scholarly journals Phases of crown-gall transformation susceptible to hydroxyurea

2014 ◽  
Vol 53 (1) ◽  
pp. 59-65 ◽  
Author(s):  
Aldona Rennert ◽  
Józef Kowalczyk ◽  
Halina Pajkowska
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Pathogenic Bacteria ◽  
Direct Action ◽  
Plant Cells ◽  
Induction Phase ◽  
Bacterial Strains ◽  
Tumour Formation ◽  
Kalanchoë Daigremontiana ◽  
Anti Tumour Effect

With the use of bacterial strains, both sensitive and resistant to hydroxyurea the action of this inhibitor on tumour formation on the leaves of <em>Kalanchoe daigremontiana</em> infected with <em>Agrobacterium tumefaciens</em> was tested for five days after inoculation. The results are in agreement with the opinion that the anti-tumour effect of hydroxyurea applied in the induction phase (between 18 and 60 h after inoculation) is the result of its direct action on plant cells, whereas inhibition of tumour formation by the inhibitor in the inoculation period depends on its action on the pathogenic bacteria.

scholarly journals EFFICACY OF BIOCHEMICAL PREPARATIONS AND EXTRACT FROM Hypericum perforatum AGAINST BACTERIAL DISEASES

2019 ◽  
Vol 18 (3) ◽  
Author(s):  
Małgorzata Schollenberger ◽  
Sylwia Pudło ◽  
Elżbieta Paduch-Cichal ◽  
Ewa Mirzwa-Mróz
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Pseudomonas Syringae ◽  
Pathogenic Bacteria ◽  
Hypericum Perforatum ◽  
Agar Plate ◽  
Nutrient Agar ◽  
Bacterial Strains ◽  
Bacterial Diseases ◽  
Nutrient Agar Medium

The biotechnical preparations: Biosept Active (based on a grapefruit extract) and BioZell (based on thyme oil) as well as Hypericum perforatum extract, streptomycin solution and fungicide Champion 50WP (active ingredient substance – e.i. 50% copper hydroxide) were investigated for antimicrobial effects against plant pathogenic bacteria: Agrobacterium tumefaciens, Pseudomonas syringae pv. syringae and Xanthomonas ar- boricola pv. corylina. The screening was carried out in vitro on three media: Nutrient Agar (NA Difco), Pseudomonas Agar F (Merck) – analogue of King B and 523. In the experiments, the agar plate method was applied. There were no statistically significant differences in the effect of streptomycin and Champion 50WP on the growth inhibition of three bacteria strains for medium 523 and Nutrient Agar and of P. syringae pv. syringae and X. arboricola pv. corylina for medium King B. It was determined that the antibacterial activity of Biosept Active and BioZell biopreparations and H. perforatum extract against Agrobacterium tumefaciens (strain C58), Pseudomonas syringae pv. syringae (strain 760) and Xanthomonas arboricola pv. corylina (strain RIPF-x13) were dependent on the strain of pathogen as well as the growth medium used. According to the research results obtained, the Biosept Active preparation and H. perforatum extract demonstrated high bacteriostatic activity against three bacterial strains grown on the Nutrient Agar medium.

Untersuchungen an Crown-Gall

1970 ◽  
Vol 25 (7) ◽  
pp. 735-738 ◽  
Author(s):  
Rolf Beiderbeck
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Tumor Formation ◽  
Tumor Induction ◽  
Host System ◽  
Induced Tumors ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

Concentrations of 10 µg/ml rifampicin reduce the growth of Agrobacterium tumefaciens by more than 90%, concentrations of 100 µg/ml prevent tumor formation in Kalanchoë daigremontiana by the same bacteria.A treatment with rifampicin does not irreversibly alter the bacteria; after removal of the drug bacterial propagation and the ability of tumor induction are restored.0-24 h treatments of the plant wounds with rifampicin prior to infection prevent tumor formation completely.Addition of rifampicin after infection inhibits tumor formation optimally if the drug is added only few hours after inoculation. 30 h after infection the bacteria-host-system is resistant against rifampicin.100 μg/ml rifampicin do not influence the growth of already induced tumors.

