Isolation, chemical characterization and biological activity of alfalfa (Medicago media Pers.) root saponins

Saponins from alfalfa (Medicago media Pers.) roots were isolated and their acid hydrolysis revealed several aglycones that were obtained in crystalline form and characterized. Medicagenic acid, hederagenin and soyasapogenols A. B, C. D, E and F were found. Crude saponins were separated into cholesterol-precipitable and nonprecipitable fractions. The precipitable fraction consisted of medicagenic acid glycosides: glucose, arabinose, xylose and rhamnose were found as their sugar chain components. The nonprecipitable fraction was a mixture of hederagenine and soyasapogenol glycosides, and glucose arthinose. xylose. galactose and glucuronic acid were found in the sugar component,. 7 he medicagenic acid glycosides made up 6% of root dry matter and showed high biological activity. They cuased red blood cells lysis (haemolytic index 3000), completely inhibited Trichoderma viride growth at the concentration of 2.5 Mg. 100 cm-3 of growth medium and retarded wheat seedling growth at concentrations as low as 100 ppm. The cholesterol-nonprecipitable fraction caused no blood cell lysis and fungus growth inhibition, although it did inhibit seedling growth, but to a much lesser extent than medicagenic acrd glycosides. The detrimental effect of alfalfa root saponins on winter wheat crop after alfalfa is discussed.

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Studies on Medicago lupulina saponins. 2. Isolation, chemical characterization and biological activity of saponins from M. lupulina tops

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1984.048 ◽

2014 ◽

Vol 53 (4) ◽

pp. 527-533 ◽

Cited By ~ 6

Author(s):

Piotr M. Górski ◽

Marian Jurzysta ◽

Stanisław Burda ◽

Wiesław A. Oleszek ◽

Michał Płoszyński

Keyword(s):

Biological Activity ◽

Glucuronic Acid ◽

Chemical Characterization ◽

Medicago Lupulina ◽

Medicagenic Acid

Two pure saponin fractions from the tops of M. lupulina were isolated and characterized. The fractions named Ma and Ss varied in composition and biological activity. It was shown by TLC that fraction Ma contained 5 components. In the acid hydrolysates of that fraction only one aglycone (medicagenic acid) and 4 sugars: rhamnose, xylose, arabinose, glucuronic acid were found. The Ss fraction contained 11 components. In its acid hydrolysates, soyasapogenols B, C, D, E, F and two new aglycones N and An were found. The same sugars as above and additionally glucose and galactose were identified. It was found that the Ma fraction of saponins (medicagenic acid glycosides) was highly fungistatic, haemolytic and toxic to fishes. The Ss fraction of saponins (soyasapogenol glycosides) showed no such activity.

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Biological Activity of the Nanostructure Poly(Ester-Imide)s Containing a Tyrosine-Based Diol: Wheat Seedling Growth and Fungal Biodegradation

Polymer-Plastics Technology and Engineering ◽

10.1080/03602559.2013.844834 ◽

2014 ◽

Vol 53 (5) ◽

pp. 459-464 ◽

Cited By ~ 2

Author(s):

Shadpour Mallakpour ◽

Fatemeh Zeraatpisheh ◽

Mohammad R. Sabzalian

Keyword(s):

Biological Activity ◽

Seedling Growth ◽

Wheat Seedling ◽

Fungal Biodegradation

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Isolation, chemical characterization and biological activity of red clover (Trifolium pratense L.) root saponins

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1986.025 ◽

2014 ◽

Vol 55 (2) ◽

pp. 247-252 ◽

Cited By ~ 8

Author(s):

Wiesław Oleszek ◽

Marian Jurzysta

Keyword(s):

Biological Activity ◽

Trifolium Pratense ◽

Glucuronic Acid ◽

Chemical Characterization ◽

Red Clover ◽

Wheat Seedling ◽

Fungistatic Activity ◽

Water Suspensions ◽

Trifolium Pratense L ◽

Hydrolysis Of

Crystalline saponins were isolated from red clover (Trifolium pratense L.) roots. They were a mixture of two glycosides showing no haemolytic or fungistatic activity. Acid hydrolysis of these saponins yielded soyasapogenols B. C. D. E and F and rhamnose. xylose, arabinose glucose and glucuronic acid as sugar components. They were poorly or not soluble in water and well soluble in ethanol. Water suspensions of these saponins did not affect winter wheat seedling growth.

