scholarly journals Studies on Medicago lupulina saponins. 1. Isolation and identification of sapogenins from M. lupulina tops

2014 ◽  
Vol 53 (4) ◽  
pp. 515-525 ◽  
Author(s):  
Piotr M. Górski ◽  
Marian Jurzysta ◽  
Stanisław Burda ◽  
Wiesław A. Oleszek ◽  
Michał Płoszyński
Keyword(s):  
Mass Spectrometry ◽  
Methyl Ester ◽  
Ester Group ◽  
Infrared Spectrometry ◽  
Isolation And Identification ◽  
Medicago Lupulina ◽  
Chromatography Mass Spectrometry ◽  
Medicagenic Acid ◽  
First Time

Saponins from <em>M. lupulina</em> tops were investigated for the first time. Eight aglycones were found in the acid hydrolysates of saponins. All of the aglycones were isolated. On the basis of chromatography, mass spectrometry and infrared spectrometry the aglycanes were identified as soyasapogenols B, C, D, E, F and medicagenic acid. Two new aglycones were also isolated and identified as pentacyclic triterpens of β-amyrin structure. Both possess a methyl ester group which is rarely present in nature.

Identification by Gas Chromatography-Mass Spectrometry of 150 Compounds in Propolis

1991 ◽  
Vol 46 (1-2) ◽  
pp. 111-121 ◽  
Author(s):  
W. Greenaway ◽  
J. May ◽  
T. Scaysbrook ◽  
F. R. Whatley
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Alcoholic Extract ◽  
Headspace Volatiles ◽  
Chromatography Mass Spectrometry ◽  
First Time

Abstract Propolis was analyzed by gas chromatography-mass spectrometry for both its headspace volatiles and for the less volatile components of its alcoholic extract (propolis balsam). 181 peaks were located of which 171 representing 150 compounds were identified, including 28 identified in propolis for the first time. The majority of compounds were typical of poplar bud exudate.

Comprehensive analysis of metabolites in Corynebacterium glutamicum by gas chromatography/mass spectrometry

2004 ◽  
Vol 385 (9) ◽  
pp. 853-861 ◽  
Author(s):  
Sergey Strelkov ◽  
Mirko von Elstermann ◽  
Dietmar Schomburg
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Corynebacterium Glutamicum ◽  
Growth Conditions ◽  
Gas Chromatography Mass Spectrometry ◽  
Fast Method ◽  
Chromatography Mass Spectrometry ◽  
High Matrix ◽  
First Time ◽  
Derivatives Of

AbstractAn analytical method based on gas chromatography/mass spectrometry was developed for metabolome investigation ofCorynebacterium glutamicum. For the first time a fast method for metabolic screening that can be automated is described for this organism. More than 1000 compounds could be detected per experiment, ca. 330 of those showed a peak area significantly above background. Out of these 164 compounds were identified so far, representing derivatives of 121 different metabolites, which were quantified in one sample. In spite of the different chemical nature of metabolites and high matrix content, a measurement reproducibility in the range of 6% error was achieved. The application of this method for the analysis of the adaptation ofC. glutamicumto different growth conditions is demonstrated.

scholarly journals Identification of Pyrrolizidine Alkaloids in Senecio Plants by Liquid Chromatography-Mass Spectrometry

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
An-Jing Lu ◽  
Yan-Liu Lu ◽  
Dao-Peng Tan ◽  
Lin Qin ◽  
Hua Ling ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Pyrrolizidine Alkaloids ◽  
Liquid Chromatography Mass Spectrometry ◽  
Research Information ◽  
Chemical Research ◽  
Chromatography Mass Spectrometry ◽  
Product Ions ◽  
First Time ◽  
Weight Number

Pyrrolizidine alkaloids (PAs) are considered as the major constituents that cause hepatoxicity in Senecio plants. PAs can be found in about 3%–5% of the world’s flowering plants. Nowadays, the identification method of PAs by separation and preparation was too slow and lacked effective power. A rapid method to identify PAs in plants must be developed. Based on the fragmentation regularity, the hepatoxic PAs and nonhepatoxic PAs were characterized by liquid chromatography-mass spectrometry (LC-MS). The detailed structures of PAs in five Senecio plants were identified based on tandem mass spectrometry (MS/MS) spectrum and chemical research information. In the present study, some new fragmentation regularities of PAs have been found, such as product ions at m/z 122, m/z 140 and m/z 124, m/z 142, which have been discovered as the characteristic fragments of lactone and mono-esterase type of saturated PAs, respectively. Moreover, two product ions at m/z 120 and m/z 138 have been reported as the characteristic fragments of unsaturated PAs. Some of them were found in Senecio species for the first time, and some of them may be new nature product or even new compound. Finally, we classified these plants into five categories based on PAs which were identified in the present study; the result corresponded with the classification by morphology. In addition, we have found some constituents that have odd molecular weight number only in Senecio species but not in Ligularia species; the detailed structures of these non-PAs constituents need penetrating study. LC-MS was rapid and sensitive method for detecting and identifying PAs in plants. Pyrrolizidine alkaloids were the toxiferous constituent of Senecio plants. In this study, we found that PAs can be used as the characteristic constituent of Senecio species.

scholarly journals A validated method for the analysis and profiling of ‘nyaope’ using gas chromatography – mass spectrometry

2021 ◽  
Vol 117 (11/12) ◽  
Author(s):  
Pabalala M. Mthembi ◽  
Ellen M. Mwenesongole ◽  
Michael D. Cole
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Principal Component ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Drug Cocktail ◽  
Spectrometry Analysis ◽  
Chromatography Mass Spectrometry ◽  
First Time ◽  
Problem Drug

Nyaope, a Tswana word for a mixture or ‘mish-mash’, describes a drug cocktail consisting of heroin, cannabis, and on occasion other controlled substances and warfarin. It is highly addictive with extremely unpleasant side effects caused by withdrawal from the drug. It is a problem drug especially in townships in South Africa. However, its prevalence in neighbouring southern African states and further afield is not yet known. There is currently no validated method for the analysis and comparison of nyaope. We describe a validated method for the gas chromatography – mass spectrometry analysis of nyaope so that within-batch and between-batch comparisons of nyaope can successfully be made for the first time. The validated method managed an accuracy within the range 80–120%, the precision was less than 20% for all analytes and managed linearity with R2≥0.99. The detection limits for diamorphine, efavirenz, nevirapine and Δ9-tetrahydrocannabinol were 14.2, 18.6, 18.7 and 9.94 pg on column, respectively, and the limits of quantitation were 43.1, 56.3, 56.6 and 30.1 pg on column, respectively. The simulated and casework samples were successfully discriminated into original batches using the identified nyaope components, the unsupervised chemometric methods principal component analysis and hierarchical clustering, as well as chromatographic profiles.

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