Identification of the Agrobacterium tumefaciens C58 T-DNA genes e and f and their impact on crown gall tumour formation

1995 ◽  
Vol 27 (1) ◽  
pp. 41-57 ◽  
Author(s):  
I. Broer ◽  
W. Dr�ge-Laser ◽  
R. F. Barker ◽  
K. Neumann ◽  
W. Klipp ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Tumour Formation ◽  
Crown Gall Tumour ◽  
Agrobacterium Tumefaciens C58

scholarly journals Effect of N-hydroxyurea, mitomycin C and actinomycin D on tumour formation on the leaves of Kalanchoe daigremontiana

2014 ◽  
Vol 52 (1) ◽  
pp. 61-69 ◽  
Author(s):  
Józef Koawalczyk
Keyword(s):  
Dna Synthesis ◽  
Actinomycin D ◽  
Plant Cells ◽  
Host Tissue ◽  
Host Cells ◽  
Tumour Formation ◽  
Second Period ◽  
Dna Synthesis Inhibitors ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

The leaves of <em>Kalanchoe daigremontiana</em> wounded and infected with <em>Agrobacterium tumefaciens</em> were treated with single doses of inhibitors (hydroxyurea - 190, mitomycin - 0.5, actinomycin - 2 µ,g per leaf). After delaying the time' of dosage of inhibitors during five days after inoculation, changes in susceptibility of the system to antitumorous activity of analysed compounds were observed. In several hours after inoculation (period of the bacteria metabolic activity in wounds) all the inhibitors prevent strongly the tumour formation. At the time between 14 and 72 hours after inoculation, including the phase of tumour induction, the system becomes sensitive to the DNA synthesis inhibitors, particularly hydroxyurea. The intensified action of actinomycin appears again only about 60 hours after inoculation and lasts till the end of experiment (the initiation of the transformed plant cell proliferation). According to the literature the antitumorous effect of inhibitors could be connected with their action on the bacteria metabolism inside the host tissue. The activities of hydroxyurea and mitomycin in the second period correspond with the intensive DNA synthesis in plant cells, which is induced by wounding. The effect of actinomycin D in 60 hours after inoculation could depend upon the inhibition of the proliferation of the transformed host cells.

scholarly journals Identification and Characterization of a Pseudomonas mosselii strain and its Antibacterial Function against Agrobacterium tumefaciens

2021 ◽  
Vol 4 (2) ◽  
pp. 223-231
Author(s):  
Zhilong Wang ◽  
◽  
Wen Li ◽  
Jiaying Wang ◽  
Xintong Jiang ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Biochemical Characterization ◽  
Inhibition Zone ◽  
Tumor Formation ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Cherry Tree ◽  
Bacterial Colony ◽  
Crown Gall Disease

Agrobacterium tumefaciens is a gram negative bacterial that can infect a range of plants and result in root crown gall. A total number of 10328 bacterial strains were isolated from rhizosphere of cherry tree. One strain of LWB10 showed clear inhibition zone around the bacterial colony in YEB media inoculated with A. tumefaciens C58. Morphological, physiological, and biochemical characterization indicated that LWB10 belongs to member of the genus Pseudomonas. Results from the high-throughput matrix-assisted laser desorption/ionization biotypersmart system indicated that this strain had a score value of 2.247 relative to Pseudomonas mosselii. Also, phylogenetic analysis based on 16S rRNA gene sequence showed that strain LWB10 shared the highest similarity with Pseudomonas mosselii CIP 105259T. The antagonist strains also exhibit well in growth inhibition of other five A. tumefaciens strains. Coinoculation of LWB10 and plant pathogenic strain of A. tumefaciens CFCC1369 showed strongly inhibition of tumor formation in tomato stems. All the results demonstrated that the isolated strain is P. mosselii LWB10 and its antibacterial ability to A. tumefaciens may offer new way for management of crown gall disease in the future. Keywords: Agrobacterium tumefaciens, Pseudomonas mosselii, antagonist, biological control

scholarly journals Influence of N-hydroxyurea on the growth of seedlings and the process of crown-gall tumour formation on sunflower plants

2015 ◽  
Vol 47 (1–2) ◽  
pp. 51-63 ◽  
Author(s):  
Aldona Rennert
Keyword(s):  
Direct Action ◽  
Transformation Process ◽  
Host Cells ◽  
Test Plant ◽  
Induction Phase ◽  
Experimental Conditions ◽  
Tumour Formation ◽  
Activity Curve ◽  
Crown Gall Tumour ◽  
Growth Of Seedlings

Hydroxyurea (HU) strongly inhibits formation of tumours induced with <i>Agrobacterium tumefaciens</i> in sunflower stems. This effect may partly be ascribed to the direct action of this substance on the bacterium. The course of the HU activity curve in the transformation process leads, however, to the supposition that it acts mainly on the host cells at the time corresponding to the induction phase. Maximal plant cell susceptibility to HU coincides with the wave of DNA synthesis induced by injury to the plant. Under the experimental conditions the time of HU activity in the tissues of the control test plant was limited, and the effects receded during its growth and development.

scholarly journals VirE1-Mediated Resistance to Crown Gall in Transgenic Arabidopsis thaliana

