among accessions occurs within and among the three states of the Puebla region.

scholarly journals Detection and in vitro and in vivo characterization of porcine circovirus DNA from a porcine-derived commercial pepsin product

2004 ◽  
Vol 85 (11) ◽  
pp. 3377-3382 ◽  
Author(s):  
M. Fenaux ◽  
T. Opriessnig ◽  
P. G. Halbur ◽  
Y. Xu ◽  
B. Potts ◽  
...  
Keyword(s):  
Porcine Circovirus Type ◽  
Human Infection ◽  
Porcine Circovirus ◽  
Specific Pathogen ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

Non-pathogenic porcine circovirus type 1 (PCV1) and pathogenic PCV2 are widespread in swine herds. In this study, the detection and characterization of PCV1 and PCV2 DNA from porcine-derived commercial pepsin are reported. The complete genomic sequences of the pepsin-derived PCV1 and PCV2 share 76 % nucleotide sequence identity with each other and 95–99 % identity with respective North American PCV1 and PCV2 isolates. However, the PCV-contaminated pepsin lacks infectivity in PK-15 cells. To further assess the infectivity of the contaminating pepsin in vivo, 16 5-week-old, specific-pathogen-free pigs were divided randomly into three groups: pigs in group 1 (n=5) were each inoculated intramuscularly and intranasally with 4 ml PBS buffer as negative controls, those in group 2 (n=6) were each inoculated with 400 mg contaminated pepsin dissolved in 4 ml PBS and those in group 3 (n=5) were each inoculated with 4×104·3 TCID50 PCV2 as positive controls. PCV2 viraemia, seroconversion and pathological lesions were detected in group 3 pigs, but not in group 1 or 2 pigs, confirming that the contaminating PCVs were non-infectious. Nevertheless, the detection of PCV DNA in a porcine-derived commercial product raises concern for potential human infection through xenotransplantation.

scholarly journals Quinine-Dependent Antibodies Bind a Restricted Set of Epitopes on the Glycoprotein Ib-IX Complex: Characterization of the Epitopes

Blood ◽  
1998 ◽  
Vol 92 (7) ◽  
pp. 2366-2373 ◽  
Author(s):  
Janette K. Burgess ◽  
Jose A. Lopez ◽  
Michael C. Berndt ◽  
Ian Dawes ◽  
Colin N. Chesterman ◽  
...  
Keyword(s):  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Membrane Glycoprotein ◽  
Ovary Cells ◽  
Group 3 ◽  
Complex Characterization ◽  
Group 2 ◽  
Inhibition Studies ◽  
Group 1

Abstract Severe immune thrombocytopenia is an idiosyncratic complication of quinine therapy. Although in most cases the responsible antibody is directed against platelet membrane glycoprotein (GP) Ib-IX, specificity for GPIIb-IIIa or both epitopes has also been reported. The objective of this study was to characterize the binding site of GPIb-IX–specific quinine-dependent antibodies. Antibody binding to Chinese hamster ovary cells or mouse L cells stably transfected with various combinations of the three genes (Ibα, Ibβ, or IX) that encode this complex was detected using flow cytometry, monoclonal antibody–specific immobilization of platelet antigens assay, and differential adsorption studies. IgG in sera from 15 patients with quinine-induced thrombocytopenia binding to the cells, in the presence of quinine, showed three distinct patterns. Group 1 sera contained at least two antibody populations, one which binds to GPIbα and another which recognizes GPIX. Group 2 sera contained an antibody which binds drug dependently to GPIX, and Group 3 sera contained an antibody which recognizes a quinine-dependent epitope on GPIbα. Thus, the quinine-dependent antibodies fall into two distinct populations that bind to GPIbα and GPIX independently. Using proteases which cleave GPIbα at specific sites, we have shown that the GPIbα-specific antibody binds to an 11–amino acid (283 to 293) region. Peptide inhibition studies provide confirmatory evidence that this region contains the epitope for the GPIbα-specific quinine-dependent antibody.

scholarly journals Quinine-Dependent Antibodies Bind a Restricted Set of Epitopes on the Glycoprotein Ib-IX Complex: Characterization of the Epitopes

