scholarly journals Metabolism of excised embryos of Lupinus luteus L. III. Comparative study of cultured embryos and normal seedling axes

2015 ◽  
Vol 31 (4) ◽  
pp. 693-702
Author(s):  
J. Czosnowski
Keyword(s):  
Comparative Study ◽  
Lupinus Luteus ◽  
Normal Seedling ◽  
Excised Embryos

scholarly journals Metabolism of excised embryos of Lupinus luteus L. VI. An electrophoretic analysis of some dehydrogenases in cultured embryos as compared with the normal seedling axes

2015 ◽  
Vol 43 (1) ◽  
pp. 117-127 ◽  
Author(s):  
J. Czosnowski
Keyword(s):  
Lactate Dehydrogenase ◽  
Glutamate Dehydrogenase ◽  
Electrophoretic Analysis ◽  
Disc Electrophoresis ◽  
Lupinus Luteus ◽  
The Third ◽  
Electrophoretic Patterns ◽  
Normal Seedling ◽  
Axial Organs ◽  
Excised Embryos

The electrophoretic patterns (disc electrophoresis) of the studied dehydrogenases: glucose-6-phosphate - (A), malate - (B), glutamate - (C), alcohol - (D) and lactate dehydrogenase (E), in the axial organs of isolated <i>Lupinus luteus</i> embryos and seedlings cultivated over 12 days are characterized by great similarities. With time, after the third day of cultivation the patterns begin to become less deyeloped. Analyses performed during the first 10 hours of imbibition of seed parts indicate that the maximal development of isozyme patterns occurs during the third hour after which the patterns become poorer. The most uniform type of pattern. and the lowest number of isozymes was shown by glutamate dehydrogenase, the richest pattern was shown by malate dehydrogenase. No band common for a 11 the 27 experimental elements was found.

scholarly journals Metabolism of excised embryos of Lupinus luteus L. VII. An electrophoretic analysis of esterases and peroxidases in cultured embryos and normal seedling axes

2015 ◽  
Vol 44 (1) ◽  
pp. 143-150 ◽  
Author(s):  
J. Czosnowski
Keyword(s):  
Strong Influence ◽  
Electrophoretic Analysis ◽  
Lupinus Luteus ◽  
Considerable Influence ◽  
General Development ◽  
The Third ◽  
Active Spectrum ◽  
Normal Seedling ◽  
Axial Organs ◽  
Excised Embryos

A rich and active spectrum of esterases (E) exists already in the organs of a dry seed. The isolation of axes from the cotyledons exerts a considerable influence on the pattern of E. In the axial organs of a seedling the spectra and activity become impoverished starting from the 3rd day of culture in the dark. On the other hand in the axial organs isolated from the cotyledons from that moment onwards the spectra become richer and more active, particularily in the root. It appears that the enhancement of spectra in the detached axes is not dependent on the elimination with the cotyledons of a source of inhibitors, since in the cotyledons, until the end of the culture period (12 days), a rich pattern of E is maintained. Peroxidase (P) appears starting from the third day giving impoverished spectra, in general development similar to E. The removal of the seed coat from dry seeds exerts during the first two days of imbibition a strong influence on the spectra of axial and cotyledonary enzyms.

scholarly journals A Comparative Study of Phase States of the Peribacteroid Membrane from Yellow Lupin and Broad Bean Nodules

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Natalia N. Kudryavtseva ◽  
Alexis V. Sof’in ◽  
Georgiy S. Bobylev ◽  
Evgeny M. Sorokin
Keyword(s):  
Comparative Study ◽  
Outer Membrane ◽  
Lipid Bilayer ◽  
Rhizobium Leguminosarum ◽  
Broad Bean ◽  
Lupinus Luteus ◽  
Bilayer Structure ◽  
Peribacteroid Membrane ◽  
Free Living ◽  
Yellow Lupin

A comparative study of the lipid bilayer phase status and structure of the outer membrane of free-livingBradyrhizobiumstrain 359a (Nod+Fix+) and 400 (Nod+FixL) orRhizobium leguminosarum97 (Nod+Fix+, effective) and 87 (Nod+FixL, ineffective) has been carried out. Also, the effect of the symbiotic pair combination on the lipid bilayer structure of the bacteroid outer membrane and peribacteroid membrane, isolated from the nodules ofLupinus luteusL. orVicia fabaL., has been studied. As a result, it is shown that the lipid bilayer status of the bacteroid outer membrane is mainly determined by microsymbiont, but not the host plant. In the contrast, the lipid bilayer status of the peribacteroid membrane and, as a consequence, its properties depend on interaction of both symbiotic partners.

scholarly journals Removing the endosperm of ginseng and American ginseng seeds results in embryos developing into normal seedlings

2020 ◽  
Vol 48 (2) ◽  
pp. 297-301
Author(s):  
Hongyu Li ◽  
Yuan Li ◽  
Shiquan Xu ◽  
Yingping Wang ◽  
Hao Zhang
Keyword(s):  
Seed Dormancy ◽  
Physiological Mechanism ◽  
Nutrient Deficiency ◽  
Culture Method ◽  
American Ginseng ◽  
Nutrient Level ◽  
Traditional Medicines ◽  
Normal Seedling ◽  
Excised Embryos

Both ginseng and American ginseng are world-famous traditional medicines with multiple bioactivities. The deep dormancy of their seeds causes serious problems in artificial cultivation. However, little is known about the physiological mechanism of seed dormancy and how to shorten the seed dormancy period for these two plant species. An experiment was conducted to determine whether endosperm removal would promote embryo development in ginseng and American ginseng and if in vitro embryos would suffer nutrient deficiency during seedling establishment. The results show that excised embryos developed radicles longer than 2 mm, using any culture method, whereas no germination was observed for intact seeds. Excised embryos, without the endosperm nutrient-supply have the ability of developing into a normal seedling, but in vitro embryos grown on MS medium have greater fresh weight, seedling height and radicle length than those grown on filter paper and pure agar medium. In summary, removing endosperm can overcome physiological dormancy of ginseng and American ginseng seeds, and nutrient level determines the development and growth rate of the embryo.

Author response for "Laminar distribution of cortical projection neurons to the pulvinar: A comparative study in cats and mice"

2020 ◽  
Author(s):  
Bruno Oliveira Ferreira de Souza ◽  
Éve‐Marie Frigon ◽  
Robert Tremblay‐Laliberté ◽  
Christian Casanova ◽  
Denis Boire
Keyword(s):  
Comparative Study ◽  
Author Response ◽  
Projection Neurons ◽  
Cortical Projection ◽  
Laminar Distribution
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