2006 ◽  
Vol 96 (1) ◽  
pp. 105-110 ◽  
Author(s):  
Jodi Humann ◽  
Sarah Andrews ◽  
Walt Ream
Keyword(s):  
Arabidopsis Thaliana ◽  
Agrobacterium Tumefaciens ◽  
Crown Gall ◽  
Plant Cells ◽  
Bacterial Cells ◽  
Root Explants ◽  
Single Stranded Dna ◽  
Virulence Proteins ◽  
Crown Gall Disease ◽  
Efficient Transmission

Crown gall disease, caused by Agrobacterium tumefaciens, remains a serious agricultural problem despite current biocontrol methods. Agrobacterium tumefaciens transfers single-stranded DNA (T-strands) into plant cells along with several virulence proteins, including a single-stranded DNA-binding protein (VirE2). In plant cells, T-strands are protected from nucleases and targeted to the nucleus by VirE2, which is essential for efficient transmission (transfer and integration) of T-strands. VirE1 is the secretory chaperone for VirE2; it prevents VirE2 from forming aggregates and from binding the T-strands in bacterial cells. Therefore, we hypothesized that sufficient quantities of VirE1 expressed in plant cells might block T-DNA transmission by preventing VirE2 from binding T-strands. Here we show that root explants from Arabidopsis thaliana plants that expressed virE1 formed 3.5-fold fewer tumors than roots from plants without virE1. Also, this resistance was specific for VirE2-mediated Agrobacterium transformation. Plants that have been genetically altered to resist crown gall may prove more effective than biological control.

Untersuchungen an Crown-Gall / Investigations on Crown-Gall

1970 ◽  
Vol 25 (4) ◽  
pp. 407-411 ◽  
Author(s):  
Rolf Beiderbeck
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Quantitative Evaluation ◽  
Crown Gall ◽  
Equal Size ◽  
Tumor Diameter ◽  
Relative Success ◽  
Induced Tumors ◽  
Young Leaves ◽  
Kalanchoë Daigremontiana ◽  
Kalanchoe Daigremontiana

Young leaves of Kalanchoe daigremontiana are hurt with a “hedgehog” (a holder with 30 needles) to produce wounds of equal size in great numbers and definite intervals. Infection of these leaves with suspensions of Agrobacterium tumefaciens, strain B6, results in first small tumors as soon as 7 days after infection. A quantitative evaluation of the experiments is possible 14 days after infection. The number of induced tumors increases linearly with the logarithm of the concentration of the bacteria. The diameter of tumors (21-d-old), too, depends on the concentration of the bacteria. - The tumors mass a product of the relative success of infection I (number of tumors per number of wounds, and the average tumor diameter gives a third measure of the bacteria concentration.An application of this hedgehog-test is given: If the infecting bacteria are treated with lysozyme and EDTA the success of the infection decreases with increasing time of treatment. This decrease is reduced by the presence of 11% sucrose.

scholarly journals Plant Transformation by Coinoculation with a Disarmed Agrobacterium tumefaciens Strain and an Escherichia coli Strain Carrying Mobilizable Transgenes

2003 ◽  
Vol 69 (11) ◽  
pp. 6731-6739 ◽  
Author(s):  
Katherine M. Pappas ◽  
Stephen C. Winans
Keyword(s):  
Escherichia Coli ◽  
Agrobacterium Tumefaciens ◽  
Plant Cells ◽  
Leaf Explants ◽  
Coli Strain ◽  
Broad Host Range ◽  
Transfer System ◽  
Bacterial Strains ◽  
Conjugation System ◽  
E Coli

ABSTRACT Transformation of Nicotiana tabacum leaf explants was attempted with Escherichia coli as a DNA donor either alone or in combination with Agrobacterium tumefaciens. We constructed E. coli donor strains harboring either the promiscuous IncP-type or IncN-type conjugal transfer system and second plasmids containing the respective origins of transfer and plant-selectable markers. Neither of these conjugation systems was able to stably transform plant cells at detectable levels, even when VirE2 was expressed in the donor cells. However, when an E. coli strain expressing the IncN-type conjugation system was coinoculated with a disarmed A. tumefaciens strain, plant tumors arose at high frequencies. This was caused by a two-step process in which the IncN transfer system mobilized the entire shuttle plasmid from E. coli to the disarmed A. tumefaciens strain, which in turn processed the T-DNA and transferred it to recipient plant cells. The mobilizable plasmid does not require a broad-host-range replication origin for this process to occur, thus reducing its size and genetic complexity. Tumorigenesis efficiency was further enhanced by incubation of the bacterial strains on medium optimized for bacterial conjugation prior to inoculation of leaf explants. These techniques circumvent the need to construct A. tumefaciens strains containing binary vectors and could simplify the creation of transgenic plants.

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