scholarly journals Plasma Lipid Peroxidation as a Marker for Seminal Oxidative Stress in Stallion

2019 ◽  
Vol 11 (6) ◽  
pp. 401
Author(s):  
Patricia Wolkmer ◽  
Andressa M. G. Stumm ◽  
Luiz F. K. Borges ◽  
Eduarda P. T. Ferreira ◽  
Bruna Favaretto ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Seminal Plasma ◽  
Plasma Lipid ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Semen Collection ◽  
Semen Storage ◽  
Plasma Lipid Peroxidation ◽  
Antioxidant Enzyme Catalase

This experiment aims to evaluate the correlation between lipid peroxidation levels in serum and seminal plasma in equines. Also, it investigates the lipid peroxidation in extended semen samples and its effects and sperm motility during a 72 hr refrigeration period. Blood and semen were collected from fertile Crioulo stallions. Serum and seminal plasma lipid peroxidation levels were analyzed by thiobarbituric acid reactive substances (TBARS) immediately after semen collection. After addition of extender (hour = 0), diluted semen was refrigerated and stored at 5 °C. Semen analyses, TBARS and catalase activity were performed in extended semen at 0, 24, 48, and 72 hours. We noted that levels of plasma lipid peroxidation can be used as an indicative of seminal oxidative stress. Also, lipid peroxidation does not increase substantially during semen storage. Lipid peroxidation and the antioxidant enzyme catalase do not seem to be the major cause of loss and motility and consequently reduction in fertility in stallion semen during storage for 72 h at 5 °C.

scholarly journals Application of FTIR-ATR Spectroscopy to Determine the Extent of Lipid Peroxidation in Plasma during Haemodialysis

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Adam Oleszko ◽  
Sylwia Olsztyńska-Janus ◽  
Tomasz Walski ◽  
Karolina Grzeszczuk-Kuć ◽  
Jolanta Bujok ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Plasma Lipid ◽  
Thiobarbituric Acid ◽  
Breakdown Product ◽  
Thiobarbituric Acid Reactive Substances ◽  
Reflectance Ftir ◽  
The Fourier Transform ◽  
Atr Spectroscopy ◽  
Plasma Lipid Peroxidation

During a haemodialysis (HD), because of the contact of blood with the surface of the dialyser, the immune system becomes activated and reactive oxygen species (ROS) are released into plasma. Particularly exposed to the ROS are lipids and proteins contained in plasma, which undergo peroxidation. The main breakdown product of oxidized lipids is the malondialdehyde (MDA). A common method for measuring the concentration of MDA is a thiobarbituric acid reactive substances (TBARS) method. Despite the formation of MDA in plasma during HD, its concentration decreases because it is removed from the blood in the dialyser. Therefore, this research proposes the Fourier Transform Infrared Attenuated Total Reflectance (FTIR-ATR) spectroscopy, which enables determination of primary peroxidation products. We examined the influence of the amount of hydrogen peroxide added to lipid suspension that was earlier extracted from plasma specimen on lipid peroxidation with use of TBARS and FTIR-ATR methods. Linear correlation between these methods was shown. The proposed method was effective during the evaluation of changes in the extent of lipid peroxidation in plasma during a haemodialysis in sheep. A measurement using the FTIR-ATR showed an increase in plasma lipid peroxidation after 15 and 240 minutes of treatment, while the TBARS concentration was respectively lower.

scholarly journals Polyphenols from Berries ofAronia melanocarpaReduce the Plasma Lipid Peroxidation Induced by Ziprasidone

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Anna Dietrich-Muszalska ◽  
Justyna Kopka ◽  
Bogdan Kontek
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Healthy Subjects ◽  
Plasma Lipid ◽  
Thiobarbituric Acid ◽  
Tbars Level ◽  
Polyphenol Compounds ◽  
Aronia Melanocarpa ◽  
Plasma Lipid Peroxidation

Background. Oxidative stress in schizophrenia may be caused partially by the treatment of patients with antipsychotics. The aim of the study was to establish the effects of polyphenol compounds derived from berries ofAronia melanocarpa(Aronox) on the plasma lipid peroxidation induced by ziprasidonein vitro.Methods. Lipid peroxidation was measured by the level of thiobarbituric acid reactive species (TBARS). The samples of plasma from healthy subjects were incubated with ziprasidone (40 ng/ml; 139 ng/ml; and 250 ng/ml) alone and with Aronox (5 ug/ml; 50 ug/ml).Results. We observed a statistically significant increase of TBARS level after incubation of plasma with ziprasidone (40 ng/ml; 139 ng/ml; and 250 ng/ml) (after 24 h incubation:P=7.0× 10−4,P=1.6× 10−3, andP=2.7× 10−3, resp.) and Aronox lipid peroxidation caused by ziprasidone was significantly reduced. After 24-hour incubation of plasma with ziprasidone (40 ng/ml; 139 ng/ml; and 250 ng/ml) in the presence of 50 ug/ml Aronox, the level of TBARS was significantly decreased:P=6.5× 10−8,P=7.0× 10−6, andP=3.0× 10−5, respectively.Conclusion. Aronox causes a distinct reduction of lipid peroxidation induced by ziprasidone.

