Distinct pattern of Th17/Treg cells in pregnant women with a history of unexplained recurrent spontaneous abortion

Unexplained recurrent spontaneous abortion (URSA) is an alloimmune disease associated with the failure of fetal-maternal immunologic tolerance in which the regulatory T lymphocytes (Treg) play a pivotal role. It is well known that Forkhead box P3 (Foxp3) is a crucial regulatory factor for the development and function of Treg cells. It has also been established that deficiency of the Foxp3 gene suppresses the regulatory function of Treg cells. To determine if functional polymorphisms at the Foxp3 loci are associated with URSA in humans, we genotyped four common polymorphisms of Foxp3 gene in 146 unrelated URSA patients and 112 healthy women. The results showed that rs3761548A/C and rs2232365A/G polymorphisms were significantly associated with URSA. Additionally, we found that the allelic distribution of rs5902434 del/ATT in URSA group was slightly different from that in the control group. We conclude that functional polymorphisms of the Foxp3 gene may confer an important susceptibility to URSA in the Chinese Han population, probably by altering Foxp3 function and/or its expression.

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In vitrosuppression of natural killer cell cytolytic activities by prednisolone in patients with history of unexplained recurrent spontaneous abortion and elevated peripheral blood NK cell fraction

Korean Journal of Obstetrics & Gynecology ◽

10.5468/kjog.2011.54.7.361 ◽

2011 ◽

Vol 54 (7) ◽

pp. 361

Author(s):

Hyun Suk Ahn ◽

Sun Hwa Cha ◽

Hwa Seon Koo ◽

Ju Youn Bae ◽

Ji Hee Yoo ◽

...

Keyword(s):

Natural Killer Cell ◽

Natural Killer ◽

Spontaneous Abortion ◽

Peripheral Blood ◽

Nk Cell ◽

Killer Cell ◽

Cell Fraction ◽

Recurrent Spontaneous Abortion ◽

Unexplained Recurrent Spontaneous Abortion ◽

History Of

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Gut Microbiota Composition and Functional Prediction in Recurrent Spontaneous Abortion

10.21203/rs.3.rs-906730/v1 ◽

2021 ◽

Author(s):

Ying Cui ◽

Li Zou ◽

Qian Ye ◽

Dandan Li ◽

Huiming Wu ◽

...

Keyword(s):

Pregnant Women ◽

Spontaneous Abortion ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Recurrent Spontaneous Abortion ◽

Microbiota Composition ◽

Functional Prediction ◽

Significant Difference ◽

History Of ◽

Gut Microbiota Composition

Abstract Objective The changes of microbial community in pregnant women, let alone those of patients with recurrent spontaneous abortion (RSA), remain unclear. We analyzed the differences of gut mircobiota (GM) between RSA patients and pregnant women to find the possible mechanism of RSA. MethodsWe enrolled 30 RSA patients (RSA group) and 30 pregnant women who terminated their pregnancy and did not have a history of spontaneous abortion (NR group) in our hospital from June 2020 to August 2020, and fecal samples were obtained to analyze the GM using 16S rDNA V3–V4 sequencing.ResultsAt the phylum level, we found that there is no significant difference in composition of GM between RSA and NR. But at the genus level, compared with NR, Roseburia significantly decreased (P<0.01), and Ruminococcus significantly increased in RSA patients (P<0.05). Further analysis indicated that Klebsiella (P<0.05) was significantly increased, Prevotella.9 (P<0.05) and Roseburia (P<0.05) were significantly decreased in RSA2 group (BMI>23.9 in RSA). Moreover, Agathobacter (P<0.01) was significantly increased in NR2 group (no delivery in NR). Functional prediction indicated that GM may interfere with RSA through membrane transport, carbohydrate metabolism, amino acid metabolism and other pathways.ConclusionDecreased Roseburia in GM of pregnant women maybe related to RSA. Our results provide the basis for in-depth studies of the composition of gut microbial communities in RSA.

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Hypothyroidism In Women With Recurrent Spontaneous Abortion

Advances in Social Sciences Research Journal ◽

10.14738/assrj.710.9132 ◽

2020 ◽

Vol 7 (10) ◽

pp. 71-77

Author(s):

MOHAMED S. A. EMARAH ◽

MOHAMED A. EL-NAGGAR ◽

ABEER EL SHABACY ◽

SAHAR H. QUSHWA

Keyword(s):

Pregnant Women ◽

Spontaneous Abortion ◽

First Trimester ◽

Reproductive Age ◽

Recurrent Spontaneous Abortion ◽

Control Group ◽

Study Group ◽

Uterine Anomalies ◽

History Of ◽

Living Children

Recurrent miscarriage, defined as loss of two or more consecutive pregnancies, occurs in 1–2% of couples attempting to bear children. The major causes of recurrent pregnancy loss (RPL) based on the literature include parental structural chromosome rearrangement, immunologic factors (i.e. antiphospholipid syndrome), thrombophilic factors (both inherited and acquired), anatomic factors of uterine anomalies, and endocrinologic disorders. Luteal phase defect, polycystic ovarian syndrome (PCOS), diabetes mellitus, thyroid disease and hyperprolactinemia are among the endocrinologic disorders implicated in approximately 17% to 20% of RPL. The prevalence of hypothyroidism in the general population of reproductive age is about 2-3%. The aim of this study is to observe the benefit of screening for hypothyroidism amongst women with recurrent spontaneous abortion early in the first trimester. The study included one hundred and sixty (160) women, in the reproductive age of life, where there ages ranged from 20 – 33 years, and divided into two groups. Study group which included eighty (80), non pregnant women with a history of two or more consecutive spontaneous abortions early in the first trimester, with no living children and control group which included eighty (80), non pregnant women having one or more living children without any history of abortion. Hypothyroidism was noted in ten (10) cases (12.5%) in the study group and noted in two (2) cases (2.5%) in the control group with a statistically significant difference (P < 0.01). The mean levels of TSH in the study group was 22.71  13.13 Iu/ml. Conclusion: Screening for hypothyroidism has clinical significance and would help to reduce miscarriage rate in women with recurrent spontaneous abortion.

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