scholarly journals Metabolism of excised embryos of Lupinus luteus L. II. The water uptake as influenced by external concentration

2015 ◽  
Vol 31 (4) ◽  
pp. 683-691 ◽  
Author(s):  
J. Czosnowski
Keyword(s):  
Water Uptake ◽  
Lupinus Luteus ◽  
External Concentration ◽  
Excised Embryos

scholarly journals Metabolism of excised embryos of Lupinus luteus L. VI. An electrophoretic analysis of some dehydrogenases in cultured embryos as compared with the normal seedling axes

2015 ◽  
Vol 43 (1) ◽  
pp. 117-127 ◽  
Author(s):  
J. Czosnowski
Keyword(s):  
Lactate Dehydrogenase ◽  
Glutamate Dehydrogenase ◽  
Electrophoretic Analysis ◽  
Disc Electrophoresis ◽  
Lupinus Luteus ◽  
The Third ◽  
Electrophoretic Patterns ◽  
Normal Seedling ◽  
Axial Organs ◽  
Excised Embryos

The electrophoretic patterns (disc electrophoresis) of the studied dehydrogenases: glucose-6-phosphate - (A), malate - (B), glutamate - (C), alcohol - (D) and lactate dehydrogenase (E), in the axial organs of isolated <i>Lupinus luteus</i> embryos and seedlings cultivated over 12 days are characterized by great similarities. With time, after the third day of cultivation the patterns begin to become less deyeloped. Analyses performed during the first 10 hours of imbibition of seed parts indicate that the maximal development of isozyme patterns occurs during the third hour after which the patterns become poorer. The most uniform type of pattern. and the lowest number of isozymes was shown by glutamate dehydrogenase, the richest pattern was shown by malate dehydrogenase. No band common for a 11 the 27 experimental elements was found.

Water Relations of Tomato Seed Germination

1987 ◽  
Vol 14 (5) ◽  
pp. 485 ◽  
Author(s):  
AM Haigh ◽  
EWR Barlow
Keyword(s):  
Water Content ◽  
Water Uptake ◽  
Phase Ii ◽  
Phase Iii ◽  
Tomato Seed ◽  
Endosperm Tissue ◽  
Mechanical Restraint ◽  
Radicle Emergence ◽  
Uptake Studies ◽  
Excised Embryos

Water uptake during the germination of UC 82B tomato seeds was triphasic. Seed Ψ measurements indicated that phase I imbibition occurred because of the large Ψ gradient between the seed and the imbibition solution (water). During phase II the seed Ψ was in equilibrium with the water. Phase III water uptake recommenced with the onset of radicle emergence without changes in Ψ or Ψπ. Changes in embryo water content were also triphasic. During phase II the embryo Ψ remained at - 1.5 MPa, not in equilibrium with the imbibing solution. At radicle emergence it was - 0.8 MPa and rose to -0.3 MPa as the radicle elongated. There was no evidence of a lowering of embryo Ψπ nor of a build up of Ψp prior to radicle emergence. Water uptake studies with excised embryos indicated that, within the seed, the enclosing tissues prevented the embryo from taking up water. It is suggested that embryo water content is restricted by the constraint on embryo expansion caused by the enclosing endosperm tissue. Lowering of embryo Ψπ to build up Ψp is not necessary for radicle emergence. The control of germination may lie in the mechanism which leads to weakening of this mechanical restraint of the endosperm.

scholarly journals Metabolism of excised embryos of Lupinus luteus L. VII. An electrophoretic analysis of esterases and peroxidases in cultured embryos and normal seedling axes

2015 ◽  
Vol 44 (1) ◽  
pp. 143-150 ◽  
Author(s):  
J. Czosnowski
Keyword(s):  
Strong Influence ◽  
Electrophoretic Analysis ◽  
Lupinus Luteus ◽  
Considerable Influence ◽  
General Development ◽  
The Third ◽  
Active Spectrum ◽  
Normal Seedling ◽  
Axial Organs ◽  
Excised Embryos

A rich and active spectrum of esterases (E) exists already in the organs of a dry seed. The isolation of axes from the cotyledons exerts a considerable influence on the pattern of E. In the axial organs of a seedling the spectra and activity become impoverished starting from the 3rd day of culture in the dark. On the other hand in the axial organs isolated from the cotyledons from that moment onwards the spectra become richer and more active, particularily in the root. It appears that the enhancement of spectra in the detached axes is not dependent on the elimination with the cotyledons of a source of inhibitors, since in the cotyledons, until the end of the culture period (12 days), a rich pattern of E is maintained. Peroxidase (P) appears starting from the third day giving impoverished spectra, in general development similar to E. The removal of the seed coat from dry seeds exerts during the first two days of imbibition a strong influence on the spectra of axial and cotyledonary enzyms.

Water uptake by substituted amylose tablets: experimentation and numerical simulation

2009 ◽  
Vol 00 (00) ◽  
pp. 090904073309027-8
Author(s):  
H.W. Wang ◽  
S. Kyriacos ◽  
L. Cartilier
Keyword(s):  
Numerical Simulation ◽  
Water Uptake
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