Blood ◽  
1998 ◽  
Vol 92 (7) ◽  
pp. 2366-2373 ◽  
Author(s):  
Janette K. Burgess ◽  
Jose A. Lopez ◽  
Michael C. Berndt ◽  
Ian Dawes ◽  
Colin N. Chesterman ◽  
...  
Keyword(s):  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Membrane Glycoprotein ◽  
Ovary Cells ◽  
Group 3 ◽  
Complex Characterization ◽  
Group 2 ◽  
Inhibition Studies ◽  
Group 1

Severe immune thrombocytopenia is an idiosyncratic complication of quinine therapy. Although in most cases the responsible antibody is directed against platelet membrane glycoprotein (GP) Ib-IX, specificity for GPIIb-IIIa or both epitopes has also been reported. The objective of this study was to characterize the binding site of GPIb-IX–specific quinine-dependent antibodies. Antibody binding to Chinese hamster ovary cells or mouse L cells stably transfected with various combinations of the three genes (Ibα, Ibβ, or IX) that encode this complex was detected using flow cytometry, monoclonal antibody–specific immobilization of platelet antigens assay, and differential adsorption studies. IgG in sera from 15 patients with quinine-induced thrombocytopenia binding to the cells, in the presence of quinine, showed three distinct patterns. Group 1 sera contained at least two antibody populations, one which binds to GPIbα and another which recognizes GPIX. Group 2 sera contained an antibody which binds drug dependently to GPIX, and Group 3 sera contained an antibody which recognizes a quinine-dependent epitope on GPIbα. Thus, the quinine-dependent antibodies fall into two distinct populations that bind to GPIbα and GPIX independently. Using proteases which cleave GPIbα at specific sites, we have shown that the GPIbα-specific antibody binds to an 11–amino acid (283 to 293) region. Peptide inhibition studies provide confirmatory evidence that this region contains the epitope for the GPIbα-specific quinine-dependent antibody.

Treatment outcome and functional characterization of uncommon EGFR mutations in the German National Network Genomic Medicine Lung Cancer (nNGM).

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. 9036-9036
Author(s):  
Melanie Janning ◽  
Juliane Sueptitz ◽  
Anika Forstreuter ◽  
Corinna Albers-Leischner ◽  
Amanda Tufman ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Egfr Mutations ◽  
Group 3 ◽  
Group 2 ◽  
Exon 20 ◽  
Egfr Tki ◽  
Egfr Tkis ◽  
Group 1

9036 Background: The nNGM centralizes molecular diagnostics, treatment recommendations and follow-up reporting in NSCLC in Germany. Uncommon EGFR mutations pose a clinical challenge because they comprise a heterogenous group and analyses of treatment outcome are still scarce. Here, we analyzed follow-up data of patients with rare EGFR mutations and performed functional characterization of recurrent mutations with unknown function. Methods: This multicenter, retrospective analysis of uncommon EGFR mutations (excluding L858R-, T790M mutations and exon 19 deletions) includes stage IV patients with NSCLC from 12 nNGM centers. We categorized EGFR-mutations into 3 groups: uncommon EGFR mutations with known driver function, for instance E709X, G719X, S768I and L861Q (group 1), exon 20 insertions (group 2) and all other very rare mutations (group 3). Functional characterization of unknown mutations was performed by insertion mutagenesis in Ba/F3 cells and monitoring of growth factor-independent proliferation. Results: In total, 834 cases with uncommon EGFR mutations were reported. Follow-up data after EGFR-TKI (Erlotinib, Gefitinib, Afatinib and Osimertinib), chemotherapy and/or mono-PD(L)1 blockade was available for 252 patients. Mean progression free survival (mPFS) on EGFR-TKIs vs. chemotherapy was 6.6 months vs. 5.0 months (HR 0.54, 95%CI 0.35 to 0.81, P =.003) in group 1 (n = 84), and 6.7 months vs. 3.4 months (HR 0.66, 95%CI 0.47 to 0.92, P =.015) in group 3 (n = 104). Mono-anti-PD(L1) blockade was not superior to chemotherapy (group 1, mPFS 3.0 months, HR 1.32, 95% 0.55 – 3.15, P =.535 and group3, mPFS 4.3 months, HR 1.02, 95% CI 0.64 – 1.62, P = 0.951). Exon 20 insertions (group 2, n = 63) did not benefit from EGFR-TKIs or anti-PD(L1) blockade vs. chemotherapy. Overall survival (OS) analysis (n = 218) following chemotherapy (56%) or EGFR-TKI treatment (44%) showed median OS (mOS) of 18.0 months vs. 13.9 months in patients treated with EGFR-TKI and chemotherapy, respectively in group 1 (HR 0.97, 95%CI 0.54 to 1.75, P =.929). In group 3 patients treated with EGFR-TKI and chemotherapy had a mOS of 35.4 months vs. 12.0 months, respectively (HR 0.59, 95%CI 0.35 to 1.01, P =.056). In the Ba/F3 system we could identify 8 recurrent driver and 12 non-driver mutations with a clinically applicable assay turnaround time of 4 weeks to inform clinical decision-making in the future. Conclusions: This real-world dataset confirms that patients with group 1(uncommon) EGFR mutations benefit from EGFR-TKIs and indicates that mono-anti PD(L)1 blockade is not superior to chemotherapy. Furthermore, patients with very rare EGFR mutations (group 3) also experienced a PFS benefit from EGFR-TKI compared to chemotherapy while immune therapy was not beneficial.