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A FACILE SYNTHESIS AND REACTIONS OF AMINO SELENOLO[2,3-b]PYRIDINE CARBOXYLATE

JOURNAL OF ADVANCES IN CHEMISTRY ◽

10.24297/jac.v12i1.845 ◽

2015 ◽

Vol 12 (1) ◽

pp. 3910-3918 ◽

Cited By ~ 1

Author(s):

Dr Remon M Zaki ◽

Prof Adel M. Kamal El-Dean ◽

Dr Nermin A Marzouk ◽

Prof Jehan A Micky ◽

Mrs Rasha H Ahmed

Keyword(s):

Biological Activity ◽

Carbonyl Compounds ◽

Mass Spectra ◽

The Other ◽

Pyridine Derivatives ◽

Facile Synthesis ◽

High Biological Activity ◽

Basic Hydrolysis ◽

Pyridine Carboxylate ◽

Hydrolysis Of

Incorporating selenium metal bonded to the pyridine nucleus was achieved by the reaction of selenium metal with 2-chloropyridine carbonitrile 1 in the presence of sodium borohydride as reducing agent. The resulting non isolated selanyl sodium salt was subjected to react with various α-halogenated carbonyl compounds to afford the selenyl pyridine derivatives 3a-f which compounds 3a-d underwent Thorpe-Ziegler cyclization to give 1-amino-2-substitutedselenolo[2,3-b]pyridine compounds 4a-d, while the other compounds 3e,f failed to be cyclized. Basic hydrolysis of amino selenolo[2,3-b]pyridine carboxylate 4a followed by decarboxylation furnished the corresponding amino selenolopyridine compound 6 which was used as a versatile precursor for synthesis of other heterocyclic compound 7-16. All the newly synthesized compounds were established by elemental and spectral analysis (IR, 1H NMR) in addition to mass spectra for some of them hoping these compounds afforded high biological activity.

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The Psychonauts’ Benzodiazepines; Quantitative Structure-Activity Relationship (QSAR) Analysis and Docking Prediction of Their Biological Activity

Pharmaceuticals ◽

10.3390/ph14080720 ◽

2021 ◽

Vol 14 (8) ◽

pp. 720

Author(s):

Valeria Catalani ◽

Michelle Botha ◽

John Martin Corkery ◽

Amira Guirguis ◽

Alessandro Vento ◽

...

Keyword(s):

Biological Activity ◽

Computational Models ◽

Quantitative Structure Activity Relationship ◽

Structure Activity Relationship ◽

Docking Studies ◽

Health Concern ◽

Activity Relationship ◽

Quantitative Structure ◽

High Biological Activity ◽

Structure Activity

Designer benzodiazepines (DBZDs) represent a serious health concern and are increasingly reported in polydrug consumption-related fatalities. When new DBZDs are identified, very limited information is available on their pharmacodynamics. Here, computational models (i.e., quantitative structure-activity relationship/QSAR and Molecular Docking) were used to analyse DBZDs identified online by an automated web crawler (NPSfinder®) and to predict their possible activity/affinity on the gamma-aminobutyric acid A receptors (GABA-ARs). The computational software MOE was used to calculate 2D QSAR models, perform docking studies on crystallised GABA-A receptors (6HUO, 6HUP) and generate pharmacophore queries from the docking conformational results. 101 DBZDs were identified online by NPSfinder®. The validated QSAR model predicted high biological activity values for 41% of these DBDZs. These predictions were supported by the docking studies (good binding affinity) and the pharmacophore modelling confirmed the importance of the presence and location of hydrophobic and polar functions identified by QSAR. This study confirms once again the importance of web-based analysis in the assessment of drug scenarios (DBZDs), and how computational models could be used to acquire fast and reliable information on biological activity for index novel DBZDs, as preliminary data for further investigations.

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Replacement of the interchain disulfide bridge-forming amino acids A7 and B7 by glutamate impairs the structure and activity of insulin

Biological Chemistry ◽

10.1515/bc.2004.151 ◽

2004 ◽

Vol 385 (12) ◽

pp. 1171-1175 ◽

Cited By ~ 7

Author(s):

Zhan-Yun Guo ◽

Xiao-Yuan Jia ◽

You-Min Feng

Keyword(s):

Biological Activity ◽

Disulfide Bonds ◽

Negative Charge ◽

Disulfide Bridge ◽

Site Directed Mutagenesis ◽

Side Chain ◽

Insulin Analogs ◽

High Biological Activity ◽

Chemical Groups ◽

Structure And Activity

Abstract Insulin contains three disulfide bonds, one intrachain bond, A6–A11, and two interchain bonds, A7–B7 and A20–B19. Site-directed mutagenesis results (the two cysteine residues of disulfide A7–B7 were replaced by serine) showed that disulfide A7–B7 is crucial to both the structure and activity of insulin. However, chemical modification results showed that the insulin analogs still retained relatively high biological activity when A7Cys and B7Cys were modified by chemical groups with a negative charge. Did the negative charge of the modification groups restore the loss of activity and/or the disturbance of structure of these insulin analogs caused by deletion of disulfide A7–B7? To answer this question, an insulin analog with both A7Cys and B7Cys replaced by Glu, which has a long side-chain and a negative charge, was prepared by protein engineering, and its structure and activity were analyzed. Both the structure and activity of the present analog are very similar to that of the mutant with disulfide A7–B7 replaced by Ser, but significantly different from that of wild-type insulin. The present results suggest that removal of disulfide A7–B7 will result in serious loss of biological activity and the native conformation of insulin, even if the disulfide is replaced by residues with a negative charge.

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