Metabolism of excised embryos of Lupinus luteus L. I. Effect of metabolic inhibitors and growth substances on the water uptake

The electrophoretic patterns (disc electrophoresis) of the studied dehydrogenases: glucose-6-phosphate - (A), malate - (B), glutamate - (C), alcohol - (D) and lactate dehydrogenase (E), in the axial organs of isolated <i>Lupinus luteus</i> embryos and seedlings cultivated over 12 days are characterized by great similarities. With time, after the third day of cultivation the patterns begin to become less deyeloped. Analyses performed during the first 10 hours of imbibition of seed parts indicate that the maximal development of isozyme patterns occurs during the third hour after which the patterns become poorer. The most uniform type of pattern. and the lowest number of isozymes was shown by glutamate dehydrogenase, the richest pattern was shown by malate dehydrogenase. No band common for a 11 the 27 experimental elements was found.

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Water Relations of Tomato Seed Germination

Functional Plant Biology ◽

10.1071/pp9870485 ◽

1987 ◽

Vol 14 (5) ◽

pp. 485 ◽

Cited By ~ 28

Author(s):

AM Haigh ◽

EWR Barlow

Keyword(s):

Water Content ◽

Water Uptake ◽

Phase Ii ◽

Phase Iii ◽

Tomato Seed ◽

Endosperm Tissue ◽

Mechanical Restraint ◽

Radicle Emergence ◽

Uptake Studies ◽

Excised Embryos

Water uptake during the germination of UC 82B tomato seeds was triphasic. Seed Ψ measurements indicated that phase I imbibition occurred because of the large Ψ gradient between the seed and the imbibition solution (water). During phase II the seed Ψ was in equilibrium with the water. Phase III water uptake recommenced with the onset of radicle emergence without changes in Ψ or Ψπ. Changes in embryo water content were also triphasic. During phase II the embryo Ψ remained at - 1.5 MPa, not in equilibrium with the imbibing solution. At radicle emergence it was - 0.8 MPa and rose to -0.3 MPa as the radicle elongated. There was no evidence of a lowering of embryo Ψπ nor of a build up of Ψp prior to radicle emergence. Water uptake studies with excised embryos indicated that, within the seed, the enclosing tissues prevented the embryo from taking up water. It is suggested that embryo water content is restricted by the constraint on embryo expansion caused by the enclosing endosperm tissue. Lowering of embryo Ψπ to build up Ψp is not necessary for radicle emergence. The control of germination may lie in the mechanism which leads to weakening of this mechanical restraint of the endosperm.

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Metabolism of excised embryos of Lupinus luteus L. III. Comparative study of cultured embryos and normal seedling axes

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1962.048 ◽

2015 ◽

Vol 31 (4) ◽

pp. 693-702

Author(s):

J. Czosnowski

Keyword(s):

Comparative Study ◽

Lupinus Luteus ◽

Normal Seedling ◽

Excised Embryos

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Residual Effects of Auxin, Chelating Agents, and Metabolic Inhibitors in Cell Extension

Australian Journal of Biological Sciences ◽

10.1071/bi9590373 ◽

1959 ◽

Vol 12 (4) ◽

pp. 373 ◽

Cited By ~ 10

Author(s):

DJ Carr ◽

EK Ng

Keyword(s):

Water Uptake ◽

Chelating Agents ◽

Residual Effects ◽

Metabolic Inhibitors ◽

Wheat Coleoptile ◽

Cell Extension ◽

Aerobic Conditions ◽

Auxin Action ◽

Growth Promoting

The growth-promoting action of auxin on wheat coleoptile segments can be separated from the act of cellular expansion by use of the technique of Cleland and Bonner (1956). The effects of a pretreatment with auxin ("residual effects") in hypertonic mannitol are manifested later when the coleoptiles are transferred to water. Auxin action, but not water uptake, requires aerobic conditions.

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