In-vivo evaluation of the effect of cyanoacrylate on prosthetic vascular graft infection – does cyanoacrylate increase the severity of infection?

VASA ◽  
2020 ◽  
Vol 49 (4) ◽  
pp. 281-284
Author(s):  
Atıf Yolgosteren ◽  
Gencehan Kumtepe ◽  
Melda Payaslioglu ◽  
Cuneyt Ozakin
Keyword(s):  
Vascular Graft ◽  
Graft Infection ◽  
Vascular Graft Infection ◽  
Group 4 ◽  
Significant Difference ◽  
Group 3 ◽  
Group 2 ◽  
Prosthetic Vascular Graft Infection ◽  
Group 1

Summary. Background: Prosthetic vascular graft infection (PVGI) is a complication with high mortality. Cyanoacrylate (CA) is an adhesive which has been used in a number of surgical procedures. In this in-vivo study, we aimed to evaluate the relationship between PVGI and CA. Materials and methods: Thirty-two rats were equally divided into four groups. Pouch was formed on back of rats until deep fascia. In group 1, vascular graft with polyethyleneterephthalate (PET) was placed into pouch. In group 2, MRSA strain with a density of 1 ml 0.5 MacFarland was injected into pouch. In group 3, 1 cm 2 vascular graft with PET piece was placed into pouch and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. In group 4, 1 cm 2 vascular graft with PET piece impregnated with N-butyl cyanoacrylate-based adhesive was placed and MRSA strain with a density of 1 ml 0.5 MacFarland was injected. All rats were scarified in 96th hour, culture samples were taken where intervention was performed and were evaluated microbiologically. Bacteria reproducing in each group were numerically evaluated based on colony-forming unit (CFU/ml) and compared by taking their average. Results: MRSA reproduction of 0 CFU/ml in group 1, of 1410 CFU/ml in group 2, of 180 200 CFU/ml in group 3 and of 625 300 CFU/ml in group 4 was present. A statistically significant difference was present between group 1 and group 4 (p < 0.01), between group 2 and group 4 (p < 0.01), between group 3 and group 4 (p < 0.05). In terms of reproduction, no statistically significant difference was found in group 1, group 2, group 3 in themselves. Conclusions: We observed that the rate of infection increased in the cyanoacyrylate group where cyanoacrylate was used. We think that surgeon should be more careful in using CA in vascular surgery.

Abnormal Haemostasis and Blood Viscosity in Malignant Hypertension

1984 ◽  
Vol 52 (03) ◽  
pp. 253-255 ◽  
Author(s):  
C Isles ◽  
G D O Lowe ◽  
B M Rankin ◽  
C D Forbes ◽  
N Lucie ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Blood Viscosity ◽  
Renal Damage ◽  
Antithrombin Iii ◽  
Plasma Viscosity ◽  
Malignant Hypertension ◽  
Fibrin Deposition ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

SummaryWe have previously shown abnormalities of haemostasis suggestive of intravascular coagulation in patients with malignant hypertension, a condition associated with retinopathy and renal fibrin deposition. To determine whether such abnormalities are specific to malignant hypertension, we have measured several haemostatic and haemorheological variables in 18 patients with malignant hypertension (Group 1), 18 matched healthy controls (Group 2), and 18 patients with non-malignant hypertension (Group 3) matched for renal pathology, blood pressure and serum creatinine with Group 1. Both Groups 1 and 3 had increased mean levels of fibrinogen, factor VIIIc, beta-thrombo- globulin, plasma viscosity and blood viscosity (corrected for haematocrit); and decreased mean levels of haematocrit, antithrombin III and platelet count. Mean levels of fast antiplasmin and alpha2-macroglobulin were elevated in Group 1 but not in Group 3. We conclude that most blood abnormalities are not specific to malignant hypertension; are also present in patients with non-malignant hypertension who have similar levels of blood pressure and renal damage; and might result from renal damage as well as promoting further renal damage by enhancing fibrin deposition. However increased levels of fibrinolytic inhibitors in malignant hypertension merit further investigation in relation to removal of renal fibrin.