scholarly journals Gene expression in human small intestinal mucosa in vivo is mediated by iron-induced oxidative stress

2006 ◽  
Vol 25 (2) ◽  
pp. 242-249 ◽  
Author(s):  
Freddy J. Troost ◽  
Robert-Jan M. Brummer ◽  
Guido R. M. M. Haenen ◽  
Aalt Bast ◽  
Rachel I. van Haaften ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Lipid Peroxidation ◽  
Small Intestine ◽  
Antioxidant Capacity ◽  
Intestinal Mucosa ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substances ◽  
Oxidative Challenge ◽  
Gene Reporters

Iron-induced oxidative stress in the small intestine may alter gene expression in the intestinal mucosa. The present study aimed to determine which genes are mediated by an iron-induced oxidative challenge in the human small intestine. Eight healthy volunteers [22 yr(SD2)] were tested on two separate occasions in a randomized crossover design. After duodenal tissue sampling by gastroduodenoscopy, a perfusion catheter was inserted orogastrically to perfuse a 40-cm segment of the proximal small intestine with saline and, subsequently, with either 80 or 400 mg of iron as ferrous gluconate. After the intestinal perfusion, a second duodenal tissue sample was obtained. Thiobarbituric acid-reactive substances, an indicator of lipid peroxidation, in intestinal fluid samples increased significantly and dose dependently at 30 min after the start of perfusion with 80 or 400 mg of iron, respectively ( P < 0.001). During the perfusion with 400 mg of iron, the increase in thiobarbituric acid-reactive substances was accompanied by a significant, momentary rise in trolox equivalent antioxidant capacity, an indicator of total antioxidant capacity ( P < 0.05). The expression of 89 gene reporters was significantly altered by both iron interventions. Functional mapping showed that both iron dosages mediated six distinct processes. Three of those processes involved G-protein receptor coupled pathways. The other processes were associated with cell cycle, complement activation, and calcium channels. Iron administration in the small intestine induced dose-dependent lipid peroxidation and a momentary antioxidant response in the lumen, mediated the expression of at least 89 individual gene reporters, and affected at least six biological processes.

scholarly journals Fortified Extract of Red Berry,Ginkgo biloba, and White Willow Bark in Experimental Early Diabetic Retinopathy

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Claudio Bucolo ◽  
Giuseppina Marrazzo ◽  
Chiara Bianca Maria Platania ◽  
Filippo Drago ◽  
Gian Marco Leggio ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Diabetic Retinopathy ◽  
Ginkgo Biloba ◽  
Plasma Lipid ◽  
Thiobarbituric Acid ◽  
Diabetic Rats ◽  
Sprague Dawley ◽  
White Willow ◽  
Willow Bark

Diabetic retinopathy is a complex condition where inflammation and oxidative stress represent crucial pathways in the pathogenesis of the disease. Aim of the study was to investigate the effects of a fortified extract of red berries,Ginkgo bilobaand white willow bark containing carnosine andα-lipoic acid in early retinal and plasma changes of streptozotocin-induced diabetic rats. Diabetes was induced by a single streptozotocin injection in Sprague Dawley rats. Diabetics and nondiabetic (control) rats were treated daily with the fortified extract for the ten days. Retina samples were collected and analyzed for their TNF-αand VEGF content. Moreover, plasma oxidative stress was evaluated by thiobarbituric acid reacting substances (TBARS). Increased TNF-αand VEGF levels were observed in the retina of diabetic rats. Treatment with the fortified extract significantly lowered retinal cytokine levels and suppressed diabetes-related lipid peroxidation. These data demonstrate that the fortified extract attenuates the degree of retinal inflammation and plasma lipid peroxidation preserving the retina in early diabetic rats.

