scholarly journals Assessment of genetic diversity in sea buckthorn (Hippophae rhamnoides L.) accessions from Mongolia based on RAPD markers

Author(s):  
Zolzaya Sandag ◽  
Dorjderem Balchin ◽  
Ariya Enkhtuya ◽  
Nomin Myagmar ◽  
Nomuun Oyunbat ◽  
...  

Hippophae rhamnoides L., which belongs to the Elaegnaceae family, is one of the medically and environmentally valuable berry crops with its high nutritious and bioactive compounds. Despite its high demand in the food, medicinal and agricultural industries, this species has been less studied molecularly. In view of this, an effort has been made in the present study to characterize 24 accessions of H. rhamnoides collected from different geographical regions of Mongoliaa through random amplified polymorphic DNA (RAPD) markers. A total of 10 RAPD primers were used in the present study for their ability to produce clear, scorable amplicons. The RAPD analysis totally generated 87 bands, of which 84 (96.34%) were polymorphic, pointing to a high degree of genetic variation. The similarity coefficient ranged from 0.4-1 with the mean of 0.78. The UPGMA dendrogram was generated using these data grouped accessions into two main clusters. Cluster analysis reflected a relatively close relationship between accessions grown at the same or neighbouring areas. Thus, our data could be informative for further selection and management of germplasm collections and crossing strategies for sea buckthorn.

Plants ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 847
Author(s):  
Anita Zapałowska ◽  
Natalia Matłok ◽  
Miłosz Zardzewiały ◽  
Tomasz Piechowiak ◽  
Maciej Balawejder

The aim of this research was to show the effect of the ozonation process on the quality of sea buckthorn (Hippophae rhamnoides L.). The quality of the ozonated berries of sea buckthorn was assessed. Prior to and after the ozone treatment, a number of parameters, including the mechanical properties, moisture content, microbial load, content of bioactive compounds, and composition of volatile compounds, were determined. The influence of the ozonation process on the composition of volatile compounds and mechanical properties was demonstrated. The ozonation had negligible impact on the weight and moisture of the samples immediately following the treatment. Significant differences in water content were recorded after 7 days of storage. It was shown that the highest dose of ozone (concentration and process time) amounting to 100 ppm for 30 min significantly reduced the water loss. The microbiological analyses showed the effect of ozone on the total count of aerobic bacteria, yeast, and mold. The applied process conditions resulted in the reduction of the number of aerobic bacteria colonies by 3 log cfu g−1 compared to the control (non-ozonated) sample, whereas the number of yeast and mold colonies decreased by 1 log cfu g−1 after the application of 100 ppm ozone gas for 30 min. As a consequence, ozone treatment enhanced the plant quality and extended plant’s storage life.


2021 ◽  
pp. 130481
Author(s):  
Xingang Lyu ◽  
Xiao Wang ◽  
Qilei Wang ◽  
Xueying Ma ◽  
Suolian Chen ◽  
...  

Author(s):  
Carmen Georgeta Dumitrescu (Manole)

This paper approaches an issue of real importance, namely the sea-buckthorn influence on the areas attacked by erosion. We consider that this effect is mainly a result of its extremely developed root system, as well as of the nodosities formation on the roots. The study was conducted over a period of 3 years (2011-2013) in two landslides areas from Prahova and Dambovita counties, from where were collected 5 plants (from each area) in 3 repetitions. Calculating the averages regarding the plant roots depth, but also the number of root suckers and nodosities, can be easily deduced the role of these plants in soil fixation, hence their eco-restoration value.


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