Features of postoperative period in patients with chronic venous insufficiency: phlebectomy versus thermal ablation

2020 ◽  
pp. 64-75
Author(s):  
E. Burleva ◽  
O. Smirnov ◽  
S. Tyurin
Keyword(s):  
Postoperative Period ◽  
Chronic Venous Insufficiency ◽  
Thermal Ablation ◽  
Venous Insufficiency ◽  
Clinical Symptoms ◽  
Varicose Veins ◽  
Statistical Processing ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

The purpose of the study was to conduct a comparative assessment of the course of the postoperative period after phlebectomy and thermal ablation in patients with varicose veins of the lower extremities in the system of the great saphenous vein (GSV) with class C2 of chronic venous insufficiency (CVI) — CEAP class C2. Materials and methods: 455 patients (455 limbs) with CEAP class C2. Group 1 (n = 154) received stripping + minimally invasive phlebectomy; Group 2 — endovenous laser ablation (EVLA) of GSV trunk + sclerotherapy of varicose veins; 3 group (n = 150) — radiofrequency ablation (RFA) of the GSV + sclerotherapy. All patients were united by a single tactical solution — the elimination of pathological vertical reflux in GSV. In each group, patients were with similar hemodynamic profile were selected (Group 1 = 63; Group 2 = 61; Group 3 = 61). The course of the postoperative period (from 2 days to 2 months) was compared for pain (visual analog scale — VAS), clinical symptoms of chronic venous insufficiency, degree of satisfaction (Darvall questionnaire), and duration of disability. Statistical processing was carried out using Excel programs for Windows XP, MedCalc® (version 11.4.2.0., Mariakerke, Belgium). Results: Postoperative pain is more pronounced (during day 1 for Group 1–4.0, Group 2–3.0, Group 3–2.0) and more prolonged (up to 4 days) after open surgeries (p < 0.05). The dynamics of the clinical symptoms of CVI (including varicose syndrome and use of compression therapy) could not be fully evaluated in connection with the ongoing sclerotherapy procedures for patients of Groups 2 and 3. Satisfaction of patients with aesthetic aspects was higher than expected in all groups. Reliable statistical differences proved decrease in days of disability (Group 1–14; Group 2–4; Group 3–3) and earlier return to physical activities and work in patients after thermal ablation in comparison with phlebectomy. Conclusion: The study shows that all three methods for eliminating vertical reflux in the GSV can be proposed for a large category of patients with CEAP of class C3 and C2. Medical and social rehabilitation of patients using endovascular thermal ablation technologies proceeds faster, which is beneficial both for the patients and for society.

Analysis of the quality of early diagnostics of cardiovascular diseases in students of educational institutions of Khabarovsk (according to the data of preventive examinations)

2020 ◽  
pp. 13-16
Keyword(s):  
Blood Pressure ◽  
Cardiovascular Diseases ◽  
Sinus Tachycardia ◽  
Blood Pressure Level ◽  
Educational Institutions ◽  
Conduction Disturbances ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

To identify the prevalence of early pathology of cardiovascular diseases, a survey of 400 200 girls) in the age group 15 and 17 years old was conducted as a part of routine medical of the level of blood pressure (BP) was carried out, with the calculation of the average level pressure on the basis of three separate measurements estimated by percentile tables for a registration of a standard resting ECG in 12 leads. According to the results of the survey, into 3 groups: with an increase in blood pressure above 95 ‰ (group 1 – 16 people), which recorded in males (p<0,05); Group 2 (67 people) – adolescents with a normal blood pressure level and group 3 of adolescents with a decrease in blood pressure below 5 ‰ changes in the form of rhythm and conduction disturbances were noted in almost every a predominance of sinus tachycardia in the first group. In the third group of adolescents, form of ectopic rhythm and pacemaker migration were significantly more frequently only 78 % of adolescents were referred for consultation and in-depth examination by a pediatric cardiologist.

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