Protective effect of chokeberry on chemical-induced oxidative stress in rat

2010 ◽  
Vol 30 (3) ◽  
pp. 199-208 ◽  
Author(s):  
Małgorzata Kujawska ◽  
Ewa Ignatowicz ◽  
Małgorzata Ewertowska ◽  
Jan Oszmiański ◽  
Jadwiga Jodynis-Liebert
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Wistar Rats ◽  
Thiobarbituric Acid ◽  
Protein Carbonyls ◽  
Thiobarbituric Acid Reactive Substances ◽  
Blood Leukocytes ◽  
Microsomal Lipid Peroxidation ◽  
Male Wistar Rats ◽  
Chokeberry Juice

Male Wistar rats were treated with chokeberry juice per os, 10 mL/kg/day, for 28 days and a single intraperitoneal (i.p.) dose of N-nitrosodiethylamine (NDEA), 150 mg/kg, or carbon tetrachloride (CCl4), 2 ml/kg. The level of hepatic microsomal lipid peroxidation, expressed as thiobarbituric acid reactive substances (TBARS), was increased in animals dosed with NDEA and CCl4. Juice pretreatment resulted in a significant decrease in TBARS by 53% and 92%, respectively. In rats administered juice alone, 50% decrease in TBARS was noted. The activities of all antioxidant enzymes were decreased in the liver of rats administered either toxicant by 29%—52% as compared to controls. Juice pretreatment resulted in an increase in the activity of catalase, glutathione peroxidase and glutathione reductase by 117%, 56% and 44%, respectively, only in rats challenged with NDEA. Although no response of plasma protein carbonyls to both toxicants was observed, the pretreatment with juice caused a 55% decrease of this parameter in CCl4—dosed rats. DNA damage in blood leukocytes induced by either toxicant was slightly reduced, by 24%, in the rats pretreated with juice and administered NDEA. The results of the study showed that pretreatment with chokeberry juice confers some protection against chemical-induced oxidative stress.

scholarly journals Hippophae rhamnoidesL. Fruits Reduce the Oxidative Stress in Human Blood Platelets and Plasma

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Beata Olas ◽  
Bogdan Kontek ◽  
Paulina Malinowska ◽  
Jerzy Żuchowski ◽  
Anna Stochmal
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Human Blood ◽  
Blood Platelets ◽  
Plasma Lipid ◽  
Protein Carbonylation ◽  
Phenolic Fraction ◽  
Plasma Lipid Peroxidation ◽  
Promising Source ◽  
Human Blood Platelets

Effects of the phenolic fraction fromHippophae rhamnoidesfruits on the production of thiobarbituric acid reactive substances (TBARS, a marker of lipid peroxidation) and the generation of superoxide anion (O2-∙) in human blood platelets (resting platelets and platelets stimulated by a strong physiological agonist, thrombin) were studiedin vitro. We also examined antioxidant properties of this fraction against human plasma lipid peroxidation and protein carbonylation induced by a strong biological oxidant, hydrogen peroxide (H2O2) or H2O2/Fe (a donor of hydroxyl radicals). The tested fraction ofH. rhamnoides(0.5– 50 µg/mL; the incubation time: 15 and 60 min) inhibited lipid peroxidation induced by H2O2or H2O2/Fe. TheH. rhamnoidesphenolic fraction inhibited not only plasma lipid peroxidation, but also plasma protein carbonylation stimulated by H2O2or H2O2/Fe. Moreover, the level ofO2-∙in platelets significantly decreased. In comparative experiments, theH. rhamnoidesfraction was a more effective antioxidant than aronia extract or grape seed extract (at the highest tested concentration, 50 µg/mL). The obtained results suggest thatH. rhamnoidesfruits may be a new, promising source of natural compounds with antioxidant and antiplatelet activity beneficial not only for healthy people, but also for those with oxidative stress-associated diseases.

Fish oil induced hyperlipidemia and oxidative stress in BioF1B hamsters is attenuated by elderberry extract

2012 ◽  
Vol 37 (3) ◽  
pp. 472-479 ◽  
Author(s):  
Pratibha Dubey ◽  
Anura P. Jayasooriya ◽  
Sukhinder K. Cheema
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Fish Oil ◽  
Thiobarbituric Acid ◽  
Omega 3 ◽  
Thiobarbituric Acid Reactive Substances ◽  
Hepatic Lipids ◽  
Dietary Fish ◽  
Cardioprotective Effects ◽  
And Oxidative Stress

We have previously reported fish oil induced hyperlipidemia in BioF1B hamsters compared with Golden Syrian (GS) hamsters. Elderberry (Sambucus nigra L.) extract is abundant in anthocyanins and is believed to exert cardioprotective effects primarily by virtue of its hypolipidemic and antioxidant potential. In the current study, high-fat fish oil feeding increased oxidative stress in BioF1B hamsters compared with GS hamsters; this increase was associated with increased levels of omega-3 polyunsaturated fatty acids in plasma and liver. We then investigated whether cosupplementation with anthocyanin-rich elderberry extract would reverse fish oil induced hyperlipidemia and reduce lipid peroxidation in BioF1B hamsters. Plasma and hepatic lipids decreased significantly when hamsters were fed diets containing elderberry extract along with fish oil. Both plasma and liver thiobarbituric acid reactive substances showed significant reductions upon cosupplementation with elderberry extract in fish oil fed BioF1B hamsters. Our findings demonstrate that cosupplementation with elderberry extract reverses hyperlipidemia and lipid peroxidation observed with dietary fish oil alone in BioF1B hamsters.

Comparison of the toxicities of cisplatin and a new cisplatin-procaine complex to rat renal cortical slices

1996 ◽  
Vol 15 (1) ◽  
pp. 59-63 ◽  
Author(s):  
J-G. Zhang ◽  
M. Esposito ◽  
S. Cafaggi ◽  
WE Lindup
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Thiobarbituric Acid ◽  
Procaine Hydrochloride ◽  
Similar Magnitude ◽  
Thiobarbituric Acid Reactive Substances ◽  
Enzyme Leakage ◽  
Mtt Reduction ◽  
Rat Renal Cortical Slices ◽  
Cortical Slices

1 Procaine has previously been shown to diminish the nephrotoxicity of cisplatin and the nephrotoxic effects of cisplatin and a new cisplatin complex (cis-diammine chloro - [2 - (diethylamino) ethyl - 4 - aminobenzoate, N4]- chlorideplatinum (II) monohydrochloride monohydrate; DPR), that contains procaine hydrochloride were com pared with rat renal cortical slices. 2 Cisplatin at 1 mM caused toxicity to the slices, as shown by an increase in the leakage of aspartate aminotransfer ase and lactate dehydrogenase from the slices into the incubation medium and a decrease in the reduction of a tetrazolium dye (MTT assay). Addition of procaine (1 mM) protected against cisplatin-induced toxicity. DPR either at 1 mM or at 4 mM had no effect either on the enzyme leakage or MTT reduction by the renal slices, but DPR at 10 mM produced a similar magnitude of enzyme leakage to cisplatin (1 mM). 3 DPR lowered the concentration of ATP and glutathione (GSH) in the slices but was less potent than cisplatin. Thiobarbituric acid reactive substances, indicators of lipid peroxidation, released into the medium were increased by the highest concentration of DPR (10 mM), which suggests that DPR has the potential to cause oxidative stress. 4 The results suggest that DPR was far less toxic than either cisplatin alone or a mixture of cisplatin and procaine.

scholarly journals Thiobarbituric acid reactive substances as marker of oxidative stress in pregnancies with pre-eclampsia

2011 ◽  
Vol 64 (7-8) ◽  
pp. 377-380 ◽  
Author(s):  
Aleksandra Novakov-Mikic ◽  
Snezana Brkic ◽  
Daniela Maric ◽  
Bojan Sekulic ◽  
Aleksandar Cetkovic ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Screening Method ◽  
Systolic Arterial Pressure ◽  
Thiobarbituric Acid ◽  
Thiobarbituric Acid Reactive Substance ◽  
Thiobarbituric Acid Reactive Substances ◽  
Reactive Substance ◽  
To Come ◽  
Tbars Assay

Pre-eclampsia is characterized by increased lipid peroxidation and diminished antioxidant capacity. The aim of the study was to establish concentration of thiobarbituric acid reactive substances as a marker of lipid peroxidation in normal pregnancies and in pregnancies complicated with pre-eclampsia, and to estimate the possibility of using thiobarbituric acid reactive substances as a screening method for development of pre-eclampsia. The study was conducted at the Department of Obstetrics and Gynaecology, Clinical Centre of Vojvodina. The study included 57 singleton pregnancies, gestation >24 weeks, of which 29 were healthy pregnancies and 28 were with pre-eclampsia, defined as systolic arterial pressure of >90 mmHg, diastolic of >145 mmHg, and 24h proteinuria of >300mg. Thiobarbituric acid reactive substances concentrations evaluated by malondialdehyde equivalent standards (OxiSelect? TBARS Assay Kit (malondialdehyde Quantitation), Cell Biolabs? OxiSelect?) showed that oxidative stress was more evident in the group with pre-eclampsia, though not statistically significant (p= 0.107). There was no correlation of thiobarbituric acid reactive substance levels with gestation in either group. The differences between the level of thiobarbituric acid reactive substance concentrations in pre-eclampsia and healthy pregnancies indicate the possibility of using thiobarbituric acid reactive substances as a screening tool for the development of pre-eclampsia. Further studies with larger numbers of patients are needed in order to come to final conclusions.

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